| crystal structure of turnip yellows virus protein. | | 1956 | 13324103 |
| genetic analyses of the host-pathogen system turnip yellows virus (tuyv)-rapeseed (brassica napus l.) and development of molecular markers for tuyv-resistance. | the aphid transmitted turnip yellows virus (tuyv) has become a serious pathogen in many rapeseed (brassica napus l.) growing areas. three-years' field trials were carried out to get detailed information on the genetics of tuyv resistance derived from the resynthesised b. napus line 'r54' and to develop closely linked markers. f(1) plants and segregating doubled-haploid (dh) populations derived from crosses to susceptible cultivars were analysed using artificial inoculation with virus-bearing aph ... | 2010 | 19904523 |
| a reinvestigation provides no evidence for sugar residues on structural proteins of poleroviruses and argues against a role for glycosylation of virus structural proteins in aphid transmission. | poleroviruses are strictly transmitted by aphids. glycosylation of turnip yellows virus (tuyv) was previously reported and this modification was supposed to be required for aphid transmission. using different approaches based on (i) a lectin-binding assay, (ii) use of specific complex glycan antibodies and (iii) mass spectrometry, we found no evidence that the structural proteins of tuyv and cucurbit aphid-borne yellow virus (cabyv) carry glycan residues. moreover, mutation of each of the four p ... | 2010 | 20416918 |
| beet poleroviruses: close friends or distant relatives? | summary taxonomy: there are three members of the genus polerovirus (family luteoviridae) that induce yellowing of sugar beet: beet mild yellowing virus (bmyv), beet chlorosis virus (bchv) and beet western yellows virus-usa (bwyv-usa, fig. 1). non-beet-infecting isolates of bwyv found particularly within europe have now been re-named turnip yellows virus (tuyv). species-specific antibodies are unavailable, but the viruses can be distinguished by rt-pcr using primers specifically designed to the 5 ... | 2005 | 20565633 |
| detection and discrimination of members of the family luteoviridae by real-time pcr and sybr® greenerâ„¢ melting curve analysis. | this study investigated the suitability of a two step real-time rt-pcr melting curve analysis as a tool for the detection and discrimination of nine species in the plant virus family luteoviridae, being soybean dwarf virus [sbdv], bean leafroll virus [blrv], beet chlorosis virus [bchv], beet mild yellowing virus [bmyv], beet western yellows virus [bwyv], cereal yellow dwarf virus-rpv [cydv-rpv], cucurbit aphid-borne yellows virus [cabyv], potato leafroll virus [plrv] and turnip yellows virus [tu ... | 2010 | 20933015 |
| Molecular characterization of two genotypes of a new polerovirus infecting brassicas in China. | The genomic RNA sequences of two genotypes of a brassica-infecting polerovirus from China were determined. Sequence analysis revealed that the virus was closely related to but significantly different from turnip yellows virus (TuYV). This virus and other poleroviruses, including TuYV, had less than 90% amino acid sequence identity in all gene products except the coat protein. Based on the molecular criterion (>10% amino acid sequence difference) for species demarcation in the genus Polerovirus, ... | 2011 | 21874520 |
| exotic and indigenous viruses infect wild populations and captive collections of temperate terrestrial orchids (diuris species) in australia. | four species of diuris temperate terrestrial orchids from wild and captive populations were tested for the presence of polyadenylated rna viruses. the genomes of three exotic viruses were determined: two potyviruses, bean yellow mosaic virus and ornithogalum mosaic virus, and the polerovirus turnip yellows virus. the genomes of five indigenous viruses were detected, including four novel species. they were the potyvirus blue squill virus a, another potyvirus, two proposed capilloviruses, and a pa ... | 2013 | 23089850 |
| genome sequence of a recombinant brassica yellows virus infecting chinese cabbage. | rna from a chinese cabbage plant (brassica campestris ssp. pekinensis) showing leaf malformation and mottling was labeled and hybridized to a dna chip capable of detecting plant viruses and viroids. probes specific for beet mild yellowing virus (bmyv) and beet western yellows virus (bwyv) yielded positive results, suggesting that the plant was infected by a polerovirus. primers designed from the sequences of the positive probes were used to amplify and sequence one portion of the viral genome. t ... | 2015 | 25352211 |
| generic detection of poleroviruses using an rt-pcr assay targeting the rdrp coding sequence. | in this study a two-step rt-pcr assay was developed for the generic detection of poleroviruses. the rdrp coding region was selected as the primers' target, since it differs significantly from that of other members in the family luteoviridae and its sequence can be more informative than other regions in the viral genome. species specific rt-pcr assays targeting the same region were also developed for the detection of the six most widespread poleroviral species (beet mild yellowing virus, beet wes ... | 2014 | 24374125 |
| closely related poleroviruses depend on distinct translation initiation factors to infect arabidopsis thaliana. | in addition to being essential for translation of eukaryotic mrna, translation initiation factors are also key components of plant-virus interactions. in order to address the involvement of these factors in the infectious cycle of poleroviruses (aphid-transmitted, phloem-limited viruses), the accumulation of three poleroviruses was followed in arabidopsis thaliana mutant lines impaired in the synthesis of translation initiation factors in the eif4e and eif4g families. we found that efficient acc ... | 2013 | 23013438 |
