| evidence of the involvement of a 50s ribosomal protein in several active sites. | the functional role of the bacillus stearothermophilus 50s ribosomal protein b-l3 (probably homologous to the escherichia coli protein l2) was examined by chemical modification. the complex b-l3-23s rna was photooxidized in the presence of rose bengal and the modified protein incorporated by reconstitution into 50s ribosomal subunits containing all other unmodified components. particles containing photooxidized b-l3 are defective in several functional assays, including (1) poly(u)-directed poly( ... | 1975 | 52 |
| proceedings: effect of nutrient limitation on sporulation of bacillus stearothermophillus. | | 1975 | 2695 |
| succinylation of glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. a reactive threonine residue in the apoenzyme. | 1. glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus can be extensively succinylated in the presence of substrates and coenzyme without appreciable loss of activity. 2. the apoenzyme in the absence of substrates is rapidly inhibited by small amounts of succinic anhydride. nad+, glyceraldehyde-3-phosphate and inorganic phosphate all afford protection from inhibition, and inhibition is slowly reversed in the presence of pyrophosphate at ph 8.5. 3. kinetic and spectral studi ... | 1976 | 4305 |
| purification and characterization of inorganic pyrophosphatase from bacillus stearothermophilus. | inorganic pyrophosphatase ec 3.6.1.1 was purified from bacillus stearothermophilus to a homogeneous state both ultracentrifugally and electrophoretically. ultracentrifugal analysis revealed that the molecular weight of the enzyme is 122,000 and the sedimentation coefficient (s0.34%/20, w) is 5.2s. the enzyme molecule in 0.1% sodium dodecylsulfate solution containing 1 mm 2-mercaptoethanol had an estimated molecular weight of 70,000 on the basis of sds-polyacrylamide gel electrophoresis results, ... | 1975 | 5398 |
| the modification of sulfhydryl groups of glutamine synthetase from bacillus stearothermophilus with 5, 5'-dithiobis(2-nitrobenzoic acid). | the sh groups of glutamine synthetase ec 6.3.1.2 from bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of sh groups in the molecule as well as the effect of the modification on the enzyme activity. three sh groups per subunit were detected after complete denaturation of the enzyme with 6 m urea, one of which was essential for the enzyme activity in view of its reactivity with 5, 5'-dithiobis(2-nitrobenzoic acid) on addition of ... | 1975 | 5420 |
| maintainance of specificity, information, and thermostability in thermophilic bacillus sp. glutamine synthetase. | glutamine synthetase has been purified to homogeneity from b. subtilis (37 degrees) b. stearothermophilus (55 degrees), and b. caldolyticus (75 degrees). those characteristics compared include size (6.0 +/- 0.3 x 10(5) daltons), quaternary structure (12 su) amino acid content, substrate km's and specificity for structural analogs, metal ion activation, number and kind of separate feedback modifier sites, and the complexity of modifier-substrate and modifier-modifier site interactions. although t ... | 1976 | 7467 |
| some catalytic and molecular properties of threonine deaminase from bacillus stearothermophilus. | threonine deaminase ec 4.2.1.16 was highly purified from bacillus stearothermophilus. the enzyme exhibited maximum activity at 65 degrees and at ph 9.2--9.6. it was inactivated on dilution and on storage at 4 degrees, but was protected by egg albumin. the enzyme was labile at 65 degrees, but became stable in the presence of egg albumin and isoleucine at ph 7.0. the substrate saturation curve for the enzyme reaction at 40 or 65 degrees was hyperbolic, but in the presence of isoleucine, the curve ... | 1976 | 10288 |
| dtnb modification of sh groups of isocitrate dehydrogenase from bacillus stearothermophilus purified by affinity chromatography. | a new method of affinity chromatography using blue dextran-sepharose 4b resin was established to purify nadp+-dependent isocitrate dehydrogenase ec 1.1.1.42 from bacillus stearothermophilus in high yield. the purified preparation was found to be homogeneous on disc gel electrophoresis. the sh groups of the enzyme were modified with 5,5'-dithiobis-(2-nitrobenzoic acid) (dtnb) to determine the number of sh groups per molecule and their contribution to the enzyme activity. one sh group was titrated ... | 1977 | 14937 |
