[controlling effect of parasitoids on population of vegetable leaf miner liriomyza sativae blanchard].the controlling effect of parasitoids on vegetable leaf miner, liriomyza sativae blanchard, was evaluated by using acting factor life tables and exclusion inex of population control(epic). the results showed that chrysocharis pentheus, neuchrysocharis formosa, chrysonotomyia okazakii, hemiptarsenus varicornis, chrysocharis sp., opius dimidiatus and opius dissitus played an important role in controlling the population of vegetable leaf miner. the eipcs of parasitoids were 1.23, 2.73, 5.98, 5.92 a ...200111757375
toxicity of chemicals commonly used in indonesian vegetable crops to liriomyza huidobrensis populations and the indonesian parasitoids hemiptarsenus varicornis, opius sp., and gronotoma micromorpha, as well as the australian parasitoids hemiptarsenus varicornis and diglyphus isaea.liriomyza huidobrensis (blanchard) and liriomyza sativae (blanchard) are important pests of vegetable crops in indonesia and are likely to spread to neighboring countries. three pesticides (dimehypo, abamectin, and cyromazine) are currently used to control these pests, but there is little information on their effectiveness against field populations and on their impact on parasitoids controlling liriomyza species. the toxicity of these chemicals to l. huidobrensis and three common parasitoids (he ...200415384326
juvenile and sublethal effects of selected pesticides on the leafminer parasitoids hemiptarsenus varicornis and diglyphus isaea (hymenoptera: eulophidae) from australia.the pest leafminers liriomyza huidobrensis (blanchard), liriomyza sativae (blanchard), and liriomyza trifolii (burgess) (diptera: agromyzidae) have spread into south east asia and oceania, and they are likely to reach australia in the near future. two translaminar pesticides, cyromazine and abamectin, currently provide effective chemical control of these pests, but because parasitoids can play an important role in controlling and preventing leafminer outbreaks, understanding the impact of pestic ...200516539101
use of monoclonal antibodies in an elisa for detecting an invasive pest insect, liriomyza trifolii (diptera: agromyzidae).a monoclonal antibody was prepared by the hybridoma technology. it reacted only with the protein of liriomyza trifolii (burgess) and not with that of chromatomyia horticola goureau or liriomyza sativae blanchard in indirect enzyme-linked immunosorbent assay. it was effective even after being diluted more than 8.192×10(6)-fold. the detection sensitivity of the antibody was 31.3 µg/ml under controlled conditions. positive reaction was achieved with all laboratory-reared l. trifolii samples, includ ...201526470159
new sources of resistance to leafminers (liriomyza sativae) in melon (cucumis melo l.) germplasm.leafminers (liriomyza sativae) are the main melon (cucumis melo l.) pests in northeast brazil, which is the main region for the production and export of the fruit in brazil. of the integrated management strategies available, genetic resistance is the best method of preventing damage by these insects. the aim of this study was to select sources of resistance to leafminers in melon germplasm. seven experiments were conducted in the laboratory, field, and greenhouse, with and without choice, using ...201728387878
thermal requirement and development of liriomyza sativae (diptera: agromyzidae) on cucumber.the effect of temperature on developmental rate of vegetable leafminer, liriomyza sativae blanchard (diptera: agromyzidae), was determined at seven constant temperatures (10, 15, 20, 25, 30, 35, and 40 degrees c) on cucumber, cucumis sativus l. 'negin'. the total developmental period (oviposition to adult emergence) decreased with increasing temperature, although no development occurred at 10 and 40 degrees c. using the linear model, the estimated lower temperature threshold for the egg, larval, ...200717461057
species composition, distribution, and seasonal abundance of liriomyza leafminers (diptera: agromyzidae) under different vegetable production systems and agroecological zones in kenya.a longitudinal study to identify the species of liriomyza leafminer, their distribution, relative abundance, and seasonal variation, including their host range, was conducted in vegetable fields at three altitudes in kenya from november 2011 to november 2012. three main species were identified: liriomyza huidobrensis (blanchard), liriomyza sativae blanchard, and liriomyza trifolii (burgess), of which l. huidobrensis was the most abundant across all altitudes irrespective of the cropping season a ...201526313175
[suppressive effect of secondary substances on liriomyza sativae population].life table and interference index of population control (iipc) were used to evaluate the effects of spraying alcohol extracts of plant secondary substances on the population of liriomyza sativae. field experiment results showed that alcohol extracts derived from lantana camara, eupatorium odoratum, and wedelia chinensis, and one kind of commercial petroleum spray oil were positive to protect kidney bean from infestation of l. sativae. the iipc of these repelling substance compared with control w ...200312722448
