| novel human adenovirus causing nosocomial epidemic keratoconjunctivitis. | in 2000, we encountered cases of nosocomial infections with epidemic keratoconjunctivitis (ekc) at a university hospital in kobe, in the western part of japan. two human adenovirus (hadv) strains, kobe-h and kobe-s, were isolated from patients with nosocomial ekc infection. they were untypeable by existing neutralizing antisera; however, the isolate was neutralized with homologous antisera. we then encountered several cases of ekc due to nosocomial infections in eye clinics in different parts of ... | 2008 | 18385435 |
| molecular diagnosis of human adenoviruses d and e by a phylogeny-based classification method using a partial hexon sequence. | human adenoviruses (hadvs) are the major causes of a variety of acute illnesses. virus isolation and neutralization tests are usually done to identify the causative virus, but these tests are labor-intensive and time-consuming, and standardized antisera are in limited supply. this study investigated a rapid and reliable method of virus identification based on pcr and phylogenetic analysis. the phylogenetic tree constructed by neighbor joining on the basis of the newly determined partial hexon se ... | 2004 | 15071007 |
| adenovirus and "culture-negative sepsis" in a preterm neonate. | background respiratory viral infections remain an underrecognized cause of morbidity and mortality among preterm infants in the neonatal intensive care unit (nicu). case report an eight day old, 650 gram birth weight, 23 weeks' gestational age female developed "culture-negative" sepsis manifested by respiratory deterioration, hypoxia, leukocytosis, and thrombocytopenia. she was diagnosed with pneumonia and hepatitis due to adenovirus hadv-d (h29f9) by polymerase chain reaction (pcr) testing, but ... | 2016 | 27924246 |
| recombination analysis of intermediate human adenovirus type 53 in japan by complete genome sequence. | human adenovirus type 53 (hadv-53) has commonly been detected in samples from epidemic keratoconjunctivitis (ekc) patients in japan since 1996. hadv-53 is an intermediate virus, containing hexon-chimeric, penton base and fiber structures similar to hadv-22 and -37, hadv-37 and hadv-8, respectively. hadv-53-like intermediate strains were first isolated from ekc samples in japan in the 1980s. here, the complete genome sequences of three such hadv-53-like intermediate strains (870006c, 880249c and ... | 2011 | 21402595 |
| evidence of molecular evolution driven by recombination events influencing tropism in a novel human adenovirus that causes epidemic keratoconjunctivitis. | in 2005, a human adenovirus strain (formerly known as hadv-d22/h8 but renamed here hadv-d53) was isolated from an outbreak of epidemic keratoconjunctititis (ekc), a disease that is usually caused by hadv-d8, -d19, or -d37, not hadv-d22. to date, a complete change of tropism compared to the prototype has never been observed, although apparent recombinant strains of other viruses from species human adenovirus d (hadv-d) have been described. the complete genome of hadv-d53 was sequenced to elucidat ... | 2009 | 19492050 |
| molecular epidemiology of human adenoviruses d associated with epidemic keratoconjunctivitis in osaka, japan, 2001-2010. | isolation of novel types of human adenovirus d (hadv-53, -54, and -56) from keratoconjunctivitis patients has been reported since 2008. we examined the molecular epidemiology of hadv-d strains using 39 field isolates collected from epidemic keratoconjunctivitis (ekc) patients from 2001 to 2010 in the province of osaka, japan. the molecular types were analyzed by sequencing partial penton base gene, loop 1 in the hexon, and complete fiber genes. of the 39 isolates, the majority were hadv-19 (14/3 ... | 2013 | 24047746 |
| caveolin-1 associated adenovirus entry into human corneal cells. | the cellular entry of viruses represents a critical area of study, not only for viral tropism, but also because viral entry dictates the nature of the immune response elicited upon infection. epidemic keratoconjunctivitis (ekc), caused by viruses within human adenovirus species d (hadv-d), is a severe, ocular surface infection associated with corneal inflammation. clathrin-mediated endocytosis has previously been shown to play a critical role in entry of other hadv species into many host cell ty ... | 2013 | 24147000 |
| high prevalence and diversity of species d adenoviruses (hadv-d) in human populations of four sub-saharan countries. | human adenoviruses of species d (hadv-d) can be associated with acute respiratory illness, epidemic keratoconjunctivitis, and gastroenteritis, but subclinical hadv-d infections with prolonged shedding have also been observed, particularly in immunocompromised hosts. to expand knowledge on hadv-d in sub-saharan africa, we investigated the prevalence, epidemiology and pathogenic potential of hadv-d in humans from rural areas of 4 sub-saharan countries, côte d'ivoire (ci), democratic republic of th ... | 2014 | 24512686 |
