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comparison of the mode of action and site specificity of endo-(1,4)-beta-d-glucanases of penicillium pinophilum using normal, 1-3h-labelled, reduced and chromogenic cello-oligosaccharides. 19902276486
the mechanism of fungal cellulase action. synergism between enzyme components of penicillium pinophilum cellulase in solubilizing hydrogen bond-ordered cellulose.studies on reconstituted mixtures of extensively purified cellobiohydrolases i and ii and the five major endoglucanases of the fungus penicillium pinophilum have provided some new information on the mechanism by which crystalline cellulose in the form of the cotton fibre is rendered soluble. it was observed that there was little or no synergistic activity either between purified cellobiohydrolases i and ii, or, contrary to previous findings, between the individual cellobiohydrolases and the endo ...19892549957
cell-growth and migration inhibition of human mesothelioma cells induced by 3-o-methylfunicone from penicillium pinophilum and cisplatin.malignant pleural mesothelioma is a fatal malignancy linked to asbestos exposure. the main challenge for mesothelioma treatment is to go beyond the drug resistance, in particular against cisplatin (cddp), one of the most used chemotherapeutic drug. 3-o-methylfunicone (omf) is a metabolite produced by the fungus penicillium pinophilum; its antiproliferative properties have been previously studied in vitro. particularly, omf is able to inhibit mesothelioma cell motility. to improve the effects of ...201121656164
production of monapinones by fermentation of the dinapinone-producing fungus penicillium pinophilum fki-3864 in a seawater-containing medium.five new monapinones, including a dinapinone monomer, were isolated from the culture broth of the dinapinone-producing penicillium pinophilum fki-3864 in a medium modified to contain seawater. the structures of these monapinones were elucidated by various nmr experiments. monapinones possessed the same dihydronaphthopyranone skeleton as the dinapinones, with different hydroxyalkyl chains: monapinone a was identified as the monomeric portion of the atropisomer dinapinones a1 and a2, and monapinon ...201121610716
fungal root endophytes of the carnivorous plant drosera rotundifolia.as carnivorous plants acquire substantial amounts of nutrients from the digestion of their prey, mycorrhizal associations are considered to be redundant; however, fungal root endophytes have rarely been examined. as endophytic fungi can have profound impacts on plant communities, we aim to determine the extent of fungal root colonisation of the carnivorous plant drosera rotundifolia at two points in the growing season (spring and summer). we have used a culture-dependent method to isolate fungal ...201020012108
3-o-methylfunicone, a metabolite of penicillium pinophilum, inhibits proliferation of human melanoma cells by causing g(2) + m arrest and inducing apoptosis.melanoma cells take advantage of impaired ability to undergo programmed cell death in response to different external stimuli and chemotherapeutic drugs; this makes prevention of tumour progression very difficult. the aim of this study was to demonstrate whether 3-o-methylfunicone (omf), a metabolite of penicillium pinophilum, has the ability to arrest cell population growth and to induce apoptosis in a375p (parental) and a375m (metastasis derivatived) melanoma cell lines.200919486013
arbuscular mycorrhizae formed by penicillium pinophilum improve the growth, nutrient uptake and photosynthesis of strawberry with two inoculum-types.penicillium pinophilum was isolated from the soil in a commercial strawberry field. the strain readily formed arbuscular mycorrhizae (am) with the roots of strawberry 'zoji' (fragaria x ananassa duch. cv.) when plants were inoculated with either fresh cultured hyphae or root/soil mixtures. fresh hyphae, however, resulted in higher amounts of colonization than root/soil inoculum. compared with uninoculated strawberries, inoculation increased plant dry weight by 31%, as well as nitrogen content (4 ...200818483699
dinapinones, novel inhibitors of triacylglycerol synthesis in mammalian cells, produced by penicillium pinophilum fki-3864.dinapinone a, a novel biaryl dihydronaphthopyranone, was isolated from the culture broth of penicillium pinophilum fki-3864 by solvent extraction, silica gel and ods column chromatography and hplc. dinapinone a showed very potent inhibition of triacylglycerol (tg) synthesis in intact chinese hamster ovary k1 (cho-k1) cells with an ic(50) value of 0.097 μm. dinapinone a was found to be a mixture of stereoisomers, resulting in its separation into dinapinones a1 and a2 by hplc using a c30 reverse-p ...201121559025
