| o serotyping of providencia stuartii isolates collected from twelve hospitals. | a collection of 829 isolates of providencia stuartii, mostly from urological specimens of patients in 12 hospitals, were o serotyped. hospitals varied in serotype distribution, but most isolates (97%) fell into one or another of 14 o types of p. stuartii. one type (o63) was found in 10 hospitals, and six types (o4, o17, o25, o52, o55, o56) were found in 5 or more hospitals. these seven types were more common than others and included 753 (91%) of the isolates. only four isolates agglutinated in p ... | 1979 | 429535 |
| [etiologic role of bacteria of the genus providencia in acute intestinal diseases]. | a study was made of morphological, cultural, biochemical properties and antigenic structure (by o-antigen) of 59 strains isolated in group acute intestinal affection of children at the pioneer camp. by the combination of biochemical properties (31 tests) all the cultures isolated were referred to providencia alcalifaciens of biotype 7. serological typing with the aid of experimental diagnostic agglutinating o-sera showed these strains to be referred to serological group o2. identity of the o-ant ... | 1977 | 602540 |
| pathogenesis of providencia alcalifaciens-induced diarrhea. | providencia alcalifaciens is a member of the family enterobacteriaceae. there are reports that p. alcalifaciens can cause diarrhea, but the mechanism(s) by which it causes diarrhea is known. we studied p. alcalifaciens isolated from a child and two adults with diarrhea for enteropathogenicity. the three isolates did not exhibit any characteristic adherence to cultured hep-2 cell monolayers, and they did not produce enterotoxins, cytotoxins, or keratoconjunctivitis in the sereny test. two isolate ... | 1992 | 1452332 |
| studies of bacterial, rotaviral and cryptosporidium etiology of acute diarrheal diseases in hospitalized children. | 657 hospitalized children with acute diarrheal disease were studied for bacterial and rotaviral etiology. cryptosporidium presence was followed in 123 children. intestinal pathogens were detected in 195 (29.6%) cases: 132 (20.3%) enterobacteria, 47 (7.1%) rotaviruses, 4 (3.2%) cryptosporidium sp. and 12 (1.8%) combined infections. among enterobacteria, e. coli was the most frequent (10% cases) with enteropathogenic (epec), enterotoxigenic (etec), enterohemorrhagic (ehec) and enteroinvasive (eiec ... | 1991 | 1666314 |
| providencia alcalifaciens and travellers' diarrhoea. | | 1989 | 2504344 |
| identification of porins in outer membrane of proteus, morganella, and providencia spp. and their role in outer membrane permeation of beta-lactams. | proteus mirabilis, proteus vulgaris, morganella morganii, providencia rettgeri, and providencia alcalifaciens, which were once classified into the same genus, proteus, were studied. cefoxitin-resistant mutants from these species were isolated, and it was confirmed that the resistance was attributed to the lack of an outer membrane protein, resulting in a significant decrease in the penetration of hydrophilic cephalosporins through the outer membrane. comparison of the mutant strains with their p ... | 1987 | 3034144 |
| [evaluation on the capability of indole pyruvic acid production by members of proteeae on different brands of sulfide-indole-motility]. | members of proteeae were vertically inoculated into different brands of sulfide-indole-motility (sim) medium for evaluating the capability of ipa production. a total of 328 clinical strains of tribe proteeae was used as tested organisms, including 186 proteus mirabilis strains, 62 morganella morganii strains, 31 proteus vulgaris strains, 27 providencia rettgeri strains, 14 providencia stuartii and 8 providencia alcalifaciens strains. seven different brands of sim medium used in this study are ky ... | 1988 | 3197461 |
| commercial identification systems often fail to identify providencia stuartii. | we tested 145 clinical isolates in an attempt to evaluate some of the most widely used commercial identification systems in europe in terms of their ability to identify providencia strains. two manual miniaturized systems (api 20e and enterotube ii) and three mechanized-automated systems (cobas-bact, sceptor system, and titertek-enterobac-rapid automated system) were evaluated. providencia alcalifaciens and providencia rettgeri strains were correctly identified by all systems in all cases, and i ... | 1988 | 3277997 |
| numerical analysis of electrophoretic protein patterns of providencia alcalifaciens strains from human faeces and veterinary specimens. | twenty-five strains of providencia alcalifaciens from various countries have been characterized by one-dimensional sds-page of cellular proteins. they comprised 15 from human faeces, one from duck faeces, one from a guinea-pig eye and eight from unknown sources. also included, for reference purposes, were the type strains of three other providencia species. the protein patterns, which contained 45-50 discrete bands, were highly reproducible and were used as the basis for two numerical analyses. ... | 1988 | 3350783 |
| [interest of gamma-glutamyltransferase in "enterobacteriaceae" (author's transl)]. | gamma-glutamyltransferase (gammagt) could be detected in 86,6% of 3,027 strains of enterobacteriaceae, by the use of gamma-l-glutamin-p-nitranilide acid for substrate. the following species produced gamma gt: citrobacter freundii, levinea malonatica, l. amalonatica, klebsiella pneumoniae, k. oxytoca, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, k. ozaenae, enterobacter aerogenes, e. cloacae, e. agglomerans, e. gergoviae, hafnia alvei, erwinia carotovora, serratia ... | 1980 | 6104464 |
| [characteristics of the fatty acid composition of providencia alcalifaciens and providencia stuartii lipids]. | the fatty acid composition of total cellular lipids and lipid a of lipopolysaccharides was studied in providencia alcalifaciens and p. stuartii. significant differences were found in the fatty acid composition of total cellular lipids from the above species; these are caused mainly by the presence of cyclopropane fatty acids (methylenehexadecanoic and methyleneoctadecanoic acids) in p. stuartii and their absence in p. alcalifaciens. differences were also established in the content of fatty acids ... | 1982 | 7070309 |
| enteropathogens associated with acute diarrhoeal diseases. | five types of escherichia coli are responsible for as much as 25% of all diarrheal diseases in developing countries. they tend to be transmitted via contaminated foods, particularly weaning foods, and water. they include enterotoxigenic, enteropathogenic, enteroadherent, enteroinvasive, and enterohemorrhagic e. coli. shigella species are responsible for 10-15% of acute diarrheas in children less than 5 years old and the most common etiologic agents of childhood dysentery. shigellosis is common i ... | 1994 | 7835992 |
| [the isolation and acylase (n-acylamino acid amidohydrolase) characteristics of providencia alcalifaciens]. | | 1994 | 7879514 |
