| [new data on the ixodid tick fauna of the western pamirs]. | dermacentor raskemensis and haemaphysalis pospelovashtromae were first recorded from the west pamirs in the flood-land of the gunt river at the height of 2700 m above the sea level. the ticks were collected from sheep, on which in addition to the above species d. marginatus was found. in the rushansk region of the mountain-badakhshan district at the height of 2100 to 2300 m rhipicephalus pumilio, r. turanicus and hyalomma plumbeum were collected from domestic sheep and goats. | 1986 | 2950368 |
| astrakhan fever rickettsiae: antigenic and genotypic analysis of isolates obtained from human and rhipicephalus pumilio ticks. | two spotted fever group rickettsia strains, a-108 and a-167, were isolated from the hemolymph of rhipicephalus pumilio ticks collected in the astrakhan region of russia, which is area endemic for astrakhan fever. these tick isolates were compared with a strain isolated from a patient suffering from astrakhan fever and with reference spotted fever group rickettsiae strains. new tick isolates and the human strain were identical in their serologic, antigenic, and genetic characteristics by several ... | 1994 | 7985764 |
| [the primary effect and characteristics of the course of a new tick-borne rickettsiosis--astrakhan fever]. | clinicomorphological characteristics are given of primary reaction at the site of tick (rhipicephalus pumilio) bite in new rickettsiosis spreading in astrakhan province--astrakhan fever. the disease presented with long-term fever (body temperature 39-40 degrees c), intoxication, conjunctivitis, hepatomegaly, regional lymphadenitis, disseminated roseolous papular-hemorrhagic rash, arterial hypotension. | 1996 | 9324803 |
| taxonomic relationships among spotted fever group rickettsiae as revealed by antigenic analysis with monoclonal antibodies. | the spotted fever group (sfg) is made up of more than 20 different rickettsial species and strains. study of the taxonomic relationships among the group has been attempted by phenotypic, genotypic, and phylogenetic analyses. in this study, we determined taxonomic relationships among the sfg rickettsiae by comparative analysis of immunogenic epitopes reactive against a panel of monoclonal antibodies. a total of 98 monoclonal antibodies, which were directed against epitopes on the major immunodomi ... | 1998 | 9542904 |
| phylogenetic placement of rickettsiae from the ticks amblyomma americanum and ixodes scapularis. | a rickettsial isolate (isolate moaa) belonging to the spotted fever group (sfg) was obtained from the lone star tick amblyomma americanum. we used pcr to characterize the genes for the rickettsial outer membrane proteins rompa and rompb. we sequenced the pcr products (domains i of both the rompa gene and the rompb gene) of moaa and wb-8-2, another rickettsial isolate from a. americanum. to place moaa and wb-8-2 and two other nonpathogenic isolates (rickettsia rickettsii hip2 and rickettsia monta ... | 1998 | 9574696 |
| in vitro susceptibilities of 27 rickettsiae to 13 antimicrobials. | the mics of 13 antibiotics (doxycycline, thiamphenicol, rifampin, amoxicillin, gentamicin, co-trimoxazole, ciprofloxacin, pefloxacin, ofloxacin, erythromycin, josamycin, clarithromycin, and pristinamycin) were determined for 27 available rickettsial species or strains. we used two in vitro cell culture methods described previously: the plaque assay and the microplaque colorimetric assay. our results confirm the susceptibilities of rickettsiae to doxycycline, thiamphenicol, and fluoroquinolones. ... | 1998 | 9660979 |
| distinguishing species and populations of rhipicephaline ticks with its 2 ribosomal rna. | most populations and some species of ticks of the genera boophilus (5 spp.) and rhipicephalus (ca. 75 spp.) cannot be distinguished phenotypically. moreover, there is doubt about the validity of species in these genera. i studied the entire second internal transcribed spacer (its 2) rrna of 16 populations of rhipicephaline ticks to address these problems: boophilus microplus from australia, kenya, south africa and brazil (4 populations); boophilus decoloratus from kenya; rhipicephalus appendicul ... | 1998 | 9794625 |
