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[the virus carriage of ticks, vectors of the causative agent of crimean hemorrhagic fever].the study of ixodes ticks collected in astrakhan province in 1982-1987 has revealed spontaneous infection with crimean hemorrhagic fever virus in 4 species. it has been established that a proportion of the infected ck suspensions from the imago hyalomma marginatum marginatum (a back-ground species for the given region) was 3.6% as measured by the method of mouse infection and 8.9% as measured by the enzyme immunoassay. the advantages of the enzyme immunoassay as a method for crimean hemorrhagic ...19911829784
[indication of the antigen to crimean-congo hemorrhagic fever in vector ticks using a solid-phase immunoenzyme technic].paper presents the results of a successful use of a solid-phase immunoassay for indication of the antigen of hemorrhagic fever-crimean--congo virus in lays and half-fed female-ticks hyalomma marginatum marginatum koch (1795) collected in endemic area, which adds to our knowledge of this disease focus activity.19892496286
prevalence of rickettsia-like organisms and spotted fever group rickettsiae in ticks (acari: ixodidae) from zimbabwe.the prevalence of rickettsia-like organisms in ticks from zimbabwe was determined using the hemolymph test. amblyomma hebraeum had the highest prevalence of rickettsia-like organisms. other species with rickettsia-like organisms included amblyomma sparsum, amblyomma variegatum, hyalomma marginatum rufipes, ripicephalus simus, haemaphysalis leachi, amblyomma rhinocerotis, and hyalomma truncatum. ticks with no demonstrable rickettsia-like organisms infection were boophilus decoloratus, haemaphysal ...19958551500
ticks of domestic animals in the macedonia region of greece.a study was carried out on ticks of domestic animals in the macedonia region of greece. during 1983-1986, 11,620 tick specimens, belonging to 18 species and subspecies, were collected from cattle, sheep, goats and dogs, in 64 localities throughout macedonia. rhipicephalus bursa, the most common tick, and hyalomma marginatum marginatum occurred in all bioclimatic zones, as well as the two one-host ticks, boophilus annulatus and h. detritum scupense, which were present in fewer localities. r. tura ...19968792578
rickettsia aeschlimannii sp. nov., a new spotted fever group rickettsia associated with hyalomma marginatum ticks.we formally propose the name rickettsia aeschlimannii sp. nov. for a new spotted fever group (sfg) rickettsia, strain mc16t, isolated from hyalomma marginatum marginatum ticks collected in morocco. this organism shows a typical rickettsial morphology when analyzed by electron microscopy. after characterization by serotyping, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western immunoblotting, pcr-restriction fragment length polymorphism (rflp), pulsed-field gel electrophoresis, and ...19979103647
comparative immunizing power of infections, salivary extracts, and intestinal extracts of hyalomma marginatum marginatum in cattle.tick concealed antigens have been successful in producing immunity that inhibits tick fertility, but require periodic revaccination and are little effective in preventing tick feeding, which is critical to stop pathogen transmission. tick natural salivary antigens also induce important immunity, but revaccination may be unnecessary in enzootic areas. in addition these antigens may inhibit tick feeding. we immunized groups of three tick-naive calves with four prior infestations with hyalomma marg ...19979106957
comparison of effects of low and high tick infestations on acquired cattle tick resistance: hyalomma marginatum marginatum.three holstein calves were infested with low numbers of ticks, two or three pairs of adults hyalomma marginatum marginatum in cloth bag daily for 21 days. infestation was carried out during tick proliferation periods. two months later, cattle leads were challenged with 100 pairs of ticks. another group of three holstein calves were infested five times with 100 pairs of adult ticks of the same species. the five infestations were performed two weeks from the previous infestation. three tick charac ...19989754299
molecular characterization of a haemaphysalis longicornis tick salivary gland-associated 29-kilodalton protein and its effect as a vaccine against tick infestation in rabbits.the use of tick vaccines in mammalian hosts has been shown to be the most promising alternative tick control method to current use of acaricides, which suffers from a number of limitations. however, the success of this method is dependent on the identification, cloning, and in vitro expression of tick molecules involved in the mediation of key physiological roles with respect to the biological success of a tick as a vector and pest. we have sequenced and characterized a haemaphysalis longicornis ...199910084999