| prevalence of invasive ability and other virulence-associated characteristics in providencia alcalifaciens strains isolated in são paulo, brazil. | providencia alcalifaciens is an invasive enteric pathogen. the present study determined the prevalence of invasive ability in p. alcalifaciens strains isolated in são paulo, brazil, mainly from patients with diarrhoea. invasion of hela cells was found in 17 (42%) of 41 strains studied. most (88%) of the invasive strains were isolated from diarrhoeal stools. the invasive property was identified in 50% of p. alcalifaciens strains isolated as pure cultures or from stool samples where no other enter ... | 1996 | 8958250 |
| invasion of hela cells by providencia alcalifaciens presumably is plasmid-encoded. | | 1996 | 9283662 |
| electron microscopic study of the attachment and penetration of rabbit intestinal epithelium by providencia alcalifaciens. | ilea of adult rabbits with removable ileal ties (ritard model) that developed diarrhoea and ileal loops of adult rabbits after inoculation with providencia alcalifaciens, isolated from two patients with diarrhoea, were studied by transmission electron microscopy for enterocyte-bacterial interactions. two modes of entry of bacteria into epithelial cells were seen: one directly by endocytosis associated with polymerization of cytoskeletal components and the other by disruption of tight junctions w ... | 1993 | 8229460 |
| characteristics of invasion of hep-2 cells by providencia alcalifaciens. | previous studies with three isolates from diarrhoeal stools suggested that providencia alcalifaciens is an invasive enteric pathogen that also causes actin condensation in infected cells. these findings were extended in the present study with a further 14 diarrhoeal stool isolates of p. alcalifaciens and hep-2 cell monolayers for invasion assays. studies on invasion characteristics with two selected isolates suggested that p. alcalifaciens required prior growth at 37 degrees c for better invasio ... | 1995 | 7884799 |
| spot indole test: evaluation of four reagents. | kovacs indole reagent, p-dimethylaminobenzaldehyde, ehrlich indole reagent and p-dimethylaminocinnamaldehyde were used as spot indole reagents to test 359 strains of gram-negative rods growing on 5% sheep blood agar, trypticase soy agar (bbl microbiology systems), and macconkey agar. the p-dimethylaminocinnamaldehyde reagent was the most sensitive of those tested and provided results that were easiest to interpret. the p-dimethylaminocinnamaldehyde reagent was able to detect providencia alcalifa ... | 1982 | 7040458 |
| genetic recombination between proteus mirabilis and providencia alcalifaciens. | chromosome transfer occurred in plate matings between proteus mirabilis strain pm5006 and providencia alcalifaciens strain p29 in either direction with the use of plasmid d or r772 as sex factor. auxotrophic chromosomal markers of recipients were converted to prototrophy and the galactose fermentation marker of donor pm5006 could also be selected. recombination frequencies for a group of selected markers in pm5006(d) x p29 matings varied between 3 x 10(-5) (trp+) and 1.2 x 10(-7) (lys+) per dono ... | 1980 | 7012276 |
| providencia rustigianii: a new species in the family enterobacteriaceae formerly known as providencia alcalifaciens biogroup 3. | the name providencia rustigianii sp. nov. is proposed for a group of organisms previously known as providencia alcalifaciens biogroup 3. by dna hybridization, strains of p. rustigianii were 81 to 99% related to each other at 60 degrees c, but only 44 to 49% related to p. alcalifaciens biogroups 1 and 2 and 26 to 33% related to providencia stuartii. p. rustigianii could be differentiated from p. alcalifaciens and p. stuartii by simple biochemical tests. p. rustigianii produced acid from d-galacto ... | 1983 | 6874899 |
| comparative in vitro activity of semisynthetic penicillins against proteeae. | the in vitro susceptibilities of 181 isolates of precisely identified proteeae species to five semisynthetic penicillins were determined with low and high inocula. significant differences in susceptibility patterns among various proteeae species to the penicillins examined were demonstrated. providencia stuartii was clearly distinguished from providencia alcalifaciens by its greater resistance to the antibiotics tested. | 1983 | 6859841 |
| species differences in susceptibilities of proteeae spp. to six cephalosporins and three aminoglycosides. | six cephalosporins and three aminoglycosides were examined for activity against 1,693 isolates belonging to six species of proteeae. the most notable species-specific differences included the marked susceptibility of providencia alcalifaciens and proteus mirabilis to cephalothin, the resistance of proteus vulgaris to cefamandole, and the resistance of providencia stuartii to gentamicin and tobramycin. the third-generation cephalosporins cefotaxime and moxalactam were substantially more inhibitor ... | 1982 | 6765415 |
| freeze-dried mixed cultures as samples for proficiency tests and collaborative studies in food microbiology. | a method is presented for preparing samples of freeze-dried mixtures of microorganisms for proficiency tests and collaborative studies. the samples may include most microorganisms that are found in routine analysis in food laboratories. transport of samples during 48 h did not decrease the number of microorganisms, nor was the variability among samples significantly affected by transport. the standard deviation of counts after 5 weeks of storage varied from 0.04 (staphylococcus aureus) to 0.17 ( ... | 1983 | 6643366 |
| mechanical carrier of bacterial enteric pathogens by chrysomya megacephala (diptera: calliphoridae) in chiang mai, thailand. | chrysomya megacephala was studied regarding its mechanically bacterial carrier in urban areas of chiang mai, northern thailand. fifty-six adult flies were randomly collected using sweep insect net during april-may, 1999 from 3 fresh food markets and examined for bacteriological isolation. among them, 49 flies (87.5%) were bacterial carriers. the total 22 bacterial species and 8 groups were isolated. three species previously reported as the bacterial enteric pathogens causing diarrheal disease we ... | 2000 | 11414447 |
| panophthalmitis and otitis interna in fire-bellied toads. | microbiologic and histologic studies were made of fire-bellied toads with signs of ocular and central nervous system disease. providencia alcalifaciens, citrobacter freundii, aeromonas hydrophila, and other gram-negative bacilli were isolated from the eyes and multiple tissues of ill toads. the histologic evaluations revealed severe panophthalmitis and otitis interna. | 1983 | 6643232 |
| non-invasive providencia alcalifaciens strains fail to attach to hep-2 cells. | we investigated the adherence properties of six p. alcalifaciens strains with previously characterized differential invasive capabilities in hep-2 cells. highly invasive strains were found to attach to hep-2 cell monolayers within 2 h post-infection and in large numbers on the eukaryotic cell surfaces within 3 h post-infection. in contrast, weakly or non-invasive p. alcalifaciens strains were non-adherent to hep-2 cells even at 3 h post-infection. highly invasive isolates were found to weakly bi ... | 2001 | 11685508 |
