| genotyping of uruguayan human adenovirus isolates collected between 1994 and 1998. | adenoviruses are one of the most frequent causative agents of acute lower respiratory infections in infants and young children. twenty-three adenovirus isolates from nasopharyngeal aspirates of children hospitalized for acute lower respiratory infections in uruguay between 1994 and 1998 were studied by restriction enzyme analysis. the genomic analysis showed that 60.9% (n = 14) of isolates belonged to the species human adenovirus c (hadv-c) and 31.9% (n = 9) to the species human adenovirus b (ha ... | 2005 | 16047741 |
| generation of an adenoviral vaccine vector based on simian adenovirus 21. | adenoviral vectors can be used to generate potent humoral and cellular immune responses to transgene products. use of adenoviral vectors based on non-human isolates may allow for their utilization in populations harbouring neutralizing antibodies to common human serotypes. a vector chimera was constructed using simian adenovirus 22 (a serotype belonging to the species human adenovirus e) and simian adenovirus 21 (a serotype belonging to the species human adenovirus b) expressing the ebola (zaire ... | 2006 | 16894185 |
| molecular epidemiology of human adenovirus isolated from children hospitalized with acute respiratory infection in são paulo, brazil. | in a study of acute respiratory disease, two collections of nasopharyngeal aspirates (npa) were obtained from children hospitalized at the pediatric clinic of the university hospital, são paulo, in 1995 and 2000. adenovirus was detected in 33 (8.2%) of 401 children followed. these viruses were isolated in hep-2, hek-293, or nci-h292 cells and serotyped by neutralization. the genome types were determined after restriction analyses of the genomic dna extracted from infected cells. nineteen isolate ... | 2007 | 17177301 |
| in vitro evaluation of the antiviral effects of the homeopathic preparation gripp-heel on selected respiratory viruses. | gripp-heel is a homeopathic preparation frequently used in the treatment of respiratory viral infections such as various types of influenza and the common cold. the antiviral activity of gripp-heel was studied in vitro on human pathogenic enveloped and nonenveloped rna and dna viruses. before the antiviral assays, in vitro cytotoxicity of gripp-heel was determined with cells used for the infection experiments (hela, hep-2, mdck, bgm) as well as with mitogen-stimulated peripheral blood mononuclea ... | 2007 | 18066110 |
| molecular characterization of adenoviral infections in cuba: report of an unusual association of species d adenoviruses with different clinical syndromes. | adenoviruses are common pathogens that are responsible for a wide variety of infectious syndromes. the objectives of this study were to identify and characterize members of different adenovirus species at the molecular level and to describe the correlation between viruses and clinical syndromes during a period of 4 years. between 2002 and 2006, 45 of 512 respiratory specimens (8%) from patients with acute respiratory tract infection tested positive for adenovirus. four adenovirus isolates from s ... | 2009 | 19280320 |
| phylogeny and primary structure analysis of fiber shafts of all human adenovirus types for rational design of adenoviral gene-therapy vectors. | the fiber shaft of human adenoviruses (hadvs) is essential for bringing the penton base into proximity to the secondary cellular receptor. fiber shaft sequences of all 53 hadv types were studied. phylogeny of the fiber shaft revealed clustering corresponding to the hadv species concept. an intraspecies recombination hot spot was found at the shaft/knob boundary, a highly conserved sequence stretch. for example, hadv-d20 clustered with hadv-d23 in the fiber shaft, but with hadv-d47 in the fiber k ... | 2009 | 19656960 |
| infection kinetics of human adenovirus serotype 41 in hek 293 cells. | the purpose of this work was to acquire an overview of the infectious cycle of hadv-41 in permissive hek 293 cells and compare it to that observed with the prototype of the genus, human adenovirus c hadv-2. hek 293 cells were infected with each virus separately and were harvested every 12 h for seven days. infection kinetics were analysed using confocal and electronic microscopy. the results show that, when properly cultivated, hadv-41 was not fastidious. it had a longer multiplication cycle, wh ... | 2009 | 19820835 |
| molecular identification of adenovirus causing respiratory tract infection in pediatric patients at the university of malaya medical center. | there are at least 51 adenovirus serotypes (adv) known to cause human infections. the prevalence of the different human adv (hadv) serotypes varies among different regions. presently, there are no reports of the prevalent hadv types found in malaysia. the present study was undertaken to identify the hadv types associated primarily with respiratory tract infections (rti) of young children in malaysia. | 2010 | 20594359 |
| open reading frame e3-10.9k of subspecies b1 human adenoviruses encodes a family of late orthologous proteins that vary in their predicted structural features and subcellular localization. | subspecies b1 human adenoviruses (hadv-b1s) are important causative agents of acute respiratory disease, but the molecular bases of their distinct pathobiology are still poorly understood. marked differences in genetic content between hadv-b1s and the well-characterized hadv-cs that may contribute to distinct pathogenic properties map to the e3 region. between the highly conserved e3-19k and e3-10.4k/ridĪ± open reading frames (orfs), and in the same location as the hadv-c adp/e3-11.6k orf, hadv-b ... | 2010 | 20739542 |