sense organs in post-embryonic stages of hyalomma marginatum marginatum koch, 1844 (acari: ixodida: ixodidae). i. tarsal sensory system.light and scanning electron microscopic studies showed the differences in morphology and in size of haller's organ in larvae, nymphs and adults (females and males) of hyalomma marginatum marginatum koch, 1844. the length of the anterior pit setae increases during post-embryonic development. the localization of these setae is the same in all stages. six setae (one porose seta, two grooved setae, two fine setae, one conical seta) contain anterior pit of various developmental stages. in nymphs and ...199810376284
female tick hyalomma marginatum marginatum salivary glands: preliminary study on protein changes during feeding process and antigens recognized by repeatedly infested cattle.proteins extracted from salivary glands of unfed, three days and five days fed adult hyalomma marginatum marginatum were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). we have noticed changes during the three feeding steps. some proteins disappeared during feeding process (23, 38, 39, 40 to 50, 95 and 112 kda), they might be proteins which were converted in other substances and are secreted. other antigens (13 to 14, 20, 25, 29, 165 and 210 kda) were synthesize ...199910633500
distribution and ecology of ticks (acari: ixodidae) infesting livestock in tunisia: an overview of eighth years field collections.ticks (ixodidae) play a significant role as vectors of pathogens of domestic animals in tunisia. the major losses caused by ticks are related to transmission of protozoan parasites. these include agents of tropical theileriosis and babesiosis in ruminants. since 1991, we conducted research studies on tick population of livestock in tunisia. this overview reports a synthesis on tick distribution, their biology and their role as vectors of pathogens in domestic animals, particularly cattle. during ...199911071534
general review of tick species present in portugal.at present, 24 species are known to occur in portugal: argas vespertilionis, ornithodoros maritimus and ornithodoros erraticus in argasidae; ixodes acuminatus, ixodes bivari, ixodes canisuga, ixodes frontalis, ixodes hexagonus, ixodes ricinus, ixodes simplex simplex, ixodes ventalloi, ixodes vespertilionis, dermacentor marginatus, dermacentor pictus', haemaphysalis hispanica, haemaphysalis inermis, haemaphysalis punctata, rhipicephalus bursa, rhipicephalus pusillus, rhipicephalus sanguineus, rhi ...199911071535
epidemiology of mediterranean theileriosis in extremadura region, spain.extremadura is an extensive region (41,000 km2) located in the southwest of spain, where animal husbandry is a very important economical resource. livestock parasitism of ticks is common in the region and it is favoured by the environmental conditions and management systems. mediterranean theileriosis (mt) is perhaps the most important tick-borne disease of cattle. although theileria annulata has been identified in extremadura since 1933, little epidemiological information is available. during t ...199911071543
experimental teratogeny in the tick hyalomma marginatum marginatum (acari: ixodida: ixodidae): effect of high humidity on embryonic development.the effect of 90% rh on the embryonic development of hyalomma marginatum marginatum koch was investigated at 25 degrees c. under the influence of this factor, 2.1% dead eggs, 13.0% dead embryos, 6.9% abnormally hatched larvae, 0.2% larvae with malformations, and 77.8% normal larvae appeared. the embryos died during the cleavage of nuclei, the formation of the blastoderm, the formation of the germ band and its metamerization, and the differentiation of the leg anlagen. egg hatch was also inhibite ...200011126534
sense organs in post-embryonic stages of hyalomma marginatum marginatum koch, 1844 (acari: ixodida: ixodidae). ii. palpal organ.light microscopy and scanning electron microscopy (sem) studies show the differences in structure, number and topography of terminal setae in the palpal organ in different stages of hyalomma marginatum marginatum koch, 1844. the palpal organ contains eight terminal setae in larvae and nymphs, and ten setae in adults. the length of these setae increases in the course of post-embryonic development. other setae on palpal segments also undergo quantitative and morphological changes.200011686078
a comparison of susceptibilities to infection of four species of hyalomma ticks with theileria annulata.in this comparative study unfed nymphs of four hyalomma tick species (hyalomma anatolicum anatolicum, hyalomma anatolicum excavatum, hyalomma detritum and hyalomma marginatum marginatum) were allowed to engorge on calves experimentally infected with theileria annulata. the infection prevalence in the salivary glands of the adult female and male ticks of each hyalomma species used in the study were assessed. the infection prevalence with t. annulata was high and did not vary markedly in the four ...200312695036