| identification of a novel intertypic recombinant species d human adenovirus in a pediatric stem cell transplant recipient. | human adenoviruses (hadv) are known opportunistic pathogens in hematopoietic stem cell transplant (sct) recipients. the detection of hadv infection in children after sct has been implicated as a determinant of poor outcome but specific associations between hadv species or individual hadv types and disease are poorly understood. | 2014 | 25449172 |
| interregional coevolution analysis revealing functional and structural interrelatedness between different genomic regions in human mastadenovirus d. | human mastadenovirus d (hadv-d) is exceptionally rich in type among the seven human adenovirus species. this feature is attributed to frequent intertypic recombination events that have reshuffled orthologous genomic regions between different hadv-d types. however, this trend appears to be paradoxical, as it has been demonstrated that the replacement of some of the interacting proteins for a specific function with other orthologues causes malfunction, indicating that intertypic recombination even ... | 2015 | 25833048 |
| human mastadenovirus type 70: a novel, multiple recombinant species d mastadenovirus isolated from diarrhoeal faeces of a haematopoietic stem cell transplantation recipient. | a human mastadenovirus d (hadv-d) isolated from diarrhoeal faeces of an allogeneic haematopoietic stem cell transplant (sct) recipient was found to be non-typable by sequencing of loops 1 and 2 of the hexon main neutralization epitope ('imputed serology'). in contrast to hadv-c, hadv-d infections are rarely observed in sct patients. therefore, the whole genome of this isolate was sequenced and phylogenetically analysed. in addition, microneutralization testing with type-specific antisera was per ... | 2015 | 26002300 |
| novel human adenovirus strain, bangladesh. | we report a novel human adenovirus d (hadv-65) isolated from feces of 4 children in bangladesh who had acute gastroenteritis. corresponding genes of hadv-65 were related to a hexon gene of hadv-10, penton base genes of hadv-37 and hadv-58, and a fiber gene of hadv-9. this novel virus may be a serious threat to public health. | 2012 | 22515955 |
| genome sequence of a novel virus of the species human adenovirus d associated with acute gastroenteritis. | a novel virus of the species human adenovirus d, hadv-67 (p-new/h9/f25), was first isolated from diarrheal feces of six children in dhaka city, bangladesh. the genome of this novel virus may be composed of multiple recombinations among hadv-9, hadv-25, hadv-26, hadv-33, hadv-46, and an unknown human adenovirus d which was an origin of hadv-67. | 2013 | 23405334 |
| molecular evolution of human adenoviruses. | the recent emergence of highly virulent human adenoviruses (hadvs) with new tissue tropisms underscores the need to determine their ontogeny. here we report complete high quality genome sequences and analyses for all the previously unsequenced hadv serotypes (n = 20) within hadv species d. analysis of nucleotide sequence variability for these in conjunction with another 40 hadv prototypes, comprising all seven hadv species, confirmed the uniquely hypervariable regions within species. the mutatio ... | 2013 | 23657240 |
| computational analysis and identification of an emergent human adenovirus pathogen implicated in a respiratory fatality. | adenoviral infections are typically acute, self-limiting, and not associated with death. however, we present the genomic and bioinformatics analysis of a novel recombinant human adenovirus (hadv-d56) isolated in france that caused a rare neonatal fatality, and keratoconjunctivitis in three health care workers who cared for the neonate. whole genome alignments revealed the expected diversity in the penton base, hexon, e3, and fiber coding regions, and provided evidence for extensive recombination ... | 2010 | 21056888 |
| phylogeny and primary structure analysis of fiber shafts of all human adenovirus types for rational design of adenoviral gene-therapy vectors. | the fiber shaft of human adenoviruses (hadvs) is essential for bringing the penton base into proximity to the secondary cellular receptor. fiber shaft sequences of all 53 hadv types were studied. phylogeny of the fiber shaft revealed clustering corresponding to the hadv species concept. an intraspecies recombination hot spot was found at the shaft/knob boundary, a highly conserved sequence stretch. for example, hadv-d20 clustered with hadv-d23 in the fiber shaft, but with hadv-d47 in the fiber k ... | 2009 | 19656960 |