| beyond bacteria: a study of the enteric microbial consortium in extremely low birth weight infants. | extremely low birth weight (elbw) infants have high morbidity and mortality, frequently due to invasive infections from bacteria, fungi, and viruses. the microbial communities present in the gastrointestinal tracts of preterm infants may serve as a reservoir for invasive organisms and remain poorly characterized. we used deep pyrosequencing to examine the gut-associated microbiome of 11 elbw infants in the first postnatal month, with a first time determination of the eukaryote microbiota such as ... | 2011 | 22174751 |
| Computational analysis of two species C human adenoviruses provides evidence of a novel virus. | Human adenovirus C (HAdV-C) species are a common cause of respiratory infections and can occasionally produce severe clinical manifestations. A deeper understanding of the variation and evolution in species HAdV-C is especially important since these viruses, including HAdV-C6, are used as gene delivery vectors for human gene therapy and in other biotechnological applications. Here, the full-genome analysis of the prototype HAdV-C6 and a recently identified virus provisionally termed HAdV-C57 are ... | 2011 | 21849694 |
| Adenovirus isolation rates in acute flaccid paralysis patients. | Adenoviruses usually cause asymptomatic or mild infection, but occasionally they produce various severe syndromes including neurological disorders. Association of adenovirus infection with acute flaccid paralysis has been investigated. Shedding of adenovirus with feces was detected in 1.05% of young children (mostly infants) with acute flaccid paralysis syndrome versus 0.42% in healthy contact children (P?<?0.01). However, 85% of adenoviruses in the pediatric AFP patients belonged to HAdV-C spec ... | 2012 | 22052705 |
| evaluation of type-specific real-time pcr assays using the lightcycler and j.b.a.i.d.s. for detection of adenoviruses in species hadv-c. | sporadically, hadvs from species hadv-c are detected in acute respiratory disease outbreaks. to rapidly type these viruses, we designed real-time pcr assays that detect and discriminate between adenovirus types hadv-c1, -c2, -c5, and -c6. sixteen clinical isolates from the california department of public health were used to validate the new assays. type-specific taqman real-time pcr assays were designed and used independently to successfully identify 16 representative specimens. the lower limit ... | 2011 | 22046386 |
| dna microarray for detection of gastrointestinal viruses. | gastroenteritis is a clinical illness of humans and other animals that is characterized by vomiting and diarrhea and caused by a variety of pathogens, including viruses. an increasing number of viral species have been associated with gastroenteritis or have been found in stool samples as new molecular tools have been developed. in this work, a dna microarray capable in theory of parallel detection of more than 100 viral species was developed and tested. initial validation was done with 10 differ ... | 2014 | 25355758 |
| intussusception is associated with the detection of adenovirus c, enterovirus b and rotavirus in a rotavirus vaccinated population. | intussusception, a condition where one segment of intestine invaginates into another, occurs predominantly in infants and young children. a number of potential causes have been identified including infectious agents and rotavirus vaccination. following the introduction of rotavirus vaccination of infants in western australia, a laboratory surveillance programme testing notified intussusception cases for infectious agents was commenced. this led to a pcr-based study of the association between gas ... | 2014 | 25464971 |
| chlorine inactivation of hepatitis e virus and human adenovirus 2 in water. | hepatitis e virus (hev) is transmitted via the fecal-oral route and has been recognized as a common source of large waterborne outbreaks involving contaminated water in developing countries. thus, there is the need to produce experimental data on the disinfection kinetics of hev by chlorine in water samples with diverse levels of fecal contamination. here, the inactivation of hev and human adenovirus c serotype 2 (hadv2), used as a reference virus, was monitored using immunofluorescence and quan ... | 2014 | 25252347 |
| enteric viruses and adenovirus diversity in waters from 2016 olympic venues. | rio de janeiro's inner and coastal waters are heavily impacted by human sewage pollution for decades. enteric viruses, including human adenoviruses (hadv), human enterovirus (ev), group a rotavirus (rv) and hepatitis a virus (hav) are more likely to be found in contaminated surface waters. the present work aimed to assess the frequency and loads of ev, hadv-c and -f species, rv and hav in sand and water samples from venues used during the 2016 summer olympics and by tourists attending the event. ... | 2017 | 28185736 |
| respiratory viruses in patients with influenza-like illness in senegal: focus on human respiratory adenoviruses. | human adenoviruses (hadvs) are highly contagious pathogens that are associated with a wide spectrum of human illnesses involving the respiratory tract. in the present study, we investigate the epidemiologic and viral molecular features of hadvs circulating in senegal after 4 consecutive years of sentinel surveillance of influenza-like illness cases. | 2017 | 28328944 |