insecticide-mediated apparent displacement between two invasive species of leafminer fly.closely related invasive species may often displace one another, but it is often difficult to determine mechanisms because of the historical nature of these events. the leafmining flies liriomyza sativae and liriomyza trifolii have become serious invasive agricultural pests throughout the world. where both species have invaded the same region, one predominates over the other. although l. sativae invaded hainan island of china first, it recently has been displaced by the newly invasive l. trifoli ...201222662122
local crop planting systems enhance insecticide-mediated displacement of two invasive leafminer fly.liriomyza sativae and l. trifolii are highly invasive leafminer pests of vegetable crops that have invaded southern china in recent years. liriomyza sativae was the first of these species to invade china, but it is now being displaced by l. trifolii. the rate and extent of this displacement vary across southern china. in hainan, monocultures of highly valuable cowpea are planted and treated extensively with insecticides in attempts to control leafminer damage. in guangdong, cowpea fields are int ...201424651465
does temperature-mediated reproductive success drive the direction of species displacement in two invasive species of leafminer fly?liriomyza sativae and l. trifolii (diptera: agromyzidae) are two highly invasive species of leafmining flies, which have become established as pests of horticultural crops throughout the world. in certain regions where both species have been introduced, l. sativae has displaced l. trifolii, whereas the opposite has occurred in other regions. these opposing outcomes suggest that neither species is an inherently superior competitor. the regions where these displacements have been observed (souther ...201424906119
temperature-dependent development of two neotropical parasitoids of liriomyza sativae (diptera: agromyzidae).we studied two species of neotropical parasitoids that occur naturally in northeastern brazil and are associated with liriomyza sativae (blanchard): opius (gastrosema) scabriventris nixon (hymenoptera: braconidae) and chrysocharis vonones (walker) (hymenoptera: eulophidae). we evaluated the influence of seven temperatures on the duration of the egg-adult period and on the survivorship of the immature stages of the parasitoids. a temperature increase from 15 to 30°c shortened the egg-adult period ...201425434037
genetic differentiation of geographical populations of liriomyza sativae (diptera: agromyzidae) in china based on mitochondrial coi gene this study, partial sequences of the mitochondrial cytochrome oxidase subunit i (coi) gene of four liriomyza sativae (blanchard) geographic populations in china were sequenced. as for the 784 bp mtdna-coi gene obtained, six variable sites were found which were all transitions and no base composition was insertions or deletions. six haplotypes were identified in all the sequences, with five showing polymorphism and one was exclusive. nucleotide diversity (π) and haplotype diversity (h) ranged ...201625489776
abamectin resistance in strains of vegetable leafminer, liriomyza sativae (diptera: agromyzidae) is linked to elevated glutathione s-transferase activity.abamectin resistance was selected in the vegetable leafminer, liriomyza sativae (blanchard) (diptera: agromyzidae) under laboratory conditions, and cross-resistance patterns and possible resistance mechanisms in the abamectin-resistant strains (al-r, af-r) were investigated. compared with the susceptible strain (ss), strain al-r displayed 39-fold resistance to abamectin after 20 selection cycles during 25 generations, and strain af-r exhibited 59-fold resistance to abamectin after 16 selection c ...201525813391
dna-based identifications reveal multiple introductions of the vegetable leafminer liriomyza sativae (diptera: agromyzidae) into the torres strait islands and papua new guinea.leafmining flies (diptera: agromyzidae) can be serious economic pests of horticultural crops. some genera such as liriomyza are particularly problematic with numerous species, some of which are highly polyphagous (wide host range), which can only be confidently identified morphologically from adult males. in our study, dna barcoding was employed to establish new locality records of the vegetable leafminer fly, liriomyza sativae, from the islands of torres strait (queensland, australia) and the c ...201525991411
incidence and characterization of resistance to fenpropathrin in some liriomyza sativae (diptera: agromyzidae) populations in iran.the vegetable leafminer, liriomyza sativae blanchard, is a key pest of vegetable and ornamental crops worldwide and is mainly controlled by insecticides. the toxicity of fenpropathrin on adult flies of f1 generation of six iranian populations of the pest was assessed using the residual glass vial method. the bioassay results showed significant differences in susceptibility to fenpropathrin among the populations tested. resistance ratios ranged from 5.09 to 28.32. the resistant populations were r ...201426309281