| a pulse-radiolysis study of the manganese-containing superoxide dismutase from bacillus stearothermophilus. | in the preceding paper the mechanism of catalysis of the manganese-containing superoxide dismutase from bacillus stearothermophilus was shown to involve a 'fast cycle' and a 'slow cycle' mcadam, fox, lavelle & fielden, 1977 (biochem. j. 165, 71-79). further properties of the enzyme was considered in the present paper. pulse-radiolysis studies, under conditions of low substrate concentration to (i.e. when the fast cycle predominates), showed that enzyme activity decreases as ph increases (6.5-10. ... | 1977 | 19013 |
| affinity labeling of adenine nucleotide-related enzymes with reactive adenine nucleotide analogs. ii. affinity labeling of phosphoglycerate kinase with a reactive amp analog. | affinity labeling of yeast and b. stearothermophilus phosphoglycerate kinases with a reactive amp analog, n6-(p-bromoacetaminobenzyl)-amp was examined. complete loss of enzyme activity was observed when 1 mol of the reagent had reacted per mol of either enzyme. results on the effect of ph and substrate addition on the inactivation, titration of sh groups before and after modification, and kinetic studies with amp analogs suggest that the modification occurs at one amino group at or near the subs ... | 1977 | 19433 |
| the identification of lipid acceptor and the biosynthesis of lipid-linked glucose in bacillus stearothermophilus. | | 1977 | 20047 |
| heat stable inhibitors produced in raw milk. | twenty raw farm milk samples were tested for inhibitory substances before and after being kept at room temperature until the ph fell to less than 6.3 but greater than 6.2. the tests were done using the idf-method and the commercial thermocult method. in both methods b. stearothermophilus var. chalidolactis is used as the test organism. in two instances a thermostable inhibitor was produced with an inhibitory potential greater than 0.01 iu/ml penicillin standard. the idf-method was found unsuitab ... | 1977 | 21382 |
| purification and some properties of riboflavin synthetase from bacillus stearothermophilus atcc 8005. | a riboflavin synthetase was purified 51-fold from a thermophilic organism, bacillus stearothermophilus atcc 8005, that grew at 40 to 72 degrees c. some of the properties of the enzyme are: (i) its temperature optimum was 95 degrees c, and the activity was negligible below 40 degrees c; (ii) the arrhenius plot of the initial reaction rates was concave upward, with a break at 65 degrees c, and the apparent activation energies below and above 65 degrees c were 4.2 x 10(4) and 6.7 x 10(4) j/mol, res ... | 1978 | 25043 |
| pyruvate carboxylase from a thermophilic bacillus. studies on the specificity of activation by acyl derivatives of coenzyme a and on the properties of catalysis in the absence of activator. | 1. oxaloacetate synthesis catalysed by pyruvate carboxylase from a thermophilic bacillus in the absence of acetyl-coa required addition of high concentrations of pyruvate, mgatp(2-) and hco(3) (-), and at 45 degrees c occurred at a maximum rate approx. 20% of that in the presence of a saturating concentration of acetyl-coa. the apparent k(m) for hco(3) (-) at ph7.8 was 400mm without acetyl-coa, and 16mm with a saturating activator concentration. the relationship between reciprocal initial rate a ... | 1978 | 25648 |
| purification and properties of acetate kinase from bacillus stearothermophilus. | 1. acetate kinase ec 2.7.2.1 from an thermophile, b. stearothermophilus, was purified and crystalized. 2. this enzyme was shown to be a tetramer of identical subunits which had a molecular weight of about 40,000. amino acid analysis showed no sh group. by analyzing the cd spectrum it was deduced that this enzyme is composed of 36% beta-structure, 21% alpha-helix and 43% unordered structure. 3. this enzyme shared many common enzymatic properties with the counterpart from mesophiles, i.e. ph optim ... | 1978 | 29037 |
| [dna synthesis and degradation in the cells of bacillus stearothermophilus]. | atp-dependent and atp-independent synthesis of dna was studied on nucleotide-permeable cells of bacillus stearothermophilus. the effect of temperature, ph, ionic strength, and bivalent metal ions on both types of dna synthesis was investigated as well as their susceptibility to an sh-blocking agent. the level of atp-independent synthesis of dna in the cells of bac. stearothermophilus was found to be ten times higher than that in e. coli. in the semipermeable cells of bac. stearothermophilus, 90% ... | 1978 | 30883 |
| subunit structure of acetate kinase from bacillus stearothermophilus as studied by hybridization and cross-linking experiments. | | 1978 | 32170 |
