| protective cell-mediated immunity by dna vaccination against papillomavirus l1 capsid protein in the cottontail rabbit papillomavirus model. | papillomavirus major capsid protein l1 has successfully stimulated protective immunity against virus infection by induction of neutralizing antibodies in animal models and in clinical trials. however, the potential impact of l1-induced protective cell-mediated immune (cmi) responses is difficult to measure in vivo because of the coincidence of anti-l1 antibody. in this study, we tested the hypothesis that l1 could activate cmi, using the cottontail rabbit papillomavirus (crpv)-rabbit model. a un ... | 2006 | 16987067 |
| protection of rabbits against challenge with rabbit papillomaviruses by immunization with the n terminus of human papillomavirus type 16 minor capsid antigen l2. | current l1 virus-like particle (vlp) vaccines provide type-restricted protection against a small subset of the human papillomavirus (hpv) genotypes associated with cervical cancer, necessitating continued cytologic screening of vaccinees. cervical cancer is most problematic in countries that lack the resources for screening or highly multivalent hpv vlp vaccines, suggesting the need for a low-cost, broadly protective vaccinogen. here, n-terminal l2 polypeptides comprising residues 1 to 88 or 11 ... | 2007 | 17715230 |
| persistent conjunctival papilloma due to oral papillomavirus infection in a rabbit in new zealand. | a 3-yr-old female flemish giant pet rabbit developed a papilloma on the right nictitating membrane. although the papilloma was excised surgically, it promptly recurred. examination of the eye 10 wk after surgery revealed that in addition to the initial mass, 2 smaller papillomas were present on the lower eyelid. all 3 masses were excised, and histology revealed papillomatous hyperplasia of the conjunctival epithelium, koilocytosis, and intranuclear viral inclusions. polymerase chain reaction amp ... | 2007 | 17877332 |
| persistence of viral dna in the epithelial basal layer suggests a model for papillomavirus latency following immune regression. | rabbit oral papillomavirus (ropv) causes benign and spontaneously regressing oral lesions in rabbits, and is a useful model of disease associated with low-risk human papillomavirus types. here we have adapted the ropv system to study papillomavirus latency. following lesion regression, ropv dna persists at the majority of regressed sites at levels substantially lower than those found in productive papillomas. spliced viral transcripts were also detected. ropv persistence in the absence of diseas ... | 2011 | 21492895 |
| protection of rabbits against cutaneous papillomavirus infection using recombinant tobacco mosaic virus containing l2 capsid epitopes. | cottontail rabbit papillomavirus (crpv) and rabbit oral papillomavirus (ropv) represent distantly related, cutaneous and mucosal tissue tropic papillomaviruses respectively that can infect the same host. these two viruses were used to test the effectiveness of an l2 peptide-based vaccine (aa 94-122) that was delivered on the surface of recombinant tobacco mosaic virus (rtmv) particles. groups of nzw rabbits received combinations of crpvl2, ropvl2 and crpv+ropvl2 rtmv vaccines, and were then chal ... | 2006 | 16725236 |
| characterization of immune responses during regression of rabbit oral papillomavirus infections. | rabbit oral papillomavirus (ropv) is a mucosatropic papillomavirus that causes small benign discrete papillomas within the oral cavity of domestic rabbits. the goal of this study was to characterize the immune cell infiltrate over the course of regression of oral papillomas. ropv-infected oral tissues were harvested at various time points after infection and analyzed by immunohistochemistry for papilloma morphology, viral capsid proteins, and associated immune infiltrates. the results of this st ... | 2005 | 16270899 |
| rabbit oral papillomavirus complete genome sequence and immunity following genital infection. | rabbit oral papillomavirus (ropv) infects mucosal tissues of domestic rabbits. the viral genomic sequence has been determined and the most related papillomavirus type was the cutaneous cottontail rabbit papillomavirus (crpv). homologies between the open reading frames (orfs) of ropv and crpv, however, ranged from 68% amino acid identity for l1 to only 23% identity for e4. shared features unique to the two rabbit viruses included a large e6 orf and a small e8 orf that overlapped the e6 orf. serol ... | 2000 | 10753723 |