| [new and emerging rickettsial and bartonella infections]. | there have been recently reports on over 10 new and resurgent rickettsioses and bartonelloses in different countries, which reflects both socioeconomic processes in society and a higher methodological level of indication and identification of causative agents. in 1991, the author' laboratory, n. f. gamaleya research institute of experimental medicine, russian academy of medical sciences, established the etiology of the new rickettsiosis astrakhan spotted fever. it separated and studied 2 strains ... | 2001 | 11837209 |
| detection of ehrlichia spp., anaplasma spp., rickettsia spp., and other eubacteria in ticks from the thai-myanmar border and vietnam. | a total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the thai-myanmar border and in vietnam. they were tested by pcr to detect dna of bacteria of the order rickettsiales: three anaplasma spp. were detected in ticks collected in thailand, including (i) anaplasma sp. strain anda465, which was considered a genotype of anaplasma platys (formerly ehrlichia platys) and which was obtained from dermacentor aur ... | 2003 | 12682151 |
| detection of astrakhan fever rickettsia from ticks in kosovo. | astrakhan fever is a summer spotted fever resembling mediterranean spotted fever, endemic in astrakhan, a region of russia located by the caspian sea. its agent is a spotted fever group rickettsia, member of the rickettsia conorii complex, transmitted to humans by rhipicephalus sanguineus and rhipicephalus pumilio ticks. in summer 2001, french united nations troops in kosovo collected 2 ticks on asymptomatic soldiers (1 r. sanguineus and 1 hyalomma marginatum) and 10 ticks on dogs (7 r. sanguine ... | 2003 | 12860620 |
| detection of a rickettsia closely related to rickettsia aeschlimannii, "rickettsia heilongjiangensis," rickettsia sp. strain rpa4, and ehrlichia muris in ticks collected in russia and kazakhstan. | using pcr, we screened 411 ticks from four genera collected in russia and kazakhstan for the presence of rickettsiae and ehrlichiae. in russia, we detected "rickettsia heilongjiangensis," rickettsia sp. strain rpa4, and ehrlichia muris. in kazakhstan, we detected rickettsia sp. strain rpa4 and a rickettsia closely related to rickettsia aeschlimannii. these agents should be considered in a differential diagnosis of tick-borne infections in these areas. | 2004 | 15131195 |
| proposal to create subspecies of rickettsia conorii based on multi-locus sequence typing and an emended description of rickettsia conorii. | rickettsiae closely related to the malish strain, the reference rickettsia conorii strain, include indian tick typhus rickettsia (ittr), israeli spotted fever rickettsia (isfr), and astrakhan fever rickettsia (afr). although closely related genotypically, they are distinct serotypically. using multilocus sequence typing (mlst), we have recently found that distinct serotypes may not always represent distinct species within the rickettsia genus. we investigated the possibility of classifying ricke ... | 2005 | 15766388 |
| tick-borne rickettsioses around the world: emerging diseases challenging old concepts. | during most of the 20th century, the epidemiology of tick-borne rickettsioses could be summarized as the occurrence of a single pathogenic rickettsia on each continent. an element of this paradigm suggested that the many other characterized and noncharacterized rickettsiae isolated from ticks were not pathogenic to humans. in this context, it was considered that relatively few tick-borne rickettsiae caused human disease. this concept was modified extensively from 1984 through 2005 by the identif ... | 2005 | 16223955 |
| ticks of small ruminants in china. | the importance of ticks and tick-borne diseases of small ruminants in china is discussed. of the 109 species of ticks identified to date in china, 45 species infest small ruminants. five species have been proved to be involved, or possibly involved, in the transmission of tick-borne diseases. anaplasma ovis, babesia motasi, babesia ovis and two unidentified species of theileria, have been recorded in small ruminants in china. the diseases caused by these organisms are widespread in china, causin ... | 2007 | 17823826 |