| elicitation of hypersensitive responses in nicotiana glutinosa by the suppressor of rna silencing protein p0 from poleroviruses. | plant disease resistance (r) proteins that confer resistance to viruses recognize viral gene products with diverse functions, including viral suppressors of rna silencing (vsrs). the p0 protein from poleroviruses is a vsr that targets the argonaute1 (ago1) protein for degradation, thereby disrupting rna silencing and antiviral defences. here, we report resistance against poleroviruses in nicotiana glutinosa directed against turnip yellows virus (tuyv) and potato leafroll virus (plrv). the p0 pro ... | 2015 | 25187258 |
| a protein kinase binds the c-terminal domain of the readthrough protein of turnip yellows virus and regulates virus accumulation. | turnip yellows virus (tuyv), a phloem-limited virus, encodes a 74kda protein known as the readthrough protein (rt) involved in virus movement. we show here that a tuyv mutant deleted of the c-terminal part of the rt protein (tuyv-∆rtcter) was affected in long-distance trafficking in a host-specific manner. by using the c-terminal domain of the rt protein as a bait in a yeast two-hybrid screen of a phloem cdna library from arabidopsis thaliana we identified the calcineurin b-like protein-interact ... | 2015 | 26402374 |
| impact of disease on diversity and productivity of plant populations. | experiments were conducted on the role of intra- and inter-genotypic competition in ecological processes operating at the population scale in diseased plant populations.combinations of arabidopsis thaliana genotypes showing variation for phenotypic traits relating to competitive ability and pathogen compatibility were infected with the oomycete hyaloperonospora arabidopsidis and turnip yellows virus in separate experiments. plant fitness and competitive ability were estimated from phenotypic mea ... | 2016 | 27546948 |
| discovery of a small non-aug-initiated orf in poleroviruses and luteoviruses that is required for long-distance movement. | viruses in the family luteoviridae have positive-sense rna genomes of around 5.2 to 6.3 kb, and they are limited to the phloem in infected plants. the luteovirus and polerovirus genera include all but one virus in the luteoviridae. they share a common gene block, which encodes the coat protein (orf3), a movement protein (orf4), and a carboxy-terminal extension to the coat protein (orf5). these three proteins all have been reported to participate in the phloem-specific movement of the virus in pl ... | 2015 | 25946037 |
| formation of virions is strictly required for turnip yellows virus long-distance movement in plants. | viral genomic rna of the turnip yellows virus (tuyv; genus polerovirus; family luteoviridae) is protected in virions formed by the major capsid protein (cp) and the minor component, the readthrough (rt*) protein. long-distance transport, used commonly by viruses to systemically infect host plants, occurs in phloem sieve elements and two viral forms of transport have been described: virions and ribonucleoprotein (rnp) complexes. with regard to poleroviruses, virions have always been presumed to b ... | 2014 | 24214396 |
| high throughput phenotyping for aphid resistance in large plant collections. | phloem-feeding insects are among the most devastating pests worldwide. they not only cause damage by feeding from the phloem, thereby depleting the plant from photo-assimilates, but also by vectoring viruses. until now, the main way to prevent such problems is the frequent use of insecticides. applying resistant varieties would be a more environmental friendly and sustainable solution. for this, resistant sources need to be identified first. up to now there were no methods suitable for high thro ... | 2012 | 22901796 |
| plant infection by two different viruses induce contrasting changes of vectors fitness and behavior. | insect-vectored plant viruses can induce changes in plant phenotypes, thus influencing plant-vector interactions in a way that may promote their dispersal according to their mode of transmission (i.e., circulative vs. noncirculative). this indirect vector manipulation requires host-virus-vector coevolution and would thus be effective solely in very specific plant-virus-vector species associations. some studies suggest this manipulation may depend on multiple factors relative to various intrinsic ... | 2017 | 28731285 |
| insights in luteovirid structural biology guided by chemical cross-linking and high resolution mass spectrometry. | interactions among plant pathogenic viruses in the family luteoviridae and their plant hosts and insect vectors are governed by the topology of the viral capsid, which is the sole vehicle for long distance movement of the viral genome. previous application of a mass spectrometry-compatible cross-linker to preparations of the luteovirid potato leafroll virus (plrv; luteoviridae: polerovirus) revealed a detailed network of interactions between viral structural proteins and enabled generation of th ... | 2017 | 28502641 |
| drought reduces transmission of turnip yellows virus, an insect-vectored circulative virus. | application of a severe water deficit to arabidopsis thaliana plants infected with a mutant of turnip yellows virus (tuyv, family luteoviridae) triggers a significant alteration of several plant phenology traits and strongly reduces the transmission efficiency of the virus by aphids. although virus accumulation in water-stressed plants was similar to that in plants grown under well-watered conditions, virus accumulation was reduced in aphids fed on plants under water deficit. these results sugge ... | 2017 | 28756104 |
| systemic propagation of a fluorescent infectious clone of a polerovirus following inoculation by agrobacteria and aphids. | a fluorescent viral clone of the polerovirus turnip yellows virus (tuyv) was engineered by introducing the enhanced green fluorescent protein (egfp) sequence into the non-structural domain sequence of the readthrough protein, a minor capsid protein. the resulting recombinant virus, referred to as tuyv-rtgfp, was infectious in several plant species when delivered by agroinoculation and invaded efficiently non-inoculated leaves. as expected for poleroviruses, which infect only phloem cells, the fl ... | 2017 | 28661469 |