| phage x: a plasmid-dependent, broad host range, filamentous bacterial virus. | phage x was isolated from sewage as plating on escherichia coli or salmonella typhimurium strains harbouring the incompatibility group x plasmid r6k. it also plated on a strain of serratia marcescens carrying this plasmid. it failed to form plaques on proteus mirabilis, p. morganii or providencia alcalifaciens harbouring r6k, but did multiply on them. no phage increase occurred with homologous r- strains. phage x also plated or registered an increase in titre on e. coli or s. typhimurium strains ... | 1981 | 6121839 |
| fatty acid composition of paracolons: arizona, citrobacter, and providencia. | the fatty acid compositions of stationary-phase cultures of arizona arizonae, citrobacter freundii, providencia alcalifaciens, providencia stuartii, and providencia sp. were studied. the major fatty acids of a. arizonae, c. freundii, and providencia were 16:0, 16:1, 17:cyclopropane, and 19:cyclopropane. the fatty acid compositions of the two strains of a. arizonae examined were similar to each other, but the three strains of c. freundii differed from one another in their fatty acid compositions. ... | 1973 | 4698216 |
| providencia alcalifaciens in diarrhoeic dogs and cats. | | 2002 | 11936417 |
| [enteropathogenicity of providencia alcalifaciens, a newly recognized pathogen causing food-poisoning]. | providencia alcalifaciens, a member of the family enterobacteriaceae, has been thought to be a normal flora or an opportunistic pathogen. in 1996, a large outbreak (including 270 patients) of acute gastroenteritis occurred in fukui. no known enteropathogens were detected in fecal samples of the patients, but p. alcalifaciens was detected from 7 of the 18 samples. the isolates were a clonal by pulsed-field gel electrophoresis. the specific antibody against the isolated strain was elevated in the ... | 2002 | 12078099 |
| [electrophoretic polymorphism of lactate, malate and glutamate dehydrogenases, acid phosphatase and esterases of providencia alcalifaciens, p. stuartii and p. rustigianii]. | the polymorphism of glutamate, lactate and malate dehydrogenases, of acid phosphatase and of esterases of 27 strains of providencia alcalifaciens, 35 strains of p. stuartii and 17 strains of p. rustigianii was investigated by conventional electrophoresis in polyacrylamide agarose gel and by isoelectric focusing in thin-layer polyacrylamide gel. for each enzyme analysed, the three species were characterized by a distinct electrophoretic pattern. the number of allozymes detected by conventional el ... | 1985 | 4051454 |
| [isolation and properties of aminocaprolactam hydrolase from providencia alcalifaciens]. | l-alpha-aminocaprolactam hydrolase possessing the l-lysinamidase activity was isolated and purified from providencia alcalifaciens. the purification procedure of enzymes included cell destruction on usdl-1, fractionation by ammonium sulfate, gel-chromatography on g-100, ion exchange chromatography on deae-cellulose. the purification resulted in a homogeneous enzyme which possessed the both activities. the enzyme molecular weight (180 kda) was estimated by gel chromatography on sephadex g-200. km ... | 1987 | 3810885 |
| an evaluation of allozyme amino acid substitutions for the study of molecular relationships in providencia strains. | through analysis of molecular relationships in terms of amino acid substitutions, intra- and interspecies differentiations in providencia alcalifaciens, p. stuartii and p. rustigianii were evaluated among the electrophoretic variants of three enzymes, l-malate dehydrogenase, acid phosphatase and esterase-beta a, chosen for their distinct pattern of polymorphism. for each enzyme, molecular relatedness among variants defined by two-dimensional electrophoretic profiles was examined through protein ... | 1988 | 3252906 |
| rotavirus type a and other enteric pathogens in stool samples from children with acute diarrhea on the colombian northern coast. | the present study, conducted from march 1998 to july 2000, determined the etiology of acute diarrhea in 253 young children and infants from cartagena and sincelejo, colombia. in 253 stool samples, the following enteric pathogens were recovered: rotavirus type a (36.6%) as the major agent, salmonella spp (9.0%), shigella spp (8.0%), enteric pathogenic escherichia coli (6.0%), enteric hemorragic esc. coli (2.8%), providencia alcalifaciens (2.8%), aeromonas hydrophila (2.0%), yersinia enterocolitic ... | 2003 | 12687410 |
| bacteriophage d: an incd group plasmid-specific phage. | the existence of the plasmid incompatibility group d was reaffirmed as a result of compatibility experiments done on plasmids r687, r711b, r778b and r840 which were previously tentatively accepted as constituting the group. the group was further delineated by the isolation of a phage, phage d, which adsorbed specifically to incd plasmid-encoded pili produced by escherichia coli k12 strains and strains of salmonella typhimurium, proteus morganii and klebsiella oxytoca harbouring one of these plas ... | 1985 | 2870129 |
| usefulness of trehalose fermentation and l-glutamic acid decarboxylation for identification of biochemically aberrant providencia stuartii strains. | a total of 849 providencia isolates were collected during a 4-year period when an increased incidence of nosocomial providencia stuartii infection was noted in urologic wards. of these isolates, 630 were identified as p. stuartii, 206 were identified as providencia rettgeri, and 1 was identified as providencia alcalifaciens. twelve inositol-positive isolates from 10 patients (10 strains) resembled p. stuartii in fermenting trehalose but resembled p. rettgeri in fermenting d-arabitol or meso-eryt ... | 1989 | 2570791 |
| correlation between dna polymorphism and enzyme polymorphism argues in favour of the delineation of two species within providencia alcalifaciens. | ribosomal dna (rdna) polymorphism was compared to enzyme polymorphism and dna/dna hybridization data for the intraspecies differentiation of providencia alcalifaciens. dna from 27 strains previously classified into two zymotypes a1 and a2 and discriminated by two levels of dna/dna hybridization (delta tm values of 0 to 1 degree c and 6 to 10 degrees c, respectively) were analysed by southern blotting for rdna polymorphism. the ribotypes fell into two ribogroups a1 and a2, which correlated with t ... | 1991 | 1805310 |
| transformation of n epsilon-cbz-l-lysine to cbz-l-oxylysine using l-amino acid oxidase from providencia alcalifaciens and l-2-hydroxy-isocaproate dehydrogenase from lactobacillus confusus. | biotransformations were developed to oxidize n epsilon-carbobenzoxy(cbz)-l-lysine and to reduce the product keto acid to l-cbz-oxylysine. lysyl oxidase (l-lysine: o2 oxidoreductase, ec 1.4.3.14) from trichoderma viride was relatively specific for l-lysine and had very low activity with n epsilon-substituted derivatives. l-amino acid oxidase (l-amino acid: o2 oxidoreductase [deaminating], ec 1.4.3.2) from crotalus adamanteus venom had low activity with l-lysine but high activity with n epsilon-fo ... | 1992 | 1368913 |