| [detection of tick and tick-borne pathogen in some ports of inner mongolia]. | to investigate the distribution, composition and situation of natural infection pathogen of tick species in the main ports of inner mongolia. | 2009 | 19731529 |
| development and evaluation of a loop-mediated isothermal amplification method for rapid detection of anaplasma ovis. | anaplasma ovis is an intraerythrocytic rickettsial pathogen of small ruminants. loop-mediated isothermal amplification (lamp) is a nucleic acid detection method in which the target dna can be efficiently amplified with high specificity and sensitivity under isothermal conditions. in this study, a lamp method was developed for the specific detection of a. ovis, using lamp primers designed on the basis of the major surface protein 4 gene. lamp was performed at 65°c for 30 min. its specificity was ... | 2011 | 21471346 |
| tick cell culture isolation and growth of rickettsia raoultii from dutch dermacentor reticulatus ticks. | tick cell lines play an important role in research on ticks and tick-borne pathogenic and symbiotic microorganisms. in an attempt to derive continuous dermacentor reticulatus cell lines, embryo-derived primary cell cultures were set up from eggs laid by field ticks originally collected as unfed adults in the netherlands and maintained for up to 16 months. after several months, it became evident that cells in the primary cultures were infected with a rickettsia-like intracellular organism. supern ... | 2012 | 23140894 |
| prevalence and burden of two rickettsial phylotypes (g021 and g022) in ixodes pacificus from california by real-time quantitative pcr. | the western black-legged tick, ixodes pacificus cooley and kohls, commonly bites humans in the far western u.s. in addition to transmitting lyme borreliosis and anaplasmosis, it is a host of nonpathogenic bacteria as well as some of unknown pathogenicity. in this study, we report the detection, prevalence, and burden of 2 rickettsial phylotypes with unknown pathogenicity in i. pacificus ticks from 6 california counties using real-time quantitative pcr with phylotype-specific primers and probes. ... | 2013 | 23522936 |
| emerging tick-borne infections in mainland china: an increasing public health threat. | since the beginning of the 1980s, 33 emerging tick-borne agents have been identified in mainland china, including eight species of spotted fever group rickettsiae, seven species in the family anaplasmataceae, six genospecies in the complex borrelia burgdorferi sensu lato, 11 species of babesia, and the virus causing severe fever with thrombocytopenia syndrome. in this review we have mapped the geographical distributions of human cases of infection. 15 of the 33 emerging tick-borne agents have be ... | 2015 | 26453241 |
| pathogens vectored by the tick, dermacentor reticulatus, in endemic regions and zones of expansion in poland. | dermacentor reticulatus plays an important role in the maintenance of pathogens of medical and veterinary importance in the environment. currently two isolated populations of d. reticulatus are present in poland--western and eastern. the range of the eastern population covers endemic areas in eastern poland but this population is expanding westwards creating an expansion zone in the centre of the country. the expansion zone in western poland is occupied by the recently discovered western populat ... | 2015 | 26403456 |
| tick-borne infections in human and animal population worldwide. | the abundance and activity of ectoparasites and its hosts are affected by various abiotic factors, such as climate and other organisms (predators, pathogens and competitors) presenting thus multiples forms of association (obligate to facultative, permanent to intermittent and superficial to subcutaneous) developed during long co-evolving processes. ticks are ectoparasites widespread globally and its eco epidemiology are closely related to the environmental conditions. they are obligatory hematop ... | 2015 | 27047089 |
| human infections with rickettsia raoultii, china. | we used molecular methods to identify rickettsia raoultii infections in 2 persons in china. these persons had localized rashes around sites of tick bites. r. raoultii dna was detected in 4% of dermacentor silvarum ticks collected in the same area of china and in 1 feeding tick detached from 1 patient. | 2014 | 24750663 |