ticks (ixodoidea) on birds migrating from africa to europe and asia.hyalomma marginatum marginatum of europe and asia and h. marginatum rufipes of africa, both reservoirs and vectors of organisms pathogenic to humans and animals, have not infrequently been found as incidental specimens or established populations far from their normal endemic geographical boundaries. owing to ticks' unique ability to transmit or harbour for long periods a variety of pathogens of man and animals, their potential epidemiological role is suspected wherever they occur. evidence that ...196113715709
studies on the epidemiology of tropical theileriosis (theileria annulata infection) in cattle in central anatolia, turkey.an epidemiological survey for theileria annulata infection was conducted in 12 selected villages around ankara in central anatolia, turkey, during the period april 1990 to january 1993. during the survey, 198 cattle of 30 local breeds, 84 holstein-friesian x local breeds and 84 holstein-friesian breed were examined for antibodies to t. annulata and the presence of the vector ticks. four species of hyalomma ticks were identified: hyalomma anatolicum anatolicum, hyalomma anatolicum excavtum, hyalo ...200314690090
cattle infestation by hyalomma ticks and prevalence of theileria in hyalomma species in the east of turkey.a total of 2388 cattle and 442 shelters, from two provinces (elazig and malatya) endemic for tropical theileriosis in the east of turkey, were studied for hyalomma tick populations from july 1993 to july 1995 in elazig and from may 1998 to january 1999 in malatya. four thousand five hundred and eighty one of 7455 hyalomma ticks were collected from cattle, the other ticks (2874) were collected from shelters. all of the ticks collected from shelters were hyalomma anatolicum anatolicum. two thousan ...200415036571
detection and identification of ehrlichia spp. in ticks collected in tunisia and morocco.a broad-range 16s rrna gene pcr assay followed by partial sequencing of the 16s rrna gene was used for the detection of members of the family anaplasmataceae in ticks in north africa. a total of 418 questing ixodes ricinus ticks collected in tunisia and morocco, as well as 188 rhipicephalus ticks from dogs and 52 hyalomma ticks from bovines in tunisia, were included in this study. of 324 adult i. ricinus ticks, 16.3% were positive for ehrlichia spp., whereas only 3.4 and 2.8% of nymphs and larva ...200515750072
tick-borne rickettsioses around the world: emerging diseases challenging old concepts.during most of the 20th century, the epidemiology of tick-borne rickettsioses could be summarized as the occurrence of a single pathogenic rickettsia on each continent. an element of this paradigm suggested that the many other characterized and noncharacterized rickettsiae isolated from ticks were not pathogenic to humans. in this context, it was considered that relatively few tick-borne rickettsiae caused human disease. this concept was modified extensively from 1984 through 2005 by the identif ...200516223955
prevalence of borrelia burgdorferi sensu lato in ticks collected from migratory birds in switzerland.the prevalence of ticks infected by borrelia burgdorferi sensu lato on birds during their migrations was studied in switzerland. a total of 1,270 birds captured at two sites were examined for tick infestation. ixodes ricinus was the dominant tick species. prevalences of tick infestation were 6% and 18.2% for birds migrating northward and southward, respectively. borrelia valaisiana was the species detected most frequently in ticks, followed by borrelia garinii and borrelia lusitaniae. among bird ...200616391149
ixodid ticks parasitizing iberian red deer (cervus elaphus hispanicus) and european wild boar (sus scrofa) from spain: geographical and temporal distribution.commercial hunting of spanish wild ungulates has made them an important economic resource. wild ungulates may have an important role in the maintenance of ixodid tick populations, and also as reservoirs of pathogens. we studied the ixodid ticks that parasitize iberian red deer and european wild boar from spain. ixodid ticks (n=6,336) were collected from 431 iberian red deer and 142 wild boar in different regions of spain. we found 10 different ixodid tick species parasitizing iberian red deer, m ...200616675125
[local anomalies in ticks larvae (acari: ixodida)].three anomalies in the anus structure of hyalomma marginatum marginatum larvae, two anomalies in argas reflexus larvae, i.e. the lack of the left chelicera and the second pair of limbs at the right side, and disorder in chetotaxy of argas persicus larvae were examined in this research. these anomalies have not been described so far in the literature.200416859039
