| construction of an agroinfectious clone of bean rugose mosaic virus using gibson assembly. | construction of agroinfectious viral clones usually requires many steps of cloning and sub-cloning and also a binary vector, which makes the process laborious, time-consuming, and frequently susceptible to some degree of plasmid instability. nowadays, novel methods have been applied to the assembly of infectious viral clones, and here we have applied isothermal, single-step gibson assembly (ga) to construct an agroinfectious clone of bean rugose mosaic virus (brmv) using a small binary vector. t ... | 2017 | 28315991 |
| complete genome sequence of bean rugose mosaic virus, genus comovirus. | since the first report in costa rica in 1971, bean rugose mosaic virus (brmv) has been found in colombia, el salvador, guatemala and brazil. in this study, the complete genome sequence of a soybean isolate of brmv from paraná state, brazil, was determined. the brmv genome consists of two polyadenylated rnas. rna1 is 5909 nucleotides long and encodes a single polypeptide of 1856 amino acids (aa), with an estimated molecular weight of 210 kda. the rna1 polyprotein contains the polypeptides for vir ... | 2016 | 26973227 |
| inclusion bodies induced by bean rugose mosaic comovirus seen under light microscopy. | two types of inclusion bodies were consistently observed under light microscopy in bean (phaseolus vulgaris) leaf tissue infected with bean rugose mosaic virus (brmv), a species of the genus comovirus, family comoviridae. one type consisted of vacuolated inclusions found mainly in the cytoplasm of epidermal cells. the other type consisted of abundant crystalloid inclusions of different sizes and shapes found consistently in glandular hairs, guard cells, phloem tissue, xylem elements and occasion ... | 2000 | 11487940 |