| thermostable aminopeptidase from local isolate of bacillus stearothermophilus. | two of 63 isolates from different sources were very active in producing thermostable aminopeptidase. comparing the two isolates, rr-2247 gave the highest enzyme activity and was identified as bacillus stearothermophilus. the enzyme was isolated from the cells by a sonic vibration for 3 minutes at 20 kc/s. the enzyme shows optimum activity over the ph range of 7.5-8.0 at 65 degrees c. the apparent temperature optimum was about 70 degrees c. addition of 0.0001 m co2+ ions stabilized the enzyme in ... | 1979 | 44475 |
| proceedings: the molecular mechanism of action of beta-lactam antibiotics: the solution of an apparent paradox. | | 1975 | 50801 |
| the primary structure of trna phe from bacillus stearothermophilus and its analogy with other trna's from different origins. | | 1975 | 58588 |
| isoleucyl-trna synthetase from bacillus stearothermophilus: specificity of the aminoacylation reaction for aminoacid and trna. | | 1975 | 58626 |
| [proceedings: structure of arginyl-t-rna synthetase sub-units from bacillus stearothermophilus]. | | 1976 | 60939 |
| proceedings: the primary structure of trna2val from bacillus stearothermophilus and its analogy with trnaval species from different origins. | | 1976 | 60956 |
| arginyl-trna synthetase from bacillus stearothermorphilus: characterization of the enzyme in presence of a protease inhibitor [proceedings]. | | 1977 | 68732 |
| studies on the negative circular dichroic bands around 297 nm of ribosomes from bacterial cells. | the small negative cd bands around 297 nm of isolated 30-s and 50-s ribosomal subunits were precisely measured for three bacteria, bacillus stearothermophilus, bacillus subtilis and escherichia coli q 13. the intensities of the negative cd bands of 30-s subunits were always much greater than those of 50-s subunits irrespective of the bacterial strains, which may be related to the difference in comformations of rrnas and proteins in the complexes between these subribosomal particles. the dissocia ... | 1978 | 96858 |
| properties of a thermostable beta-galactosidase from a thermophilic bacillus: comparison of the enzyme activity of whole cells, purified enzyme and immobilised whole cells. | | 1978 | 102872 |
| isolation of a pyrophosphorylase from bacillus subtilis and bacillus stearothermophilus that specifically degrades guanosine 3'-diphosphate 5'-diphosphate. | | 1978 | 103752 |
| disinfection of nitrous oxide inhalation equipment. | cross-infection by contaminated equipment is a potential hazard associated with conscious sedation with nitrous oxide and oxygen . nosocomial infections have occasionally been linked wih the use of unsterile inhalation devices; microbial contamination of sterile nasal hoods routinely occurs during administration of nitrous oxide; and in vitro experiments indicate that subsequent use of contaminated nasal masks may lead to aspiration of microorganisms. although the incidence of respiratory diseas ... | 1979 | 106079 |
| mechanism of penicillin action: penicillin and substrate bind covalently to the same active site serine in two bacterial d-alanine carboxypeptidases. | it has been hypothesized that penicillin acts as a structural analog of the acyl-d-alanyl-d-alanine terminus of nascent bacterial cell wall and that it consequently binds to and acylates the active site of the enzyme(s) that crosslinks the cell wall to form an inactive penicilloyl enzyme [tipper, d.j. & strominger, j.l. (1965) proc. natl. acad. sci. usa 64, 1133-1138]. this study directly proves that penicillin acylates the active site of two penicillin-sensitive enzymes, d-alanine carboxypeptid ... | 1979 | 111240 |
| the guanosine 3',5'-bis(diphosphate) (ppgpp) cycle. comparison of synthesis and degradation of guanosine 3',5'-bis(diphosphate) in various bacterial systems. | | 1979 | 114395 |
| structure and function of l-lactate dehydrogenases from thermophilic and mesophilic bacteria. i) isolation and characterization of lactate dehydrogenases from thermophilic and mesophilic bacilli. | lactate dehydrogenases from thermophilic bacilli (bacillus stearothermophilus, bacillus caldotenax) and from mesophilic bacilli (bacillus x1, bacillus subtilis) have been isolated by a two-step purification procedure. only one type (ldh-p4) composed of four identical subunits (mr 34 000 or 36 000) was found in each bacillus. the tetrameric enzymes were characterized with respect to thermostability, ph and temperature dependence of the pyruvate reduction and the l-lactate oxidation, substrate spe ... | 1979 | 114469 |
| purification by affinity chromatography, properties and crystallisation of phosphofructokinase from thermophilic micro-organisms. | | 1975 | 124670 |
| phosphofructokinase from thermophilic micro-organisms. | | 1976 | 133030 |
