| complete nucleotide sequence of cherry necrotic rusty mottle virus. | a virus closely related to cherry green ring mottle virus (cgrmv) was isolated from a tree displaying typical symptoms of cherry necrotic rusty mottle disease. we have named this virus cherry necrotic rusty mottle virus (cnrmv) and report here its complete genomic sequence as determined from overlapping cloned cdnas. cnrmv has a genome of 8,432 nucleotides excluding the 3' poly(a) sequence and codes for 7 significant open reading frames (orfs). five of these orfs are conserved among all fovea-, ... | 2001 | 11315647 |
| molecular characterization of foveaviruses associated with the cherry necrotic mottle leaf disease and complete sequencing of an european isolate of cherry green ring mottle virus. | analysis of the p1c124 source (associated with the cherry necrotic mottle leaf (cnml) disease) revealed the presence of two different viral agents. the complete nucleotide sequence of one of these agents, p1a, had an overall nucleotide sequence similarity of 83% with a previously sequenced north american isolate of cherry green ring mottle virus (cgrmv) and should therefore be regarded as an european isolate of cgrmv. approximately 2 kb of the 5' end of the genome of the second agent, p1b, were ... | 2002 | 12021871 |
| an improved reverse transcription-polymerase chain reaction (rt-pcr) assay for the detection of two cherry flexiviruses in prunus spp. | a one-step reverse transcription-polymerase chain reaction (rt-pcr) procedure was developed to detect cherry green ring mottle virus (cgrmv) and cherry necrotic rusty mottle virus (cnrmv) in woody indicators and naturally infected prunus spp. viral rna suitable for rt-pcr was obtained by a simple trapping method that did not require either extraction of double-stranded rna (dsrna) or total rna, availability of virus antibodies, or purification of viral particles. consensus primers, degenerate pr ... | 2005 | 15996763 |
| characterization of a flowering cherry strain of cherry necrotic rusty mottle virus. | the host range and complete nucleotide sequences of two cherry necrotic rusty mottle virus (cnrmv) isolates (fc4 and fc5) infecting flowering cherry accessions imported from japan are described. of the plants tested, cherry, peach, apricot and almond became infected, but only sweet cherry cv. 'canindex', nanking cherry and apricot cv. 'tilton' showed a mild foliar mottle. the genomic sequences of cnrmv-fc4 and cnrmv-fc5 are 8,430 and 8,429 nt in length, excluding the 3' poly (a) tail. they conta ... | 2008 | 18368286 |
| reverse transcription-polymerase chain reaction-based detection of plant viruses. | a one-step reverse transcription-polymerase chain reaction (rt-pcr) is used to detect two cherry flexiviruses, cherry green ring mottle virus (cgrmv) and cherry necrotic rusty mottle virus (cnrmv), in prunus species. this unit presents procedures for collection of plant samples, preparation of total nucleic acids, viral rna-rich or total rna extracts from plant tissues, and subsequent amplification of the viral targets by one-step rt-pcr using a pair of consensus primers. the pcr amplicons are v ... | 2007 | 18770613 |
| survey of cherry necrotic rusty mottle virus and cherry green ring mottle virus incidence in korea by duplex rt-pcr. | the incidence of cherry necrotic rusty mottle virus (cnrmv) and cherry green ring mottle virus (cgrmv) have recently been occurred in korea, posing a problem for sweet cherry cultivation. since infected trees have symptomless leaves or ring-like spots on the pericarp, it is difficult to identify a viral infection. in this study, the incidence of cnrmv and cgrmv in sweet cherry in gyeongbuk province was surveyed using a newly developed duplex reverse transcriptase polymerase chain reaction (rt-pc ... | 2014 | 25506311 |
| genomic analyses of cherry rusty mottle group and cherry twisted leaf-associated viruses reveal a possible new genus within the family betaflexiviridae. | it is demonstrated that closely related viruses within the family betaflexiviridae are associated with a number of diseases that affect sweet cherry (prunus avium) and other prunus spp. cherry rusty mottle-associated virus (crmav) is correlated with the appearance of cherry rusty mottle disease (crmd), and cherry twisted leaf-associated virus (ctlav) is linked to cherry twisted leaf disease (ctld) and apricot ringpox disease (arpd). comprehensive analysis of previously reported full genomic sequ ... | 2015 | 25496302 |
| simultaneous detection of cherry necrotic rusty mottle virus and cherry green ring mottle virus using real-time pcr and high resolution melting analysis. | in this study, the real-time pcr assays were combined with high resolution melting (hrm) analysis for the simultaneous detection of cherry necrotic rusty mottle virus (cnrmv) and cherry green ring mottle virus (cgrmv) infection in sweet cherry trees. detection of cnrmv and cgrmv was performed in a real-time pcr using a primer set for both of them or duplex real-time pcr that included one specific primer set for each virus. these two strategies allowed us to confirmed virus infection in all teste ... | 2014 | 24675146 |
| viruses associated with rusty mottle and twisted leaf diseases of sweet cherry are distinct species. | virus rna sequences related to those of the family betaflexiviridae were amplified from trees affected with the following diseases: cherry twisted leaf, apricot ring pox, cherry necrotic rusty mottle, cherry rusty mottle, and cherry green ring mottle. phylogenetic analysis of virus sequences obtained from these diseased trees from western north america, along with published sequences of cherry green ring mottle virus (cgrmv) and cherry necrotic rusty mottle virus (cnrmv), revealed four major cla ... | 2013 | 24219146 |
| complete nucleotide sequence of a virus associated with rusty mottle disease of sweet cherry (prunus avium). | cherry rusty mottle is a disease of sweet cherries first described in 1940 in western north america. because of the graft-transmissible nature of the disease, a viral nature of the disease was assumed. here, the complete genomic nucleotide sequences of virus isolates from two trees expressing cherry rusty mottle disease symptoms are characterized; the virus is designated cherry rusty mottle associated virus (crmav). the biological and molecular characteristics of this virus in comparison to thos ... | 2013 | 23525699 |
| simultaneous detection and identification of four cherry viruses by two step multiplex rt-pcr with an internal control of plant nad5 mrna. | a multiplex reverse transcription-polymerase chain reaction (mrt-pcr) was developed and standardized for the simultaneous detection of four cherry viruses: cherry virus a (cva, genus; capillovirus), cherry necrotic rusty mottle virus (cnrmv, unassigned species of the betaflexiviridae), little cherry virus 1 (lchv-1, genus; closterovirus) and prunus necrotic ringspot virus (pnrsv, genus; ilarvirus) with nad5 as plant internal control. a reliable and quick method for total plant rna extraction fro ... | 2013 | 23707922 |