| an emergent poxvirus from humans and cattle in rio de janeiro state: cantagalo virus may derive from brazilian smallpox vaccine. | the biological properties of poxvirus isolates from skin lesions on dairy cows and milkers during recent exanthem episodes in cantagalo county, rio de janeiro state, brazil, were more like vaccinia virus (vv) than cowpox virus. pcr amplification of the hemagglutinin (ha) gene substantiated the isolate classification as an old world orthopoxvirus, and alignment of the ha sequences with those of other orthopoxviruses indicated that all the isolates represented a single strain of vv, which we have ... | 2000 | 11080491 |
| araçatuba virus: a vaccinialike virus associated with infection in humans and cattle. | we describe a vaccinialike virus, araçatuba virus, associated with a cowpoxlike outbreak in a dairy herd and a related case of human infection. diagnosis was based on virus growth characteristics, electron microscopy, and molecular biology techniques. molecular characterization of the virus was done by using polymerase chain reaction amplification, cloning, and dna sequencing of conserved orthopoxvirus genes such as the vaccinia growth factor (vgf), thymidine kinase (tk), and hemagglutinin. we u ... | 2003 | 12603984 |
| human vaccinia-like virus outbreaks in são paulo and goiás states, brazil: virus detection, isolation and identification. | since october 2001, the adolfo lutz institute has been receiving vesicular fluids and scab specimens of patients from paraíba valley region in the são paulo and minas gerais states and from são patricio valley, in the goiás state. epidemiological data suggested that the outbreaks were caused by cowpox virus or vaccinia virus. most of the patients are dairy milkers that had vesiculo-pustular lesions on the hands, arms, forearms, and some of them, on the face. virus particles with orthopoxvirus mo ... | 2004 | 15654477 |
| a pcr-based assay for detection of emerging vaccinia-like viruses isolated in brazil. | we report 2 strategies to identify brazilian vaccinia virus (vacv) isolates related to cantagalo virus (ctgv) based on the amplification of the hemagglutinin (ha) gene by the polymerase chain reaction (pcr). one pcr protocol was combined with restriction analysis using the endonuclease snab i, generating a unique digestion pattern for ctgv amplicons. the restriction profile could identify 41 ctgv-related isolates in 43 clinical specimens and clearly differentiated them from other orthopoxviruses ... | 2007 | 16949244 |
| in vitro activity of cidofovir against the emerging cantagalo virus and the smallpox vaccine strain ioc. | the antiviral effect of cidofovir was evaluated against two strains of vaccinia virus: the field strain cantagalo virus (ctgv) and the smallpox vaccine ioc. the drug severely inhibited virus replication, revealing an ec(50) (drug concentration required to inhibit 50% of virus replication) of 7.68 microm and 9.66 microm, respectively, for ctgv and vaccine strain ioc. similarly, other field isolates of cantagalo-like viruses recently collected in distinct outbreaks were equally sensitive to the dr ... | 2009 | 18804965 |
| antiviral evaluation of n-amino-1,2,3-triazoles against cantagalo virus replication in cell culture. | this paper describes the antiviral evaluation of new n-amino-1,2,3-triazole derivatives, 1-(substituted-phenylamino)-5-methyl-1h-[1,2,3]-triazole-4-carboxylic acid ethyl esters, 3 and 1-(4-substituted-phenylamino)-5-methyl-1h-[1,2,3]-triazole-4-carboxylic acid hydrazides, 4, on cantagalo virus replication. 1-(4-fluoro-phenylamino)-5-methyl-1h-[1,2,3]-triazole-4-carboxylic acid hydrazide, 4e, exhibited a significant antiviral effect. characterization of all compounds was confirmed by ir, (1)h and ... | 2009 | 19481841 |
| spread of cantagalo virus to northern brazil. | | 2009 | 19624947 |
| cidofovir inhibits genome encapsidation and affects morphogenesis during the replication of vaccinia virus. | cidofovir (cdv) is one of the most effective antiorthopoxvirus drugs, and it is widely accepted that viral dna replication is the main target of its activity. in the present study, we report a detailed analysis of cdv effects on the replicative cycles of distinct vaccinia virus (vacv) strains: cantagalo virus, vacv-ioc, and vacv-wr. we show that despite the approximately 90% inhibition of production of virus progeny, virus dna accumulation was reduced only 30%, and late gene expression and genom ... | 2009 | 19726515 |
| potent antiviral activity of brequinar against the emerging cantagalo virus in cell culture. | in the present work, the antiviral activity of brequinar (bqr) against the replication of cantagalo virus was evaluated. bqr is a potent inhibitor of cellular dihydroorotate dehydrogenase, an enzyme of the de novo pyrimidine biosynthetic pathway. infection in the presence of 0.5μm bqr reduced virus progeny production by >90%, revealing an ec(50) (drug concentration required to inhibit 50% of virus replication) of 0.017μm. replication of other orthopoxviruses was also inhibited by bqr at similar ... | 2011 | 21840180 |
| one-step duplex polymerase chain reaction for the detection of swinepox and vaccinia viruses in skin lesions of swine with poxvirus-related disease. | infection of pigs with swinepox virus (swpv) was reported in brazil in 2011. swpv causes a systemic pustular disease in pigs and the symptoms are clinically indistinguishable from those caused by vaccinia virus (vacv) infection. pigs infected with vacv have been reported in various countries; however, vacv is endemic in brazil, india and other countries, where it affects mainly dairy cows, dairy buffaloes and dairy workers causing localized pustules. the transmission of vacv to other susceptible ... | 2015 | 25804245 |
| presence of neutralizing antibodies to orthopoxvirus in capybaras (hydrochoerus hydrochaeris) in brazil. | cantagalo virus is a strain of vaccinia virus (genus orthopoxvirus) and the etiological agent of an important vesicopustular disease that affects dairy cows and milkers in brazil. the reservoirs involved in the maintenance of this virus in nature are unknown. in the present work, the detection of neutralizing antibodies to orthopoxvirus in capybaras collected in são paulo state is reported. capybaras are the largest rodent species native to south america and have already been reported as putativ ... | 2014 | 25500667 |
| myd88-dependent immunity to a natural model of vaccinia virus infection does not involve toll-like receptor 2. | although the pattern recognition receptor toll-like receptor 2 (tlr2) is typically thought to recognize bacterial components, it has been described to alter the induction of both innate and adaptive immunity to a number of viruses, including vaccinia virus (vacv). however, many pathogens that reportedly encode tlr2 agonists may actually be artifactually contaminated during preparation, possibly with cellular debris or merely with molecules that sensitize cells to be activated by authentic tlr2 a ... | 2014 | 24403581 |
| increased susceptibility of cantagalo virus to the antiviral effect of st-246®. | cantagalo virus (ctgv) is the etiologic agent of a pustular disease in dairy cows and dairy workers in brazil with important economical and occupational impacts. nevertheless, no antiviral therapy is currently available. st-246 is a potent inhibitor of orthopoxvirus egress from cells and has proved its efficacy in cell culture and in animal models. in this work, we evaluated the effect of st-246 on ctgv replication. plaque reduction assays indicated that ctgv is 6-38 times more susceptible to th ... | 2013 | 23257396 |