| improved detection and differentiation of poleroviruses infecting beet or rape by multiplex rt-pcr. | three distinct species of virus inducing yellowing of beet, beet mild yellowing virus (bmyv), brassica yellows virus (bryv, synonym bwyv) and beet chlorosis virus (bchv) have been characterised from the genus polerovirus. until recently, no available tools were available to allow accurate and reliable distinction of the three species. based on previous nucleotide sequence alignments and phylogenetic studies, we show that the use of molecular methods enabled the discrimination of these three beet ... | 2000 | 10996635 |
| biological properties and molecular characterization of beet chlorosis virus (bchv). | two distinct viruses belonging to the polerovirus genus, in the family luteoviridae, have been described as being able to induce mild yellowing on sugar beet: beet mild yellowing virus (bmyv) and more recently, beet chlorosis virus (bchv). we have analysed biological properties and molecular organisation of two strains of bchv, one collected in england and the second from california. the biological data suggested that bchv displayed a narrower host range compared to bmyv and beet western yellows ... | 2002 | 12038685 |
| decision making in controlling virus yellows of sugar beet in the uk. | virus yellows is an important disease affecting yield in sugar beet in the uk. myzus persicae (sulzer) is the most effective and efficient aphid vector of the three viruses causing the disease: beet yellows virus, beet mild yellowing virus and beet chlorosis virus. control of virus yellows disease is thus focused on the study and control of this aphid species. uk national surveys of virus yellows began in 1946 and these data helped to formulate disease forecasting schemes to optimise control. ov ... | 2004 | 15260306 |
| development of a multiplex immunocapture-rt-pcr for simultaneous detection of bmyv and bchv in plants and single aphids. | a multiplex immunocapture-reverse transcription-polymerase chain reaction protocol (mic-rt-pcr) was successfully developed to improve the detection of beet mild yellowing virus (bmyv) and beet chlorosis virus (bchv) in plants and aphids in single and mixed infections. viral particles from plant and aphid extracts were enriched by antibody-capture and lysed by heating to release the viral rna. during the rt-pcr step, 5' end sequences specific to each virus were amplified and the products analysed ... | 2007 | 17686535 |
| biological, serological, and molecular variability suggest three distinct polerovirus species infecting beet or rape. | yellowing diseases of sugar beet can be caused by a range of strains classified as beet mild yellowing virus (bmyv) or beet western yellows virus (bwyv), both belonging to the genus polerovirus of the family luteoviridae. host range, genomic, and serological studies have shown that isolates of these viruses can be grouped into three distinct species. within these species, the coat protein amino acid sequences are highly conserved (more than 90% homology), whereas the p0 sequences (open reading f ... | 2000 | 18944550 |
| p0 proteins of european beet-infecting poleroviruses display variable rna silencing suppression activity. | post-transcriptional gene silencing (ptgs), or rna silencing, is one of the key mechanisms of antiviral defence used by plants. to counter this defence response, viruses produce suppressor proteins that are able to inhibit the ptgs pathway or to interfere with some of its function. the aim of this study was to evaluate the rna silencing suppressor (rss) activity of p0 proteins from selected european isolates of the beet-infecting poleroviruses beet chlorosis virus (bchv) and beet mild yellowing ... | 2010 | 19955562 |
| beet poleroviruses: close friends or distant relatives? | summary taxonomy: there are three members of the genus polerovirus (family luteoviridae) that induce yellowing of sugar beet: beet mild yellowing virus (bmyv), beet chlorosis virus (bchv) and beet western yellows virus-usa (bwyv-usa, fig. 1). non-beet-infecting isolates of bwyv found particularly within europe have now been re-named turnip yellows virus (tuyv). species-specific antibodies are unavailable, but the viruses can be distinguished by rt-pcr using primers specifically designed to the 5 ... | 2005 | 20565633 |
| detection and discrimination of members of the family luteoviridae by real-time pcr and sybr® greener™ melting curve analysis. | this study investigated the suitability of a two step real-time rt-pcr melting curve analysis as a tool for the detection and discrimination of nine species in the plant virus family luteoviridae, being soybean dwarf virus [sbdv], bean leafroll virus [blrv], beet chlorosis virus [bchv], beet mild yellowing virus [bmyv], beet western yellows virus [bwyv], cereal yellow dwarf virus-rpv [cydv-rpv], cucurbit aphid-borne yellows virus [cabyv], potato leafroll virus [plrv] and turnip yellows virus [tu ... | 2010 | 20933015 |
| genome sequence of a recombinant brassica yellows virus infecting chinese cabbage. | rna from a chinese cabbage plant (brassica campestris ssp. pekinensis) showing leaf malformation and mottling was labeled and hybridized to a dna chip capable of detecting plant viruses and viroids. probes specific for beet mild yellowing virus (bmyv) and beet western yellows virus (bwyv) yielded positive results, suggesting that the plant was infected by a polerovirus. primers designed from the sequences of the positive probes were used to amplify and sequence one portion of the viral genome. t ... | 2015 | 25352211 |
| phylogenetic relationships and the occurrence of interspecific recombination between beet chlorosis virus (bchv) and beet mild yellowing virus (bmyv). | samples containing two viruses belonging to the genus polerovirus, beet chlorosis virus (bchv) and beet mild yellowing virus (bmyv), were collected from french and polish sugar beet fields. the molecular properties of 24 isolates of bchv and bmyv were investigated, and their genetic diversity was examined in the coat protein (cp)- and p0-encoding genes. for the first time, we have demonstrated that beet polerovirus populations include recombinants between bchv and bmyv containing breakpoints wit ... | 2015 | 25283610 |