crimean-congo hemorrhagic fever virus: genetic analysis and tick survey in turkey.crimean-congo hemorrhagic fever virus (cchfv) is a tick-borne virus in the family bunyaviridae, genus nairovirus. the virus is transmitted to humans through infected tick bites or from direct contact with viremic animals or humans. in the present study, a total of 1,015 adult ticks were collected from cattle (603 specimens), sheep (17 specimens), and goats (395 specimens) in the kelkit valley in turkey. four tick species were recognized on the animals in the surveyed region. the most abundant sp ...200617088370
detection of theileria and babesia species in ticks collected from cattle.the present study was carried out to detect tick species that infest cattle, and theileria and babesia species transmitted by these ticks in kayseri province (turkey). a total of 300 cattle were examined for tick infestations. of the 300 cattle, 117 (39%) were infested with ticks. a total of 1160 ticks belonging to 11 ixodid genera were collected from the infested animals and their shelters. the most prevalent tick species was boophilus annulatus 26.37% (306/1160) followed by hyalomma marginatum ...200717614205
detection of "rickettsia sp. strain uilenbergi" and "rickettsia sp. strain davousti" in amblyomma tholloni ticks from elephants in africa.to date, 6 tick-borne rickettsiae pathogenic for humans are known to occur in africa and 4 of them were first identified in ticks before being recognized as human pathogens.200717683629
detection of a questing hyalomma marginatum marginatum adult female (acari, ixodidae) in southern germany.the first finding of a questing hyalomma marginatum marginatum female tick in germany is described. the tick was found in may 2006 on the clothing of a person who had spent the preceding day in rural surroundings in southern germany. as the infested person had also been visiting spain where h. m. marginatum is known to occur some weeks prior to finding the tick, it is not clear whether the tick had been imported by him as a female or by another host in a preimaginal stage and succeeded to develo ...200717952610
genetic analysis of the m rna segment of crimean-congo hemorrhagic fever virus strains in turkey.crimean-congo hemorrhagic fever (cchf) virus is member of the genus nairovirus of the family bunyaviridae. all members of the family bunyaviridae are enveloped viruses containing tripartite, negative polarity, single-stranded rna. cchf is characterized by high case mortality, occurring in asia, africa, the middle east and europe. during recent years, outbreaks have been reported in turkey. however, little information is available on the genetic diversity of cchf virus in turkey. in this study, a ...200817955162
ticks infesting domestic animals in northern greece.the tick species infesting grazing animals in the countryside of 11 prefectures in northern greece were investigated during april-july and september-december of consecutive years 2003-2006. a total of 3,249 (1,952 males, 1,297 females) adult ticks were collected from goats, sheep, cattle and dogs. ticks were identified as ixodes ricinus (44.57%), ixodes gibbosus (4.09%), rhipicephalus bursa (19.14%), rhipicephalus sanguineus, rhipicephalus turanicus (5.79%), hyalomma marginatum marginatum (12.40 ...200818563592
spotted fever group rickettsiae in ticks, morocco.a total of 370 ticks, encompassing 7 species from 4 genera, were collected during 2002-2006 from domestic animals and vegetation in the taza region of northeastern morocco. rickettsial dna was identified in 101 ticks (27%) by sequencing pcr products of fragments of the citrate synthase and outer membrane protein genes of rickettsia spp. seven rickettsiae of the spotted fever group were identified, including 4 pathogens: r. aeschlimannii in hyalomma marginatum marginatum, r. massiliae in rhipicep ...200818598627
rickettsia aeschlimannii infection, algeria. 200818976583
prevalence and genotypes of anaplasma species and habitat suitability for ticks in a mediterranean ecosystem.anaplasma species are tick-transmitted pathogens that impact veterinary and human health. sicily is one of the locations where these pathogens are endemic. sicily represents a typical mediterranean ecosystem to study anaplasma infection and tick habitat suitability. the aims of this study were (i) to characterize by 16s rrna and species-specific msp4 gene pcr the prevalence and genotypes of a. marginale, a. phagocytophilum, and a. ovis in the most abundant host species in sicilian provinces and ...200818978093
increased prevalence of rickettsia aeschlimannii in castilla y león, spain.we report the increased prevalence recorded in recent years of rickettsia aeschlimannii in hyalomma marginatum marginatum ticks removed from human subjects in castilla y león (nw, spain). additionally, a simultaneous infection with r. aeschlimannii and anaplasma phagocytophilum in two h. marginatum marginatum ticks is reported for the first time.200918998174
