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configurational prey selection by bufo, alytes, bombina and hyla.the aim of the present study is to answer the following questions; (1) is the ability of toads to distinguish between different configurational prey stimuli based on learning during ontogeny? (2) is the configurational meaning of a stimulus influenced by its distance from the animal? (3) are there differences in configurational prey selection among different anuran species? (1) experiments with postmetamorphic juveniles of alytes obstetricans indicate that preferences for worm-like objects can b ...1979114268
comparative studies on the vascular organization of carotid labyrinths of anurans and caudates.the three-dimensional structures of the carotid labyrinth in five species of anurans representing four families (rana nigromaculata, rana catesbeiana, bufo japonicus, hyla arborea, and xenopus laevis), and three species of caudates representing three families (cynops pyrrhogaster, hynobius nebulosus, ambystoma mexicanum) were compared using vascular corrosion castings and scanning electron microscopy (sem). anuran carotid labyrinths are spherical in shape and are classified into two groups accor ...19902338718
identification by immunofluorescence of acth-producing cells in the pituitary gland of the tree frog hyla arborea.the indirect immunofluorescence procedure was used to localize acth-producing cells in the pituitary distal lobe from hyla arborea adult specimens; the antiserum employed was rabbit anti-synthetic acth (1-24) conjugated with bsa. immunohistochemical staining was suppressed by solid-phase absorption of the anti-acth with the specific antigen. in the distal lobe one cell population is immunoreactive; these cells are predominantly localized in the anterior half, both ventrally and dorsally; they ex ...19852991078
comparative studies on the electric nature of amphibian gonadotropin.the isoelectric focusing (ief) profiles of pituitary gonadotropic (gth) activity in six species of adult male amphibians were examined in addition to the newt and bullfrog. the gth activity was measured by two radioreceptor assay (rra) systems; one with xenopus laevis testicular homogenate as the receptor preparation, and the other with anolis carolinensis testicular homogenate, both employing 125i-labeled rat fsh as the radioligand, or by a radioimmunoassay (ria) system for bullfrog basic gth ( ...19853874809
tubular myelin structures in the lungs of amphibia. the mode of formation.the lungs of the tree frog hyla arborea and the toad bufo bufo were fixed in glutaraldehyde with the addition of picric acid or tannic acid to visualize tubular myelin (tm), one of the forms of pulmonary surfactant. the lungs of both hyla and bufo are complex in structure and possess one type of pneumocytes containing numerous lamellar bodies (lbs). the alveolar lining layer in the lung of hyla includes numerous lbs at different stages of transformation into a monolayer film, but the lattice is ...19846421587
vasoactive intestinal polypeptide (vip)-like immunoreactivity in anuran intestine.immunocytochemical and radioimmunological techniques with region specific antisera have been used to identify a vasoactive intestinal polypeptide-like material in the anuran intestine. seven species of anura were investigated: bombina bombina, alytes obstetricans, rana temporaria, rana esculenta, hyla arborea, hyla crepitans and bufo bufo. in five of the species (a. obstetricans, r. temporaria, h. arborea, h. crepitans and b. bufo) vasoactive intestinal polypeptide-like immunoreactive mucosal en ...19816784927
[the morphology of amphibian skins: comparison between some species in different natural habitats].a comparison in the morphology of the skin in different species of amphibians has been performed in this note. species examined had different natural habitat: some of them were closely dependent on a watery environment (proteus anguinus, triturus cristatus, rana esculenta and r. graeca), whereas others were more or less adapted to life on land (rana dalmatina, salamandra salamandra, bufo bufo, b. viridis and hyla arborea). structural patterns of both epidermis and derm varied from species to spe ...19827115576
lectin binding patterns in amphibian skin epithelium.seven lectins (pna, dba, wga, uea-i, rca, sba, con a) were used to localize glycoconjugates in the skin of 10 species of amphibia, 7 anurans (bufo marinus, bufo bufo, rana ridibunda, rana pipiens, hyla arborea, pelobates syriacus and xenopus laevis) and 3 urodeles (salamandra salamandra, triturus vulgaris and ambystoma mexicanum). it was found that every lectin has a specific binding pattern in the skin of each species. no common pattern could be established, either among frogs or toads, nor for ...199910443294
pattern and rate of ovary differentiation with reference to somatic development in anuran amphibians.the rate of somatic development of anuran amphibians is only roughly correlated with the rate of gonad differentiation and varies among species. the somatic stage of a tadpole often does not reflect its age, which seems to be crucial for gonad differentiation rate. we compared the morphology and differentiation of developing ovaries at the light and electron microscopy level, with reference to somatic growth and age of a female. our observations were performed on 12 species of six families (rana ...200414666524
impact of ammonium nitrate on growth and survival of six european amphibians.we conducted static experiments to assess the effects of ammonium nitrate fertilizer on embryos and larvae of six european amphibians: sharp-ribbed salamander (pleurodeles waltl), iberian painted frog (discoglossus galganoi), western spadefoot toad (pelobates cultripes), common toad (bufo bufo), natterjack toad (bufo calamita), and common tree frog (hyla arborea). embryos were exposed to different and environmentally relevant concentrations of ammonium nitrate (0 to 200 mg no(3)(-)/l) for 15 day ...200415386149
a sex-specific marker reveals male heterogamety in european tree frogs.most amphibians examined so far show undifferentiated sex chromosomes. the heterogametic sex's identity, usually revealed through indirect means, often varies among closely related species or even populations (as do sex-linkage groups), suggesting great evolutionary instability of the sex-determining genes. here we take advantage of a sex-specific marker that amplifies in several related species of european tree frogs (hyla arborea group) to disclose a homogeneous pattern of male heterogamety. b ...200616581942