| molecular characterization of adenoviral infections in cuba: report of an unusual association of species d adenoviruses with different clinical syndromes. | adenoviruses are common pathogens that are responsible for a wide variety of infectious syndromes. the objectives of this study were to identify and characterize members of different adenovirus species at the molecular level and to describe the correlation between viruses and clinical syndromes during a period of 4 years. between 2002 and 2006, 45 of 512 respiratory specimens (8%) from patients with acute respiratory tract infection tested positive for adenovirus. four adenovirus isolates from s ... | 2009 | 19280320 |
| molecular epidemiological study of adenovirus infecting western lowland gorillas and humans in and around moukalaba-doudou national park (gabon). | adenoviruses are widespread in human population as well as in great apes, although the data about the naturally occurring adenovirus infections remain rare. we conducted the surveillance of adenovirus infection in wild western lowland gorillas in moukalaba-doudou national park (gabon), in order to investigate naturally occurring adenovirus in target gorillas and tested specifically a possible zoonotic transmission with local people inhabiting the vicinity of the park. fecal samples were collecte ... | 2016 | 27290717 |
| selection pressure in the human adenovirus fiber knob drives cell specificity in epidemic keratoconjunctivitis. | human adenoviruses (hadvs) contain seven species (hadv-a to -g), each associated with specific disease conditions. among these, hadv-d includes those viruses associated with epidemic keratoconjunctivitis (ekc), a severe ocular surface infection. the reasons for corneal tropism for some but not all hadv-ds are not known. the fiber protein is a major capsid protein; its c-terminal "knob" mediates binding with host cell receptors to facilitate subsequent viral entry. in a comprehensive phylogenetic ... | 2016 | 27512073 |
| computational and serologic analysis of novel and known viruses in species human adenovirus d in which serology and genomics do not correlate. | in november of 2007 a human adenovirus (hadv) was isolated from a bronchoalveolar lavage (bal) sample recovered from a biopsy of an aids patient who presented with fever, cough, tachycardia, and expiratory wheezes. to better understand the isolated virus, the genome was sequenced and analyzed using bioinformatic and phylogenomic analysis. the results suggest that this novel virus, which is provisionally named hadv-d59, may have been created from multiple recombination events. specifically, the p ... | 2012 | 22427992 |
| genomic characterization of human adenovirus 36, a putative obesity agent. | increased levels of serum antibody titers against human adenovirus 36 (hadv-d36) are associated with human obesity and experimental obesity in laboratory animals. while hadv-d36 has been studied as an infectious agent implicated in obesity for over a decade, the complete genome sequence and its analysis have yet to be reported. a detailed analysis of the genome sequence of hadv-d36 may be important to understand its role in obesity. genomic and bioinformatic comparisons with other hadvs identifi ... | 2010 | 20109503 |
| Surveillance of adenovirus D in patients with epidemic keratoconjunctivitis from Fukui Prefecture, Japan, 1995-2010. | Human adenoviruses species D (HAdV-D) are known to cause severe epidemic keratoconjunctivitis. However, the isolation rate of HAdV-D is not high, because HAdV-D is usually slow to propagate. Although new types of HAdV-D have been reported, accurate surveillance has not been performed because of difficulties in culturing the viruses and lack of a practical identification method. In this study, HAdV-Ds were detected and identified from patients with epidemic keratoconjunctivitis in the Fukui Prefe ... | 2012 | 22052618 |
| the e3 cr1-gamma gene in human adenoviruses associated with epidemic keratoconjunctivitis. | human adenovirus species d type 37 (hadv-d37) is an important etiologic agent of epidemic keratoconjunctivitis. annotation of the whole genome revealed an open reading frame (orf) in the e3 transcription unit predicted to encode a 31.6kda protein. this orf, also known as cr1-+¦, is predicted to be an integral membrane protein containing n-terminal signal sequence, luminal, transmembrane, and cytoplasmic domains. hadv-d19 (c), another viral pathogen causing epidemic keratoconjunctivitis, contains ... | 2011 | 21683743 |