| use of bile-esculin agar for rapid differentiation of enterobacteriaceae. | bile-esculin agar has been used for several years for the presumptive identification of group d streptococci. all members of the enterobacteriaceae family will also grow on this medium, but only certain ones can hydrolyze esculin to 6,7-dihydroxycoumarin, which reacts with iron to produce a characteristic blackening of the medium. one thousand and six cultures from clinical specimens representing 20 genera were isolated and identified. heavy inocula from fresh pure culture isolates on heart infu ... | 1975 | 1176613 |
| validation of a pcr for diagnosis of typhoid fever and salmonellosis by amplification of the hila gene in clinical samples from colombian patients. | validation of a pcr test to detect hila gene sequences of salmonella spp. was performed in blood and faeces samples from typhoid fever and salmonellosis patients. sensitivity (s), specificity (sp), positive predictive value (ppv) and negative predictive value (npv) of the pcr in blood samples were performed by testing: 37 patients with clinical diagnosis of typhoid fever, 34 of them confirmed by isolation of s. typhi from blood cultures; 35 patients infected with other pathogens corroborated by ... | 2004 | 15314194 |
| a rapid glutamic decarboxylase test for identification of bacteria. | a simple rapid glutamic decarboxylase test is described. this test was found useful in the identification of escherichia coli, shigella sp., providencia alcalifaciens, clostridium perfringens and bacteroides fragilis. | 1976 | 793504 |
| visualization of protoplast fusion and quantitation of recombination in fused protoplasts of auxotrophic strains of escherichia coli. | protoplast fusion has been used to combine genes from different organisms to create strains with desired properties. a recently developed variant on this approach, genome shuffling, involves generation of a genetically heterogeneous population of a single organism, followed by recursive protoplast fusion to allow recombination of mutations within the fused protoplasts. these are powerful techniques for engineering of microbial strains for desirable industrial properties. however, there is a prev ... | 2005 | 15974564 |
| o-serotyping providencia alcalifaciens. | the o-serotyping scheme for providencia was tested on providencia alcalifaciens isolates collected mostly from two hospitals. the specificites of the somatic (o) antigens of p. alcalifaciens were found to be different from those of providencia stuartii, and separation of the providencia typing scheme to allow separate typing of each species led to more efficient typing. all but 4 of 86 isolates were typable. eighteen serotypes occurred among 53 typable isolates obtained from a pediatric hospital ... | 1979 | 521478 |
| invasion of hep-2 and other eukaryotic cell lines by providenciae: further evidence supporting the role of providencia alcalifaciens in bacterial gastroenteritis. | wild-type strains of providencia species were evaluated for their ability to invade hep-2 monolayers based upon microscopic and semi-quantitative assays. of 14 p. alcalifaciens strains tested, 3 (17%) were found to be highly invasive, 4 (22%) moderately invasive, and the remaining 61% weakly or noninvasive. hep-2 invasion results were confirmed by thin-section electron microscopy. invasive capabilities of p. alcalifaciens were greater at higher mois (100 to 1000) than at lower inocula (<10 moi). ... | 1998 | 9688814 |
| association of providencia alcalifaciens with diarrhea in children. | it has been demonstrated in previous studies that providencia alcalifaciens can produce diarrhea by an invasive mechanism. in the present study, p. alcalifaciens was isolated from the stool specimens of 17 of 814 diarrheal children younger than 5 years of age (2.1%) and from those of 4 of 814 matched controls (0.49%) (p = 0.004), indicating that the organism is significantly associated with diarrhea. however, 71% of p. alcalifaciens-positive diarrheal children had simultaneous infections with ot ... | 1998 | 9574723 |
| [study of real-time pcr assays for rapid detection of food-borne pathogens]. | a duplex real-time sybr green lightcycler pcr (lc-pcr) assay with dna extraction using qiaamp dna stool minikit was evaluated for detection of 8 of 19 species of food-borne pathogens, including plesiomonas shigelloides, providencia alcalifaciens, in five stool specimens. the time frame was within 2h or less. the protocol used the same lc-pcr with 22 pairs of specific primers. the rapid amplification and reliable detection of specific genes were determined by this lc-pcr assay from 10 cases of fo ... | 2005 | 16248373 |
| media for the detection and recognition of the enteropathogen providencia alcalifaciens in faeces. | a medium (pam: providencia alcalifaciens medium) is described that enables the presence of the enteropathogen p. alcalifaciens in faeces to be detected with ease and simplicity. this organism is probably the only oxidase-negative organism likely to be present in tetrathionate broth cultures of faeces that is unable to ferment the mannitol, xylose or galactose present in the medium. thus the red colonies of p. alcalifaciens appeared quite distinct from the lemon-yellow acid-forming colonies of al ... | 1997 | 9350207 |
| structure of the o-specific polysaccharide of the bacterium providencia alcalifaciens o23 containing a novel component: an amide of d-glucuronic acid with n(epsilon)-(1-carboxyethyl)lysine. | an acidic o-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium providencia alcalifaciens o23 and found to contain d-glucose, d-galactose, 2-acetamido-2-deoxy-d-galactose, and n epsilon-(1-carboxyethyl)-n alpha-(d-glucuronoyl)lysine. on the basis of full and partial acid hydrolyses, selective solvolysis with anhydrous hydrogen fluoride, and 1h- and 13c-nmr spectroscopy, including two-dimensional correlation spectroscopy (cosy), h-detected hete ... | 1997 | 9275290 |
| identification of n epsilon-[(r)-1-carboxyethyl]-l-lysine in, and the complete structure of, the repeating unit of the o-specific polysaccharide of providencia alcalifaciens o23. | n epsilon-[(r)-1-carboxyethyl]-l-lysine was released by acid hydrolysis from the o-specific polysaccharide of providencia alcalifaciens o23 and identified by 1h and 13c nmr spectroscopy, glc-ms after conversion to a di-n-acetylated dimethyl ester, and by comparison with the authentic sample. solvolysis of the polysaccharide with anhydrous hf resulted in an amide of d-glucuronic acid with n epsilon-[(r)-1-carboxyethyl]-l-lysine. these and published data allowed the determination of the full struc ... | 1998 | 9720244 |
| clonal structure of providencia alcalifaciens strains isolated from diarrhoeal stools in são paulo, brazil. | clonal analysis based on ribotyping demonstrated that providencia alcalifaciens strains isolated mainly from diarrhoeal stools in são paulo, brazil, were clustered into two main groups. eleven distinct ribotype patterns were identified with clai, ecorv and mlui restriction endonucleases. p. alcalifaciens strains with invasive properties were of two ribotype patterns that differed from those identified among non-invasive strains. the ribotyping results confirmed that p. alcalifaciens strains asso ... | 1999 | 9989649 |