a metazoan parasitological research of some iraqi amphibians.the incidence and intensity of metazoan parasites in 3 species of iraqi amphibians were studied. the amphibians were rana ridibunda, bufo viridis and hyla arborea. twenty-four species of helminths were encountered, including 16 trematodes, 1 cestode and 7 nematodes. their respective names are: polystoma integerrimum, prosotocus confusus, p. fuelleborni, pleurogenoides gastroporus, p. medians, sonsinotrema tacapense, opisthioglyphe ranae, haplometra cylindracea, haematoloechus volgensis, h. vitel ...200718224630
extreme heterochiasmy and nascent sex chromosomes in european tree frogs.we investigated sex-specific recombination rates in hyla arborea, a species with nascent sex chromosomes and male heterogamety. twenty microsatellites were clustered into six linkage groups, all showing suppressed or very low recombination in males. seven markers were sex linked, none of them showing any sign of recombination in males (r=0.00 versus 0.43 on average in females). this opposes classical models of sex chromosome evolution, which envision an initially small differential segment that ...200818426748
the development and fine structure of ultimobranchial glands in larval anurans. ii. bufo viridis, hyla arborea, and rana ridibunda.a comparative optical microscopic and ultrastructural study on the ultimobranchial (ub) glands of three common species of israeli anurans: bufo viridis, hyla arborea, and rana ridibunda during metamorphosis is presented. the ub glands typically consist of a single follicle with a central lumen, though occasionally secondary follicles are present in hyla and rana. a single ub cell type is found which appears either in a very electron-dense "dark" form or as a less dense "light" form, though the r ...20051201602
[intra-and interspecific crowding-effects in tadpoles ofbufo calamila laur.]tadpoles ofbufo calamita, a species that spawns in little temporary pools, were tested on intra- and interspecific crowding-effects. water was conditioned by tadpoles of the following species:bufo calamita (tadpoles of different age),alytes obstetricans, bombina variegata, bufo bufo, hyla arborea, rana lessonae, r. ridibunda andr. temporaria. tadpoles ofbufo calamita are most sensitive to water conditioned by older tadpoles of the own species during the first 2-3 weeks of development. they are a ...197228307074
 tadpoles of rana temporaria which have been raised in an artificial pond prey in the experimental situation shown in fig. 1 upon the spawn of the following sympatric european anura species: rana temporaria (cannibalism), rana esculenta, rana ridibunda, bufo bufo, bufo calamita, bombina variegata and hyla arborea (predation). - rana temporaria is an early breeder. thus selection pressure acts in the following ways: rana temporaria establishes for itself an explosive breeding pattern. species whic ...197028309036
non-invasive measurement of frog skin reflectivity in high spatial resolution using a dual hyperspectral approach.most spectral data for the amphibian integument are limited to the visible spectrum of light and have been collected using point measurements with low spatial resolution. in the present study a dual camera setup consisting of two push broom hyperspectral imaging systems was employed, which produces reflectance images between 400 and 2500 nm with high spectral and spatial resolution and a high dynamic range.201324058464
anuran amphibia which are not acclimable to high salt, tolerate high plasma urea.1. the capacity of five anuran amphibians (bufo viridis, b. regularis, rana ridibunda, hyla arborea and pelobates syriacus) to acclimate to nacl and urea solutions was investigated. 2. all species could be acclimated to relatively high concentrations of urea solutions, while only bufo viridis and hyla arborea could be acclimated to 500 mosm/kg or higher nacl solutions. 3. the plasma urea concentration in b. viridis and h. arborea was elevated to levels over 140 mmol/l. 4. the sum of plasma sodiu ...19921358505
a comparative ultrastructural study on ultimobranchial glands of some israeli anurans (bufo viridis, rana ridibunda and hyla arborea). 19724339731
studies on the sensitivity of the iris of amphibians (hyla arborea and bufo viridis) to epinephrine and norepinephrine. 19695795817
sex reversal assessments reveal different vulnerability to endocrine disruption between deeply diverged anuran lineages.multiple anthropogenic stressors cause worldwide amphibian declines. among several poorly investigated causes is global pollution of aquatic ecosystems with endocrine disrupting compounds (edcs). these substances interfere with the endocrine system and can affect the sexual development of vertebrates including amphibians. we test the susceptibility to an environmentally relevant contraceptive, the artificial estrogen 17α-ethinylestradiol (ee2), simultaneously in three deeply divergent systematic ...201627029458
the plasticizer bisphenol a affects somatic and sexual development, but differently in pipid, hylid and bufonid anurans.due to their terrestrial habitats and aquatic reproduction, many amphibians are both very vulnerable and highly suitable bioindicators. the plasticizer bisphenol a (bpa) is one of the most produced chemical substances worldwide, and knowledge on its impacts on humans and animals is mounting. bpa is used for the industrial production of polycarbonate plastics and epoxy resins and found in a multitude of consumer products. studies on bpa have involved mammals, fish and the fully aquatic anuran mod ...201627285164
doping for sex: bad for mitochondrial performances? case of testosterone supplemented hyla arborea during the courtship period.sexual selection has been widely explored from numerous perspectives, including behavior, ecology, and to a lesser extent, energetics. hormones, and specifically androgens such as testosterone, are known to trigger sexual behaviors. their effects are therefore of interest during the breeding period. our work investigates the effect of testosterone on the relationship between cellular bioenergetics and contractile properties of two skeletal muscles involved in sexual selection in tree frogs. call ...201728478209
using conventional f-statistics to study unconventional sex-chromosome differentiation.species with undifferentiated sex chromosomes emerge as key organisms to understand the astonishing diversity of sex-determination systems. whereas new genomic methods are widening opportunities to study these systems, the difficulty to separately characterize their x and y homologous chromosomes poses limitations. here we demonstrate that two simple f-statistics calculated from sex-linked genotypes, namely the genetic distance (fst) between sexes and the inbreeding coefficient (fis) in the hete ...201728462023
