| development of natural products as drugs acting on central nervous system. | we have recently studied several natural product constituents which have effects on the cns. (1) tetrahydropalmatine (thp) and its analogues were isolated from corydalis ambigua and various species of stephania. (+)-thp and (-)-thp possess not only analgesic activity, but also exert sedative-tranquilizing and hypnotic actions. results of receptor binding assay and their pre- and post-synaptic effects on dopaminergic system indicate that (-)-thp and (-)-stepholidine are dopamine receptor antagoni ... | 1991 | 1841995 |
| isolation, chemical and biological characterization of margaratensin, a neurotensin-related peptide from the skin of rana margaratae. | a new neurotensin (nt)-related peptide, margaratensin, was obtained by sep-pak c18 and rp-hplc from methanol extracts of the skin of chinese frog rana margaratae. the structure of the peptide has been determined to be asp-lys-arg-pro-tyr-ile-leu-his-glu, which is found to be homologous to the cooh-terminal sequence of nt, but has an extra his-glu at the cooh-terminus. the synthetic preparation was shown to be indistinguishable from the native peptide during hplc, amino acid analysis and bioassay ... | 1990 | 2282148 |
| amphibian tachykinin precursor. | the precursor of amphibian tachykinin has not been found although more than 30 tachykinins have been isolated from amphibians since 1964. in this report, two tachykinin-like peptides are identified from the skin secretions of the frog, odorrana grahami. their amino acid sequences are ddtedlankfiglm-nh(2) (named tachykinin og1) and ddasdrakkfyglm-nh(2) that is the same with ranamargarin found in rana margaretae, respectively, with a conserved fxglm-nh(2) c-terminal consensus motif. by cdna clonin ... | 2006 | 17045570 |
| lineage divergence in odorrana graminea complex (anura: ranidae: odorrana). | the confusing and unstable taxonomy of odorrana livida (rana livida) since its first record has made it a focal frog complex for systematics. in china, four species, odorrana nebulosa, o. graminea, o. sinica, o. leporipes, were described to closely resemble o. livida or o. chloronota based on their morphological similarities, accompanied by much taxonomic confusion because of ambiguities in the wide distribution and morphological variations. currently o. graminea is being used as the name of a p ... | 2015 | 26249398 |
| om-lv20, a novel peptide from odorous frog skin, accelerates wound healing in vitro and in vivo. | the healing of chronic wounds remains a considerable challenge in clinical trials and imposes severe financial and physiological burdens on patients. many works are being tried to find ideal clinical promoting wound healing biomaterials. small bioactive peptides with low cost and easy production, store and transfer become excellent candidates. here, we identified a novel peptide (named om-lv20) from skin secretions of odorous frog odorrana margaretae. the peptide had an amino acid sequence of "l ... | 0 | 28650592 |
| complete mitochondrial genome of the green odorous frog odorrana margaretae (anura: ranidae). | the complete mitochondrial genome (mitogenome) of the green odorous frog odorrana margaretae (anura: ranidae) has been studied. the 17,903 bp circular genome contains the typical complement of 13 protein-coding genes, 2 ribosomal rnas, 22 transfer rnas, and a control region. the at content of the overall base compositon of h-strand is 56% and the length of control region is 2501 bp with 63.8% at content. the arrangement of the protein-coding and ribosomal rna genes was the same as that found in ... | 2015 | 24938085 |
| transcriptome profile of the green odorous frog (odorrana margaretae). | transcriptome profiles provide a practical and inexpensive alternative to explore genomic data in non-model organisms, particularly in amphibians where the genomes are very large and complex. the odorous frog odorranamargaretae (anura: ranidae) is a dominant species in the mountain stream ecosystem of western china. limited knowledge of its genetic background has hindered research on this species, despite its importance in the ecosystem and as biological resources. here we report the transcripto ... | 2013 | 24073255 |
| sulpiride attenuates ranatensin-m-induced antinociception. | intracerebroventricular (icv) administration of ranatensin-m (rm), a bombesin-like peptide isolated from the skin of chinese frog rana margaratae, produced a dose-dependent prolongation in the hot-plate latency in mice. naloxone 1, 2, or 10 mg.kg-1 ip failed to antagonize the effects of rm. however, rm-induced antinociception was attenuated by pretreatment with sulpiride (sul, 100 mg.kg-1, ip), a selective da2 receptor blocker. sul (100 mg.kg-1, ip) did not affect hot-plate latencies when admini ... | 1991 | 1807073 |
| isolation and structure of ranamargarin, a new tachykinin from the skin of chinese frog rana margaratae. | a new tetradecapeptide, ranamargarin, has been isolated by sep-pak c18 and hplc from methanol extracts of the skin of the chinese frog rana margaratae. the sequence of the peptide is: asp-asp-ala-ser-asp-arg-ala-lys-lys-phe-tyr-gly-leu-met-nh2. this structure has been confirmed by synthesis. the peptide is the largest among the amphibian tachykinins and its n-terminal amino acids are quite different from those of the other tachykinins. the formation of the sulfoxide and peak-splitting of ranamar ... | 1989 | 2803524 |
| identification and characterization of a novel gene-encoded antioxidant peptide obtained from amphibian skin secretions. | amphibian skin is known to secrete gene-encoded antioxidant peptides of small molecular weight, which play important roles in host defense. however, recognition of such peptides is still in its infancy. here, we discovered a novel gene-encoded antioxidant peptide (named om-gf17) from skin secretions of amphibian species, odorrana margaretae. produced by the post-translational processing of a 61-residue prepropeptide, the amino acid sequence of om-gf17 was 'gffkwhprcgeehsmwt', with a molecular ma ... | 2020 | 30470149 |
| evolutionary melting pots and reproductive isolation: a ring-shaped diversification of an odorous frog (odorrana margaratea) around the sichuan basin. | quaternary climatic oscillations and geography are of primary importance in shaping intraspecific genetic diversity. we examined the diversification patterns and inferred processes for the green odorous frog (odorrana margaretae) of western china. species distribution modelling showed that the species has a continuous circular distribution around the sichuan basin while the basin itself is largely uninhabitable. population genetic and phylogenetic analyses revealed that the species has a ring-sh ... | 2018 | 30325564 |