| structure of a neutral o-specific polysaccharide of the bacterium providencia alcalifaciens o5. | a neutral polysaccharide containing d-galactose, 2-acetamido-2-deoxy-d-glucose, and 3-acetamido-3,6-dideoxy-d-glucose (qui3nac) in the ratios 2:1:1 was obtained by mild acid degradation of lipopolysaccharide of the bacterium providencia alcalifaciens o5 followed by gel chromatography and ion-exchange chromatography or treatment with anhydrous hydrogen fluoride. on the basis of full acid hydrolysis, methylation, and 1h- and 13c-nmr spectroscopy, including two-dimensional correlation spectroscopy ... | 1999 | 10381612 |
| molecular characterization of the genera proteus, morganella, and providencia by ribotyping. | the so-called proteus-providencia group is constituted at present by three genera and 10 species. several of the recognized species are common opportunistic pathogens for humans and animals. different methods based on the study of phenotypic characters have been used in the past with variable levels of efficiency for typing some species for epidemiological purposes. we have determined the rrna gene restriction patterns (ribotypes) for the type strains of the 10 different species of the genera pr ... | 1999 | 10449462 |
| structure of an acidic o-specific polysaccharide of the bacterium providencia alcalifaciens o7. | an acidic o-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium providencia alcalifaciens o7 and purified by gel chromatography followed by anion-exchange chromatography. on the basis of full acid hydrolysis, methylation, carboxyl reduction, selective cleavage with anhydrous hydrogen fluoride, and 1h- and 13c-nmr spectroscopy, including two-dimensional 1h,1h homonuclear and h-detected 1h,13c heteronuclear correlation spectroscopy and nuclear o ... | 2000 | 10887286 |
| molecular analysis of clinical isolates of providencia alcalifaciens. | in an attempt to elucidate the virulence factors and the pathogenic mechanisms of providencia alcalifaciens, 36 isolates identified in 1994-1995 in recife city, brazil were analysed by pcr to investigate the presence of dna sequences homologous to virulence genes described in other invasive enterobacteria, as well as their ability to invade hela cells, their plasmid profiles and antibiotic resistance patterns. the genetic diversity of the isolates was also analysed by rapd-pcr. no homologous seq ... | 2001 | 11192501 |
| structure and serological characterization of an nepsilon-[(r)-1-carboxyethyl]-l-lysine-containing o-chain of the lipopolysaccharide of proteus mirabilis o13. | in this paper we present the structure and describe serological properties of the o-specific polysaccharide of proteus mirabilis o13 lipopolysaccharide, which contains a unique component: an amide of d-galacturonic acid (d-gala) with an unusual amino acid, nepsilon-[(r)-1-carboxyethyl]-l-lysine (alaninolysine, alalys). selective chemical degradations of either gala or alalys resulted in the loss of the serological reactivity of the polysaccharide with anti-o serum against p. mirabilis o13. neith ... | 2001 | 11348021 |
| structure of the o-polysaccharide of providencia alcalifaciens o8 containing (2s,4r)-2,4-dihydroxypentanoic acid, a new non-sugar component of bacterial glycans. | a glycerol teichoic acid-like o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o8 and studied by chemical methods and nmr spectroscopy, including 2d roesy, {(1)h,(13)c} hsqc, and hmqc-tocsy experiments. it was found that the compound contains a new component of bacterial lipopolysaccharides: ether-linked (2s,4r)-2,4-dihydroxypentanoic acid (dhpa), which was identified by nmr spectroscopy. the following structure of the repeating unit o ... | 2008 | 18768172 |
| a large outbreak of foodborne infection attributed to providencia alcalifaciens. | the enteropathogenicity of providencia alcalifaciens, a member of the family enterobacteriaceae, has not yet been well established. in november 1996, a large outbreak of foodborne infection occurred in fukui, japan. in this study, the etiology of the outbreak was investigated. no other recognized enteropathogens were detected in patient fecal samples, but p. alcalifaciens was detected in 7 of 18 samples. the isolates were found to be clonal by pulsed-field gel electrophoresis. the patients who p ... | 2001 | 11574921 |
| primary bacterial enteritis caused by providencia alcalifaciens in three dogs. | | 2002 | 11833525 |
| evolution of the gyrb gene and the molecular phylogeny of enterobacteriaceae: a model molecule for molecular systematic studies. | phylogenetic trees showing the evolutionary relatedness of enterobacteriaceae based upon gyrb and 16s rrna genes were compared. congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. phylogenetic trees based on gyrb sequences appeared to be more reliable at determining relationships among serratia species than trees based on 16s rrna gene sequences. gyrb sequences from serratia spec ... | 2002 | 11931166 |
| diarrhea outbreak among czech army field hospital personnel caused by providencia alcalifaciens. | | 2002 | 12088581 |
| tnphoa mutants of providencia alcalifaciens with altered invasiveness of hep-2 cells. | recent studies have shown that providencia alcalifaciens is a diarrhoeal pathogen. it may cause diarrhoea by an invasive mechanism, as it invades cultured mammalian cells in vitro and intestinal epithelial cells of experimentally inoculated rabbits in vivo. to locate the gene(s) involved in invasion, tnphoa mutants of a diarrhoeal isolate of p. alcalifaciens were generated. compared with the parent strain, these mutants exhibited negligible invasion and actin condensation in hep-2 cells. tnphoa ... | 2002 | 12171300 |
| structure of the o-specific polysaccharide of providencia alcalifaciens o16 containing n-acetylmuramic acid. | the o-specific polysaccharide of providencia alcalifaciens o16 was obtained by mild-acid degradation of the lipopolysaccharide and studied by chemical methods and nmr spectroscopy, including 2d 1h,(1)h cosy, tocsy, noesy, and 1h,(13)c hsqc experiments. it was found that the polysaccharide contains n-acetylmuramic acid, which was isolated by solvolysis with trifluoromethanesulfonic acid and identified by the specific optical rotation and nmr spectroscopy. the following structure of the trisacchar ... | 2002 | 12423969 |
| structure of the o-polysaccharide of providencia alcalifaciens o21 containing 3-formamido-3,6-dideoxy-d-galactose. | the o-polysaccharide (o-antigen) of providencia alcalifaciens o21 was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods and nmr spectroscopy. it was found that the polysaccharide is built up of branched pentasaccharide repeating units with a terminal residue of 3-formamido-3,6-dideoxy-d-galactose (d-fuc3nfo) and has the following structure: [structure: see text]. anti-p. alcalifaciens o21 serum cross-reacted with the o-antigen of proteus vulgaris o47, wh ... | 2003 | 12801716 |