first direct detection of rickettsial pathogens and a new rickettsia, 'candidatus rickettsia barbariae', in ticks from sardinia, italy.the present study evaluated the molecular detection and identification of rickettsia species in 83 ticks collected in sardinia, italy. fifteen ticks were pcr-positive using glta-specific and ompa-specific primers, leading to the identification of rickettsia aeschlimannii in hyalomma marginatum marginatum, r. massiliae in rhipicephalus turanicus and in rhipicephalus sanguineus, and a new rickettsia, previously referred to as potirb169 in portugal, in four rhipicephalus turanicus. this new species ...200819040474
towards an understanding of the migration of crimean-congo hemorrhagic fever virus.crimean-congo haemorrhagic fever (cchf) is a lethal disease caused by crimean-congo hemorrhagic fever virus (cchfv). it is one of the most widespread medically significant tick-borne pathogens, with a distribution that coincides well with the geographical occurrence of its tick vector, hyalomma marginatum marginatum. sporadic outbreaks of cchf have previously been recognized in asia, africa, the middle east and europe but, in the 21st century, outbreaks have become more frequent in former yugosl ...201019812264
subolesin expression in response to pathogen infection in ticks.ticks (acari: ixodidae) are vectors of pathogens worldwide that cause diseases in humans and animals. ticks and pathogens have co-evolved molecular mechanisms that contribute to their mutual development and survival. subolesin was discovered as a tick protective antigen and was subsequently shown to be similar in structure and function to akirins, an evolutionarily conserved group of proteins in insects and vertebrates that controls nf-kb-dependent and independent expression of innate immune res ...201020170494
bm86 homologues and novel ataq proteins with multiple epidermal growth factor (egf)-like domains from hard and soft ticks.tick control on livestock relies principally on the use of acaricides but the development of acaricide resistance and concerns for environmental pollution underscore the need for alternative control methods, for instance through the use of anti-tick vaccines. two commercial vaccines based on the recombinant bm86 protein from rhipicephalus (boophilus) microplus ticks were developed. partial protection of the bm86 vaccine against other rhipicephalus (boophilus) and hyalomma tick species suggests t ...201020647015
tick-borne rickettsioses, neglected emerging diseases in rural senegal.rickettsioses are one of the most important causes of systemic febrile illness among travelers from developed countries, but little is known about their incidence in indigenous populations, especially in west africa.201020856858
effect of various levels of dietary jatropha curcas seed meal on rabbits infested by the adult ticks of hyalomma marginatum marginatum i. animal performance, anti-tick feeding and haemogram.the goal of this study was to investigate the use of jatropha curcas seed meal (jcsm) in different levels as acaricide in diet of rabbits experimentally infested by hyalomma marginatum marginatum then determining animal performance, anti-tick feeding and its effects on haemogram of rabbits. thirty healthy mixed-breed rabbits were randomly divided into five equal groups. the first group was kept as a control fed soya bean meal (20%) as a source of protein. groups from the second to the fifth fed ...201020882342
pathogens and symbionts in ticks: a survey on tick species distribution and presence of tick-transmitted micro-organisms in sardinia, italy.a total of 1485 adult ticks were collected from mammalian hosts in south-eastern sardinia, italy, during the years 2007-2008. ticks were identified and tested by pcr analysis for presence of rickettsia species of the spotted fever group, ehrlichia canis, anaplasma phagocytophilum, coxiella burnetii, bartonella species and leishmania species. among all tick species examined (rhipicephalus sanguineus, rhipicephalus turanicus, rhipicephalus bursa, rhipicephalus pusillus, hyalomma marginatum margina ...201020884769
ixodid ticks in bosnia and herzegovina.ticks of the ixodidae family represent an enormous threat to human and animal health. from january to december 2004, a total of 10,050 ixodid ticks were collected from 26 areas in bosnia and herzegovina and determined to the species level. ticks were collected from dogs, sheep, cows, goats, rodents, humans and plants. ixodes ricinus was the most prevalent species, followed by dermacentor marginatus marginatus, rhipicephalus bursa, hyalomma marginatum marginatum, rhipicephalus sanguineus, haemaph ...201020967487
prevalence of ixodid ticks on cattle and sheep southeast of iran.a survey was carried out to investigate the prevalence of hard tick species (acari: ixodidae) on cattle and sheep southeast of iran. a total of 972 ticks were collected from 280 infested cattle and 1,207 ticks were collected from 632 infested sheep during activating seasons of ticks in 2008-2009. the species collected from cattle were hyalomma marginatum marginatum (50.92%), hyalomma anatolicum excavatum (25.61%), hyalomma anatolicum anatolicum (8.12%), hyalomma asiaticum asiaticum (1.85%), and ...201021113660