impaired gonadal and somatic development corroborate vulnerability differences to the synthetic estrogen ethinylestradiol among deeply diverged anuran lineages.amphibians are undergoing a global decline. one poorly investigated reason could be the pollution of aquatic habitats by endocrine disrupting compounds (edcs). we tested the susceptibility to the synthetically stabilized estrogen 17α-ethinylestradiol (ee2) in three deeply diverged anuran species, differing in sex determination systems, types of gonadogenesis and larval ecologies. to understand whether data from the amphibian model xenopus laevis (pipidae) are analogous and applicable to only dis ...201627434076
a bactericidal protein in bombina variegata pachypus skin venom.the skin venom of the yellow bellied toad bombina variegata pachypus has an antimicrobial activity which seems to be correlated to the presence of a 6700 mol. wt polypeptide. this polypeptide was purified by electroelution from sds-urea-polyacrylamide gels and characterized for its antimicrobial activity. a bactericidal action was detected at concentrations with little or no cytolytic effect. the determination of the minimal inhibitory concentration showed that there was activity against gram po ...19912048146
nematopsis temporariae (gregarinasina, apicomplexa, alveolata) intracellular infectious agent of tadpole livers.amphibians are in decline as a result of habitat destruction, climate change and infectious diseases. tadpoles are thought susceptible to infections because they are dependent on only an innate immune system (e.g. macrophages). this is because the frog adaptive immune system does not function until later stages of the life cycle. in 1920, nöller described a putative infectious agent of tadpoles named nematopsis temporariae, which he putatively assigned to gregarine protists (apicomplexa). here, ...201627119160
sexual dimorphism of amh, dmrt1 and rspo1 localization in the developing gonads of six anuran species.in vertebrates, several genes which are differentially expressed in various species, have been implicated in sex determination and gonadal differentiation. we used immunolocalization to study the expression pattern of three proteins amh, dmrt1, rspo1 involved in the sexual differentiation of gonads. the pattern of amh, dmrt1 and rspo1 expression was analyzed in x. laevis and in five other divergent anuran species: bombina bombina, bufo viridis, hyla arborea, rana arvalis and rana temporaria duri ...201324623081
retinoic acid homeostasis regulates meiotic entry in developing anuran gonads and in bidder's organ through raldh2 and cyp26b1 proteins.the vitamin a (retinol) and its metabolites such as retinoic acid (ra) affect vertebrate gametogenesis. the level of ra in cells relies on the balance between its synthesis and degradation. the sex-dependent equilibrium is reached in different ways in various species. it is known that ra induces meiosis in developing gonads in mouse, chicken and urodel amphibians, but its role in anuran amphibians has not been studied. here we show in six anuran species (xenopus laevis, bombina bombina, hyla arb ...201624056063
high-density sex-specific linkage maps of a european tree frog (hyla arborea) identify the sex chromosome without information on offspring sex.identifying homology between sex chromosomes of different species is essential to understanding the evolution of sex determination. here, we show that the identity of a homomorphic sex chromosome pair can be established using a linkage map, without information on offspring sex. by comparing sex-specific maps of the european tree frog hyla arborea, we find that the sex chromosome (linkage group 1) shows a threefold difference in marker number between the male and female maps. in contrast, the num ...201626374238
timeframe of speciation inferred from secondary contact zones in the european tree frog radiation (hyla arborea group).hybridization between incipient species is expected to become progressively limited as their genetic divergence increases and reproductive isolation proceeds. amphibian radiations and their secondary contact zones are useful models to infer the timeframes of speciation, but empirical data from natural systems remains extremely scarce. here we follow this approach in the european radiation of tree frogs (hyla arborea group). we investigated a natural hybrid zone between two lineages (hyla arborea ...201526253600
exotic fish in exotic plantations: a multi-scale approach to understand amphibian occurrence in the mediterranean region.globally, amphibian populations are threatened by a diverse range of factors including habitat destruction and alteration. forestry practices have been linked with low diversity and abundance of amphibians. the effect of exotic eucalyptus spp. plantations on amphibian communities has been studied in a number of biodiversity hotspots, but little is known of its impact in the mediterranean region. here, we identify the environmental factors influencing the presence of six species of amphibians (th ...201526061096
evolutionary trend for metamery reduction and gonad shortening in anurans revealed by comparison of gonad development.the gonads develop as the metameric mesodermal structures at the ventral surface of the mesonephroi. to study the evolutionary trends for anuran gonads, we performed comparative analysis of three species from the basal paraphyletic group - archaeobatrachia (bombina bombina, xenopus laevis, pelobates fuscus) and five species from more derived monophyletic neobatrachia group (hyla arborea, bufotes viridis, rana dalmatina, rana arvalis, rana temporaria). light and scanning electron microscopy analy ...201426154333
genetic erosion in wild populations makes resistance to a pathogen more costly.populations that have suffered from genetic erosion are expected to exhibit reduced average trait values or decreased variation in adaptive traits when experiencing periodic or emergent stressors such as infectious disease. genetic erosion may consequentially modify the ability of a potential host population to cope with infectious disease emergence. we experimentally investigate this relationship between genetic variability and host response to exposure to an infectious agent both in terms of s ...201222671558
histochemical studies on the tongue of anuran amphibian: i. mucopolysaccharide histochemistry of the papillae and the lingual glands in hyla arborea l., rana esculenta l. and bufo vulgaris laur. 2002128314
systematic analysis of animals used in medieval azerbaijan medicine.in order to study the special composition of animals used in the medieval medicine of azerbaijan, a wide range of medieval sources on medicine and pharmacognosy from the collection of the institute of manuscripts of the azerbaijan academy of sciences in baku has been studied. about 40 medieval sources from the 10-18th centuries including 17 manuscripts in turkic, persian and arabic have been selected as the objects of this study. as a result, 150 species of animals described in medieval azerbaij ...200617153727