| providencia alcalifaciens strains translocate from the gastrointestinal tract and are resistant to lytic activity of serum complement. | the ability of providencia alcalifaciens strains, isolated from patients with diarrhoeal disease, to translocate from the gastrointestinal tract and their resistance to serum complement lytic activity were investigated and compared with previously characterized differential invasive capabilities in hela cells. translocation ability to several extraintestinal sites and resistance to lysis by human serum complement were observed in both highly invasive and non-invasive strains. these characteristi ... | 2003 | 12867555 |
| structure of the o-polysaccharide of providencia alcalifaciens o19. | studies of the o-polysaccharide chain of the lipopolysaccharide (o-antigen) of providencia alcalifaciens o19 by sugar and methylation analyses along with nmr spectroscopy, including 2d 1h,1h cosy, tocsy, noesy and 1h,13c hsqc experiments, showed that the pentasaccharide repeating unit of the polysaccharide has the following structure: [structure: see text] where fuc3nac is 3-acetamido-3,6-dideoxygalactose. the unique structure of the o-antigen and serological data are in consistence with classif ... | 2004 | 14698901 |
| serological characterization of the o-specific polysaccharide of providencia alcalifaciens o23. | the genus providencia belongs to the enterobacteriaceae family and currently consists of five species: p. alcalifaciens, p. heimbachae, p. rettgerii, p. rustigianii and p. stuartii. the serological classification scheme of p. alcalifaciens, p. rustigianii and p. stuartii includes 63 o-serogroups and 30 h-serogroups. the o-antigenic specificity is defined by the structure of the o-antigen (o-specific polysaccharide--ops), a part of the lipopolysaccharide (lps, endotoxin), one of the major compone ... | 2004 | 15053232 |
| sanitary and bacteriological aspects of sewage treatment. | a study into the removal of contamination load and indicator bacteria was carried out in 1992-1996 in the mechanical, biological and chemical waste-water treatment plant wtp in lezany, in the county of reszel, in the province of warmia and mazury in poland. the results of chemical analyses found a high efficiency of removal of carbon compounds, cod (90%) and bod (98%), in the process of purification of household sewage. in addition, a high effectiveness of total nitrogen, on average 71%, and uns ... | 2003 | 15058814 |
| [development and evaluation of a pcr method for diagnosis of salmonella enteric fever, based on dna sequences of the hila gene]. | typically, diagnosis of enteric fever due to salmonella spp. is by bacterial isolation from blood culture; however, the blood culture method is slow, not always available, and not informative in patients with antibiotic treatment. salmonella spp. uses the hila gene (component of the pathogenicity island i) to invade epithelial cells and produce infection. using the hila gene sequence a pcr test was designed to detect salmonella in blood samples. the sensitivity (s), specificity (sp), positive pr ... | 2004 | 15495599 |
| enteric fever-like illness caused by infection with citrobacter amalonaticus. | 'enteric fever' is a potentially fatal, severe systemic disease, which is encountered worldwide. traditionally, enteric fever refers to a bacteremic illness caused by members of certain salmonella serotypes, notably: salmonella typhi, a gram-negative bacterium, and to a lesser extent, salmonella paratyphi a, b and c. in addition, other non-salmonella organisms may produce a syndrome clinically indistinguishable from "enteric fever". brucella sp., campylobacter sp., edwardsiella tarda, enterobact ... | 2005 | 16083226 |
| [providencia alcalifaciens as a newly recognized cause of food-borne disease]. | | 2005 | 16180691 |
| importance of providencia species as a major cause of travellers' diarrhoea. | in this study the importance of providencia species as a cause of travellers' diarrhoea was examined using a selective medium developed by the authors. providencia species could easily be distinguished from other enteric pathogens by the colour of the colonies obtained. nine strains of providencia alcalifaciens, nine of providencia rettgeri and five of providencia stuartii were isolated from 130 specimens, representing a surprisingly high incidence of infection compared with other pathogens isol ... | 2005 | 16192440 |
| the structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o30. | the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o30. studies by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including two-dimensional (1)h,(1)h cosy, tocsy, roesy, and h-detected (1)h,(13)c hsqc, hmbc, and hmqc-tocsy experiments, showed that the polysaccharide has a linear pentasaccharide repeating unit of the following structure: | 2006 | 16472790 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o29. | the o-polysaccharide was obtained by a mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o29. structural studies were performed using sugar and methylation analyses along with 1h and 13c nmr spectroscopy, including two-dimensional 1h, 1h cosy, tocsy, roesy, h-detected 1h, 13c hsqc and hmbc experiments. on the basis of the data obtained, the following structure of the branched tetrasaccharide repeating unit of the o-polysaccharide was established: [structure: see text]. | 2006 | 16630597 |
| the structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o36 containing 3-deoxy-d-manno-oct-2-ulosonic acid. | an oligosaccharide that corresponds to the repeating unit of the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o36. structural studies of the oligosaccharide and o-deacylated lipopolysaccharide were performed using sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy, and h-detected (1)h,(13)c hsqc and hmbc experiments. it was found that the o-polysaccharide is built up ... | 2007 | 16815342 |
| rapid separation and concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time pcr. | buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as pcr, and to prevent false-positive results due to dna originating from dead cells. applying a principle of buoyant density gradient centrifugation, we developed a method for rapid separation and concentration following filtration and low- and high-speed centrifugation, as well as flotation and sedimentation buoyant den ... | 2007 | 17056684 |
| structure of the o-polysaccharide and serological cross-reactivity of the lipopolysaccharide of providencia alcalifaciens o32 containing n-acetylisomuramic acid. | the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o32 and studied by sugar and methylation analyses, solvolysis with triflic acid, 1h and 13c nmr spectroscopy, including two-dimensional 1h,1h cosy, tocsy, roesy, h-detected 1h,13c hsqc and hmbc experiments. it was found that the polysaccharide has a branched tetrasaccharide repeating unit containing 2-acetamido-3-o-[(s)-1-carboxyethyl]-2-deoxy-d-glucose (d-glcnac3slac, n-acetylisomur ... | 2007 | 17182016 |
| new structure for the o-polysaccharide of providencia alcalifaciens o27 and revised structure for the o-polysaccharide of providencia stuartii o43. | the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide from providencia alcalifaciens o27 and studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy, h-detected (1)h,(13)c hsqc, and hmbc experiments. it was found that the polysaccharide is built up of linear partially o-acetylated tetrasaccharide repeating units and has the following structure: [structure: see text] where qui4nfo stands for 4-f ... | 2007 | 17368583 |
