Publications

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cloning and sequencing of columbid circovirus (cocv), a new circovirus from pigeons.the complete nucleotide sequence of columbid circovirus (cocv) isolated from pigeons is described. cocv was amplified using a consensus primer pcr approach directed against conserved sequences within the rep genes of vertebrate circoviruses. the genome of cocv is circular and 2037 nt in size. it displays 55% homology to the genome of psittacine beak and feather disease virus and is more distantly related (< 40% homology) to porcine circovirus type 1 and 2. two major open reading frames were iden ...200011205099
nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus circovirus of the family circoviridae.the circular, single-stranded dna genome of a novel circovirus of canaries, tentatively named canary circovirus (cacv), was cloned and sequenced. sequence analysis indicated that the genome was 1952 nucleotides (nt) in size and had the potential to encode three viral proteins, including the putative capsid and replication-associated (rep) proteins. the cacv genome shared greatest sequence similarity (58.3% nt identity) with the newly characterized columbid circovirus (cocv) and was more distantl ...200111602793
a method to diagnose pigeon circovirus infection in vivo.this study describes a method to detect in vivo pigeon circovirus (picv, also called columbid circovirus, cocv) identified recently. blood samples of healthy and diseased pigeons were investigated. dna was isolated from a drop of blood spotted and dried on filter paper. a nested pcr using two primer pairs from the cap gene was performed to detect picv. using this method, picv nucleic acid was detected in 17 of 53 pigeons examined. pcr using dna from dried blood samples is a simple, fast and reli ...200212020792
detection of pigeon circovirus by polymerase chain reaction.pigeon circovirus was identified by polymerase chain reaction (pcr) in young pigeons belonging to 12 different lofts. viral dna was extracted from formalin-fed, paraffin-imbedded tissues containing primarily bursa and occasionally liver and spleen with a commercial kit. pcr primers were selected from a published sequence for columbid circovirus and evaluated in a pcr assay. the histopathologic examination of various tissues revealed basophilic globular intracytoplasmic inclusions in the mononucl ...200312713182
a diagnostic study on columbid circovirus infection.a systematic study was performed to examine the frequency of columbid circovirus (cocv) infection in diseased young pigeons submitted for necropsy and its relevance to pigeon health. existing diagnostic methods were compared. among 176 diseased young pigeons examined, cocv infection was detected in 83 cases using negative contrast electron microscopy. histopathological examination allowed a clear diagnosis in only 42 pigeons. therefore, a polymerase chain reaction assay and an in situ hybridizat ...200119184954
cloning and expression of a truncated pigeon circovirus capsid protein suitable for antibody detection in infected pigeons.infections with pigeon circovirus (picv) (also termed columbid circovirus) occur in meat and racing pigeons (columba livia) of all ages and have been reported worldwide. a picv infection is associated with immunosuppression and the development of young pigeon disease syndrome. an indirect enzyme-linked immunosorbent assay (elisa) for the detection of virus-specific serum antibody was developed for research purposes. in the absence of a method to propagate picv in cell culture, the assay was base ...200919322712
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