[helminth parasites of hyla arborea (linneaus, 1758) (tree frog) collected from bursa, edirne and sakarya].twenty-seven tree frogs (hyla arborea ) were collected from 3 different regions of turkey from 1995-2000 in order to detect helminths . as a result of our survey, 1 monogenea species and 2 nematoda species were found. these are polystoma skrjabini, cosmocerca ornata and oswaldocruzia filiformis. this is the second time polystoma skrjabini has been recorded for this host, while this is the first time cosmocerca ornata and oswaldocruzia filiformis have been recorded for this host.200617106858
multimodality: a way to cope with road traffic noise? the case of european treefrog (hyla arborea).in the last decades, traffic noise has become a new challenge for efficient animal communication and several studies suggest that it is involved in population declines. although poorly investigated in a traffic noise context, communication is generally multimodal, which can be viewed as a way to improve communication efficiency by allowing shift from a sensory modality to another when one modality suffers from noise. in the present study, we investigated multimodal shift in the european treefrog ...201425151940
density, climate and varying return points: an analysis of long-term population fluctuations in the threatened european tree frog.experimental research has identified many putative agents of amphibian decline, yet the population-level consequences of these agents remain unknown, owing to lack of information on compensatory density dependence in natural populations. here, we investigate the relative importance of intrinsic (density-dependent) and extrinsic (climatic) factors impacting the dynamics of a tree frog (hyla arborea) population over 22 years. a combination of log-linear density dependence and rainfall (with a 2-ye ...200616779559
[studies on the parasites of korean amphibia: 1. trematodes of amphibians]an investigation on the trematodes of korean amphibians was carried out during the period from april to september of 1976. a total of 883 amphibians of 6 species were examined. these frogs, toads and salamanders were collected in the areas of the han river side. and also these amphibians comprised 775 rana nigromaculata, 53 rana rugosa, 21 hyla arborea japonica, 2 bufo bufo gargarizans, 2 gallula tornieri and 30 hynobius leechi. out of the results of this work, 7 species of trematodes belonging ...197612913435
mitochondria-rich cells in anuran amphibia: chloride conductance and regional distribution over the body surface.the distribution and density (d(mrc)) of mitochondria-rich cells (mr cells) in skin epithelium, were determined over the whole body surface in nine species of anuran amphibia that live in a variety of habitats. it was found that the more terrestrial species (beginning with hyla arborea) have a higher density of mr cells in their pelvic region. in the skin of aquatic (xenopus laevis) or fossorial (pelobates syriacus) species, d(mrc) is evenly distributed over the whole body surface. in dorsal ski ...200010779739
metallothionein present or induced in the three species of frogs bombina orientalis, bufo bufo japonicus and hyla arborea japonica.a low molecular weight copper (cu)- and/or zinc (zn)-binding protein was present in the livers (and kidneys) of the three species of frogs bombina orientalis, bufo bufo japonicus and hyla arborea japonica. cadmium (cd) accumulated in the livers (and kidneys) after repeated i.m. injections of cd2+ was mostly bound to the same metal-binding protein as cd, zn- or cd, zn, cu-thionein. concentrations of cd and several essential elements in the livers and kidneys of the three species of frogs were det ...19846151449
accumulation of pigment granules around nuclei in early embryos of anura (amphibia).a dark spot was found to appear in each blastomere of the vegetal surface of blastulae of rana rugosa, hyla arborea. the spot divided into two before division of the blastomere, so that one new spot was allotted to each daughter cell. these dark spots were formed at early blastula stage, and persisted until the end of yolk plug stage. cytological observations showed that each dark spot corresponds to a mass of accumulated pigment granules around the nucleus of a blastomere. the accumulation incr ...19854078534
identification by immunofluorescence of prolactin- and somatotrophin-producing cells in the pituitary gland of the tree frog hyla arborea.the indirect immunofluorescence procedure was used to localize prolactin (prl)- and somatotrophin (sth)- producing cells in the pituitary distal lobe from hyla arborea adult specimens. the following mammalian antisera were employed: rabbit anti-ovine prl, antibovine prl, anti-human prl, anti-rat prl, anti-ovine sth, anti-bovine sth, anti-human sth; monkey anti-rat sth. immunocytochemical staining was suppressed by solid phase absorption of both anti-prl and anti-sth with the specific antigen. ab ...19853882518
gonadal sex differentiation in frogs: how testes become shorter than ovaries.testis differentiation in anuran amphibians is the result of two opposing processes: degeneration of the distal part, and development of the proximal part, which becomes a functional male gonad. undifferentiated gonad differentiates directly into a testis without a transition phase. we described the morphology of developing testes in rana temporaria and hyla arborea, and made careful histology and ultrastructure in pelophylax lessonae. the developing testis was divided into 10 stages (i-iii, und ...201323387847
within- and among-population impact of genetic erosion on adult fitness-related traits in the european tree frog hyla arborea.assessing in wild populations how fitness is impacted by inbreeding and genetic drift is a major goal for conservation biology. an approach to measure the detrimental effects of inbreeding on fitness is to estimate correlations between molecular variation and phenotypic performances within and among populations. our study investigated the effect of individual multilocus heterozygosity on body size, body condition and reproductive investment of males (that is, chorus attendance) and females (that ...201323250010
[phylogenetic studies on some japanese amphibia by mean of immunoelectrophoresis. i. relationships of some frogs and newt to toad (author's transl)].the phylogenetic relationship among toad, frogs and newt from japan was investigated by means of immunoelectrophoresis. homologous, heterologous and non-identity reactions between rabbit anti-bufo bufo antiserum and antigen from each of 13 species and 2 subspecies of japanese amphibia were examined. the number and positions of arcs appeared in these reactions were compared. the relationship observed in the comparison well coincided with the classification of the upper taxa of amphibia; viz 1) hy ...1975808417
