| characterization of two novel haloalkaliphilic archaea natronorubrum bangense gen. nov., sp. nov. and natronorubrum tibetense gen. nov., sp. nov. | two haloalkaliphilic archaea were isolated from a soda lake in tibet. the two strains, designated a33t and ga33t, were gram-negative, pleomorphic, flat, non-motile and strictly aerobic. growth required at least 12% nacl. growth was between ph 8.0 and ph 11 with an optimum at ph 9.0-9.5. cells were chemo-organotrophic. polar lipids were c20-c25 derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate. the nucleotide sequences of the 16s rrna genes from the two strains were obtained ... | 1999 | 10028271 |
| growth kinetics of extremely halophilic archaea (family halobacteriaceae) as revealed by arrhenius plots. | members of the family halobacteriaceae in the domain archaea are obligate extreme halophiles. they occupy a variety of hypersaline environments, and their cellular biochemistry functions in a nearly saturated salty milieu. despite extensive study, a detailed analysis of their growth kinetics is missing. to remedy this, arrhenius plots for 14 type species of the family were generated. these organisms had maximum growth temperatures ranging from 49 to 58 degrees c. nine of the organisms exhibited ... | 2005 | 15659670 |
| oxidation of thiosulfate to tetrathionate by an haloarchaeon isolated from hypersaline habitat. | a novel, extremely halophilic, neutrophilic archaeon was isolated from a mixed sediment sample from different hypersaline lakes in kulunda steppe (altai, russia) at 4 m nacl with acetate and thiosulfate as substrates. the enrichment culture developed in two phases. during the first phase, a rapid growth of heterotrophic, red-colored, polymorphic rods occurred with the concomitant oxidation of thiosulfate to tetrathionate. the latter was subsequently oxidized to sulfate during a second, slower ph ... | 2005 | 16041477 |
| [archaeal diversity of zabuye lake in tibet analyzed by culture-independent approach]. | with culture-independent approach, microbial dna was directly extracted from samples of zabuye saline soda lake. using the microbial dna as template, archaeal 16s rdnas were amplified by pcr. amplified products were cloned and sequenced. 60 different cloned partial sequences, most of which were related to haloalkaliphilic archaeon, were acquired. in the phylogenetic tree, some clones of zabuye lake belonged to genus natronobacterium, natrinema, natronococcus, natronorubrum, natronomonas, halorub ... | 2003 | 16276911 |
| [diversity of halophilic archaea in hypersaline lakes of inner mongolia, china]. | the aims of this work were to explore the diversity of halophilic archaea in hypersaline lakes of inner mongolia, china and to collect novel halophilic archaea. one hundred and sixty-five halophilic archaea were isolated from the three different types of hypersaline lakes (erliannor, shangmatala and xilin soda lake) in inner mongolia. by analysis of the restriction patterns of amplified 16s rdna (ardra) with the enzyme afa i and hae ii, respectively, the isolates were clustered into 14 genotypes ... | 2006 | 16579455 |
| [biodiversity of halophilic archaea isolated from two salt lakes in xin-jiang region of china]. | there are more than 1000 salt lakes situated in northern and western regions of china and 790 of these salt lakes are in xinjiang uigur autonomous region and qinghai, tibet, inner mongolia. to better understand halophilic archaeal diversity of salt lakes in xinjiang, water and sediment samples were collected from two salt lakes, namely aibi salt lake and aiding salt lake, and the halophilic archaeal diversity of these samples was determined. totally eighty-six halophilic archeal strains, of whic ... | 2006 | 16736571 |
| phylogenetic relationships within the order halobacteriales inferred from 16s rrna gene sequences. | phylogenetic relationships within the halophilic archaea were inferred from comparisons of the 16s rrna gene sequences from 61 strains, representing 18 genera with validly published names within the order halobacteriales. trees produced using distance-matrix (least-squares and neighbour-joining) methods affirm with strong bootstrap support that the members of the order halobacteriales are a monophyletic group. ten genera were supported as monophyletic groups [haloarcula, halobiforma, halococcus, ... | 2006 | 16738095 |
