Publications

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spinosad, a new tool for insect control in vegetables cultivated in greenhouses.spinosad is a biological insecticide derived as a fermentation product from the soil actinomycete saccharopolyspora spinosa. the compound was tested for its possible use in northwestern europe in vegetables cultivated in greenhouses. spinosad is an insect control agent that has activity against a wide range of insect pests including caterpillars, leafminers and thrips. because of its selectivity on a large number of beneficials used in greenhouses, spinosad is an interesting tool for insect cont ...200112425058
towards environmentally and human friendly insect pest control technologies: photosensitization of leafminer flies liriomyza bryoniae.development of new, ecologically safe technologies to control insect pest populations is of great importance. photoactive compounds usually used for photosensitization might be effective as pesticide agents, with low impact on the environment, being non-toxic and not mutagenic. phosensitizer accumulates within the insect body and, following exposure to visible light, induces lethal photochemical reactions and death. the aim of this study is to evaluate the possible usage of several photosensitiz ...200717766141
bacillus amyloliquefaciens ag1 biosurfactant: putative receptor diversity and histopathological effects on tuta absoluta midgut.the use of biosurfactant in pest management has received much attention for the control of plant pathogens, but few studies reported their insecticidal activity. the present study describes the insecticidal activity of biosurfactant extracted from bacillus amyloliquefaciens strain ag1. this strain produces a lipopeptide biosurfactant exhibiting an lc50 of about 180ng/cm(2) against tuta absoluta larvae. accordingly, the histopathologic effect of this biosurfactant on t. absoluta larvae showed ser ...201526299754
investigation of larvae digestive β-glucosidase and proteases of the tomato pest tuta absoluta for inhibiting the insect development.the tomato leaf miner tuta absoluta is one of the most devastating pests for tomato crops. digestive proteases and β-glucosidase enzymes were investigated using general and specific substrates and inhibitors. maximal β-glucosidase and proteolytic activities occurred at temperature and ph optima of 30 and 40°c, 5 and 10-11 unit of ph, respectively. zymogram analysis showed the presence of distinguished β-glucosidase exhibiting a specific activity of about 183 ± 15 µmol min-1 mg-1. in vitro inhibi ...201626898349
efficacy of entomopathogenic nematodes against larvae of tuta absoluta in the laboratory.previous studies have indicated the control potential of entomopathogenic nematodes (epns) against tuta absoluta. here, the potential of steinernema feltiae, s. carpocapsae and heterorhabditis bacteriophora is studied when applied against larvae of t. absoluta inside leaf mines in tomato leaf discs by means of an automated spray boom.201626620187
permanent genetic resources added to molecular ecology resources database 1 february 2013-31 march 2013.this article documents the addition of 142 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: agriophyllum squarrosum, amazilia cyanocephala, batillaria attramentaria, fungal strain ctey1 (ascomycota), gadopsis marmoratus, juniperus phoenicea subsp. turbinata, liriomyza sativae, lupinus polyphyllus, metschnikowia reukaufii, puccinia striiformis and xylocopa grisescens. these loci were cross-tested on the following species: amazi ...201323693143
primer design for identifying economically important liriomyza species (diptera: agromyzidae) by multiplex pcr.leafminer flies, especially, liriomyza huidobrensis, liriomyza sativae and liriomyza trifolii, are quarantine species in many countries. their morphological similarity makes identification difficult. to develop a rapid, reliable, sensitive and simple molecular identification method using multiplex pcr, we newly sequenced the mitochondrial cytochrome oxidase i (coi) genes of liriomyza bryoniae, liriomyza chinensis, l. huidobrensis, l. sativae, l. trifolii, chromatomyia horticola and four parasito ...201323039969
polymerase chain reaction-restriction fragment length polymorphism assays to distinguish liriomyza huidobrensis (diptera: agromyzidae) from associated species on lettuce cropping systems in italy.the pea leafminer, liriomyza huidobrensis (blanchard) (diptera: agromyzidae), is a serious insect pest infesting open field lettuce plantings in northern italy. in these cropping systems, it coexists with several other agromyzid species that have negligible economic importance on open field vegetables. the rapid detection of l. huidobrensis is crucial for effective management strategies, but the identification of agromyzids to species can be very difficult at adult as well at immature stages. in ...200616937681
increased water salinity applied to tomato plants accelerates the development of the leaf miner tuta absoluta through bottom-up effects.variation in resource inputs to plants may trigger bottom-up effects on herbivorous insects. we examined the effects of water input: optimal water vs. limited water; water salinity: with vs. without addition of 100 mm nacl; and their interactions on tomato plants (solanum lycopersicum), and consequently, the bottom-up effects on the tomato leaf miner, tuta absoluta (meytick) (lepidoptera: gelechiidae). plant growth was significantly impeded by limited water input and nacl addition. in terms of l ...201627619473
effect of two solanaceous plants on developmental and population parameters of the tomato leaf miner, tuta absoluta (meyrick) (lepidoptera: gelechiidae).tuta absoluta (meyrick) is an important tomato pest that also feeds on other host-plants from the solanceae family. we studied the effect of two cultivated plants, tomato (lycopersicum esculentum mill.) and potato solanum tuberosum l. on the development and populational parameters of t. absoluta related with host-plant suitability. larval developmental time, pupal weight, mean fecundity and an index of host-plant quality (ipq = pupal weight / frass weight) were estimated. age-specific survivorsh ...200617144141
new insight into the management of the tomato leaf miner, tuta absoluta (lepidoptera: gelechiidae) with entomopathogenic nematodes.the tomato leaf miner, tuta absoluta (meyrick) (lepidoptera: gelechiidae), is a serious threat to tomato production in the world. due to serious issues with insecticide resistance, there is a dire need for alternative control methods. entomopathogenic nematodes (epn) have potential for the biological control of t. absoluta. in the laboratory, we examined the effect of temperature, soil type, and exposure time on the efficacy of the epn species steinernema carpocapsae (nematoda: steinernematidae) ...201729272433
