| an overexpression of chalcone reductase of pueraria montana var. lobata alters biosynthesis of anthocyanin and 5'-deoxyflavonoids in transgenic tobacco. | we isolated the chalcone reductase (pl-chr) gene of pueraria montana var. lobata by using a pcr strategy from cdna pools of storage roots. a high level of expression of rna was found in both stems and roots. the genomic southern blot result suggests that pl-chr exists as a member of a small gene family. by introducing a pl-chr gene under the control of the 35s camv promoter into the pink-flowering xanthi line of nicotiana tabacum, the flower color was changed from pink to white-to-pink. the cont ... | 2003 | 12646206 |
| evolution of the isoprene biosynthetic pathway in kudzu. | isoprene synthase converts dimethylallyl diphosphate, derived from the methylerythritol 4-phosphate (mep) pathway, to isoprene. isoprene is made by some plants in substantial amounts, which affects atmospheric chemistry, while other plants make no isoprene. as part of our long-term study of isoprene synthesis, the genetics of the isoprene biosynthetic pathway of the isoprene emitter, kudzu (pueraria montana), was compared with similar genes in arabidopsis (arabidopsis thaliana), which does not m ... | 2005 | 15653811 |
| biology and preliminary host range assessment of two potential kudzu biological control agents. | two insect species from china, gonioctena tredecimmaculata (jacoby) (coleoptera: chrysomelidae) and ornatalcides (mesalcidodes) trifidus (pascoe) (coleoptera: curculionidae), were studied in quarantine in the united states as potential biological control agents for kudzu, pueraria montana variety lobata (willd.) maesen and s. almeida. adults of g. tredecimmaculata were ovoviviparous and reproduced throughout the summer, producing offspring that had an obligate adult diapause. in no-choice tests, ... | 2007 | 18284771 |
| engineering a platform for photosynthetic isoprene production in cyanobacteria, using synechocystis as the model organism. | the concept of "photosynthetic biofuels" envisions application of a single organism, acting both as photo-catalyst and producer of ready-made fuel. this concept was applied upon genetic engineering of the cyanobacterium synechocystis, conferring the ability to generate volatile isoprene hydrocarbons from co(2) and h(2)o. heterologous expression of the pueraria montana (kudzu) isoprene synthase (isps) gene in synechocystis enabled photosynthetic isoprene generation in these cyanobacteria. codon-u ... | 2010 | 19833224 |
| tracking deposition of a 14c-radiolabeled kudzu hairy root-derived isoflavone-rich fraction into bone. | hairy roots were induced in four genotypes from three kudzu species (pueraria montana var. lobata, p. lobata and p. phaseoloides) in vitro using agrobacterium rhizogenes to stimulate rapid secondary metabolite synthesis. hairy roots from p. montana var. lobata (united states department of agriculture no. pi 434246) yielded the highest puerarin and total isoflavone content and the greatest new biomass per growth cycle among the genotypes evaluated. hairy roots from this genotype were selected for ... | 2010 | 20851832 |
| diffusion-based process for carbon dioxide uptake and isoprene emission in gaseous/aqueous two-phase photobioreactors by photosynthetic microorganisms. | photosynthesis for the generation of fuels and chemicals from cyanobacteria and microalgae offers the promise of a single host organism acting both as photocatalyst and processor, performing sunlight absorption and utilization, as well as co(2) assimilation and conversion into product. however, there is a need to develop methods for generating, sequestering and trapping such bio-products in an efficient and cost-effective manner that is suitable for industrial scale-up and exploitation. a sealed ... | 2011 | 21830206 |
| estrogenic plant extracts reverse weight gain and fat accumulation without causing mammary gland or uterine proliferation. | long-term estrogen deficiency increases the risk of obesity, diabetes and metabolic syndrome in postmenopausal women. menopausal hormone therapy containing estrogens might prevent these conditions, but its prolonged use increases the risk of breast cancer, as wells as endometrial cancer if used without progestins. animal studies indicate that beneficial effects of estrogens in adipose tissue and adverse effects on mammary gland and uterus are mediated by estrogen receptor alpha (erĪ±). one strate ... | 2011 | 22163294 |
| identification of novel isoprene synthases through genome mining and expression in escherichia coli. | isoprene is a naturally produced hydrocarbon emitted into the atmosphere by green plants. it is also a constituent of synthetic rubber and a potential biofuel. microbial production of isoprene can become a sustainable alternative to the prevailing chemical production of isoprene from petroleum. in this work, sequence homology searches were conducted to find novel isoprene synthases. candidate sequences were functionally expressed in escherichia coli and the desired enzymes were identified based ... | 2015 | 26275749 |
