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sperm quality and its freezing ability throughout the spawning season in prochilodus lineatus and brycon orbignyanus.the aim of this study was to determine fresh and frozen sperm quality evaluated over two spawning seasons (2013-2014; 2014-2015) in prochilodus lineatus and brycon orbignyanus. the spawning seasons were divided into two sampling periods: november to december and january to february. males were hand-stripped after carp pituitary treatment. fresh sperm motility rate, velocities (curvilinear = vcl; straight-line = vsl; average path = vap), and the beat cross frequency (bcf) were determined using a ...201728166981
structural analysis of fertilization in the fish brycon orbignyanus.in the present work, we analyzed the structure of oocytes and fertilized eggs of the piracanjuba fish (brycon orbignyanus) under light and scanning electron microscopy. after inducing spawning, samples were collected at the moment of oocyte extrusion, when oocytes and semen were mixed (time 0), as well as at 10, 20 and 30 s after mixing, every minute up to 10 min, and then at 15 and 20 min. the oocytes are spherical, translucent and greenish with a mean diameter of 1.3 +/- 0.11 mm. during the ex ...200919032803
molecular characterization and phylogenetic relationships among species of the genus brycon (characiformes: characidae) from four hydrographic basins in brazil.brycon is one of the main genera of neotropical freshwater fish. in brazil, brycon species have been found in many hydrographic basins, such as the amazon, paraná, paraguay, and araguaia-tocantins basins. we examined the phylogenetic relationships among the species brycon orbignyanus, b. hilarii, b. cf. pesu, b. cephalus, b. falcatus, and b. gouldingi, using mitochondrial and nuclear molecular markers. specimens of b. orbignyanus were collected in the paraná river. specimens of b. hilarii were c ...201020449799
the influence of triiodothyronine (t3) on the early development of piracanjuba (brycon orbignyanus).this paper reports the triiodothyronine's (t3) effects on the early growth and survival of piracanjuba (brycon orbignyanus) produced from fertilized eggs hormone exposed. the study was carried out in two phases. in the first phase, eggs divided in 6 batches were immersed in t3 solutions: 0.01; 0.05; 0.1; 0.5 ppm; 1 ppm and control (no t3). after a 15-min immersion, eggs were transferred to incubators where larvae were kept up to 72 h after hatching. larval weight, length and yolk sac volume were ...201020559721
spermatic abnormalities of piracanjuba brycon orbignyanus (valenciennes, 1849) after cryopreservation.the objective of this research was to verify the presence of spermatic abnormalities on semen of brycon orbignyanus after cryopreservation. semen was collected from ten four-year-old males who presented secondary reproductive characteristics for migrating fish. sperm was evaluated for motility, vigor and spermatic morphology before and after cryopreservation. a cryoprotectant solution was made of 20 ml of yolk egg, 5.0 g of glucose and dimethyl sulfoxide diluted in distilled water (10 ml: 90 ml) ...201121881793
milt cryopreservation for rheophilic fish threatened by extinction in the rio grande, brazil.specific protocols for milt cryopreservation have been established for some freshwater fish species. however, cryopreservation reduces sperm quality, giving unsatisfactory results in reproduction.201424872162
complete mitochondrial genome sequence of brycon orbignyanus (characiformes, bryconidae).we report the whole mitochondrial genome of the brycon orbignyanus, commonly known as the piracanjuba. the mitogenome was determined to be a circular, 16,800 bp dna molecule, containing 13 protein-coding genes (pcgs), 2 rrna genes, 22 trna genes and one 1160 bp noncoding control region. twelve of the pcgs were located on the heavy strand, and one pcg (nd6) was located on the light strand. the most common start codon was atg; however, the cox1 gene displayed the gtg start codon. seven pcgs had in ...201625329263
methyl glycol, methanol and dmso effects on post-thaw motility, velocities, membrane integrity and mitochondrial function of brycon orbignyanus and prochilodus lineatus (characiformes) sperm.the aim of this study was to use more accurate techniques to investigate the effects of cryoprotectants (cpas) and extenders on post-thaw sperm quality of brycon orbignyanus and prochilodus lineatus. six freezing media comprising the combination of three cpas (dmso, methanol and methyl glycol) and two extenders (bts and glucose) were used. sperm was diluted in each medium, loaded into 0.5-ml straws, frozen in a nitrogen vapor vessel (dry-shipper), and stored in liquid nitrogen at -196 °c. post-t ...201525433690