| structure of a colitose-containing o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o6. | the o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o6 and studied by sugar and methylation analysis, selective hydrolytic removal of 3,6-dideoxy-l-xylo-hexose (colitose, col), (1)h and (13)c nmr spectroscopy, including 2d (1)h,(1)h cosy, tocsy, roesy and h-detected (1)h,(13)c hsqc and hmbc experiments. the polysaccharide was found to have a branched pentasaccharide repeating unit with the following structure: [see text] remarkably, t ... | 2007 | 17559819 |
| demonstration and characterization of manganese superoxide dismutase of providencia alcalifaciens. | superoxide dismutases convert superoxide anions to molecular oxygen and hydrogen peroxide. these enzymes constitute one of the major defense mechanisms of cells against oxidative stress and play a role in the pathogenesis of certain invasive bacteria. in this study, we reported for the first time here that providencia alcalifaciens, a member of the family enterobacteriaceae, produces a superoxide dismutase (sod) as a major protein in culture supernatants. this protein was purified by a series of ... | 2007 | 17951985 |
| providencia alcalifaciens as the presumptive cause of diarrhoea in dog. | a case of diarrhoea in a four-month-old golden retriever is described. on the basis of anamnesis and bacteriological examination, the diagnosis of bacterial enteritis due to providencia alcalifaciens was reached. although there are contradictory opinions about the role of this organism as the enteric pathogen, it seems that providencia alcalifaciens should also be taken into consideration in the routine bacteriological diagnostics of diarrhoea in dogs. | 2007 | 18198546 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o31 containing an ether of d-mannose with (2r,4r)-2,4-dihydroxypentanoic acid. | recently, ether-linked diastereomeric 2,4-dihydroxypentanoic acids have been reported as new components of bacterial glycans [shashkov, a. s. et al.nat. prod. commun.2008, 3, 1625-1630]. in this work, an ether of (2r,4r)-2,4-dihydroxypentanoic acid (dhpa) with d-mannose was identified in the o-polysaccharide of providencia alcalifaciens o31, and the polysaccharide structure was elucidated. studies by nmr spectroscopy confirmed the ether linkage between o-2 of dhpa and o-4 of man, and the absolut ... | 2009 | 19185853 |
| [bacterial antigen polysaccharides. 43. structure of o-specific polysaccharide of providencia alcalifaciens o46]. | acid o-specific polysaccharide containing d-glucose, d-glucuronic acid, l-fucose, and 2-acetamido-2-deoxy-d-glucose was obtained by mild acid degradation of lipopolysaccharide from providencia alcalifaciens o46. consideration of the data revealed the following structure of the hexasaccharide repeating unit of o-specific polysaccharide under methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d (1)h, (1)h-cosy, tocsy-, roesy-,(1)h, (13)c-hsqc-, and hmqc-tocsy experiments: ... | 2009 | 19621056 |
| n-(1-carboxyethyl)alanine (alanopine), a new non-sugar component of lipopolysaccharides of providencia and proteus. | o-polysaccharides were released by mild acid degradation of lipopolysaccharides of providencia alcalifaciens o35 and proteus vulgaris o76 and were studied by 1d and 2d (1)h and (13)c nmr spectroscopies, including hmbc and noesy (roesy) experiments. both polysaccharides were found to contain n-(1-carboxyethyl)alanine (alanopine) that is n-linked to 4-amino-4,6-dideoxyglucose. analysis of published data [vinogradov, e.; perry, m. b. eur. j. biochem.2000, 267, 2439-2446] shows that alanopine is pre ... | 2009 | 19651402 |
| prevalence and characteristics of cytolethal distending toxin-producing escherichia coli from children with diarrhea in japan. | in the present study, we examined the prevalence and characteristics of ctec among diarrheal children in japan during a year-long surveillance study. a pcr-rflp assay for the detection and differentiation of five types of e. coli cdtb gene (types i through v) was developed, and 362 stool specimens collected from patients reporting to pediatric departments in two hospitals were analyzed. of the 35 samples (9.7%) that were positive for the cdtb gene, 21 were positive for cdt-i, three for cdt-ii, f ... | 2009 | 19714857 |
| recovery and screening for antibiotic susceptibility of potential bacterial pathogens from the oral cavity of shark species involved in attacks on humans in recife, brazil. | the number of incidents involving sharks and humans at beaches in recife, on the north-eastern brazilian coast, is among the highest worldwide. in addition, wound infections in survivors are common; but the nature and risk of the aetiological agents is unknown. in the present study, 81 potential bacterial pathogens were identified in the oral cavity of sharks involved in attacks in recife, and were subjected to antibiotic susceptibility tests using the standardized disc-diffusion method. the maj ... | 2010 | 20413619 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o12. | the o-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o12. its structure was studied by sugar analysis using glc of the alditol acetates and (s)-2-octyl glycosides, methylation analysis, smith degradation, and (1)h and (13)c nmr spectroscopy, including 2d (1)h-(1)h cosy, tocsy, roesy, (1)h-(13)c hsqc, and hmbc experiments. it was found that the polymer is a neutral heteropolysaccharide and has a branched heptasaccharide repeating unit w ... | 2010 | 20471004 |
| female and male genetic contributions to post-mating immune defence in female drosophila melanogaster. | post-mating reduction in immune defence is common in female insects, and a trade-off between mating and immunity could affect the evolution of immunity. in this work, we tested the capacity of virgin and mated female drosophila melanogaster to defend against infection by four bacterial pathogens. we found that female d. melanogaster suffer post-mating immunosuppression in a pathogen-dependent manner. the effect of mating was seen after infection with two bacterial pathogens (providencia rettgeri ... | 2010 | 20573620 |
| enterocyte-like caco-2 cells as a model for in vitro studies of diarrhoeagenic providencia alcalifaciens invasion. | the entry of providencia alcalifaciens into the enterocyte-like cell line caco-2 compared to hep-2 was studied. of the 22 p. alcalifaciens strains, 13 and 21 were invasive for caco-2 and hep-2 cells, respectively. in contrast to hep-2 cells, p. alcalifaciens was internalised by caco-2 cells via receptor-mediated endocytosis. tyrosine kinases play an important role in p. alcalifaciens uptake, also microfilaments and microtubules are engaged in this process. inhibition of endosome acidification by ... | 2010 | 20600792 |