| escherichia coli 5s rna binding proteins l18 and l25 interact with 5.8s rna but not with 5s rna from yeast ribosomes. | reconstitution experiments showed that the two escherichia coli 5s rna binding proteins l18 and l25 form a specific complex with yeast 5.8s rna and not with yeast 5s rna. the yeast 5.8s rna-e. coli protein complex was found to exhibit atpase and gtpase activities that had previously been observed for the e. coli 5s rna-protein complex. the tetranucleotide upupcpg, which is an analog of the trna fragment tpsipcpg, interacted strongly with 5s rna-protein complexes from e. coli and bacillus stearot ... | 1977 | 142985 |
| phosphofructokinase from bacillus stearothermophilus [proceedings]. | | 1977 | 143385 |
| structure and control of phosphofructokinase from bacillus stearothermophilus. | the allosteric enzyme phosphofructokinase binds its substrate fructose-6-phosphate between two subunits of the tetramer, and allosteric effectors between another pair of subunits. the effector binding site accommodates both the activator and the inhibitor. the substrate cooperativity and allosteric control are mediated by these ligand bridges between subunits. | 1979 | 156307 |
| [seeking for the mechanism of thermophily and thermostability (author's transl)]. | | 1975 | 167400 |
| the sterilization of gutta-percha points. | the traditional methods employed for the sterilization of gutta-percha points are unsatisfactory. the use of propylene oxide for this purpose is described and experimental evidence is advanced to support this use. | 1975 | 169776 |
| reconstitution of bacillus stearothermophilus 50 s ribosomal subunits from purified molecular components. | bacillus stearothermophilus 50 s ribosomal subunits have been reconstituted from a mixture of purified rna and protein components. the protein fraction of 50 s subunits was separated into 27 components by a combination of various methods including ion exchange and gel filtration chromatography. the individual proteins showed single bands in a variety of polyacrylamide gel electrophoresis systems, and nearly all showed single spots on two-dimensional polyacrylamide gels. the molecular weights of ... | 1976 | 172511 |
| physicochemical characterization of the four-iron-four-sulphide ferredoxin from bacillus stearothermophilus. | 1. a stable ferredoxin was prepared from bacillus stearothermophilus and purified by chromatography on deae-cellulose and by electrophoresis. 2. the minimum molecular weight determined from the amino acid composition was about 7900 and this was in reasonable agreement with a value of 8500 determined by polyacrylamide-gel electrophoresis. the ferredoxin contained four iron atoms and four labile sulphide groups per molecule. 3. the optical absorption, optical-rotatory-dispersion and circular-dichr ... | 1975 | 174558 |
| effect of temperature on the viability of bacillus stearothermophilus. | one of the obligate thermophilic bacteria, bacillus stearothermophilus, was unable to grow at temperatures below 35 degrees c. about 80% of the population in the bacterial culture died at the temperatures, and the same extent of loss in either of the activities of oxygen consumption or synthesis of protein or nucleic acid of the organisms was observed. with the progress of death of the organisms, reduced nicotinamide-adenine dinucleotide came to be oxidized by the organisms, enzymes such as fruc ... | 1976 | 175753 |
| the binding of nicotinamide-adenine dimucleotide to glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. | the binding of nad+ to glyceraldehyde 3-phosphate dehydrogenase (ec 1.2.1.12) from bacillus stearothermophilus has been studied by measurement of protein fluorescence quenching. slight negative co-operativity was observed in the binding of the third and fourth coenzyme molecules to the tetrameric enzyme. the first two coenzyme molecules were tightly bound. in this respect the enzyme resembles that from sturgeon muscle rather than that from yeast. | 1975 | 175789 |
| glyceraldehyde 3-phosphate dehydrogenase from an extreme thermophile, thermus aquaticus. | | 1976 | 181263 |
| oxygen induced transformation of the nadh-nitrate oxidoreductase from bacillus stearothermophilus. | | 1976 | 181264 |
| iodination of glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. | the reaction of iodine with glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus was investigated. the active-site thiol group of the cysteine residue homologous with cysteine-149 in the pig muscle enzyme was protected by reaction with tetrathionate. the apoenzyme was readily inhibited by ki3 solution at ph8, but the coenzyme, nad+, protected the enzyme against inhibition and decreased the extent of iodination. at ph 9.5, ready inhibition of both apo- and holo-enzyme was obs ... | 1976 | 182130 |
