| a novel pmoa lineage represented by the acidophilic methanotrophic bacterium methylocapsa acidiphila [correction of acidophila] b2. | a fragment of the functional gene pmoa, which encodes the active-site polypeptide of particulate methane monooxygenase (pmmo), was pcr-amplified from dna of the recently described acidophilic methanotrophic bacterium methylocapsa acidiphila [corrected] b2. this methanotroph was isolated from an acidic sphagnum peat bog and possesses a novel type iii arrangement of intracytoplasmic membranes. comparative sequence analysis revealed that the inferred peptide sequence of pmoa of methylocapsa acidiph ... | 2001 | 11797053 |
| methylocapsa acidiphila gen. nov., sp. nov., a novel methane-oxidizing and dinitrogen-fixing acidophilic bacterium from sphagnum bog. | a novel genus and species, methylocapsa acidiphila gen. nov., sp. nov., are proposed for a methane-oxidizing bacterium isolated from an acidic sphagnum peat bog. this bacterium, designated strain b2t, represents aerobic, gram-negative, colourless, non-motile, curved coccoids that form conglomerates covered by an extracellular polysaccharide matrix. the cells use methane and methanol as sole sources of carbon and energy and utilize the serine pathway for carbon assimilation. strain b2t is a moder ... | 2002 | 11837310 |
| methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol. | two strains of gram-negative, aerobic, non-pigmented, non-motile, rod-shaped, methane-oxidizing bacteria were isolated from an acidic forest cambisol near marburg, germany, and were designated as strains bl2(t) and a1. these bacteria were morphologically and phenotypically similar to methylocella palustris k(t). the cells possess a highly specific bipolar appearance. they lack the intracytoplasmic membranes common to all methane-oxidizing bacteria except methylocella, but contain a vesicular mem ... | 2003 | 13130000 |
| nifh and nifd phylogenies: an evolutionary basis for understanding nitrogen fixation capabilities of methanotrophic bacteria. | the ability to utilize dinitrogen as a nitrogen source is an important phenotypic trait in most currently known methanotrophic bacteria (mb). this trait is especially important for acidophilic mb, which inhabit acidic oligotrophic environments, highly depleted in available nitrogen compounds. phylogenetically, acidophilic mb are most closely related to heterotrophic dinitrogen-fixing bacteria of the genus beijerinckia: to further explore the phylogenetic linkage between these metabolically diffe ... | 2004 | 15133093 |
| [inhibition of the growth and methane consumption in methylocapsa acidiphila by mineral salts]. | | 2004 | 15521186 |
| first genome data from uncultured upland soil cluster alpha methanotrophs provide further evidence for a close phylogenetic relationship to methylocapsa acidiphila b2 and for high-affinity methanotrophy involving particulate methane monooxygenase. | members of upland soil cluster alpha (usc alpha) are assumed to be methanotrophic bacteria (mb) adapted to the trace level of atmospheric methane. so far, these mb have eluded all cultivation attempts. while the 16s rrna phylogeny of usc alpha members is still not known, phylogenies constructed for the active-site polypeptide (encoded by pmoa) of particulate methane monooxygenase (pmmo) placed usc alpha next to the alphaproteobacterial methylocapsa acidiphila b2. to assess whether the pmoa tree ... | 2005 | 16269789 |
| aerobic methanotrophic bacteria of cold ecosystems. | this review summarizes the recent advances in understanding the ecophysiological role and structure-function features of methanotrophic bacteria living in various cold ecosystems. the occurrence of methanotrophs in a majority of psychrosphere sites was verified by direct measurement of their methane-utilizing activity, by electron microscopy and immunofluorescent observations, and analyses of specific signatures in cellular phospholipids and total dnas extracted from environmental samples. surpr ... | 2005 | 16329925 |
| methylovirgula ligni gen. nov., sp. nov., an obligately acidophilic, facultatively methylotrophic bacterium with a highly divergent mxaf gene. | two strains of gram-negative, aerobic, non-pigmented, non-motile, rod-shaped bacteria were isolated from beechwood blocks during decay by the white-rot fungus hypholoma fasciculare and were designated strains bw863(t) and bw872. they are capable of methylotrophic growth and assimilate carbon via the ribulose-bisphosphate pathway. in addition to methanol, the novel isolates utilized ethanol, pyruvate and malate. strains bw863(t) and bw872 are obligately acidophilic, mesophilic organisms capable o ... | 2009 | 19622650 |
| differential detection of type ii methanotrophic bacteria in acidic peatlands using newly developed 16s rrna-targeted fluorescent oligonucleotide probes. | abstract based on an extensive 16s rrna sequence database for type ii methanotrophic bacteria, a set of 16s rrna-targeted oligonucleotide probes was developed for differential detection of specific phylogenetic groups of these bacteria by fluorescence in situ hybridisation (fish). this set of oligonucleotides included a genus-specific probe for methylocystis (mcyst-1432) and three species-specific probes for methylosinus sporium (msins-647), methylosinus trichosporium (msint-1268) and the recent ... | 2003 | 19719661 |
| methylocapsa aurea sp. nov., a facultative methanotroph possessing a particulate methane monooxygenase, and emended description of the genus methylocapsa. | an aerobic, methanotrophic bacterium, designated kyg(t), was isolated from a forest soil in germany. cells of strain kyg(t) were gram-negative, non-motile, slightly curved rods that multiplied by binary fission and produced yellow colonies. the cells contained intracellular granules of poly-β-hydroxybutyrate at each cell pole, a particulate methane monooxygenase (pmmo) and stacks of intracytoplasmic membranes (icms) packed in parallel along one side of the cell envelope. strain kyg(t) grew at ph ... | 2010 | 20061505 |
| the (d)evolution of methanotrophy in the beijerinckiaceae--a comparative genomics analysis. | the alphaproteobacterial family beijerinckiaceae contains generalists that grow on a wide range of substrates, and specialists that grow only on methane and methanol. we investigated the evolution of this family by comparing the genomes of the generalist organotroph beijerinckia indica, the facultative methanotroph methylocella silvestris and the obligate methanotroph methylocapsa acidiphila. highly resolved phylogenetic construction based on universally conserved genes demonstrated that the bei ... | 2013 | 23985741 |