| macrocyclic trichothecenes are undetectable in kudzu (pueraria montana) plants treated with a high-producing isolate of myrothecium verrucaria. | myrothecium verrucaria was found to be an effective pathogen against kudzu grown in the greenhouse and the field. m. verrucaria produced large amounts of macrocyclic trichothecenes when cultured on solid rice medium, including epiroridin e (16.8 mg/g crude extract), epiisororidin e (1 mg/g), roridin e (8.7 mg/g), roridin h (31.3 mg/g), trichoverrin a (0.6 mg/g), trichoverrin b (0.1 mg/g), verrucarin a (37.4 mg/g), and verrucarin j (2.2 mg/g). most of these toxins were also isolated from m. verru ... | 2001 | 11551550 |
| isoprene hydrocarbons production upon heterologous transformation of saccharomyces cerevisiae. | isoprene (2-methyl-1,3-butadiene; c(5) h(8) ) is naturally produced by photosynthesis and emitted in the atmosphere by the leaves of many herbaceous, deciduous and woody plants. fermentative yeast and fungi (ascomycota) are not genetically endowed with the isoprene production process. the work investigated whether ascomycota can be genetically modified and endowed with the property of constitutive isoprene production. | 2012 | 22519412 |
| nitric oxide and nitrous oxide production by soybean and winged bean during the in vivo nitrate reductase assay. | this study was conducted to determine by gas chromatography (gc) and mass spectrometry (ms) the identity and the quantity of volatile n products produced during the helium-purged in vivo nr assay of soybean (glycine max [l.] merr. cv williams) and winged bean (psophocarpus tetragonolobus [l.] dc. cv lunita) leaflets. gaseous material for identification and quantitation was collected by cryogenic trapping of volatile compounds carried in the he-purge gas stream. as opposed to an earlier report, a ... | 1986 | 16665099 |
| seasonal population dynamics of megacopta cribraria (hemiptera: plataspidae) in kudzu and soybean, and implication for insecticidal management in soybean. | megacopta cribraria (f.), an invasive species introduced from asia in 2009, is now prolific in the southeastern united states. megacopta cribraria develops primarily on kudzu and soybean completing two generations. it is not well understood how this economic pest is affected by changes in geographic distribution in the united states or how population levels have changed since its establishment. the effect of insecticide application timing on field populations of m. cribraria is not well document ... | 2017 | 28039424 |
| megacopta cribraria (hemiptera: plataspidae) population dynamics in soybeans as influenced by planting date, maturity group, and insecticide use. | since its unintentional introduction during 2009, megacopta cribraria (f.) has spread rapidly throughout the southeastern united states, mainly feeding and reproducing on kudzu, pueraria montana loureiro (merr.) variety lobata (willdenow), and soybeans, glycine max (l.) merr. megacopta cribraria has become a serious economic pest in soybeans, forcing growers to rely solely on insecticide applications to control this insect. the main objective of this study was to investigate if variation in plan ... | 2016 | 26896533 |
| the biology and preliminary host range of megacopta cribraria (heteroptera: plataspidae) and its impact on kudzu growth. | the bean plataspid, megacopta cribraria (f.), recently was discovered in the united states feeding on kudzu, pueraria montana lour. (merr.) variety lobata (willd.), an economically important invasive vine. we studied its biology on kudzu and its impact on kudzu growth. we also tested its ability to use other common forest legumes for oviposition and development. flight intercept traps operated from 17 may 2010 to 31 may 2011 in a kudzu field near athens, ga showed three peaks of adult flight act ... | 2012 | 22525058 |
| population genetic baseline of the first plataspid stink bug symbiosis (hemiptera: heteroptera: plataspidae) reported in north america. | the stink bug, megacopta cribraria, has an obligate relationship with a bacterial endosymbiont which allows it to feed on legumes. the insect is a pest of soybeans in asia and was first reported in the western hemisphere in october 2009 on kudzu vine, pueraria montana, in north georgia, usa. by october 2010 m. cribraria had been confirmed in 80 counties in georgia actively feeding on kudzu vine and soybean plants. since the symbiosis may support the bug's ecological expansions, a population gene ... | 2011 | 26467727 |
| modeling landscape-scale pathogen spillover between domesticated and wild hosts: asian soybean rust and kudzu. | many emerging pathogens infect both domesticated and wild host species, creating the potential for pathogen transmission between domesticated and wild populations. this common situation raises the question of whether managing negative impacts of disease on a focal host population (whether domesticated, endangered, or pest) requires management of only the domesticated host, only the wild host, or both. to evaluate the roles of domesticated and wild hosts in the dynamics of shared pathogens, we de ... | 2010 | 20405808 |