| enzyme hyperspecificity. rejection of threonine by the valyl-trna synthetase by misacylation and hydrolytic editing. | valyl-trna synthetase from bacillus stearothermophilus activates thereonine and forms a 1:1 complex with threonyl adenylate, but it does not catalyze the net formation of threonyl-trnaval at ph 7.78 and 25 degrees c in the quenched flow apparatus it decomposes at a rate constant of 36s-1. during this process there is a transient formation of thr-trnaval reaching a maximum at 25 ms and rapidly falling to zero after 150 ms. at the peak, 22% of the (14c) threonine from the complex is present as ( ... | 1976 | 182209 |
| sequence and structure of d-glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. | the glyceraldehyde 3-phosphate dehydogenase holoenzyme of bacillus stearothermophilus possesses precise 222 symmetry: in this respect it differs from the reported structure of the lobster muscle enzyme. pairs of active sites are linked through a flexible polypeptide loop which probably mediates the structural changes giving rise to cooperative effects. three additional salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer. | 1977 | 193030 |
| coenzyme binding and co-operativity in d-glyceraldehyde 3-phosphate dehydrogenase. | | 1977 | 198263 |
| ligand binding stoichiometries, subunit structure, and slow transitions in aminoacyl-trna synthetases. | | 1977 | 199234 |
| uridine-cytidine kinase from novikoff ascites rat tumor and bacillus stearothermophilus. | | 1978 | 211377 |
| purification of alcohol dehydrogenase from bacillus stearothermophilus by affinity chromatography. | | 1978 | 212307 |
| generation of superoxide anions and hydrogen peroxide from beta-lapachone in bacteria. | beta-lapachone markedly increased the generation of superoxide anions and hydrogen peroxide by subcellular membranes of bacillus subtilis and bacillus stearothermophilus. peroxide generation by beta-lapachone was parallel to the inhibition of growth in both microorganisms. | 1978 | 214029 |
| electron spin relaxation of iron-sulphur proteins studied by microwave power saturation. | the electron-spin relaxation of iron-sulphur centres in a range of simple proteins (ferredoxin, high-potential iron-sulphur protein and rubredoxin) was investigated by means of the temperature dependence and microwave power saturation of the epr signal. the proteins containing [2fe-2s] centres all showed temperature optima higher than those for [4fe-4s] centres, but the difference between the slowest-relaxing [4fe-4s] protein (chromatium high-potential iron-sulphur protein) and the fastest-relax ... | 1978 | 215217 |
| a ribosome-independent stringent factor from bacillus stearothermophilus and a low molecular weight substance inhibitory to its activity. | | 1979 | 216581 |
| [structure and function of acetate kinase (author's transl)]. | | 1979 | 228352 |
| studies on the allosteric nature of acetate kinase from bacillus stearothermophilus. | fructose 1,6-bisphosphate (fbp) stimulates the reaction of bacillus stearothermophilus acetate kinase (ak). fbp changes the reaction curve for atp from a sigmoidal type to a michaelis-menten one. the binding of fbp to ak was studied by an equilibrium dialysis method and by measuring changes in fluorescence. the extent of binding of fbp to the enzyme paralleled its activation. in addition, the binding constant for fbp increased in the presence of substrate, atp. these results suggest that fbp is ... | 1979 | 230181 |
| the influence of growth temperature on the heat stability of inorganic pyrophosphatase from crude extracts of bacillus stearothermophilus. | inorganic pyrophosphatase activity of crude extracts from bacillus stearothermophilus adapts its thermostability to the growth temperature of the cells. magnesium ions increase the stability of the enzyme in all cases. depending on the growth temperature of the cells two pyrophosphatase species differing in their molecular weights and heat stabilities have been detected. | 1979 | 232960 |
| glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. | | 1975 | 236448 |
| glucose-6-phosphate isomerase from bacillus stearothermophilus. | | 1975 | 236463 |
| isolation and characterization of isocitrate lyase and malate synthase from bacillus stearothermophilus. | | 1975 | 236948 |
| pyruvate carboxylase from bacillus stearothermophilus: molecular size, biotin content and subunit constitution. | | 1975 | 236949 |