ultrastructural changes in the dermal chromatophore unit of hyla arborea during color change.the structural changes in the chromatophores of hyla arborea related to changes in skin color were studied by electron microscopy and reflectance microspectrophotometry. during a change form a light to a darker green color, the melanosomes of the melanophores disperse and finally surround the iridophores and partly the xanthophores. the iridophores change from cup-shape to a cylindrical or conical shape with a simultaneous change in the orientation of the platelets from being parallel to the upp ...1978728971
the effect of stress and adrenaline on the color of hyla cinerea and hyla arborea. 1978571381
light and scanning microscopy of the tongue and its gustatory organs in the common toad, bufo bufo (l.).unlike frogs and european tree frogs, the common toad bufo bufo possesses a tongue lacking filiform papillae on its dorsal surface. instead, the mucosal epithelium forms irregular, high folds, dividing the surface of the tongue into numerous separate compartments. at the bottom of those compartments occur openings of tubular glands and singular ciliated cells. in a strongly distended tongue the folds of mucosa disappear, and the regions of glandular orifices assume a shape of pocket-like recesse ...1979117649
[joint development in xenoplastic transplants between anuran and urodele amphibians; correlations between shoulder joint and girdle; incompatibility in transplants of triton cristatus in hyla arborea and on discoglossus pictus]. 195613341315
warm vegetarians? heat waves and diet shifts in tadpoles.temperature can play an important role in determining the feeding preferences of ectotherms. in light of the warmer temperatures arising with the current climatic changes, omnivorous ectotherms may perform diet shifts toward higher herbivory to optimize energetic intake. such diet shifts may also occur during heat waves, which are projected to become more frequent, intense, and longer lasting in the future. here, we investigated how heat waves of different duration affect feeding preferences in ...201627870032
meteorological variables affecting onset and duration of calling in hyla arborea l. and bufo calamita calamita laur.it was the aim of this work to investigate the effects of meteorological factors on two types of behavior related to vocalization in two species of anuran amphibians (bufo calamita and hyla arborea). the measurements were carried out in two gravel pits in the kanton of zurich. evaluation by multiple linear regression was carried out at the computer center of zurich university. it could be shown that water temperature at 1900 was the most important factor for the onset of callings in both species ...197228313124
genetic bottlenecks driven by population disconnection.connectivity among populations plays a crucial role in maintaining genetic variation at a local scale, especially in small populations affected strongly by genetic drift. the negative consequences of population disconnection on allelic richness and gene diversity (heterozygosity) are well recognized and empirically established. it is not well recognized, however, that a sudden drop in local effective population size induced by such disconnection produces a temporary disequilibrium in allelic fre ...201020666803
novel natural peptides from hyla arborea schelkownikowi skin secretion.hyla arborea schelkownikowi is one of the leaf frog species inhabiting the southern territories of russia and the former ussr. this frog species is a member of the hylidae rafinesque, 1815 batrachians family. the present study deals with the previously uninvestigated peptidome of the hyla arborea schelkownikowi skin secretion. nano-electrospray ionization fourier transform mass spectrometry (nanoesi-ftms) of the skin secretion, in the intact form and after acetylation, was selected as the genera ...201020499319
immune challenges and visual signalling in tree frogs.in animals, mate-choice is often based on sexual signals that carry information and help the receiver make the best choice to improve the receiver's fitness. orange visual sexual signals have been hypothesised to carry immune information because they are often due to carotenoid pigments which are also involved in immunity response. although many studies have focused on the direct relationships between coloration and immunocompetence, few studies have simultaneously studied immunocompetent respon ...201728271177
does testosterone affect foraging behavior in male frogs?during the breeding season, males often produce costly and extravagant displays or physical ornaments to attract females. numerous studies have established that testosterone could directly influence the expression of certain sexual signals. however, few of these studies have focused on the indirect role that testosterone could play in modulating prey detection and visual performance to improve the foraging ability of males and hence their acquisition of nutritional resource. in the present study ...201728189643
trans-species variation in dmrt1 is associated with sex determination in four european tree-frog species.empirical studies on the relative roles of occasional xy recombination versus sex-chromosome turnover in preventing sex-chromosome differentiation may shed light on the evolutionary forces acting on sex-determination systems. signatures of xy recombination are difficult to distinguish from those of homologous transitions (i.e., transitions in sex-determination systems that keep sex-chromosome identity): both models predict x and y alleles at sex-linked genes to cluster by species. however, the x ...201626920488
differential effects of testosterone and 17β-estradiol on gonadal development in five anuran species.sex hormones are essential for sexual differentiation and play a key role in the development of gonads in amphibians. the goal of this study was to evaluate the influence of exogenous sex steroids, testosterone, and 17β-estradiol (e(2)) on development of gonads in five anuran species differing in their evolutionary positions, sex determination, and mode of gonadogenesis. we found that in two closely related species of fire-bellied toad, bombina bombina and bombina variegata, testosterone and e(2 ...201222641770
the economic efficiency of conservation measures for amphibians in organic farming--results from bio-economic modelling.this paper presents a whole farm bio-economic modelling approach for the assessment and optimisation of amphibian conservation conditions applied at the example of a large scale organic farm in north-eastern germany. the assessment focuses mainly on the habitat quality as affected by conservation measures such as through specific adapted crop production activities (cpa) and in-field buffer strips for the european tree frog (hyla arborea), considering also interrelations with other amphibian spec ...201323164542
