Publications

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a light and electron microscopic study of sensory organs and associated structures in the foreleg tibia of the cricket, gryllus assimilis. 19724636814
an electron microscopic study of the tympanal organ and associated structures in the foreleg tibia of the cricket, gryllus assimilis. 19724636815
biochemical characterization of juvenile hormone esterases from lines selected for high or low enzyme activity in gryllus assimilis.in a previous study, activity of the insect endocrine regulator juvenile hormone esterase (jhe), in the cricket gryllus assimilis, was subjected to bidirectional selection. this resulted in three pairs of high- and low-selected lines, each of which differed by 3.5-fold in jhe activity. in the present study, juvenile hormone esterases from these lines were characterized with respect to the michaelis constant (k(m)), thermostability, and inhibition. none of three high-selected jhes differed from i ...19969126671
the role of wolbachia bacteria in reproductive incompatibilities and hybrid zones of diabrotica beetles and gryllus crickets.a rickettsial bacterium in the genus wolbachia is the cause of a unidirectional reproductive incompatibility observed between two major beetle pests of maize, the western corn rootworm, diabrotica virgifera virgifera, and the mexican corn rootworm, d. v. zeae. these subspecies are allopatric except for two known regions of sympatry in texas and mexico. we demonstrate that populations of d. v. virgifera, with the exception of two populations in southern arizona, are infected with a strain of wolb ...19979326628
purification and characterization of hemolymph juvenile hormone esterase from the cricket, gryllus assimilis.juvenile hormone esterase (jhe) from the serum of the cricket, gryllus assimilis, was purified to homogeneity in a four-step procedure involving polyethylene glycol precipitation, hydrophobic interaction fplc, and ion exchange fplc. this procedure could be completed in 4 days and resulted in a greater than 900-fold purification with greater than 30% recovery. the purified enzyme exhibited a single band on a silver-stained sds page gel and had an apparent subunit molecular mass of 52 kda. the nat ...200211754093
biochemical aspects of flight and flightlessness in gryllus: flight fuels, enzyme activities and electrophoretic profiles of flight muscles from flight-capable and flightless morphs.female gryllus assimilis subjected to 4.5-7.7h continuous tethered flight had significantly lower amounts of total lipid, triglyceride and total soluble carbohydrate compared with unflown controls. a much greater amount of total lipid (6.3mg) was used during flight compared with carbohydrate (0.14mg). flown individuals also had substantially reduced amounts of injected, radiolabeled [(14)c]-oleic acid. activities of lipid, carbohydrate and amino acid catabolizing enzymes in flight muscles of g. ...199912770375
eicosanoids mediate nodulation reactions to bacterial infections in adults of the cricket, gryllus assimilis.nodulation is the temporally and quantitatively most important cellular defense reaction to bacterial infections in insects. inhibition of eicosanoid biosynthesis in adults of the cricket, gryllus assimilis, immediately prior to intrahemocoelic injections of the bacterium, serratia marcescens, sharply reduced the nodulation response. separate treatments with specific inhibitors of phospholipase a(2), cyclooxygenase, and lipoxygenase reduced nodulation, supporting our view that nodule formation i ...199912770398
animal-based folk remedies sold in public markets in crato and juazeiro do norte, ceará, brazil.human communities consistently develop a detailed knowledge of the therapeutical and medicinal properties of the local flora and fauna, and these folk remedies often substitute medicines produced by the pharmaceutical industry. animals (and their derived products) are essential ingredients in the preparation of many traditional remedies. the present work prepared an inventory of the animals sold in public markets in the cities of crato and juazeiro do norte, ceará state, brazil.200919493345
cellular immunosenescence in adult male crickets, gryllus assimilis.ecological immunity studies in invertebrates, particularly insects, have generated new insights into trade-offs between immune functions and other physiological parameters. these studies document physiologically directed reallocations of immune costs to other high-cost areas of physiology. immunosenescence, recognized as the age-related deterioration of immune functions, is another mechanism of radically altering immune systems. we investigated the hypothesis that aging brings on immunosenescenc ...201121254201
evolutionary endocrinology of juvenile hormone esterase in gryllus assimilis: direct and correlated responses to selection.hemolymph juvenile hormone esterase (jhe) activity on the third day of the last stadium in the cricket, gryllus assimilis, exhibited a significant response to selection in each of six replicate lines. mean realized heritability was 0.26 +/- 0.04. the response was due to changes in whole-organism enzyme activity as well as to changes in the proportion of enzyme allocated to the hemolymph compartment. in vivo juvenile hormone metabolism differed between some lines selected for high vs. low enzyme ...19958582618
susceptibility of north-american and european crickets to acheta domesticus densovirus (addnv) and associated epizootics.the european house cricket, acheta domesticus l., is highly susceptible to a. domesticus densovirus (addnv). commercial rearings of crickets in europe are frequently decimated by this pathogen. mortality was predominant in the last larval stage and young adults. infected a. domesticus were smaller, less active, did not jump as high, and the adult females seldom lived more than 10-14 days. the most obvious pathological change was the completely empty digestive caecae. infected tissues included ad ...201021167171