| natronorubrum aibiense sp. nov., an extremely halophilic archaeon isolated from aibi salt lake in xin-jiang, china, and emended description of the genus natronorubrum. | an extremely halophilic archaeon (strain 7-3(t)) was isolated from aibi salt lake in xin-jiang, china. it formed small (<1.0 mm), red, elevated colonies on salt-milk agar medium. strain 7-3(t) was able to grow at ph 6.5-9.5 (optimum ph 8.0) and at 20-50 degrees c (optimum 45 degrees c). analysis of its 16s rrna gene sequence indicated that strain 7-3(t) was phylogenetically related to members of the genus natronorubrum, with sequence similarities of 97.0 % to natronorubrum bangense a33(t) and 95 ... | 2006 | 16825622 |
| [studies on bacteriorhodopsin gene and sequence of 16s rrna encoding genes of halophilic archaea of xingjiang aibi lake]. | one hundred and forty-eight strains of halophilic archaea were isolated from 40 samples of soil, lake water, and silt. to study and analyze the species and bacteriorhodopsin(br) protein resource, partial dna fragments encoding br protein from helix c to helix g andl6s rrna encoding genes from 6 strains of halophilic archaea were amplified by polymerase chain(pcr) , and their dna sequences were determined. the results indicate that the reduced amino acid sequences of br protein from helix c to he ... | 2007 | 17366887 |
| natronorubrum sulfidifaciens sp. nov., an extremely haloalkaliphilic archaeon isolated from aiding salt lake in xin-jiang, china. | an extremely haloalkaliphilic archaeon, strain ad2(t), was isolated from aiding salt lake in xin-jiang, china. strain ad2(t) required at least 12 % nacl for growth. mgcl(2) was not required. the isolate was able to grow over a ph range of 8.0-10.0 and temperature range of 20-55 degrees c, with optimal growth at ph 8.7-9.2 and 44-47 degrees c. the major polar lipids of strain ad2(t) were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester; glycolipids were not detected. analysis ... | 2007 | 17392197 |
| phylogenetic relationships within the family halobacteriaceae inferred from rpob' gene and protein sequences. | in order to clarify the current phylogeny of the haloarchaea, particularly the closely related genera that have been difficult to sort out using 16s rrna gene sequences, the dna-dependent rna polymerase subunit b' gene (rpob') was used as a complementary molecular marker. partial sequences of the gene were determined from 16 strains of the family halobacteriaceae. comparisons of phylogenetic trees inferred from the gene and protein sequences as well as from corresponding 16s rrna gene sequences ... | 2007 | 17911299 |
| emended descriptions of genera of the family halobacteriaceae. | the family halobacteriaceae currently contains 96 species whose names have been validly published, classified in 27 genera (as of september 2008). in recent years, many novel species have been added to the established genera but, in many cases, one or more properties of the novel species do not agree with the published descriptions of the genera. authors have often failed to provide emended genus descriptions when necessary. following discussions of the international committee on systematics of ... | 2009 | 19244452 |
| natronorubrum sediminis sp. nov., an archaeon isolated from a saline lake. | two novel haloalkaliphilic archaea, strains cg-6t and cg-4, were isolated from sediment of the hypersaline lake chagannor in inner mongolia, china. cells of the two strains were pleomorphic, non-motile and strictly aerobic. they required at least 2.5 m nacl for growth, with optimum growth at 3.4 m nacl. they grew at ph 8.0-11.0, with optimum growth at ph 9.0. hypotonic treatment with less than 1.5 m nacl caused cell lysis. the two strains had similar polar lipid compositions, possessing c20c20 a ... | 2010 | 19767366 |
| further refinement of the phylogeny of the halobacteriaceae based on the full-length rna polymerase subunit b' (rpob') gene. | a considerable number of species of the halobacteriaceae possess multiple copies of the 16s rrna gene that exhibit more than 5 % divergence, complicating phylogenetic interpretations. two additional problems have been pointed out: (i) the genera haloterrigena and natrinema show a very close relationship, with some species being shown to overlap in phylogenetic trees reconstructed by the neighbour-joining method, and (ii) alkaliphilic and neutrophilic species of the genus natrialba form definitel ... | 2010 | 19946058 |