| effect of ph on sporulation of bacillus stearothermophilus. | an improved broth medium was developed for high growth yields of bacillus subtilis var. niger ncib 8649, bacillus cereus ncib 9373, and bacillus stearothermophilus ncib 8919 and atcc 7953. sporulation was abundant (1.1 times 10-8 b. subtilis var. niger and 9.2 times 10-7 b. cereus per ml) at an initial ph of 7.0. sporulation of both strains of b. stearothermophilus took place (1.9 times 10-7 and 2.4 times 10-7/ml, respectively) in this medium when initial ph values of 7.7 to 8.7 were used. | 1975 | 238470 |
| synthesis of polyglycerol phosphate by bacillus stearothermophilus. | a particulate enzyme preparation from bacillus stearothermophilus synthesized 1,3-poly(glycerol phosphage) from cdpglycerol at an optimum ph of 8.0 and the reaction was stimulated by divalent cations. km for cdpglycerol was 0.18 mm. the synthesis was inhibited by cmp, cdp, and ctp and by concentrations of cdp-glycerol above 0.49 mm. the reaction was irreversible, the product had an average chain length of 8 glycerol units. about two thirds of the polymers were synthesized in entirety while the r ... | 1975 | 238960 |
| biochemical changes during sporulation of bacillus stearothermophilus. | the process of sporulation was studied in bacillus stearothermophilus. a medium is described that supports good growth and sporulation of the organism. in this medium, which contains glucose, salts, and amino acids, acetate starts to accumulate before any of the glucose is catabolized. enzymes of the tricarboxylic acid cycle are present at all times during growth and sporulation and are found in dormant spores. as the glucose in the culture is consumed, acetate rapidly increases and the ph of th ... | 1975 | 240496 |
| affinity chromatography on immobilised nucleotides. some applications to the purification of thermophilic dehydrogenases and kinases. | the effect of ph and temperature on the capacity and binding of bacillus stearothermophilus, alcohol dehydrogenase and phosphofructokinase to n6-(6-aminohexyl)-5'-amp-sepharose has been examined. specific elution from the substituted amp-sepharose was examined using a variety of cofactors, fragments of cofactors and substrates. a purification scheme for each enzyme on the substituted amp-sepharose using nucleotides and gradients of ph and salt is presented. interestingly, elevated temperature in ... | 1975 | 240692 |
| polypeptide synthesis inhibition by a factor inducing stabilization of 30 s-50 s ribosomal couples. | | 1978 | 250480 |
| effect of storage conditions on the performance of bacillus stearothermophilus biological indicators. | | 1979 | 260940 |
| cluster characterization in iron-sulfur proteins by magnetic circular dichroism. | we report magnetic circular dichroism (mcd) spectra of 4-fe iron-sulfur clusters in the iron-sulfur proteins chromatium high-potential iron protein (hipip), bacillus stearothermophilus ferredoxin and clostridium pasteurianum ferredoxin. the mcd is found to vary significantly with cluster oxidation state but is relatively insensitive to the nature of the protein. the spectra obtained are compared with the corresponding spectra of iron-sulfur proteins containing 2-fe clusters. it is concluded tha ... | 1978 | 281679 |
| [genetics and biochemistry of the bacterial ribosome]. | | 1977 | 322729 |
| structural requirements of the gdp binding site of elongation factor tu. | | 1977 | 323049 |
| the n-terminal sequence of superoxide dismutase from the strict anaerobe desulfovibrio desulfuricans. | | 1977 | 323056 |
| the rates of evolution in some ribosomal components. | the rate of nucleotide substitution (k(nuc)) of 5s rna was estimated to be (1.8 +/- 0.5) x 10(-10) per site per year by comparing the nucleotide sequences of human and xenopus 5s rna and using the geological time elapsed since the separation of mammals and amphibians. similarly, k(nuc) of 5.8s rrna was calculated to be 0.93 10(-1u) per site per year from the sequences of rat hepatoma cells and saccbaromyces cerevisiae. for the comparison of these data with the amino acid substitution rate of kn ... | 1977 | 325217 |
| 3' terminal sequences of 16s rrna do not explain translational specificity differences between e. coli and b. stearothermophilus ribosomes. | | 1977 | 327330 |
| ribosomal protein s1/s1a in bacteria. | | 1977 | 330231 |
| energy expenditure in the editing mechanism of protein synthesis [proceedings]. | | 1977 | 332559 |
| sequence homologies between the tryptophanyl trna synthetases of bacillus stearothermophilus and escherichia coli. | | 1977 | 334569 |
| [studies on the structure and function of proteins--with special reference to glyceraldehyde 3-phosphate dehydrogenase (author's transl)]. | | 1977 | 336812 |
| [structure and function of ef-tu and ef-ts (author's transl)]. | | 1977 | 336813 |
