| prevalence of coccidia in pigs in zimbabwe. | faecal specimens from 162 piglets, aged 1-5 months, from four farms around harare were examined for coccidia during 1989-1990. oocyst count per gram (opg) of faeces was determined by the mcmaster technique. identification of eimeria species was done after sporulation of oocysts in 2.5% potassium dichromate solution. measurements of sporulated oocysts were taken from 20 positive cases by using calibrated eye micrometer. overall incidence was 25.3% (41/162) varying between 15 and 39.5% at differen ... | 1992 | 1561754 |
| pcr-based differentiation of three porcine eimeria species and isospora suis. | isospora suis and eimeria are frequent coccidian parasites of pigs. the unsporulated oocysts of eimeria species and of i. suis are difficult to differentiate. therefore, a species-specific pcr was developed. pcr products were amplified from eimeria polita, eimeria porci, and eimeria scabra using primers from the conserved 18s rrna regions and were subsequently sequenced. based on variable sequence regions, primers were constructed for the differentiation of the three eimeria species and i. suis. ... | 2001 | 11163694 |
| autofluorescence microscopy for the detection of nematode eggs and protozoa, in particular isospora suis, in swine faeces. | parasites from swine faeces were examined for autofluorescence. oocysts of eimeria polita, e. scabra and isospora suis, cysts of balantidium coli and eggs of oesophagostomum dentatum, strongyloides ransomi and trichuris suis (but not those of ascaris suum) emitted light after excitation with uv light. i. suis oocyst counts in mcmaster chambers utilising autofluorescence were compared to those from conventional bright field microscopy. similarly, faecal smears containing i. suis were examined usi ... | 2001 | 11403385 |
| prevalence of coccidian infection in suckling piglets in china. | to determine the prevalence of coccidian infection in suckling piglets in china, fecal samples from 779 litters of suckling piglets were collected on 80 different farms in 17 provinces from september 2009 to december 2010. these samples were examined through saturated saline flotation technique. the prevalences of coccidian infection ranged from 0 to 32.5% among different provinces and the average was 16.7% (130/779). the highest prevalence of 19.9% (69/346) was found in 8-14 day-old litters of ... | 2012 | 22694832 |