| aromatic polyketide synthases (purification, characterization, and antibody development to benzalacetone synthase from raspberry fruits). | p-hydroxyphenylbutan-2-one, the characteristic aroma compound of raspberries (rubus idaeus l.), is synthesized from p-coumaryl-coenzyme a and malonyl-coenzyme a in a two-step reaction sequence that is catalyzed by benzalacetone synthase and benzalacetone reductase (w. borejsza-wysocki and g. hrazdina [1994] phytochemistry 35: 623-628). benzalacetone synthase condenses one malonate with p-coumarate to form the pathway intermediate p-hydroxyphenylbut-3-ene-2-one (p-hydroxybenzalacetone) in a react ... | 1996 | 12226219 |
| antagonistic properties of two recombinant strains of streptomyces melanosporofaciens obtained by intraspecific protoplast fusion. | intraspecific protoplast fusion was used to produce stable prototrophic recombinants of streptomyces melanosporofaciens ef-76, a biocontrol agent of plant disease producing geldanamycin. two recombinant strains (fp-54 and fp-60) that differed with regard to their antagonistic properties against bacillus cereus atcc 14579, streptomyces scabies ef-35 and phytophthora fragariae var. rubi 390 were characterized. fp-60 lost the ability to inhibit the in vitro growth of these microbial strains while f ... | 2003 | 12883869 |
| glucanolytic actinomycetes antagonistic to phytophthora fragariae var. rubi, the causal agent of raspberry root rot. | a collection of about 200 actinomycete strains was screened for the ability to grow on fragmented phytophthora mycelium and to produce metabolites that inhibit phytophthora growth. thirteen strains were selected, and all produced (beta)-1,3-, (beta)-1,4-, and (beta)-1,6-glucanases. these enzymes could hydrolyze glucans from phytophthora cell walls and cause lysis of phytophthora cells. these enzymes also degraded other glucan substrates, such as cellulose, laminarin, pustulan, and yeast cell wal ... | 1996 | 16535313 |
| gene flow analysis demonstrates that phytophthora fragariae var. rubi constitutes a distinct species, phytophthora rubi comb. nov. | isozyme analysis and cytochrome oxidase sequences were used to examine whether differentiation of p. fragariae var. fragariae and p. fragariae var. rubi at the variety level is justified. in isozyme studies six strains of both p. fragariae varieties were analyzed with malate dehydrogenase (mdh), glucose phosphate isomerase (gpi), aconitase (aco), isocitrate dehydrogenase (idh) and phosphogluconate dehydrogenase (pgd), comprising altogether seven putative loci. five unique alleles (mdh-1(a), mdh- ... | 2007 | 17682774 |
| identification of phytophthora fragariae var. rubi by pcr. | the following chapter describes a pcr method for the identification of the raspberry root rot pathogen phytophthora fragariae var. rubi. furthermore, a nested pcr suitable for the detection of the pathogen in infected raspberry roots and validated against the "duncan bait test" (eppo bull 35:87-91, 2005) is explained. protocols for different dna extraction methods are given which can be transferred to other fungal pathogens. | 2009 | 19301754 |