the cellulase system of the anaerobic rumen fungus neocallimastix frontalis: studies on the properties of fractions rich in endo-(1-->4)-beta-d-glucanase activity.seven fractions rich in endoglucanase activity were separated from the extracellular cellulase system of the anaerobic rumen fungus neocallimastix frontalis. the fractions (es1, es3, es2u1, es2u2, es2u4, es2u3c1 and es2u3c2) were separated from each other and from a fraction that could solubilize crystalline cellulose (the so-called crystalline-cellulose-solubilizing component, ccsc) by the sequential use of differential adsorption on the microcrystalline cellulose avicel, gel filtration and aff ...19958579830
3-o-methylfunicone, from penicillium pinophilum, is a selective inhibitor of breast cancer stem cells.cancer stem cells make up a subpopulation of cells within tumours that drive tumour initiation, growth and recurrence. they are resistant to many current types of cancer treatment, causing failure of such therapeutic approaches, including chemotherapy and radiotherapy. in the study described here, anti-proliferative effects of 3-o-methylfunicone (omf), a metabolite from penicillium pinophilum, were investigated on human breast cancer mcf-7 cells and cancer stem cells selected as mammospheres der ...201121951283
3-o-methylfunicone, a metabolite produced by penicillium pinophilum, modulates erk1/2 activity, affecting cell motility of human mesothelioma cells.3-o-methylfunicone (omf), a secondary metabolite produced by penicillium pinophilum, affects cell proliferation and motility in a variety of human solid tumours. the aim of this study was to demonstrate whether omf has the ability to arrest cell division and motility, in a human mesothelioma cell line. malignant mesothelioma is an aggressive cancer that does not respond to standard therapies the cells of which are considered to be highly resistant to apoptosis.201020447056
3-o-methylfunicone, a secondary metabolite produced by penicillium pinophilum, induces growth arrest and apoptosis in hela cells.3-o-methylfunicone (omf) is a secondary metabolite produced by the soil fungus penicillium pinophilum which has cytostatic properties. the aim of this study was to investigate the mechanisms by which such properties are exerted, with special reference to any anti-proliferative and apoptotic potential, on hela cells. omf treatment caused about 44% inhibition of cell growth after 24 h, and modifications in the tubulin fibre organization. in addition, a significant increase in p21 mrna expression a ...200415548174
impact of phosphate-solubilizing fungi on the yield and phosphorus-uptake by wheat and faba bean plants.three fungal isolates (phosphate-dissolvers), aspergillus niger, a. fumigatus and penicillium pinophilum were isolated from the rhizosphere of different plants grown in ismailia and south sinai governorates. they effectively solubilized rock phosphate or tricalcium phosphate in pikovskaya's liquid medium. in pot and column experiments, they significantly reduced ph and increased available phosphorus in the soil treated with either rock phosphate or superphosphate. the yield components of wheat a ...200011061191
purification and characterization of a feruloyl/p-coumaroyl esterase from solid-state cultures of the aerobic fungus penicillium pinophilum. 19921426561
acidic β-mannanase from penicillium pinophilum c1: cloning, characterization and assessment of its potential for animal feed application.the β-mannanase gene, man5c1, was cloned from penicillium pinophilum c1, a strain isolated from the acidic wastewater of a tin mine in yunnan, china, and expressed in pichia pastoris. the sequence analysis displayed the gene consists of a 1221-bp open reading frame encoding a protein of 406 amino acids (man5c1). the deduced amino acid sequence of man5c1 showed the highest homology of 57.8% (identity) with a characterized β-mannanase from aspergillus aculeatus belonging to glycoside hydrolase fam ...201122036533
inactivation effects of uv irradiation and ozone treatment on the yeast and the mold in mineral water.in recent years, bottled mineral water has undergone inactivation by methods other than the traditional heat treatment during the production process; there are fewer reports of the effectiveness of these inactivation methods on yeasts and molds in mineral water than on bacteria and protozoan oocysts. in this study, we evaluated the effects of uv irradiation and ozone treatment compared with heat treatment at 85 degrees c on yeast cells and mold spores inoculated into mineral water. a 5-log reduc ...201020819369