spotted fever group rickettsiae in ticks in cyprus.in two surveys conducted from march 1999 to march 2001 and from january 2004 to december 2006, a total of 3,950 ticks (belonging to ten different species) were collected from seven domestic and wild animals (goat, sheep, cattle, dog, fox, hare, and mouflon) from different localities throughout cyprus. in order to establish their infection rate with spotted fever rickettsiae (sfg), ticks were pooled and tested by polymerase chain reaction targeting glta and ompa genes, followed by sequencing anal ...201121833539
detection of new francisella-like tick endosymbionts in hyalomma spp. and rhipicephalus spp. (acari: ixodidae) from bulgaria.we report on the identification of two new francisella-like endosymbionts (fles) found in three different tick species from bulgaria. the fles were characterized by 16s rrna and tul4 gene sequencing and seem to lack the molecular marker rd1. these two new taxa seem to be facultative secondary endosymbionts of ticks.201121705542
Species distribution and detection of Crimean Congo Hemorrhagic Fever Virus (CCHFV) in field-collected ticks in Ankara Province, Central Anatolia, Turkey.Ticks may act as vectors for a number of infectious diseases including Crimean Congo Hemorrhagic Fever (CCHF). The causative agent is Crimean Congo Hemorrhagic Fever Virus (CCHFV), a member of Bunyaviridae, causing extensive ecchymosis, visceral bleeding and hepatic dysfunction with a high fatality rate in the affected individuals. CCHF was initially recognized in Turkey in 2002 and the current number of reported cases exceeds 4,400. This study was conducted to confirm the presence of tick speci ...201221910017
detection of microbial agents in ticks collected from migratory birds in central italy.tick species characterization and molecular studies were performed within ornithological surveys conducted during 2010 and 2011 in the lazio region of central italy. a total of 137 ticks were collected from 41 migratory birds belonging to 17 species (four partial migrants and 13 long-distance migrants). most ticks were nymphs, with a predominance of hyalomma marginatum marginatum and h. m. rufipes, and a small portion of ixodes and amblyomma species. all tick species analyzed were infected, and ...201424576218
identification, distribution and population dynamics of francisella-like endosymbiont in haemaphysalis doenitzi (acari: ixodidae).francisella-like endosymbionts (fles) with significant homology to francisella tularensis (γ-proteobacteria) have been characterized in several tick species, whereas knowledge on their distribution and population dynamics in ticks remains meager. hence, in the current study, we identified a novel francisella-like endosymbiont (fles-hd) from the tick haemaphysalis doenitzi and evaluated the putative functions of this symbiont. results indicated that fles-hd had 100% infection rate and a perfect v ...201627731377
the complete sequence of a west nile virus lineage 2 strain detected in a hyalomma marginatum marginatum tick collected from a song thrush (turdus philomelos) in eastern romania in 2013 revealed closest genetic relationship to strain volgograd 2007.in this study the first complete sequence of the west nile virus (wnv) lineage 2 strain currently circulating in romania was determined. the virus was detected in a hyalomma marginatum marginatum tick collected from a juvenile song thrush (turdus philomelos) in the romanian danube delta close to the city of tulcea, end of august 2013. our finding emphasizes the role of ticks in introduction and maintenance of wnv infections. sequence analyses revealed close genetic relationship of the romanian w ...201425279973
update on tick-borne rickettsioses around the world: a geographic approach.tick-borne rickettsioses are caused by obligate intracellular bacteria belonging to the spotted fever group of the genus rickettsia. these zoonoses are among the oldest known vector-borne diseases. however, in the past 25 years, the scope and importance of the recognized tick-associated rickettsial pathogens have increased dramatically, making this complex of diseases an ideal paradigm for the understanding of emerging and reemerging infections. several species of tick-borne rickettsiae that wer ...024092850
multiple pathogens including potential new species in tick vectors in côte d'ivoire.our study aimed to assess the presence of different pathogens in ticks collected in two regions in côte d'ivoire.201626771308