assessing the spatial distribution of tuta absoluta (lepidoptera: gelechiidae) eggs in open-field tomato cultivation through geostatistical analysis.the spatial distribution of insects is due to the interaction between individuals and the environment. knowledge about the within-field pattern of spatial distribution of a pest is critical to planning control tactics, developing efficient sampling plans, and predicting pest damage. the leaf miner tuta absoluta (meyrick) (lepidoptera: gelechiidae) is the main pest of tomato crops in several regions of the world. despite the importance of this pest, the pattern of spatial distribution of t. absol ...201828714108
humic fertilizer and vermicompost applied to the soil can positively affect population growth parameters of trichogramma brassicae (hymenoptera: trichogrammatidae) on eggs of tuta absoluta (lepidoptera: gelechiidae).the tomato leaf miner, tuta absoluta (meyrick), is a devastating pest of tomato worldwide. one of the control measures of t. absoluta is the use of biological control agents, such as trichogramma wasps. interactions between natural enemies and insect pests may be affected by application of fertilizers, because changes in plant quality through the fertilizer application may therefore affect herbivore characteristics and suitability of them to parasitism. laboratory tests were carried out to evalu ...201728664527
estimating the development rate of the tomato leaf miner, tuta absoluta (lepidoptera: gelechiidae), using linear and non-linear models.tuta absoluta meyrick (lepidoptera: gelechiidae) is native to south america and has recently invaded european, african and asian countries, where it is causing severe damage to tomato crops leading to an increase in the number of insecticide applications. this situation has prompted a demand for alternative pest management strategies aiming to control t. absoluta and concomitantly reduce insecticide applications. the development period for immature stages of t. absoluta at constant temperatures ...201727860137
insecticide resistance mediated by an exon skipping event.many genes increase coding capacity by alternate exon usage. the gene encoding the insect nicotinic acetylcholine receptor (nachr) α6 subunit, target of the bio-insecticide spinosad, is one example of this and expands protein diversity via alternative splicing of mutually exclusive exons. here, we show that spinosad resistance in the tomato leaf miner, tuta absoluta is associated with aberrant regulation of splicing of taα6 resulting in a novel form of insecticide resistance mediated by exon ski ...201627748560
life table parameters of tuta absoluta (lepidoptera: gelechiidae) on different varieties of tomato.tuta absoluta (meyrick), a tomato leaf miner, is one of the destructive pests of tomatoes, which is native to south america, and has been considered as a quarantined pest for iran since 2010 while it has quickly spread all around the country and is known as a key pest in tomato-cultured regions since 2012. in the current study, the life table parameters of t. absoluta were studied on cut leaves of three greenhouse cultivars of tomato including 'atabay', 'cluse', and 'perenses'. data were analyze ...201426309265
physiological selectivity and activity reduction of insecticides by rainfall to predatory wasps of tuta absoluta.in this study, we carried out three bioassays with nine used insecticides in tomato crops to identify their efficiency against tomato leaf miner tuta absoluta, the physiological selectivity and the activity reduction of insecticides by three rain regimes to predatory wasps protonectarina sylveirae and polybia scutellaris. we assessed the mortality caused by the recommended doses of abamectin, beta-cyfluthrin, cartap, chlorfenapyr, etofenprox, methamidophos, permethrin, phenthoate and spinosad to ...201525421627
susceptibility of tuta absoluta (lepidoptera: gelechiidae) brazilian populations to ryanodine receptor modulators.phthalic and anthranilic diamides comprise a new insecticide class recently registered in brazil to control lepidoptera such as tuta absoluta (meyrick). therefore, the baseline of susceptibility was determined for eight representative field populations of this species to establish a resistance monitoring programme. the potential for cross-resistance as well as detoxification metabolism was assessed in order to fine-tune the resistance management programme.201524863675
development of polymorphic microsatellite loci for the tomato leaf miner, tuta absoluta (lepidoptera: gelechiidae). 201324346326
analysis of three leafminers' complete mitochondrial genomes.liriomyza trifolii (burgess), liriomyza huidobrensis (blanchard), and liriomyza bryoniae (kaltenbach), are three closely related and economically important leafminer pests in the world. this study examined the complete mitochondrial genomes of l. trifolii, l. huidobrensis and l. bryoniae, which were 16,141 bp, 16,236 bp and 16,183 bp in length, respectively. all of them displayed 37 typical animal mitochondrial genes and an a+t-rich region. the genomes were highly compact with only 60-68 bp of n ...201323954222
identification of mutations associated with pyrethroid resistance in the voltage-gated sodium channel of the tomato leaf miner (tuta absoluta).the tomato leaf miner, tuta absoluta (lepidoptera) is a significant pest of tomatoes that has undergone a rapid expansion in its range during the past six years and is now present across europe, north africa and parts of asia. one of the main means of controlling this pest is through the use of chemical insecticides. in the current study insecticide bioassays were used to determine the susceptibility of five t. absoluta strains established from field collections from europe and brazil to pyrethr ...201222504519
kairomone attractant for the leafmining fly, liriomyza bryoniae (diptera, agromyzidae).a field test carried out in an industrial greenhouse in lithuania revealed the attractiveness of synthetic methyl salicylate (mesa) towards an economically important leafmining tomato pest, liriomyza bryoniae (kaltenbach) (diptera, agromyzidae). the behavioural reaction of the flies depended very much on the simultaneous presence of both olfactory and visual stimuli. the attractiveness depended on the colour of a sticky trap: mesa attracted significantly more flies (ca. 2.2 times) when placed in ...201618811011
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