| microsatellites mapping for non-model species with chromosomal rearrangement: a case study in the frog quasipaa boulengeri (anura: dicroglossidae). | gene mapping is an important resource for understanding the evolution of genes and cytogenetics. model species with a known genetic map or genome sequence allow for the selection of genetic markers on a desired chromosome, while it is hard to locate these markers on chromosomes of non-model species without such references. a frog species, quasipaa boulengeri, shows chromosomal rearrangement polymorphisms, making itself a fascinating model for chromosomal speciation mediated by suppressed recombi ... | 2017 | 28727488 |
| next-generation sequencing of mixed genomic dna allows efficient assembly of rearranged mitochondrial genomes in amolops chunganensis and quasipaa boulengeri. | recent improvements in next-generation sequencing (ngs) technologies can facilitate the obtainment of mitochondrial genomes. however, it is not clear whether ngs could be effectively used to reconstruct the mitogenome with high gene rearrangement. these high rearrangements would cause amplification failure, and/or assembly and alignment errors. here, we choose two frogs with rearranged gene order, amolops chunganensis and quasipaa boulengeri, to test whether gene rearrangements affect the mitoge ... | 2016 | 27994980 |
| intraspecific rearrangement of mitochondrial genome suggests the prevalence of the tandem duplication-random loss (tdlr) mechanism in quasipaa boulengeri. | tandem duplication followed by random loss (tdrl) is the most frequently invoked model to explain the diversity of gene rearrangements in metazoan mitogenomes. the initial stages of gene rearrangement are difficult to observe in nature, which limits our understanding of incipient duplication events and the subsequent process of random loss. intraspecific gene reorganizations may represent intermediate states, and if so they potentially shed light on the evolutionary dynamics of tdrl. | 2016 | 27881087 |
| the complete mitochondrial genome of quasipaa boulengeri (anura: dicroglossidae). | quasipaa boulengeri, an asian spiny frog that belongs to neobatrachia, has been reported as a de novo case of chromosomal rearrangements. in this paper, we determined the whole mitochondrial dna (mtdna) sequence of q. boulengeri. the complete mtdna sequence of q. boulengeri is 17,741 bp in length, which contains 13 protein-coding genes (pcgs), two rrnas, 23 trnas and one control region. the overall nucleotides base composition of the complete mtdna is a (28.86%), g (14.43%), c (26.09%), t (30.62 ... | 2014 | 23841602 |
| geological events play a larger role than pleistocene climatic fluctuations in driving the genetic structure of quasipaa boulengeri (anura: dicroglossidae). | paleoclimatic and paleogeological events have been identified as being the two main drivers of genetic structuring in extant organisms. we used a montane stream-dwelling frog, quasipaa boulengeri, to explore the relative roles played by these drivers on species in southern china, a region needing thorough studies. we detected four major matrilines, and no broadly distributed haplotypes occurred. the complex orogenesis of south-western china drove matrilineal divergence in q. boulengeri into high ... | 2013 | 23216961 |
| a de novo case of floating chromosomal polymorphisms by translocation in quasipaa boulengeri (anura, dicroglossidae). | very few natural polymorphisms involving interchromosomal reciprocal translocations are known in amphibians even in vertebrates. in this study, thirty three populations, including 471 individuals of the spiny frog quasipaa boulengeri, were karyotypically examined using giemsa stain or fish. five different karyomorphs were observed. the observed heteromorphism was autosomal but not sex-related, as the same heteromorphic chromosomes were found both in males and females. our results indicated that ... | 2012 | 23056254 |
| interspecies introgressive hybridization in spiny frogs quasipaa (family dicroglossidae) revealed by analyses on multiple mitochondrial and nuclear genes. | introgression may lead to discordant patterns of variation among loci and traits. for example, previous phylogeographic studies on the genus quasipaa detected signs of genetic introgression from genetically and morphologically divergent quasipaa shini or quasipaa spinosa. in this study, we used mitochondrial and nuclear dna sequence data to verify the widespread introgressive hybridization in the closely related species of the genus quasipaa, evaluate the level of genetic diversity, and reveal t ... | 2018 | 29375796 |
| the origin and evolution of chromosomal reciprocal translocation in quasipaa boulengeri (anura, dicroglossidae). | chromosomal rearrangements have long fascinated evolutionary biologists for being widely implicated in causing genetic differentiation. suppressed recombination has been demonstrated in various species with inversion; however, there is controversy over whether such recombination suppression would facilitate divergence in reciprocal translocation with reduced fitness. in this study, we used the spiny frog, quasipaa boulengeri, whose western sichuan basin populations exhibit translocation polymorp ... | 2019 | 32038718 |
| sex chromosomal dimorphisms narrated by x-chromosome translocation in a spiny frog (quasipaa boulengeri). | in the general model of sex chromosome evolution for diploid dioecious organisms, the y (or w) chromosome is derived, while the homogametic sex presumably represents the ancestral condition. however, in the frog species quasipaa boulengeri, heteromorphisms caused by a translocation between chromosomes 1 and 6 are not related to sex, because the same heteromorphic chromosomes are found both in males and females at the cytological level. to confirm whether those heteromorphisms are unrelated to se ... | 2018 | 30505335 |
| suppressed recombination of sex chromosomes is not caused by chromosomal reciprocal translocation in spiny frog (quasipaa boulengeri). | chromosome rearrangements (crs) are perceived to be related to sex chromosome evolution, but it is a matter of controversy whether crs are the initial causative mechanism of suppressed recombination for sex differentiation. the early stages of sex chromosome evolution in amphibians may represent intermediate states of differentiation, and if so, they potentially shed light on the ultimate cause of suppressed recombination and the role of crs in sex chromosome differentiation. in this paper, we s ... | 2018 | 30210524 |
| complete mitochondrial genomes of nanorana taihangnica and n. yunnanensis (anura: dicroglossidae) with novel gene arrangements and phylogenetic relationship of dicroglossidae. | complete mitochondrial (mt) genomes have been used extensively to test hypotheses about microevolution and to study population structure, phylogeography, and phylogenetic relationships of anura at various taxonomic levels. large-scale mt genomic reorganizations have been observed among many fork-tongued frogs (family dicroglossidae). the relationships among dicroglossidae and validation of the genus feirana are still problematic. hence, we sequenced the complete mt genomes of nanorana taihangnic ... | 2018 | 29486721 |