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characterization of l-asparagine transport systems in stemphylium botryosum.l-asparagine uptake by stemphylium botryosum is mediated by two distinct energy- and temperature-dependent transport systems. one permease is relatively specific for l-asparagine and l-glutamine and is present in nutrient-sufficient mycelium. the specific permease shows an optimum ph at 5.2, saturation kinetics (km = 4.4 x 10(-4) m, vmax = 1.1 mumol/g per min), competitive gradient of l-asparagine, and higher affinity towards the l-isomer of asparagine. amide derivatives of l-asparagine (5-diazo ...19768427
senescence and death of the conidiogenous cell in stemphylium botryosum wallroth. 19734360416
a fine-structural study of conidium initiation in stemphylium botryosum wallroth. 19724561137
surface structure of the conidium and conidiophore of stemphylium botryosum. 19734572484
comparative abilities of stemphylium botryosum and helminthosporium turcicum to induce and degrade a phytoalexin from alfalfa. 19695392960
degradation of alfalfa phytoalexin by stemphylium botryosum. 19695392961
[the effect of common leaf spot (pseudopeziza medicaginis (lib.) sacc.) and leaf spot (stemphylium botryosum wallr.; phoma medicaginis malbr. et roum.) on the co2 assimilation by the leaves of alfalfa (medicago sativa l.)]. 19695396112
[stability of pigmented fungi stemphylium botryosum wallr and cladosporium cladosporivides (freis) de vries towards gamma-irradiation]. 19685753849
a novel enolic beta-ketoaldehyde phytotoxin produced by stemphylium botryosum f. sp. lycopersici : partial chemical and biological characterization.a new phytotoxin, stemphyloxin i, c(21)h(32)o(5), was isolated from cultures of the pathogenic fungus stemphylium botryosum f. sp. lycopersici. the toxin is a tricyclic compound possessing a most unusual beta-ketoaldehyde group. injection of stemphyloxin i into a tomato leaflet caused unlimited necrotic spots and a loss of turgor, which at higher toxin concentration wilted the whole compound leaf. visible symptoms could be observed at a toxin concentration as low as 2.7 micromolar. stemphyloxin ...198216662165
crystallization and x-ray analysis of stemphyloxin i, a phytotoxin from stemphylium botryosum.certain isolates of the plant-pathogenic fungus stemphylium botryosum produce a phytotoxin, stemphyloxin i. this toxin (c(21)h(34)o(6)) was crystallized and its structure was determined by x-ray crystallography to be a beta-ketoaldehyde trans-decalin. this compound is a highly unusual natural product. iron (fe(3+)) controls production of toxin by this fungus. furthermore, iron reacts with the toxin to yield a colored product which aids in its detection on chromatograms and in its quantitative es ...198317754554
mutagenicity of stemphyltoxin iii, a metabolite of alternaria alternata.some common decay organisms of vegetables and ripened fruits are alternaria species. even fruits and vegetables kept under refrigeration can be spoiled by alternaria species because the mold grows at low temperatures. alternaria alternata is commonly found in grain in areas with a high incidence of esophageal cancer. three metabolites, altertoxins i, ii, and iii, have been isolated from a. alternata and have hydroxyperylenequinone structures. although other perylenequinone metabolites such as st ...19912036005
identification of allergens homologous to alt a 1 from stemphylium botryosum and ulocladium botrytis.several studies have demonstrated that proteins homologous to the alt a 1 major allergen of alternaria alternata are expressed in other members of the pleosporaceae family. however, since no direct biochemical data have been reported concerning the presence of alt a 1 allergen homologues capable of binding ige in the excretion-secretion products of stemphylium and ulocladium, our objective was to explore their presence in stemphylium botryosum and ulocladium botrytis. s. botryosum and u. botryti ...201121504377
phylogeny of stemphylium spp. based on its and glyceraldehyde-3-phosphate dehydrogenase gene sequences.the phylogenetic relationships among 44 isolates representing 16 species of stemphylium were inferred from its and glyceraldehyde-3-phosphate dehydrogenase (gpd) sequence data. the results generally agree with morphological species concepts. there was strong support for monophyly of the genus stemphylium. analysis of the gpd fragment in particular was useful for establishing well-supported relationships among the species and isolates of stemphylium. species of stemphylium that appear to have los ...200221156539
isolation and characterization of stemphylin, a chromone glucoside from stemphylium botryosum.a phytotoxic compound was isolated from a liquid culture medium of stemphylium botryosum, a pathogen of lettuce. the toxin is an amorphous yellow solid with absorbance maxima at 218, 268, and 427.5 nm and exhibits a bathochromic shift in alkaline ph. it has a molecular weight of 370 and an empirical formula of c(17)h(22)o(9). glucose and aromatic pigments are detected after acid hydrolysis. based on its spectral and chemical properties, the proposed structure of the toxin is 3-hydroxy- 2,2-dimet ...197516659141
restriction mapping of the igs region in alternaria spp. reveals variable and conserved domains.accurate identification of alternaria spp. is dependent upon the production of diagnostic morphological characters under defined cultural conditions and the proper assessment of character variation. this process is often compromised by variation in laboratory facilities and technical expertise. to assist taxon identification and phylogenetic studies, restriction site information from the intergenic spacer (igs) region of nuclear rdna was evaluated. restriction maps were constructed from 15 speci ...200515736866
allergen detection from 11 fungal species before and after germination.allergens dispersed by airborne fungal spores play an important but poorly understood role in the underlying cause and exacerbation of asthma. previous studies suggest that spores of alternaria and aspergillus release greater quantities of allergen after germination than before germination. it is unknown whether this is true of other allergenic fungi.200312589346
production of a toxin stemphol by stemphylium species.five of 11 isolates of stemphylium botryosum wallr. from oilseed rape produced the phytotoxin stemphol when cultured on rice, with yields varying from 15.0 to 98.4 mg/kg, and three of them also produced the toxin on czapek dox (cd) liquid medium supplemented with cations (0.12-0.31 mg/l) and potato-dextrose (pd) broth (0.37-1.47 mg/l). in liquid cultures about 95% of stemphol was found in the mycelium, and toxin production was slightly increased when cultures were exposed to near uv light rather ...19948032689
stemphyltoxin iii from alternaria alternata.stemphyltoxin iii, (6ar*, 6bs*, 7r*, 8r*)-3,6a, 10-trihydroxy-4,9-dioxo-4,6a,6b,7,8,9-hexahydro-7,8-epoxyperylene, a known metabolite of stemphylium botryosum var. lactucum, has been identified as a mutagenic metabolite of alternaria alternata by spectroscopic studies. the 13c-nmr spectral data, which were not reported previously, are presented.19892746264
the major allergen of alternaria alternata (alt a 1) is expressed in other members of the pleosporaceae family.there is general consensus regarding the scarce cross-reactivity existing between alternaria alternata and other allergenic moulds such as aspergillus fumigatus, penicillium notatum or cladosporium herbarum. however, a. alternata has been shown to have a very significant level of allergenic cross-reactivity with other fungi belonging to the pleosporaceae family. to date, no biological identity or homologies with other proteins have been described for alt a 1, and it remains unclear whether the m ...200616466440
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