| molecular epidemiological study of adenovirus infecting western lowland gorillas and humans in and around moukalaba-doudou national park (gabon). | adenoviruses are widespread in human population as well as in great apes, although the data about the naturally occurring adenovirus infections remain rare. we conducted the surveillance of adenovirus infection in wild western lowland gorillas in moukalaba-doudou national park (gabon), in order to investigate naturally occurring adenovirus in target gorillas and tested specifically a possible zoonotic transmission with local people inhabiting the vicinity of the park. fecal samples were collecte ... | 2016 | 27290717 |
| epidemiology and clinical characteristics of respiratory infections due to adenovirus in children living in milan, italy, during 2013 and 2014. | to evaluate the predominant human adenovirus (hadv) species and types associated with pediatric respiratory infections, nasopharyngeal swabs were collected from otherwise healthy children attending an emergency room in milan, italy, due to a respiratory tract infection from january 1 to february 28 of two subsequent years, 2013 and 2014. the hadvs were detected using a respiratory virus panel fast assay (xtag rvp fast v2) and with a hadv-specific real-time polymerase chain reaction; their nucleo ... | 2016 | 27045588 |
| no evidence of a death-like function for species b1 human adenovirus type 3 e3-9k during a549 cell line infection. | subspecies b1 human adenoviruses (hadv-b1) are prevalent respiratory pathogens. compared to their species c (hadv-c) counterparts, relatively little work has been devoted to the characterization of their unique molecular biology. the early region 3 (e3) transcription unit is an interesting target for future efforts because of its species-specific diversity in genetic content among adenoviruses. this diversity is particularly significant for the subset of e3-encoded products that are membrane gly ... | 2012 | 22882760 |
| hydrologic, land cover, and seasonal patterns of waterborne pathogens in great lakes tributaries. | great lakes tributaries are known to deliver waterborne pathogens from a host of sources. to examine the hydrologic, land cover, and seasonal patterns of waterborne pathogens (i.e. protozoa (2), pathogenic bacteria (4) human viruses, (8) and bovine viruses (8)) eight rivers were monitored in the great lakes basin over 29 months from february 2011 to june 2013. sampling locations represented a wide variety of land cover classes from urban to agriculture to forest. a custom automated pathogen samp ... | 2017 | 28187346 |
| phylogenetic evidence for intratypic recombinant events in a novel human adenovirus c that causes severe acute respiratory infection in children. | human adenoviruses (hadvs) are prevalent in hospitalized children with severe acute respiratory infection (sari). here, we report a unique recombinant hadv strain (cbj113) isolated from a hadv-positive child with sari. the whole-genome sequence was determined using sanger sequencing and high-throughput sequencing. a phylogenetic analysis of the complete genome indicated that the cbj113 strain shares a common origin with hadv-c2, hadv-c6, hadv-c1, hadv-c5, and hadv-c57 and formed a novel subclade ... | 2016 | 26960434 |
| differential immunogenicity between hadv-5 and chimpanzee adenovirus vector chadox1 is independent of fiber and penton rgd loop sequences in mice. | replication defective adenoviruses are promising vectors for the delivery of vaccine antigens. however, the potential of a vector to elicit transgene-specific adaptive immune responses is largely dependent on the viral serotype used. hadv-5 (human adenovirus c) vectors are more immunogenic than chimpanzee adenovirus vectors from species human adenovirus e (chadox1 and adc68) in mice, though the mechanisms responsible for these differences in immunogenicity remain poorly understood. in this study ... | 2015 | 26576856 |
| the relative magnitude of transgene-specific adaptive immune responses induced by human and chimpanzee adenovirus vectors differs between laboratory animals and a target species. | adenovirus vaccine vectors generated from new viral serotypes are routinely screened in pre-clinical laboratory animal models to identify the most immunogenic and efficacious candidates for further evaluation in clinical human and veterinary settings. here, we show that studies in a laboratory species do not necessarily predict the hierarchy of vector performance in other mammals. in mice, after intramuscular immunization, hadv-5 (human adenovirus c) based vectors elicited cellular and humoral a ... | 2015 | 25629523 |
| adenovirus infection in savanna chimpanzees (pan troglodytes schweinfurthii) in the issa valley, tanzania. | adenoviruses are a widespread cause of diverse human infections with recently confirmed zoonotic roots in african great apes. we focused on savanna-dwelling chimpanzees in the issa valley (tanzania), which differ from those from forested sites in many aspects of behavior and ecology. pcr targeting the dna polymerase gene detected adv in 36.7% (69/188) of fecal samples. we detected five groups of strains belonging to the species human mastadenovirus e and two distinct groups within the species hu ... | 2018 | 28980083 |
| detection of adenovirus, papillomavirus and parvovirus in brazilian bats of the species artibeus lituratus and sturnira lilium. | bats play a significant role in maintaining their ecosystems through pollination, dispersal of seeds, and control of insect populations, but they are also known to host many microorganisms and have been described as natural reservoirs for viruses with zoonotic potential. the diversity of viruses in these animals remains largely unknown, however, because studies are limited by species, location, virus target, or sample type. therefore, the aim of this study was to detect fragments of viral genome ... | 2019 | 30740637 |