empirical evidence for large x-effects in animals with undifferentiated sex chromosomes.reproductive isolation is crucial for the process of speciation to progress. sex chromosomes have been assigned a key role in driving reproductive isolation but empirical evidence from natural population processes has been restricted to organisms with degenerated sex chromosomes such as mammals and birds. here we report restricted introgression at sex-linked compared to autosomal markers in a hybrid zone between two incipient species of european tree frog, hyla arborea and h. orientalis, whose h ...201626868373
opportunity for sexual selection and effective population size in the lek-breeding european treefrog (hyla arborea).sexual selection in lek-breeding species might drastically lower male effective population size, with potentially important consequences for evolutionary and conservation biology. using field-monitoring and parental-assignment methods, we analyzed sex-specific variances in breeding success in a population of european treefrogs, to (1) help understanding the dynamics of genetic variance at sex-specific loci, and (2) better quantify the risk posed by genetic drift in this species locally endangere ...200919087183
mitochondrial and nuclear phylogeny of circum-mediterranean tree frogs from the hyla arborea group. 200818814852
long recording sequences: how to track the intra-individual variability of acoustic signals.recently developed acoustic technologies - like automatic recording units - allow the recording of long sequences in natural environments. these devices are used for biodiversity survey but they could also help researchers to estimate global signal variability at various (individual, population, species) scales. while sexually-selected signals are expected to show a low intra-individual variability at relatively short time scale, this variability has never been estimated so far. yet, measuring s ...201525970183
a key transcription cofactor on the nascent sex chromosomes of european tree frogs (hyla arborea).we show that med15, a key component of the transcription complex mediator, lies within the nonrecombining segment of nascent sex chromosomes in the male-heterogametic hyla arborea. both x and y alleles are expressed during embryonic development and differ by three frame-preserving indels (eight amino acids in total) within their glutamine-rich central part. these changes have the potential to affect the conformation of the mediator complex and to activate genes in a sex-specific way and might th ...200818622030
recombination is suppressed and variability reduced in a nascent y chromosome.several hypotheses have been elaborated to account for the evolutionary decay commonly observed in full-fledged y chromosomes. enhanced drift, background selection and selective sweeps, which are expected to result from reduced recombination, may all share responsibilities in the initial decay of proto-y chromosomes, but little empirical information has been gathered so far. here we take advantage of three markers that amplify on both of the morphologically undifferentiated sex chromosomes of th ...200717465927
prolactin, an activator of epithelial na+ channel, inhibits basolateral k+ channels in adult tree frog skin.adult amphibian skin actively transports na+ from its apical to basolateral side while in turn, k+ is recycled through na+, k+-atpase and k+ channels located in the basolateral membrane. we previously found that prl stimulates na+ transport in the skin of the adult tree frog (hyla arborea japonica) via an increase in the open-channel density of the epithelial na+ channel (enac). if prl also activates basolateral k+ channels, this activation would help to stimulate na+ transport, too. whether prl ...200515684589
sex-chromosome homomorphy in palearctic tree frogs results from both turnovers and x-y recombination.contrasting with birds and mammals, poikilothermic vertebrates often have homomorphic sex chromosomes, possibly resulting from high rates of sex-chromosome turnovers and/or occasional x-y recombination. strong support for the latter mechanism was provided by four species of european tree frogs, which inherited from a common ancestor (∼ 5 ma) the same pair of homomorphic sex chromosomes (linkage group 1, lg1), harboring the candidate sex-determining gene dmrt1. here, we test sex linkage of lg1 ac ...201525957317
tadpoles' responses to risk of fish introduction.the introduction of predatory species, such as fish, in amphibian breeding sites is one of the many likely causes of amphibian population decline. the existence of inducible or constitutive (permanent) defences is expected to temper the lethal effects of fish on tadpoles. according to current theories on the evolution of phenotypic plasticity, the amphibian species that occur in habitats that are mostly or often fish-free were expected to display inducible defences, while the species that reprod ...200312647168
speciation history and widespread introgression in the european short-call tree frogs (hyla arborea sensu lato, h. intermedia and h. sarda).european tree frogs (hyla) characterized by short temporal parameters of the advertisement call form six genetically differentiated but morphologically cryptic taxa, h. arborea sensu stricto, h. orientalis and h. molleri from across europe to western asia (together referred to as h. arborea sensu lato), two putative taxa within h. intermedia (northern and southern) from the italian peninsula and sicily, and h. sarda from sardinia and corsica. here, we assess species limits and phylogenetic relat ...201525482363
first-generation linkage map for the european tree frog (hyla arborea) with utility in congeneric species.western palearctic tree frogs (hyla arborea group) represent a strong potential for evolutionary and conservation genetic research, so far underexploited due to limited molecular resources. new microsatellite markers have recently been developed for hyla arborea, with high cross-species utility across the entire circum-mediterranean radiation. here we conduct sibship analyses to map available markers for use in future population genetic applications.201425430653
sex-chromosome differentiation parallels postglacial range expansion in european tree frogs (hyla arborea).occasional xy recombination is a proposed explanation for the sex-chromosome homomorphy in european tree frogs. numerous laboratory crosses, however, failed to detect any event of male recombination, and a detailed survey of nw-european hyla arborea populations identified male-specific alleles at sex-linked loci, pointing to the absence of xy recombination in their recent history. here, we address this paradox in a phylogeographic framework by genotyping sex-linked microsatellite markers in popu ...201425209463