water quality and zooplankton in tanks with larvae of brycon orbignyanus (valenciennes, 1949).due to the importance of water variables conditions and available food in the development and survival of fish larvae, the current research evaluates the effects of two different food treatments (ration + zooplankton and only zooplankton) and water quality in tanks with brycon orbignyanus larvae. total water transparency (45 cm) has been mainly associated with short residence time, continuous water flow and shallowness. dissolved oxygen ranged between 1.32 and 7.00 mg.l(-1) in tanks with ration ...200818470380
morphological, cytochemical, and ultrastructural study of thrombocytes and leukocytes in neotropical fish, brycon orbignyanus valenciennes, 1850 (characidae, bryconinae).morphological, cytochemical and ultrastructural studies are important to demonstrate the function of the blood cells, which is very little understood in teleosts. in peripheral blood of 'piracanjuba' brycon orbignyanus, thrombocytes, lymphocytes, monocytes, neutrophils and heterophils were studied and characterized. thrombocytes had a fusiform or oval shape with pas-positive granules. lymphocytes presented small size with sparse basophilic cytoplasm. monocytes were large in size, presented basop ...201517784650
digestive proteinases of brycon orbignyanus (characidae, teleostei): characteristics and effects of protein quality.juvenile piracanjuba, brycon orbignyanus, in the wild consume protein from both plant and animal sources. digestion of protein in piracanjuba begins in the stomach with pepsin, at low ph, and is followed by hydrolysis at alkaline ph in the lumen of the intestine. the digestive system in piracanjuba was evaluated to characterize the enzymes responsible for the digestion of feed protein and their composition. the gastric tissue synthesizes pepsin and the intestine tissues trypsin and chymotrypsin. ...200212031459
optimization of artificial propagation in piracanjuba fish brycon orbignyanus using cryopreserved semen.background: cryopreserved semen could facilitate procedures during the artificial reproduction in fish. factors affecting cryopreservation efficiency are important to define efficient protocols.201627925000
isolation and characterization of flavobacterium columnare (bernardet et al. 2002) from four tropical fish species in brazil.flavobacterium columnare is the causative agent of columnaris disease in freshwater fish, implicated in skin and gill disease, often causing high mortality. the aim of this study was the isolation and characterization of flavobacterium columnare in tropical fish in brazil. piracanjuba (brycon orbignyanus), pacu (piaractus mesopotamicus), tambaqui (colossoma macropomum) and cascudo (hypostomus plecostomus) were examined for external lesions showing signs of colunmaris disease such as greyish whit ...200818660972
physiological and biochemical changes of females of piracanjuba, subjected to induced reproduction.the aim of this experiment was to compare the level of fish female stress during induced reproduction with pituitary extract by two different methods, natural and semiextruded. the reproductive efficiency was 62.5% in the seminatural treatment and 100% in the extruded. obtained egg volume was 5200 ml and 4000 ml, for seminatural and extruded treatments respectively. the mean number of eggs was 46.7 for the seminatural and 52.0 and for the extruded treatment. the percentage of viable eggs was, re ...201626719107
hatching, survival and deformities of piracanjuba (brycon orbignyanus) embryos subjected to different cooling protocols.groups of one hundred brycon orbignyanus embryos at the stage of blastopore closure were subjected to different cooling protocols. different combinations and concentrations of cryoprotectants were tested: sucrose, methanol, ethylene glycol and dimethyl sulfoxide (me2so); at different temperatures (0.0±2.0°c and 8.0±2.0°c) and refrigeration times (6, 10, 24, 72 and 168 h), with the exception of the positive control (incubation without previous cooling). at the end of each refrigeration time, the ...201425445460
a revision of the cis-andean species of the genus <i>brycon</i> müller &amp; troschel (characiformes: characidae).a revision of the cis-andean species of brycon, with the exception of the brycon pesu species-complex, is presented. twenty-one brycon species (including b. pesu) are recognized from cis-andean river systems: brycon stolzmanni steindachner, from the upper río marañon basin, peru; brycon coxeyi fowler, from the río marañon basin, ecuador and peru; brycon polylepis moscó morales, from the lago de maracaibo, río orinoco, upper rio amazonas, and rio tocantins basins, venezuela, colombia, peru, and b ...201728187640
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