early embryonic development of the head region of gryllus assimilis fabricius, 1775 (orthoptera, insecta).we report our investigations on the embryonic development of gryllus assimilis, with particular attention to the head. significant findings revealed with scanning electron microscopy (sem) images include: (1) the pre-antennal lobes represent the anterior-most segment that does not bear any appendages; (2) each of the lobes consists of central and marginal regions; (3) the central region thereof develops into the protocerebrum and the optic lobes, whereas the marginal region thereof becomes the a ...201020558319
experimental infection of selected arthropods with spirurid nematodes spirocerca lupi railliet & henry, 1911 and gongylonema ingluvicola molin, 1857.gongylonema ingluvicola and spirocerca lupi are spirurid nematodes that require arthropod intermediate hosts in order to complete their life cycle. beetles of the family scarabaeidae are reported to serve as intermediate hosts for both these parasites. in this study selected species of beetles of the family scarabaeidae as well as other groups of arthropods were screened for susceptibility to infection with s. lupi and g. ingluvicola. arthropods were exposed to infective eggs of both parasites f ...201020132587
the physiology of life-history trade-offs: experimental analysis of a hormonally induced life-history trade-off in gryllus assimilis.adult gryllus assimilis given an analog of juvenile hormone exhibited reduced flight muscles and enlarged ovaries similar to those found in naturally occurring flightless individuals of species that are polymorphic for dispersal capability. control and hormone-treated (flightless) g. assimilis did not differ in the amount of food consumed or assimilated on any of three diets that differed in nutrient quantity. thus, enhanced ovarian growth of flightless individuals resulted from increased alloca ...199818811398
tissue and stage-specific juvenile hormone esterase (jhe) and epoxide hydrolase (jheh) enzyme activities and jhe transcript abundance in lines of the cricket gryllus assimilis artificially selected for plasma jhe activity: implications for jhe microevolution.fat body and midgut juvenile hormone esterase (jhe) and juvenile hormone epoxide hydrolase (jheh) specific activities, and plasma jhe activity, were measured throughout the last stadium in two pairs (blocks) of lines of the cricket gryllus assimilis, each pair of which had been artificially selected for high- or low-plasma jhe activity. highly significant differences were observed between high- and low-activity lines of each block on most days for fat body jhe, and on one day for midgut jhe acti ...200818634793
jhe in gryllus assimilis: cloning, sequence-activity associations and phylogeny.the 458 amino acid sequence of a mature jhe protein from the cricket gryllus assimilis was identified after isolating the partial cdna sequence encoding this protein from a fat body and midgut cdna library. this hemimetabolan jhe sequence shows over 40% amino acid similarity to the known jhe sequences of several holometabolous insects. it also includes previously determined peptide sequences for g. assimilis jhe as well as two other motifs associated with jhe enzymes in holometabolous insects. t ...200717967354
toxicity of venoms from vipers of pelias group to crickets gryllus assimilis and its relation to snake entomophagy.the existing data indicate that snake venom is most toxic towards the natural vertebrate preys. several species of snake include arthropods in their food. however, there is no available data on the toxicity of venom from entomophagous snakes towards their prey. we have studied the toxicity of venom from vipers of pelias group towards crickets gryllus assimilis. the pelias group includes several closely related viper species inhabiting mainly the south european part of russia, and they differ in ...200717341427
prevalence and intensity of cephalobium microbivorum (nematoda: diplogasterida) infection in three species of gryllus field crickets.here we report on the results of a survey for nematode parasites in three species of field cricket, gryllus integer, gryllus lineaticeps, and an undescribed gryllus species. the nematode, cephalobium microbivorum, was recovered from the intestine of the crickets. to our knowledge, this paper is just one of two to report on the biology of c. microbivorum. this nematode was first described from the cricket, gryllus assimilis. it has not been documented in any other gryllus species to date. g. inte ...200516052362
the first representative of glycosylated three-fingered toxins. cytotoxin from the naja kaouthia cobra venom.there are different glycosylated proteins in snake venoms, but no glycosylated representatives of a large family of three-fingered toxins have previously been detected. a new glycoprotein was isolated from the venom of the thai cobra naja kaouthia. maldi ms of the glycoprotein contained an array of peaks in the range from approximately 8900 to approximately 9400 da indicating its microheterogeneity. carbohydrate analysis showed the presence of mannose, galactose, n-acetylglucosamine, fucose and ...200415128311
the limiting mechanism in tarsal chemoreception.rejection thresholds of eight primary alcohols applied to the tarsal chemoreceptors of the blowfly phormia regina meigen and the ovipositor of gryllus assimilis fab. have been determined. three different solvents for the alcohols have been used: water, ethylene glycol, and mineral oil. the comparative stimulating effectiveness of the alcohols assumes a different aspect with each different solvent. in oil the range of thresholds from methanol to octanol extends over less than one log unit as comp ...195114873921
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