range-wide sex-chromosome sequence similarity supports occasional xy recombination in european tree frogs (hyla arborea).in contrast with mammals and birds, most poikilothermic vertebrates feature structurally undifferentiated sex chromosomes, which may result either from frequent turnovers, or from occasional events of xy recombination. the latter mechanism was recently suggested to be responsible for sex-chromosome homomorphy in european tree frogs (hyla arborea). however, no single case of male recombination has been identified in large-scale laboratory crosses, and populations from nw europe consistently displ ...201424892652
stronger transferability but lower variability in transcriptomic- than in anonymous microsatellites: evidence from hylid frogs.a simple way to quickly optimize microsatellites in nonmodel organisms is to reuse loci available in closely related taxa; however, this approach can be limited by the stochastic and low cross-amplification success experienced in some groups (e.g. amphibians). an efficient alternative is to develop loci from transcriptome sequences. transcriptomic microsatellites have been found to vary in their levels of cross-species amplification and variability, but this has to date never been tested in amph ...201424345298
conservation phylogeography: does historical diversity contribute to regional vulnerability in european tree frogs (hyla arborea)?documenting and preserving the genetic diversity of populations, which conditions their long-term survival, have become a major issue in conservation biology. the loss of diversity often documented in declining populations is usually assumed to result from human disturbances; however, historical biogeographic events, otherwise known to strongly impact diversity, are rarely considered in this context. we apply a multilocus phylogeographic study to investigate the late-quaternary history of a tree ...201324102652
homologous sex chromosomes in three deeply divergent anuran species.comparative genomic studies are revealing that, in sharp contrast with the strong stability found in birds and mammals, sex determination mechanisms are surprisingly labile in cold-blooded vertebrates, with frequent transitions between different pairs of sex chromosomes. it was recently suggested that, in context of this high turnover, some chromosome pairs might be more likely than others to be co-opted as sex chromosomes. empirical support, however, is still very limited. here we show that sex ...201323888863
maintenance of ancestral sex chromosomes in palearctic tree frogs: direct evidence from hyla orientalis.contrasting with the situation found in birds and mammals, sex chromosomes are generally homomorphic in poikilothermic vertebrates. this homomorphy was recently shown to result from occasional x-y recombinations (not from turnovers) in several european species of tree frogs (hyla arborea, h. intermedia and h. molleri). because of recombination, however, alleles at sex-linked loci were rarely diagnostic at the population level; support for sex linkage had to rely on multilocus associations, combi ...201323735903
impact assessment of a high-speed railway line on species distribution: application to the european tree frog (hyla arborea) in franche-comté.the aim of the present work is to assess the potential long-distance effect of a high-speed railway line on the distribution of the european tree frog (hyla arborea) in eastern france by combining graph-based analysis and species distribution models. this combination is a way to integrate patch-level connectivity metrics on different scales into a predictive model. the approach used is put in place before the construction of the infrastructure and allows areas potentially affected by isolation t ...201323685273
fine structural changes in the endolymphatic sac induced by calcium loading in the tree frog, hyla arborea japonica.the growth rate of calcium carbonate (caco3) crystals in the endolymphatic sac was modulated, and morphological changes in this organ were observed by light and electron microscopy. when calcium chloride (cacl2) was given to the three frog for a short period (3 days to 2 weeks), caco3 crystal production was accelerated. epithelial cells enlarged, their rough endoplasmic reticulum (rer) and golgi apparatus developed, and dense material increased around caco3 crystals and/or in the endolymphatic l ...19902268475
cryptic recombination in the ever-young sex chromosomes of hylid frogs.sex chromosomes are expected to evolve suppressed recombination, which leads to degeneration of the y and heteromorphism between the x and y. some sex chromosomes remain homomorphic, however, and the factors that prevent degeneration of the y in these cases are not well understood. the homomorphic sex chromosomes of the european tree frogs (hyla spp.) present an interesting paradox. recombination in males has never been observed in crossing experiments, but molecular data are suggestive of occas ...201222901240
cryptic diversity among western palearctic tree frogs: postglacial range expansion, range limits, and secondary contacts of three european tree frog lineages (hyla arborea group).we characterize divergence times, intraspecific diversity and distributions for recently recognized lineages within the hyla arborea species group, based on mitochondrial and nuclear sequences from 160 localities spanning its whole distribution. lineages of h. arborea, h. orientalis, h. molleri have at least pliocene age, supporting species level divergence. the genetically uniform iberian h. molleri, although largely isolated by the pyrenees, is parapatric to h. arborea, with evidence for succe ...201222652054
heterozygosity-fitness correlations among wild populations of european tree frog (hyla arborea) detect fixation load.quantifying the impacts of inbreeding and genetic drift on fitness traits in fragmented populations is becoming a major goal in conservation biology. such impacts occur at different levels and involve different sets of loci. genetic drift randomly fixes slightly deleterious alleles leading to different fixation load among populations. by contrast, inbreeding depression arises from highly deleterious alleles in segregation within a population and creates variation among individuals. a popular app ...201121410805
consequences of genetic erosion on fitness and phenotypic plasticity in european tree frog populations (hyla arborea).the detrimental effects of genetic erosion on small isolated populations are widely recognized contrary to their interactions with environmental changes. the ability of genotypes to plastically respond to variability is probably essential for the persistence of these populations. genetic erosion impact may be exacerbated if inbreeding affects plastic responses or if their maintenance were at higher phenotypic costs. to understand the interplay 'genetic erosion-fitness-phenotypic plasticity', we ...201120964778
