| [direct and indirect somatic embryogenesis in freesia refracta]. | somatic embryogenesis can be induced in tissue cultures of freesia refracta either directly from the epidermal cells of explant, or indirectly via intervening callus. in direct pathway, somatic embryos were in contact with maternal tissue in a suspensor-like structure. in indirect pathway, the explants first proliferacted to give rise to calluses before embryoids were induced. the two sorts of calluses were defined to embryogenic callus and non-embryogenic callus according to producing of somati ... | 1999 | 12548784 |
| successful expression in pollen of various plant species of in vitro synthesized mrna introduced by particle bombardment. | gold particles coated with beta-glucuronidase (gus) mrna with a 5' cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (lilium longiflorum), freesia (freesia refracta) and tulip (tulipa gesneriana). a fluorometric assay for the gus activity indicated that in vitro synthesized gus mrna introduced into these pollen cells by particle bombardment was successfully expressed. gus activity in extracts of the bombarded lily pol ... | 1995 | 7599318 |