| a new brazilian passiflora leafminer: spinivalva gaucha, gen. n., sp. n. (lepidoptera, gracillariidae, gracillariinae), the first gracillariid without a sap-feeding instar. | male, female, pupa, larva and egg of a new genus and species of gracillariidae (gracillariinae), spinivalva gaucha moreira and vargas from southern brazil are described and illustrated with the aid of optical and scanning electron microscopy. a preliminary analysis of mitochondrial dna sequences including members of related lineages is also provided. the immature stages are associated with passiflora actinia, passiflora misera and passiflora suberosa (passifloraceae), and build mines on the adax ... | 2013 | 23794860 |
| haemoglobin-enhanced mitotic division in cultured protoplasts. | protoplasts from cell suspensions of albino petunia hybrida cv. comanche were cultured for 9 days in nutrient medium containing erythrogen, a purified bovine haemoglobin solution (supplied at 10% w/v) at 1:50-1:500 (v/v). in some assessments, the non-ionic surfactant pluronic f-68 (poloxamer 188), was also added to the culture medium at 0.01-1.0% (w/v). erythrogen at 1:50 (v/v) increased the mean initial protoplast plating efficiency (ipe; 18.5 +/- 0.8%, n = 5 throughout) by 64% (p < 0.001) abov ... | 1997 | 9352062 |