| two novel psychrotolerant species, bacillus psychrotolerans sp. nov. and bacillus psychrodurans sp. nov., which contain ornithine in their cell walls. | eleven psychrotolerant bacillus strains with ornithine as diamino acid in position 3 of the peptide side chain of the cell wall and a g+c range of 35.7-38.4 mol% were characterized taxonomically. dna-dna hybridization studies confirmed previously physiologically established groups. high dna-binding values (> 70%) were found within groups i a (consisting of the type strain of bacillus insolitus dsm 5(t) and bacillus insolitus dsm 2272), i b (consisting of isolates 3h1(t0, 71h1, 84e1, 87h2 and 4h2 ... | 2002 | 12508879 |
| marine bacteria associated with the korean brown alga, undaria pinnatifida. | several marine bacterial strains were isolated from undaria pinnatifida (miyok in korean). sixty-six strains were isolated on r2a agar media at 10 degrees and identified by a phylogenetic analysis of the 16s rrna gene sequences. they were grouped into 10 different sequence types based on the initial sequence analysis of the 5' domain of the gene (approximately 500 bp). full sequences of 16s rrna gene were obtained from one strain in each sequence type and the species-affiliation was determined u ... | 2006 | 17205052 |
| recombinant dna-methyltransferase m1.bspaci from bacillus psychrodurans ac: purification and properties. | a restriction-modification system from bacillus psychrodurans ac (recognition sequence 5'-ccgc-3') comprises two dna methyltransferases: m1.bspaci and m2.bspaci. the bspacim1 gene was cloned in the pjw2 vector and expressed in escherichia coli cells. high-purity m1.bspaci preparation has been obtained by chromatography on different carriers. m1.bspaci has a temperature optimum of 30°c and demonstrates maximum activity at ph 8.0. m1.bspaci modifies the first cytosine in the recognition sequence 5 ... | 2010 | 21314619 |
| diversity of bacteria and archaea from a landfill in chandigarh, india as revealed by culture-dependent and culture-independent molecular approaches. | the bacterial community structure of a municipal landfill in chandigarh, india was analysed by culture-dependent as well as culture-independent molecular approaches, and archaeal structure by the latter method. samples were collected in two phases from the surface and a depth of 0.91 m in june, 2004 and from 0.91 m, 1.52 m and 1.68 m in may, 2005. after serial dilutions, samples were plated onto tryptic soy agar (tsa), plate count agar (pca), tryptic soy broth agar (tsba) and tsba100 (tsba dilut ... | 2012 | 23274043 |