selection for chlorpyrifos resistance in liriomyza sativae blanchard: cross-resistance patterns, stability and biochemical mechanisms.the vegetable leafminer (vlm), liriomyza sativae (diptera: agromyzidae) is a serious pest of vegetable crops and ornamentals worldwide. in cropping systems with inappropriate management strategies, development of resistance to insecticides in leafminers is probable. chlorpyrifos is a commonly used pesticide for controlling leafminers in iran, but resistance to this insecticide in leafminers has not been characterized. in order to develop strategies to minimize resistance in the field and greenho ...201526453235
wing-kinematics measurement and aerodynamics in a small insect in hovering flight.wing-motion of hovering small fly liriomyza sativae was measured using high-speed video and flows of the wings calculated numerically. the fly used high wingbeat frequency (≈265 hz) and large stroke amplitude (≈182°); therefore, even if its wing-length (r) was small (r ≈ 1.4 mm), the mean velocity of wing reached ≈1.5 m/s, the same as that of an average-size insect (r ≈ 3 mm). but the reynolds number (re) of wing was still low (≈40), owing to the small wing-size. in increasing the stroke amplitu ...201627168523
screening of melon genotypes for resistance to vegetable leafminer and your phenotypic correlations with colorimetry.melon is one of the most important vegetable crops in the world. with short cycle in a system of phased planting, phytosanitary control is compromised, and a great volume of agricultural chemicals is used to control vegetable leafminer. genetic control is an ideal alternative to avoid the damage caused by this insect. thus, the aim of this study was to evaluate cucumis accessions in regard to resistance to leafminer and correlate the variables analyzed. fifty-four accessions and four commercial ...201828640355
electrophysiological and behavioral responses of a parasitic wasp to plant volatiles induced by two leaf miner the present study, y-tube olfactometric assays demonstrated that headspace volatile extracts collected from leaf miner-damaged, or artificially damaged, bean plants were more attractive to naive females of the parasitoid insect opius dissitus than those collected from healthy plants. headspace extracts from both liriomyza huidobrensis and liriomyza sativae second-instar larvae-damaged beans were analyzed by coupled gas chromatography-electroantennographic detection (gc-ead). of nine ead-activ ...200616621971
evolution of hsp70 gene expression: a role for changes in at-richness within disparate organisms adaptation to thermal stress has been linked to changes in the expression of genes encoding heat-shock proteins (hsp). the underlying genetics, however, remain elusive. we show here that two at-rich sequence elements in the promoter region of the hsp70 gene of the fly liriomyza sativae that are absent in the congeneric species, liriomyza huidobrensis, have marked cis-regulatory consequences. we studied the cis-regulatory consequences of these elements (called atrs1 and atr ...201121655251
differential effects of pesticide applications on liriomyza huidobrensis (diptera: agromyzidae) and its parasitoids on pea in central kenya.three liriomyza species [liriomyza huidobrensis (blanchard), liriomyza trifolii (burgess), and liriomyza sativae blanchard] have been reported as the most important leafminer pests in vegetable production systems in africa. in kenya, farmers rely on indiscriminate synthetic insecticides use. on-farm field investigations were set up at three different locations (sagana, kabaru, and naromoru) in central kenya to determine the effect of pesticide application on the abundance of leafminers and their ...201526470177
what limits the distribution of liriomyza huidobrensis and its congener liriomyza sativae in their native niche: when temperature and competition affect species' distribution range in guatemala.factors limiting distribution range for most species are generally unknown regardless of whether they are native or invasive. we studied factors that could enable or restrict the distribution of two cosmopolitan invasive leafminer fly species, liriomyza huidobrensis (blanchard) and liriomyza sativae (blanchard) in their native niche. in order to test which ecological and environmental factors affect leafminer distribution we conducted thermal tolerance assays, sampled along elevation gradients a ...201728973492
[analysis and identification of liriomyza sativae-attractants from cowpea and kidney bean volatiles].in this paper, the volatiles from cowpea and kidney bean, the main host plants of american leafminer liriomyza sativae, were collected and extracted by a self-designed device and solid phase microextraction (spme) technique, and analyzed by gc-ms. the results showed that the volatiles from the two beans had the same components mainly consisted of 2-hexenal, 3-hexen-1-ol, 2-hexen-1-ol, 1-octen-3-ol, 3-hexenol acetate, alpha-ionone and beta-ionone. the lure made of the seven components was attract ...200516110669