hydrolysis of cellulose derived from steam exploded bagasse by penicillium cellulases: comparison with commercial cellulase.a complete cellulase from penicillium pinophilum was evaluated for the hydrolysis of alpha-cellulose derived from steam exploded sugarcane bagasse and other cellulosic substrates. alpha-cellulose at 1% substrate concentration was completely hydrolyzed by penicillium cellulase within 3h wherein at 10% the hydrolysis was 100% within 24 h with an enzyme loading of 10 fpu/g. the hydrolysate yielded glucose as major end product as analyzed by hplc. under similar conditions, hydrolysis of sigmacell (m ...200919683917
enhancement of metabolites productivity of penicillium pinophilum fki-5653, by co-culture with trichoderma harzianum fki-5655.in the course of discovering new metabolites from co-culture of penicillium pinophilum fki-5653 and trichoderma harzianum fki-5655, a new compound, designated secopenicillide c, and four known compounds, penicillide, mc-141, pestalasin a and stromemycin were isolated. the production of these compounds, except pestalasin a, was enhanced in co-culture to be two to six times higher than in pure culture of penicillium pinophilum fki-5653, which was the producer of these compounds.201122008698
hydrolysis of the polysaccharides of straw by enzymes produced by a mutant strain of the fungus penicillium pinophilum.the saccharification of the polysaccharides of barley, oat, and wheat straws and solka floc was studied using the extracellular enzyme system synthesized by mutant strain ntg iii/6 of the fungus penicillium pinophilum 87160iii. the enzymes obtained in cultures containing solka floc or barley straw as the carbon source were compared. solka floc at 10% (w/v) concentration was hydrolyzed to the extent of 70% in 72 h at 50 degrees c using a reaction mixture containing 7 filter paper units/ml of cell ...198918587933
growth and enzyme production by three penicillium species on monosaccharides.the growth and preference for utilisation of various sugar by the penicillium species penicillium pinophilum ibt 4186, penicillium persicinum ibt 13226 and penicillium brasilianum ibt 20888 was studied in batch cultivations using various monosaccharides as carbon source, either alone or in mixtures. p. pinophilum ibt 4186 and p. persicinum ibt 13226 had a micro(max) around 0.08-0.09 h(-1) using either glucose or xylose as carbon source. the micro(max) of p. brasilianum ibt 20888 was 0.16 and 0.1 ...200415066767
structure elucidation of a novel funicone-like compound produced by penicillium pinophilum.a novel metabolite related to the previously identified compound 3-o-methylfunicone (1) was isolated from the fungus penicillium pinophilum and its structure elucidated by spectral analysis as 3-o-methyl-5, 6-epoxyfunicone (2).200212049222
cytostatic properties of a novel compound derived from penicillium pinophilum: an in vitro study.3-o-methylfunicone (omf), a secondary metabolite produced by penicillium pinophilum, inhibits the in vitro growth of plant pathogenic fungi. this specific property suggested that the compound could be used against other fungal pathogenic activities, including dermatological ones. however, for such applications, toxicological side-effects should be taken into account, in order to prevent other types of risk to mammalian cells. therefore, investigations were made of the basic toxicity of omf towar ...200211827571
the cellulase of penicillium pinophilum. synergism between enzyme components in solubilizing cellulose with special reference to the involvement of two immunologically distinct cellobiohydrolases.two immunologically unrelated cellobiohydrolases (i and ii), isolated from the extracellular cellulase system elaborated by the fungus penicillum pinophilum, acted in synergism to solubilize the microcrystalline cellulose avicel; the ratio of the two enzymes for maximum rate of attack was approx. 1:1. a hypothesis to explain the phenomenon of synergism between two endwise-acting cellobiohydrolases is presented. it is suggested that the cellobiohydrolases may be two stereospecific enzymes concern ...19863707549
fungal cellulase systems. comparison of the specificities of the cellobiohydrolases isolated from penicillium pinophilum and trichoderma reesei.reaction patterns for the hydrolysis of chromophoric glycosides from cello-oligosaccharides and lactose by the cellobiohydrolases (cbh i and cbh ii) purified from trichoderma reesei and penicillium pinophilum were determined. they coincide with those found for the parent unsubstituted sugars. cbh i enzyme from both organisms attacks these substrates in a random manner. turnover numbers are, however, low and do not increase appreciably as a function of the degree of polymerization of the substrat ...19892803246