| phylogenetic analysis of the genera proteus, morganella and providencia by comparison of rpob sequences of type and clinical strains suggests the classification of proteus myxofaciens in a new genus cosenzaea gen. nov., as cosenzaea myxofaciens comb. nov. | phylogenetic analysis of partial rpob gene sequences of type and clinical strains belonging to different 16s rrna gene-fingerprinting ribogroups within 11 species of enterobacteria of the genera proteus, morganella, and providencia allowed to define rpob clades supported by high bootstrap values and confirmed by >/=2.5% nucleotide divergence. no rpob clade included strains belonging to different species and the majority of the species were confirmed as discrete and homogeneous, since they consti ... | 2010 | 20709916 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o60. | an o-polysaccharide (o-antigen) was isolated by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o60 and studied by sugar and methylation analyses as well as (1)h and (13)c nmr spectroscopy, including 2d roesy and (1)h,(13)c hmbc experiments in d(2)o and a roesy experiment in a 9:1 h(2)o-d(2)o mixture to reveal correlations for nh protons. it was found that the polysaccharide is built up of linear pentasaccharide repeating units containing an amide of d-glucuronic aci ... | 2010 | 21146157 |
| elucidation of the full o-polysaccharide structure and identification of the core type of the lipopolysaccharide of providencia alcalifaciens o9. | opportunistic human pathogens of the genus providencia from the family enterobacteriaceae are serotyped by their o-antigens, which represent the o-polysaccharide chains of the lipopolysaccharides (lpss) on the cell surface. in this work, the o-polysaccharide of providencia alcalifaciens o9 was obtained by mild acid degradation of a long-chain s-form lps. the structure of the hexasaccharide repeat (o-unit) of the o-polysaccharide containing one d-gal, two d-glc, and three d-galnac residues was es ... | 2011 | 21316039 |
| [the microflora associated with anatomical embalming as a harmful factor of a working process]. | the paper describes the first attempt to isolate and identify the microbiocenosis associated with the objects of a morphological laboratory that uses formalin. the study identified the following microorganisms: the bacteria bacillus sp., providencia alcalifaciens, and staphylococcus aureus, the micromycetes scopulariopsis brevicaulis, aspergillus sp., hormodendron sp., and the mites dermatophagoides pterronissimus. there is experimental evidence that these organisms are resistant to formalin. th ... | 2011 | 21598647 |
| structure of the o-polysaccharide of providencia alcalifaciens o25 containing an amide of d-galacturonic acid with n(ε)-[(r)-1-carboxyethyl]-l-lysine. | an acidic o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o25 followed by gel-permeation and anion-exchange chromatography. the o-polysaccharide was studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including two-dimensional correlation (1)h,(13)c hmbc, and (1)h,(1)h roesy experiments both in d2o and, to detect correlations for nh protons, in a 9 : 1 h2o/d2o mixture. an amino acid was isolated from ... | 2011 | 21639852 |
| structure of the o-polysaccharide from the lipopolysaccharide of providencia alcalifaciens o48. | an o-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of providencia alcalifaciens o48 and studied by sugar and methylation analyses along with (1)h and (13)c nmr spectroscopy, including 2d cosy, tocsy, roesy and (1)h,(13)c hsqc and hmbc experiments. it was found that the polysaccharide is acidic and has a linear mono-o-acetylated tetrasaccharide repeating unit with the following structure: | 2011 | 22099381 |
| Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O28. | An O-polysaccharide and oligosaccharides were isolated by GPC following mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O28. The O-polysaccharide was studied by sugar and methylation analyses, (1)H and (13)C NMR spectroscopy, including 2D ROESY and H-detected (1)H,(13)C HSQC and HMBC experiments, and the following structure of the branched pentasaccharide repeating unit was established: [see formula in text]. This structure was confirmed by ESI MS of the isolated tri ... | 2011 | 21978611 |
| studies on water quality and pathogenic bacteria in coastal water langkawi, malaysia. | a study on physico-chemical parameters and pathogenic bacterial community was carried out at the coastal waters of pulau tuba island, langkawi. the physico-chemical parameters such as temperature (27.43-28.88 degrees c), dissolved oxygen (3.79-6.49 mg l(-1)), ph (7.72-8.20), salinity (33.10-33.96 ppt), total dissolved solids (32.27-32.77 g l(-1)) and specific conductivity (49.83-51.63 ms cm(-1)) were observed. station 3 and station 4 showed highest amount of nitrates (26.93 and 14.61 microg at n ... | 2012 | 23360015 |
| molecular structure of an n-formyltransferase from providencia alcalifaciens o30. | the existence of n-formylated sugars in the o-antigens of gram-negative bacteria has been known since the middle 1980s, but only recently have the biosynthetic pathways for their production been reported. in these pathways, glucose-1-phosphate is first activated by attachment to a dtmp moiety. this step is followed by a dehydration reaction and an amination. the last step in these pathways is catalyzed by n-formyltransferases that utilize n(10) -formyltetrahydrofolate as the carbon source. here ... | 2015 | 25752909 |
| new role for the ankyrin repeat revealed by a study of the n-formyltransferase from providencia alcalifaciens. | n-formylated sugars such as 3,6-dideoxy-3-formamido-d-glucose (qui3nfo) have been observed on the lipopolysaccharides of various pathogenic bacteria, including providencia alcalifaciens, a known cause of gastroenteritis. these unusual carbohydrates are synthesized in vivo as dtdp-linked sugars. the biosynthetic pathway for the production of dtdp-qui3nfo requires five enzymes with the last step catalyzed by an n-formyltransferase that utilizes n(10)-tetrahydrofolate as a cofactor. here we describ ... | 2015 | 25574689 |
| molecular characterizations of cytolethal distending toxin produced by providencia alcalifaciens strains isolated from patients with diarrhea. | cytolethal distending toxins (cdts), which block eukaryotic cell proliferation by acting as inhibitory cyclomodulins, are produced by diverse groups of gram-negative bacteria. active cdt is composed of three polypeptides--cdta, cdtb, and cdtc--encoded by the genes cdta, cdtb, and cdtc, respectively. we developed a pcr-restriction fragment length polymorphism assay for the detection and differentiation of five alleles of cdtb (cdt-i through cdt-v) in escherichia coli and used the assay to investi ... | 2012 | 22252871 |
| first report of a foodborne providencia alcalifaciens outbreak in kenya. | providencia alcalifaciens is an emerging bacterial pathogen known to cause acute gastroenteritis in children and travelers. in july 2013, p. alcalifaciens was isolated from four children appearing for diarrhea at kiambu district hospital (kdh) in kenya. this study describes the outbreak investigation, which aimed to identify the source and mechanisms of infection. we identified seven primary and four secondary cases. among primary cases were four mothers who had children and experienced mild dia ... | 2015 | 26123962 |