hearing is not necessarily believing in nocturnal anurans.the recent discovery of the use of visual cues for mate choice by nocturnal acoustic species raises the important, and to date unaddressed, question of how these signals affect the outcome of mate choice predicted by female preference for male calls. in order to address this question, we presented female hyla arborea tree frogs with a series of choices between combinations of acoustic and visual cues of varying quality in nocturnal conditions. while females exhibited the expected preference for ...201020335196
growth aspects of calcium carbonate crystals of the endolymphatic sac in the tree frog (hyla arborea japonica): a pulse-chase study. 19873449594
[a fine structure and carbohydrate histochemistry of the lingual glands of the tree frog (hyla arborea japonica)]. 19863467261
lung of the tree frog, hyla arborea l. a scanning and transmission electron microscopic study.lungs of hyla arborea l. were examined by scanning and transmission electron microscopy and morphometric methods. the lungs contain several interconnected folds in a netlike reticular arrangement of first, second and third order, mainly covered with pneumocytes. on the septa of first and second order, irregularly distributed small patches of ciliated epithelium devoid of goblet cells are located. dome-shaped neuroepithelial bodies can be seen in the vicinity of ciliated epithelium. the pulmonary ...19863766994
limb regenerative capacity of four species of japanese frogs of the families hylidae and ranidae.the regenerative capacity of limbs was investigated by amputation of limbs at the zeugopodium in postmetamorphic froglets and adults of various sizes in four species of japanese frogs, all of which showed some regeneration at these ages. in hyla arborea japonica and rana brevipoda porosa most young froglets regenerated their limbs well; however, the rate of regeneration decreased with the age of amputation, and the limb became nonregenerative in adults. limbs of adults in rana rugosa and r. japo ...19827120419
[metamorphosis of hyla arborea and of rana esculenta in conditions of environmental pollution: a biostatistical study].an extention to the largest possible numbers of animal species is a basic requirement when damages induced by pollutants are studies with a specific purpose of defining the concentrations of pollutants compatible with the conservation of the biochemical system. animals should be bred in standard conditions, and the natural habitat is to be reproduced in the laboratory. the studies should also be planned favoring these statistical elaborations which are suitable of application also in case of int ...19816976178
neuroepithelial bodies in the lung of the tree frog, hyla arborea l. a scanning and transmission electron microscopic study.the endocrine-like cells (elc), which together with nerve endings form the neuroepithelial bodies, are located on primary and secondary septa in the non-ciliated epithelium of the lung of hyla arborea. elc protrude markedly toward the lumen of the lung and are surrounded by pneumocytes, which separate elc from the lumen by thin cytoplasmic processes. elc possess a light cytoplasm containing two types of granules: (i) numerous small granules, 50-110 nm in diameter, and (ii) single large granules, ...19817237539
multiple signals and male spacing affect female preference at cocktail parties in treefrogs.effective acoustic communication in the face of intense conspecific background noise constitutes a constant sensory challenge in chorusing and colonial species. an evolutionary approach suggests that behavioural and environmental constraints in these species should have shaped signal design and signalling behaviour to enable communication in noisy conditions. this could be attained both through the use of multicomponent signals and through short-term adjustments in the spatial separation of call ...201020018785
n-terminal tagging strategy for de novo sequencing of short peptides by esi-ms/ms and maldi-ms/ms.the major portion of skin secretory peptidome of the european tree frog hyla arborea consists of short peptides from tryptophyllin family. it is known that b-ions of these peptides undergo head-to-tail cyclization, forming a ring that can open, resulting in several linear forms. as a result, the spectrum contains multiple ion series, thus complicating de novo sequencing. this was observed in the q-tof spectrum of one of the tryptophyllins isolated from hyla arborea; the sequence flpffp-nh(2) was ...201019836262
structure of the mating calls and relationships of the european tree frogs (hylidae, anura).as an extension of earlier investigations, the mating calls ofhyla arborea kretensis, hyla a. molleri, andhyla a. sarda were studied, and variations in the calls with air temperature were determined. the mating calls of these species exhibit the typical structure, made up of short pulses of varying amplitude. the call duration and the inter-call interval decrease linearly with increasing temperature (over the range in which calls can occur), whereas the number of pulses per call does not change. ...197428308100
[the fine structure of the laryngeal muscles of the tree frog, hyla arborea arborea (l)., compared with that of a skeletal muscle (author's transl)]. 19734743255
[vitellogenesis of mitochondrial origin in the ovocyte of hyla arborea l. (anourous amphibian)]. 19724640397
in vitro assay of msh using skin from the frog hyla arborea. 19704322663
[perfusion fixation of frog pancreas using various solutions (hyla arborea, rana esculenta, rana temporaria)]. 19704941793
[studies on the development of xenoplastic eye chimerae in amphibians].1. in order to evaluate the development of the xenoplastic eye chimerae, stages of differentiation of the normal eye inbombina variegata, hyla arborea andtriturus alpestris between the second and ninth day after neurulation have been worked out, and the results are summarized in table 1. 2. four types of chimeric eyes developed after transplantation of the eye-cup anlagen: type i, species-specific donor eye cup with a host lens; type ii, host lens with a chimeric eye cup which was made of donor ...196928304449
[rhythm of acoustic activity of hyla arborea (linné) and hyla meridionalis boettger anourous amphibians]. 19674229306
[cytomorphosis of mauthner's sheath in the hyla arborea savignyi; in normal development and in experimentally induced metamorphosis]. 201514921650
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