pcr-rflp of its rdna for the rapid identification of penicillium subgenus biverticillium species.rflp of its rdna is proposed as a useful tool for molecular identification of the most common species of biverticillate penicillia. 60 isolates were analysed representing 13 species and 21 unique sequences were produced. the combination of five restriction enzymes was successful in separating 12 species. however, the variety penicillium purpurogenum var. rubrisclerotium remained indistinguishable from penicillium funiculosum. p. funiculosum appeared as the most confused species, being mis-identi ...200617196020
characterization of endo-beta-1,4-glucanase from a novel strain of penicillium pinophilum kmj601.a novel endo-beta-1,4-glucanase (eg)-producing strain was isolated and identified as penicillium pinophilum kmj601 based on its morphology and internal transcribed spacer (its) rdna gene sequence. when rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, the maximal eg activity of 5.0 u mg protein(-1), one of the highest levels among eg-producing microorganisms, was observed. the optimum temperature and ph for eg production were 28 degrees c and 5.0, respectiv ...201019575195
3-o-methylfunicone produced by penicillium pinophilum affects cell motility of breast cancer cells, downregulating alphavbeta5 integrin and inhibiting metalloproteinase-9 secretion.recent evidence assigns integrins and metalloproteinases (mmps) an important role in regulating tumor cell progression. here, we demonstrate that 3-o-methylfunicone (omf), a secondary metabolite produced by penicillium pinophilum, affects cell proliferation and motility of breast cancer mcf-7 cells, downregulating alphavbeta5 integrin, and inhibiting mmp-9 secretion. this effect was absent when the non-tumoral mcf-10 cell line was used. inhibition of cell motility was also associated to modifica ...200717562555
pinophilins a and b, inhibitors of mammalian a-, b-, and y-family dna polymerases and human cancer cell proliferation.pinophilins a (1) and b (2), new hydrogenated azaphilones, and sch 725680 (3) were isolated from cultures of a fungus (penicillium pinophilum hedgcok) derived from a seaweed, and their structures were determined using spectroscopic analyses. these compounds selectively inhibited the activities of mammalian dna polymerases (pols), a (pol γ), b (pols α, δ, and ε), and y (pols η, ι, and κ) families, but did not influence the activities of the four x-family pols (pols β, λ, μ, and terminal deoxynucl ...201222264170
study of the mode of action and site-specificity of the endo-(1----4)-beta-d-glucanases of the fungus penicillium pinophilum with normal, 1-3h-labelled, reduced and chromogenic cello-oligosaccharides.the modes of action of the five major endo-(1----4)-beta-d-glucanases (i, ii, iii, iv and v) purified from penicillium pinophilum cellulase were compared by h.p.l.c. analysis, with normal, 1-3h-labelled and reduced cello-oligosaccharides and 4-methylumbelliferyl glycosides as substrates. significant differences were observed in the preferred site of cleavage even when substrates with the same number of glycosidic bonds were compared. thus, although endoglucanase i was unable to attack normal cel ...19902317193
multiple forms of endo-1,4-beta-d-glucanase in the extracellular cellulase of penicillium pinophilum.the influence of the composition of the growth medium on the production of endo-1,4-beta-d-glucanase (cm-cellulase) activity by p. pinophilum was studied in shake flask cultures using avicel ph101 as the carbon source. it was observed that the culture conditions had a profound effect on the level of endoglucanase (cm-cellulase) produced by p. pinophilum. however, isoelectric focusing of the endoglucanase activity obtained from shake flask and fermenter cultures using the same growth medium revea ...198918587856
computerized screening for novel producers of monascus-like food pigments in penicillium species.monascus pigments have been used as natural food colorants in asia for centuries. they are not authorized for use in the european union and the united states mainly due to the risk of coproduction of the mycotoxin citrinin by monascus spp. in the present study, we screened for novel producers of monascus-like pigments from ascomycetous filamentous fungi belonging to penicillium subgenus biverticillium that are not reported to produce citrinin or any other known mycotoxins. the screening was carr ...200818841978
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