| binding oligonucleotides to escherichia coli and bacillus stearothermophilus 5 s rna. | | 1978 | 347090 |
| [3-phosphoglycerate kinase from microorganisms (author's transl)]. | | 1977 | 347503 |
| the proton exchange of the pro-s hydrogen atom at c-1 in dihydroxyacetone phosphate and d-fructose 1,6-bisphosphate catalysed by class-i and class-ii aldolases. | the efficacy of class-i and class-ii aldolases in catalysing the c-1 proton exchange in fructose 1,6-bisphosphate and dihydroxyacetone phosphate was investigated. the rate of this reaction was at least two orders of magnitude slower in class-ii than in the class-i aldolases. it is suggested that this difference reflects the formation of different intermediates in the reactions catalysed by the two classes of aldolase. | 1978 | 352341 |
| identification of escherichia coli and bacillus stearothermophilus ribosomal protein binding sites on escherichia coli 5s rna. | e coli [32p]-labelled 5s rna was complexed with e. coli and b. stearothermophilus 50s ribosomal proteins. limited t1 rnase digestion of each complex yielded three major fragments which were analysed for their sequences and rebinding of proteins. the primary binding sites for the e. coli binding proteins were determined to be sequences 18 to 57 for e-l5, 58 to 100 for e-l18 and 101 to 116 for e-l25. rebinding experiments of purified e. coli proteins to the 5s rna fragments led to the conclusion t ... | 1978 | 353489 |
| an enhanced thermostability in thermophilic 5-s ribonucleic acids under physiological salt conditions. | the secondary structure of 5-s rrnas of thermus aquaticus (an extreme thermophile), bacillus stearothermophilus (a moderate thermophile) and escherichia coli (a mesophile) was compared using thermal denaturation techniques under varying ionic conditions. at a low ionic strength (10 mm k+), the tm of t. aquaticus 5-s rna differed by only 1 degrees c from that of e. coli rna and the molecule was fully denatured well below the optimum growth temperature of the thermophile. the internal na+, k+ and ... | 1978 | 363159 |
| mechanisms of thermophily. | | 1978 | 365460 |
| potentiometric titrations and oxidation-reduction potentials of manganese and copper-zinc superoxide dismutases. | bovine erythrocyte superoxide dismutase and two manganese-containing superoxide dismutases have been reduced by the indirect coulometric titration method with methylviologen as the mediator-titrant. on the basis of the titration data the manganese-containing superoxide dismutases contain 1 g-atom of metal per mol of enzyme (dimer). e0' = +0.31 v for the enzyme from escherichia coli which exhibits a complicated ph dependence above neutral ph. the bacillus stearothermophilus manganese-containing e ... | 1979 | 380641 |
| dna-dependent rna polymerase from the archaebacterium sulfolobus acidocaldarius. | purified dna-dependent rna polymerase from sulfolobus acidocaldarius is composed of 10 different subunits, one of which is present as four copies. their molecular weights are 122 000, 101 000, 44 000, 32 000, 24 000, 17 500, 13 800, 11 800 (four copies), 11 200, 10 800, summing up to a total mr of 423 500. the sedimentation velocity is 13.5 s, indicating that at 0.5 m nh4cl the enzyme exists in the monomeric form. at ph 9.2 in cellogel electrophoresis two of the subunits migrate towards the cath ... | 1979 | 380989 |
| conformation and biological activity of acidic ribosomal proteins from different organisms. | | 1979 | 389163 |
| kinetic evidence for an acyl-enzyme intermediate in d-alanine carboxypeptidases of bacillus subtilis and bacillus stearothermophilus. | the kinetics of hydrolysis and transpeptidation of the synthetic substrate diacetyl-l-lysyl-d-alanyl-d-alanine and of the natural substrate udp-acetylmuramyl pentapeptide and related compounds catalyzed by the d-alanine carboxypeptidases of bacillus subtilis and bacillus stearothermophilus in the presence of the nucleophiles hydroxylamine or glycine have been examined. these kinetic data suggest that an acyl-enzyme intermediate is formed in the first step of the reaction and that the transpeptid ... | 1977 | 404297 |
| gardimycin, a new antibiotic inhibiting peptidoglycan synthesis. | gardimycin, a new antibiotic, at 100 mug/ml, specifically inhibited cell wall synthesis and induced accumulation of uridine 5'-diphosphate-n-acetylmur-amylpentapeptide in whole cells of bacillus subtilis. the antibiotic was active in a particulate enzyme preparation from bacillus stearothermophilus: 60 mug/ml caused 50%, and 200mug/ml caused 100%, inhibition of peptidoglycan synthesis. suppression of peptidoglycan synthesis was accompanied by parallel accumulation of the lipid intermediate. this ... | 1977 | 404960 |
