| use of in vivo 13c nuclear magnetic resonance spectroscopy to elucidate l-arabinose metabolism in yeasts. | candida arabinofermentans pycc 5603(t) and pichia guilliermondii pycc 3012 were shown to grow well on l-arabinose, albeit exhibiting distinct features that justify an in-depth comparative study of their respective pentose catabolism. carbon-13 labeling experiments coupled with in vivo nuclear magnetic resonance (nmr) spectroscopy were used to investigate l-arabinose metabolism in these yeasts, thereby complementing recently reported physiological and enzymatic data. the label supplied in l-[2-(1 ... | 2008 | 18245253 |
| l-arabinose transport and catabolism in yeast. | two yeasts, candida arabinofermentans pycc 5603(t) and pichia guilliermondii pycc 3012, which show rapid growth on l-arabinose and very high rates of l-arabinose uptake on screening, were selected for characterization of l-arabinose transport and the first steps of intracellular l-arabinose metabolism. the kinetics of l-arabinose uptake revealed at least two transport systems with distinct substrate affinities, specificities, functional mechanisms and regulatory properties. the l-arabinose catab ... | 2007 | 17627668 |
| l-arabinose metabolism in candida arabinofermentans pycc 5603t and pichia guilliermondii pycc 3012: influence of sugar and oxygen on product formation. | l-arabinose utilization by the yeasts candida arabinofermentans pycc 5603(t) and pichia guilliermondii pycc 3012 was investigated in aerobic batch cultures and compared, under similar conditions, to d-glucose and d-xylose metabolism. at high aeration levels, only biomass was formed from all the three sugars. when oxygen became limited, ethanol was produced from d-glucose, demonstrating a fermentative pathway in these yeasts. however, pentoses were essentially respired and, under oxygen limitatio ... | 2007 | 17262211 |
| candida arabinofermentans, a new l-arabinose fermenting yeast. | candida arabinofermentans (type strain nrrl yb-2248, cbs 8468), a new yeast that ferments the pentose l-arabinose, is described. the three known strains of this new species were isolated from insect frass of pine and larch trees in the u.s. phylogenetic analysis of nucleotide sequences from the d1/d2 domain of large subunit (26s) ribosomal dna places c. arabinofermentans among the methanol-assimilating yeasts and most closely related to candida ovalis. strains of the new species produce 0.7-1.9 ... | 1998 | 10081583 |