mediterranean spotted fever in southeastern romania.although cases of mediterranean spotted fever (msf) have been reported for decades in southeastern romania, there are few published data. we retrospectively studied 339 patients, diagnosed with msf at the national institute of infectious diseases "prof. dr. matei bals" between 2000 and 2011, in order to raise awareness about msf in certain regions of romania. according to the raoult diagnostic criteria 171 (50.4%) had a score >25 points. mean age was 52.5 years. one hundred and fifty-five (90.6% ...201324024190
characterisation of tropomyosin and paramyosin as vaccine candidate molecules for the poultry red mite, dermanyssus gallinae.dermanyssus gallinae is the most economically important haematophagous ectoparasite in commercial egg laying flocks worldwide. it infests the hens during the night where it causes irritation leading to restlessness, pecking and in extreme cases anaemia and increased cannibalism. due to an increase in the occurrence of acaricide-resistant d. gallinae populations, new control strategies are required and vaccination may offer a sustainable alternative to acaricides. in this study, recombinant forms ...201627733192
microscopic and molecular detection of theileria (babesia) equi infection in equids of kurdistan province, iran.equine piroplasmosis (ep) is the cause of persistent tick-borne infection with no symptoms, but the most important problem of ep is due to the persistent carrier state. carrier animals to babesia (theileria) equi (laveran 1901) and b. caballi (nuttall, 1910) infestation could be identified by extremely sensitive pcr-based method. the purpose of this study was to identify the causative agents of equine piroplasmosis based on molecular and microscopic assays in equids from kurdistan province, iran ...027095973
evaluation of prognostic values of tissue plasminogen activator and plasminogen activator inhibitor-1 in crimean-congo hemorrhagic fever patients.crimean-congo hemorrhagic fever (cchf) is a widespread disease in turkey, and was responsible for many deaths in endemic regions during the last decade. the pathogenesis of the disease is not fully understood yet.201526587219
challenges posed by tick-borne rickettsiae: eco-epidemiology and public health implications.rickettsiae are obligately intracellular bacteria that are transmitted to vertebrates by a variety of arthropod vectors, primarily by fleas and ticks. once transmitted or experimentally inoculated into susceptible mammals, some rickettsiae may cause febrile illness of different morbidity and mortality, and which can manifest with different types of exhanthems in humans. however, most rickettsiae circulate in diverse sylvatic or peridomestic reservoirs without having obvious impacts on their vert ...201525954738
draft genome sequence of rickettsia aeschlimannii, associated with hyalomma marginatum ticks.rickettsia aeschlimannii is a tick-associated human pathogen. we report here the draft genome of r. aeschlimannii strain mc16, isolated from hyalomma marginatum marginatum ticks collected in morocco.201425059861
prevalence of ixodid tick infestation of sheep in the arasbaran region of iran.this survey was carried out in sheep herds of arasbaran region in northwest of iran. the aims of this survey were assessment of occurrence hard ticks on sheep in this region and speciation and identification of detached ticks from sheep. in addition determination of tick distribution on sheep body surface was studied. in this study 330 sheep were examined and ticks from infested animals were collected. in parasitological laboratory speciation of ticks were done by stereomicroscope and using of t ...201224082534
colorimetric nucleic acid testing assay for rna virus detection based on circle-to-circle amplification of padlock probes.we developed a molecular diagnostic method for detection of rna virus based on padlock probes and colorimetric readout. the feasibility of our approach was demonstrated by using detection of crimean-congo hemorrhagic fever (cchf) virus as a model. compared with conventional pcr-based methods, our approach does not require advanced equipment, involves easier assay design, and has a sensitivity of 10(3) viral copies/ml. by using a cocktail of padlock probes, synthetic templates representing differ ...201121956984
synanthropic birds associated with high prevalence of tick-borne rickettsiae and with the first detection of rickettsia aeschlimannii in hungary.the aim of this study was to analyze synanthropic birds as risk factors for introducing ticks and tick-borne pathogens into human settlements, with an emphasis on rickettsiae. altogether 184 subadult ticks were found on 5846 birds. tick infestation was most prevalent during the spring. in this sample group the majority of ticks were molecularly identified as ixodes ricinus, and three individuals collected from the european robin as hyalomma marginatum marginatum. the latter is the first molecula ...201323289394