| [biological indicators: research on the resistance ratio between b. stearothermophilus and bacillus subtilis and pumilis]. | | 1977 | 405247 |
| microwave oven irradiation as a method for bacterial decontamination in a clinical microbiology laboratory. | exposure of 10 frequently isolated clinical pathogens to microwave irradiation resulted in total sterilization with 60 s. time exposure experiments done with commercially prepared test strips containing bacillus stearothermophilus spores indicated that 5-min exposure was adequate to insure sterility of small, contaminated loads. | 1977 | 410828 |
| inactivation of bacillus spores in dry systems at low and high temperatures. | a plot of the thermal resistance of bacillus subtilis var. niger spores (log d value) against temperature was linear between 37 and 190 degrees c (z = 23 degrees c), provided that the relative humidity of the spore environment was kept below a certain critical level. the corresponding plot for bacillus stearothermophilus spores was linear in the range 150 to 180 degrees c (z = 29 degrees c) but departed from linearity at lower temperatures (decreasing z value). however, the z value of 29 degrees ... | 1977 | 411885 |
| [methods of demonstrating antibiotics residues in meat, organs and eggs]. | | 1977 | 416557 |
| further evidence for the structure of the teichoic acids from bacillus stearothermophilus b65 and bacillus subtilis var. niger wm. | bacillus stearothermophilus b65 and bacillus subtilis var. niger wm both contain teichoic acids in their walls composed of glycerol, phosphate and glucose. the 13c nuclear magnetic resonance spectrum of b. stearothermophilus teichoic acid showed 13c-31p coupling on the signals from the c-5 and c-6 carbon atoms of the glucose molecule and an alpha-glucosidic linkage between glucose and the c-1 atom of the glycerol moiety. these data are consistent with a poly[glucosylglycerol phosphate] as the ce ... | 1978 | 417919 |
| determination of base pairing in escherichia coli and bacillus stearothermophilus 5s rnas by infrared spectroscopy. | the extent of base pairing in escherichia coli and bacillus stearothermophilus 5s rnas was determined by infrared spectroscopy. from the infrared spectra taken at 20 degrees and 52 degrees c it is concluded that e. coli and b. stearothermophlius 5s rnas possess a large number of base pairs (table i). comparison of our results with those previously published using other methods leads to the conclusion that the structures of prokaryotic 5s rnas involve a large number of tertiary interactions, in w ... | 1979 | 388350 |
| adenosine triphosphate consumption by bacterial arginyl-transfer ribonucleic acid synthetases. | atp consumption by arginyl-trna synthetases from escherichia coli and bacillus stearothermophilus has been investigated by the firefly luciferin--luciferase assay. arginyl-trna synthetase from e. coli utilizes atp only for aminocylation of trna with a 1:1 stoicheiometry. in contrast, we have shown an adenosine triphosphatase activity of arginyl-trna synthetase from b. stearothermophilus in the absence of trnaarg. dowex chromatography revealed the formation of adp by the thermophile enzyme; under ... | 1979 | 384995 |
| improved procedure of purification of inorganic pyrophosphatase from bacillus stearothermophilus. | an improved method for isolation of inorganic pyrophosphatase (ec 3.6.1.1) from bacillus stearothermophilus is described. the enzyme was purified to more than 90% after two chromatographic steps. a molecular weight of 140,000 daltons was estimated by density gradient centrifugation. the isoelectric point was found to be 4.0. | 1979 | 429108 |
| identification of the amino acid residue modified in bacillus stearothermophilus alcohol dehydrogenase by the nad+ analogue 4-(3-bromoacetylpyridinio)butyldiphosphoadenosine. | 4-(3-bromoacetylpyridinio)butyldiphosphoadenosine was synthesized with a [carbonyl-14c]acetyl label. the reactive coenzyme analogue inactivates alcohol dehydrogenase from bacillus stearothermophilus by forming a covalent enzyme-coenzyme compound. the inactivation kinetics as well as the spectral properties of the modified enzyme after treatment with sodium hyposulphite suggest that the analogue is bound at the coenzyme binding site. b. stearothermophilus alcohol dehydrogenase modified with 14c-l ... | 1979 | 436831 |
| evidence for the double-sieve editing mechanism in protein synthesis. steric exclusion of isoleucine by valyl-trna synthetases. | | 1979 | 375976 |
| respiration, oxidative phosphorylation and yields in aerobic bacteria. | | 1979 | 437299 |