| molecular detection of anaplasma spp. and ehrlichia spp. in ruminants from twelve provinces of china. | anaplasma spp. and ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. there is little information on anaplasmosis and ehrlichiosis in ruminants in china. 16s rrna fret-qpcrs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of china for anaplasma spp. and ehrlichia spp. positive samples were further analyzed with a standard pcr for the glta. anaplasm ... | 2016 | 28096822 |
| genome sequence of the tick-borne pathogen rickettsia raoultii. | italic! rickettsia raoultiiis a tick-associated spotted fever group (sfg) organism, causing scalp eschar and neck lymphadenopathy after tick bite (senlat) in humans. we report here the genome sequence of italic! r. raoultiistrain khabarovsk(t)(csur r3(t), atcc vr-1596(t)), which was isolated from a italic! dermacentor silvarumtick collected in russia. | 2016 | 27103706 |
| update on tick-borne rickettsioses around the world: a geographic approach. | tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ... | 0 | 24092850 |
| rickettsia raoultii in haemaphysalis erinacei from marbled polecats, china-kazakhstan border. | we found rickettsia raoultii dna in 2 out of 32 (6.25 %) haemaphysalis erinacei ticks. result showed that the sequences of five genes (17-kda, glta, ompa, rrs, and ompb) were 100 % identity with that of r. raoultii in genbank. this study is the first report on the presence of r. raoultii in h. erinacei from wild marbled polecat, vormela peregusna. our findings suggest that h. erinacei parasitizing wild marbled polecat may serve as reservoir and carriers for r. raoultii in areas around the china- ... | 2015 | 26383238 |
| biosurveillance in central asia: successes and challenges of tick-borne disease research in kazakhstan and kyrgyzstan. | central asia is a vast geographic region that includes five former soviet union republics: kazakhstan, kyrgyzstan, tajikistan, turkmenistan, and uzbekistan. the region has a unique infectious disease burden, and a history that includes silk road trade routes and networks that were part of the anti-plague and biowarfare programs in the former soviet union. post-soviet union biosurveillance research in this unique area of the world has met with several challenges, including lack of funding and res ... | 2016 | 26870722 |
| morphological and genetic diversity of rhipicephalus sanguineus sensu lato from the new and old worlds. | the taxonomic status of the brown dog tick (rhipicephalus sanguineus sensu stricto), which has long been regarded as the most widespread tick worldwide and a vector of many pathogens to dogs and humans, is currently under dispute. | 2013 | 23880226 |
| genome sequence of rickettsia conorii subsp. caspia, the agent of astrakhan fever. | rickettsia conorii subsp. caspia is the agent of astrakhan fever, a spotted fever group rickettsiosis endemic to astrakhan, russia. the present study reports the draft genome of rickettsia conorii subsp. caspia strain a-167. | 0 | 22887666 |
| a secondary structural common core in the ribosomal its2 (internal transcribed spacer) of culexspecies from diverse geographical locations. | in the present study, sequence and structural analysis of its2 region (the spacer segment between 5.8s and 28s rrna of mature rrna sequences) of 7 culex species belonging to 5 different geographical locations was carried out. alignment of the its2 sequence from the 7 species revealed 8 homologous domains. four species namely c. vishnui, c. annulus, c. pipiens, c. quiquefasciatusshowed high sequence (98-100%) and rna secondary structure similarity. the its2 similarity among different species is h ... | 2005 | 17597853 |
| isolation and maintenance of rickettsia raoultii in a rhipicephalus sanguineus tick cell line. | rickettsia raoultii, a member of the spotted fever group rickettsiae, has been implicated in cases of debonel/tibola/senlat, and has been detected in dermacentor spp. and rhipicephalus pumilio ticks by pcr. r. raoultii has been isolated in mammalian and tick cell lines. this study aimed to isolate r. raoultii from spanish dermacentor marginatus in tick cell lines. a single adult d. marginatus collected from vegetation in la rioja (northen spain) in october 2012 was surface-sterilised, triturated ... | 2016 | 26428859 |