volatiles released from bean plants in response to agromyzid flies.liriomyza sativae blanchard and liriomyza huidobrensis (blanchard) (diptera: agromyzidae) are two invasive flies in china that have caused economical damage on vegetables and ornamental plants. in this article, we report the profiles of emitted volatiles from healthy, mechanically damaged, and leafminer-damaged bean, phaseolus vulgaris l., plants. among 25 emitted volatiles identified, (e)-2-hexen-1-al, (3e)-4,8-dimethyl-1,3,7-nonatriene (dmnt), (z)-3-hexenyl acetate, (z)-3-hexen-1-ol, (syn)- an ...200616404576
population genetic structure and migration patterns of liriomyza sativae in china: moderate subdivision and no bridgehead effect revealed by microsatellites.while liriomyza sativae (diptera: agromyzidae), an important invasive pest of ornamentals and vegetables has been found in china for the past two decades, few studies have focused on its genetics or route of invasive. in this study, we collected 288 l. sativae individuals across 12 provinces to explore its population genetic structure and migration patterns in china using seven microsatellites. we found relatively low levels of genetic diversity but moderate population genetic structure (0.05 < ...026615869
lifetime gains and patterns of accumulation and mobilization of nutrients in females of the synovigenic parasitoid, diglyphus isaea walker (hymenoptera: eulophidae), as a function of nature, adult parasitoids feed to obtain and use nutrients for supplementing and/or replenishing some of their existing array of nutrient reserves. when adults feed on host or non-host food, they can enhance fitness, typically by increasing egg production or longevity. in the present study, ovigeny index (oi) and impact of female fitness, as well as physiological state on the reproductive strategies, were investigated in the synovigenic parasitoid, diglyphus isaea, fed on host food (2-3rd ins ...201121641912
species displacements are common to two invasive species of leafminer fly in china, japan, and the united states.under field conditions, species displacements have occurred in different directions between the same invasive species of leafminers (diptera: agromyzidae). liriomyza sativae (blanchard) was displaced by l. trifolii (burgess) in the western united states, with evidence suggesting that lower insecticide susceptibility of l. trifolii is a factor. however, in japan, the opposite has occurred, as l. trifolii was recently displaced by l. sativae. this displacement is probably because of the higher fec ...201122299335
the complete mitochondrial genome of the leafminer liriomyza sativae (diptera: agromyzidae): great difference in the a+t-rich region compared to liriomyza trifolii.the complete mitochondrial genome sequence of liriomyza sativae blanchard (15,551bp) was determined and analyzed in this study. the circular genome contained 37 genes including 13 protein-coding genes, 22 trna genes, 2 rrna genes and an a+t-rich region. the initiation codons of coi and nd1 were 'atca' and 'gtg', respectively. nd2 gene used the truncated termination codon 't'. all the trna genes had the typical cloverleaf secondary structures except for trna(ser(agn)) gene, which was found with t ...201121703334
[antifeedant and antioviposition activities of momordica charantia leaf ethanol extract against liriomyza sativae].liriomyza sativae blanchard (diptera: agromyzidae) is one of the important pests harming a wide variety of vegetables and ornamental plants throughout the world. the leaf ethanol extract of momordica charantia at the concentration of 2000-4000 microg x ml(-1) displayed significant antifeedant and antioviposition activities against l. sativae adults. for further purifying the extract, four solvents, i. e., cyclohexane, ethyl acetate, n-butanol and water, were used to extract the ethanol extract, ...200919565764
cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer, liriomyza sativa.the vegetable leafminer, liriomyza sativae has spread worldwide, causing serious loss of agricultural productivity. heat shock proteins (hsps) play important roles in the environmental adaptation of various organisms, and to explore the functions of hsps in relation to cold tolerance and development in l. sativae, three full-length cdnas of small heat shock protein genes (ls-hsp19.5, ls-hsp20.8 and ls-hsp21.7) and two partial cdnas of tcp1 (the hsp60 homolog, ls-tcp1alpha and ls-tcp1zeta) were c ...200919133268
can greenhouses eliminate the development of cold resistance of the leafminers?latitudinal patterns for quantitative traits in insect are commonly used to investigate climatic adaptation. we compare the cold resistance of the leafminer (liriomyza sativae) pupa among populations distributed from tropical to temperate regions, incorporating the thermal overwintering limit of the insect's range. the patterns of cold resistance for northern and southern populations differ. the southern populations significantly increased their cold resistance with latitude, showing a latitudin ...200515800738