| life cycle heterogeneity in animal models of human papillomavirus-associated disease. | animal papillomaviruses are widely used as models to study papillomavirus infection in humans despite differences in genome organization and tissue tropism. here, we have investigated the extent to which animal models of papillomavirus infection resemble human disease by comparing the life cycles of 10 different papillomavirus types. three phases in the life cycles of all viruses were apparent using antibodies that distinguish between early events, the onset of viral genome amplification, and th ... | 2002 | 12239317 |
| rabbit genital tissue is susceptible to infection by rabbit oral papillomavirus: an animal model for a genital tissue-targeting papillomavirus. | rabbit oral papillomavirus (ropv) is a mucosatropic papillomavirus which naturally infects oral mucosal sites of domestic rabbits. in this study, we tested the hypothesis that rabbit genital mucosa is also susceptible to ropv infection by using the athymic mouse xenograft system and adult immunocompetent rabbits. subrenal xenografts of ropv-infected rabbit vulvar and penile sheath tissues were strongly positive for ropv infection by histologic, in situ hybridization, and southern analyses. direc ... | 1998 | 9573297 |
| cloning and molecular characterization of an oral papillomavirus of domestic rabbits. | dna obtained from new zealand white rabbit oral papillomas was analyzed for the presence of papillomavirus dna. the viral genome was cloned as three separate subclones, which were each mapped and oriented with respect to one another. comparisons with other papillomavirus dnas by southern blot hybridization under various conditions of stringency revealed a strong area of conservation among the dnas of the rabbit oral papillomavirus (ropv) and crpv, hpv-1a, hpv-16, and bpv-5, but not with 12 other ... | 1988 | 2827380 |
| cloning and characterization of a papillomavirus associated with papillomas and carcinomas in the european harvest mouse (micromys minutus). | individuals in a colony of european harvest mice (micromys minutus) were diagnosed with a variety of skin tumors including papillomas, trichoepitheliomas, and sebaceous carcinomas. papillomavirus group-specific antigens and viruslike particles were detected in the papillomas. a 7.6-kilobase supercoiled circular dna, which was cleaved once by ecori, was visualized in papilloma extracts by low-stringency southern blot hybridization with a bovine papillomavirus type 2 probe. the molecule was cloned ... | 1988 | 2824849 |
| characterization of three rabbit oral papillomavirus oncogenes. | rabbit oral papillomavirus (ropv) induces warts in mucosal tissues, and represents a useful model for understanding host-virus interactions that are reflected in mucosal/hpv infections. ropv induces benign papillomas that regress in 100% of infected rabbits. we previously reported the complete genome sequence of ropv. however, the oncogenic potential of this virus is unknown because of immunologically mediated regression. the purpose of this study was to characterize the transforming proteins of ... | 2004 | 15231385 |
| model systems of human papillomavirus-associated disease. | human papillomaviruses (hpvs) cause a range of serious diseases, including the vast majority of cervical cancers, most anal cancers and around half of head and neck cancers. they are also responsible for troublesome benign epithelial lesions, including genital warts and laryngeal papillomas, and in some individuals hpvs lead to recurrent respiratory papillomatosis and other difficult-to-manage diseases. as a result, there is a great need for model systems that accurately mimic papillomavirus inf ... | 2016 | 26456009 |
| laboratory production of infectious stocks of rabbit oral papillomavirus. | several small, raised lesions from the underside of the tongue of domestic rabbits were isolated, and an extract prepared and tested for the presence of rabbit oral papillomavirus (ropv). two weeks after inoculation of this extract into the underside of rabbit tongues, multiple small discrete, grey-white nodules were observed that reached a maximum size of 2 mm in diameter by 5 weeks. these lesions showed typical ropv pathology, and nuclei stained positive for papillomavirus (pv) group-specific ... | 1996 | 8760428 |