molecular and serological detection of theileria equi and babesia caballi infection in horses and ixodid ticks in iran.equine piroplasmosis is a hemoprotozoan tick-borne disease with worldwide distribution that is caused by theileria equi and babesia caballi. however, the geographical distribution of equine piroplasmosis in iran is unknown. the aim of the current study was to determine the causative agents and vector ticks of equine piroplasmosis in horses in the north khorasan province. in the year 2011, 100 horses were randomly selected from 14 villages. blood samples and ixodid ticks were collected and examin ...201424556274
crimean-congo hemorrhagic fever virus-tick survey in endemic areas in bulgaria.the balkan peninsula and bulgaria in particular, is a well-known endemic region for crimean-congo hemorrhagic fever (cchf). this study describes the prevalence of crimean-congo hemorrhagic fever virus (cchfv) among tick populations from areas, previously recognized with emerging cases of cchf disease in humans. these include regions from the southeastern (regions of kardzhali and haskovo) and central (region of stara zagora) parts of the country. for the period 2006-2010 a total of 911 adult tic ...201222337300
molecular investigation of francisella-like endosymbiont in ticks and francisella tularensis in ixodid ticks and mosquitoes in turkey.this study was carried out to investigate the molecular prevalence of francisella-like endosymbionts (fles) and francisella tularensis in ticks (acari: ixodidae) and mosquitoes in turkey. genomic dna pools were constructed from a total of 1477 adult hard ticks of rhipicephalus (rh.) annulatus, rh. turanicus, rh. sanguineus, rh. bursa, haemaphysalis (hae.) parva, hae. sulcata, hyalomma marginatum marginatum, h. anatolicum anatolicum, h. anatolicum excavatum, h. detritum detritum, h. dromedarii, d ...201626741324
detection of francisella-like endosymbiont in hyalomma rufipes from ethiopia.the expanding family of francisellaceae includes the genus francisella, where several pathogen bacteria, e.g. the zoonotic f. tularensis, and different francisella-like agents belong to. francisella-like endosymbionts (fles) are widespread in hard and soft ticks and their pathogenicity is unknown. the examination of 296 ticks collected in ethiopia was performed for the detection of f. tularensis and fles using polymerase chain reaction (pcr) assays based on the amplification of 16s rrna, sdha an ...201425108781
detection of rickettsia hoogstraalii, rickettsia helvetica, rickettsia massiliae, rickettsia slovaca and rickettsia aeschlimannii in ticks from sardinia, italy.tick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. the presence of rickettsia spp. was evaluated by standard pcr and sequencing in 123 ticks collected from several mammals and vegetation in sardinia, italy. this study provides the first evidence of the presence of rickettsia hoogstralii in haemaphysalis punctata and haemaphysalis sulcata ticks from mouflon and rickettsia helvetica in ixodes festai ticks from hedgehog. in addition, ric ...201728110915
rickettsia conorii israelensis in rhipicephalus sanguineus ticks, sardinia, italy.the presence of tick-borne rickettsia spp. was examined by pcr using dna samples extracted from 254 ticks collected from mammals originating from northern and eastern sardinia, italy. the spotted fever group rickettsial agent rickettsia conorii israelensis was detected in 3 rhipicephalus sanguineus ticks from a dog for the first time in this geographical area. in addition, ri. massiliae, ri. slovaca, and ri. aeschlimannii were detected in rh. turanicus, rh. sanguineus, dermacentor marginatus, an ...201424852264
molecular investigation and phylogeny of anaplasmataceae species infecting domestic animals and ticks in corsica, france.corsica is a french island situated in the mediterranean sea. the island provides suitable natural conditions to study disease ecology, especially tick-borne diseases and emerging diseases in animals and ticks. the family anaplasmataceae is a member of the order rickettsiales; it includes the genera anaplasma, ehrlichia, neorickettsia and wolbachia. anaplasmosis and ehrlichiosis traditionally refer to diseases caused by obligate intracellular bacteria of the genera anaplasma and ehrlichia. the a ...201728645313
molecular characterization of bm86 gene orthologs from hyalomma excavatum, hyalomma dromedarii and hyalomma marginatum marginatum and comparison with a vaccine candidate from hyalomma scupense.the ixodid ticks from the hyalomma genus are important pests of livestock, having major medical and veterinary significance in northern africa. beside their direct pathogenic effects, these species are vectors of important diseases of livestock and in some instances of zoonoses. anti-tick vaccines developed in australia and cuba based on the concealed antigen bm86 have variable efficacy against h. anatolicum and h. dromedarii. this variation in vaccine efficacy could be explained by the variabil ...201222683299
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