[suppressive effect of plant secondary substances and ecological measures on liriomyza sativae population].a state-space population model was used to evaluate the effect of plant secondary substances and ecological measures on the population dynamics of the vegetable leafminer, liriomyza sativae. in the vegetable field, by spraying alcohol extracts of lantana camara (1 g.100 ml-1), or spraying the petroleum spray oil (300 x), or releasing the parasitoids, the indices of population control(iipc) of l. sativae compared with control were 0.136, 0.222 and 0.292, respectively, but the index of population ...200314997652
genetic covariance between oviposition preference and larval performance in an insect experimental study determined that females of the herbivorous fly species liriomyza sativae (diptera: agromyzidae) preferentially oviposit on the plant species on which their female progeny attain the greatest pupal weight. a modified parent/offspring regression was used to quantify this relationship as an additive genetic covariance between host-plant preference and relative performance of female larvae on different plant species. the implications of a genetic covariance between preference a ...198628556150
host plant-related parasitism and host feeding activities of diglyphus isaea (hymenoptera: eulophidae) on liriomyza huidobrensis, liriomyza sativae, and liriomyza trifolii (diptera: agromyzidae).host plant species can affect the behavior and attributes of parasitoids, such as host searching, oviposition, and offspring fitness. in this study, parasitism, host feeding, and sex ratios of diglyphus isaea (walker) (hymenoptera: eulophidae) on liriomyza huidobrensis (blanchard), liriomyza sativae blanchard, and liriomyza trifolii (burgess) (diptera: agromyzidae) larvae reared on phaseolus vulgaris l., pisum sativum l., solanum lycopersicum l., and vicia faba l. were determined. in no-choice t ...201222420268
partitioning yield loss on yellow squash into nematode and insect components.the effect of a contplex of several insect and nematode pests on yield of yellow squash (cucurbita pepo l.) was examined in two field tests in southern florida. applications of permethrin for insect control and oxamyl primarily for nematode control plus some insect control were made alone and in combination to achieve differential reduction of various insect and nematode components contributing to yield loss. the effect of these components on yield was further analyzed by multiple regression. yi ...198219295683
[effect of temperature and relative-humidity on the development of liriomyza sativae blanchard (diptera: agromyzidae) in vigna unguiculata].this research aimed to study the influence of temperature and relative-humidity (rh) on the development of liriomyza sativae blanchard during the egg-adult period, in cowpea, to provide essential information for future biological control projects against the pest. an inverse relation was observed between temperature increase in the range from 15 degrees celsius to 32 degrees celsius and development duration. larval survival was not affected in the temperature range studied, while a high mortalit ...201020098917
reproductive activity and survivorship of liriomyza sativae (diptera: agromyzidae) at different temperatures and relative humidity levels.we studied a population of liriomyza sativae blanchard (diptera: agromyzidae) identified by morphological and molecular techniques from the semiarid region of the brazilian northeast. the influence of temperature and relative humidity on the survival and reproductive parameters of l. sativae in cowpeas (vigna unguiculata l. walp.) (fabales: fabaceae) was evaluated. we used temperatures of 18, 20, 22, 25, 28, 30, and 32 +/- 1 degrees c (50 +/- 10% rh) and relative humidity values of 30, 50, 70, a ...201020146857
primer design for identifying economically important liriomyza species (diptera: agromyzidae) by multiplex pcr.leafminer flies, especially, liriomyza huidobrensis, liriomyza sativae and liriomyza trifolii, are quarantine species in many countries. their morphological similarity makes identification difficult. to develop a rapid, reliable, sensitive and simple molecular identification method using multiplex pcr, we newly sequenced the mitochondrial cytochrome oxidase i (coi) genes of liriomyza bryoniae, liriomyza chinensis, l. huidobrensis, l. sativae, l. trifolii, chromatomyia horticola and four parasito ...201323039969
permanent genetic resources added to molecular ecology resources database 1 february 2013-31 march 2013.this article documents the addition of 142 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: agriophyllum squarrosum, amazilia cyanocephala, batillaria attramentaria, fungal strain ctey1 (ascomycota), gadopsis marmoratus, juniperus phoenicea subsp. turbinata, liriomyza sativae, lupinus polyphyllus, metschnikowia reukaufii, puccinia striiformis and xylocopa grisescens. these loci were cross-tested on the following species: amazi ...201323693143
Displaying items 1 - 40 of 40