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subtercola boreus gen. nov., sp. nov. and subtercola frigoramans sp. nov., two new psychrophilic actinobacteria isolated from boreal groundwater.psychrophilic actinobacterial isolates from permanently cold groundwater in finland were characterized using a polyphasic approach. growth on agar plates was observed at temperatures down to -2 degrees c, with an optimum at 15-17 degrees c, but no growth was observed at 30 degrees c. the peptidoglycan type was b2y and the characteristic diamino acid was diaminobutyric acid. the cell wall sugars of strain k265t were rhamnose, ribose, xylose and mannose and those of strain k300t were glucose, rham ...200011034481
diversity of grass-associated microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of subtercola pratensis sp. nov., curtobacterium herbarum sp. nov. and plantibacter flavus gen. nov., sp. nov.a representative selection of coryneform bacteria, isolated from the phyllosphere of grasses and the litter layer after mulching the sward, was characterized by a polyphasic approach to clarify their taxonomic position in the family microbacteriaceae, with particular reference to potentially plant-pathogenic bacteria. on the basis of 16s rdna analysis, the isolates can be classified into six genotypes representing the genera curtobacterium, clavibacter, subtercola and a subgroup, which was not a ...200212361245
rhodoglobus vestalii gen. nov., sp. nov., a novel psychrophilic organism isolated from an antarctic dry valley lake.a novel, psychrophilic, gram-positive bacterium (designated strain lv3t) from a lake near the mcmurdo ice shelf, antarctica, has been isolated and characterized. this organism formed red-pigmented colonies, had an optimal growth temperature of 18 degrees c and grew on a variety of media between -2 and 21 degrees c. scanning electron micrographs of strain lv3t that showed small rods with unusual bulbous protuberances during all phases of growth were of particular interest. the g + c content of th ...200312892115
reclassification of subtercola pratensis behrendt et al. 2002 as agreia pratensis comb. nov.comparative analysis of 16s rdna sequences revealed a close phylogenetic relationship (99.6 % similarity) between subtercola pratensis behrendt et al. 2002 and agreia bicolorata evtushenko et al. 2001. the two species were found to share genus-specific chemotaxonomic characteristics such as the occurrence of d-ornithine and l-2,4-diaminobutyric acid in the peptidoglycan and the profile of cellular fatty acids and 1,1-dimethoxy-alkanes. dna-dna relatedness of only 47.8 % and differences in phenot ...200314657142
microcella putealis gen. nov., sp. nov., a gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater.three gram-positive bacteria designated cv-2t, cv-40 and ac-30 were isolated from a highly alkaline groundwater environment (ph 11.4). these organisms formed very small rod-shaped cells, are aerobic, non-spore-forming, catalase-positive, oxidase-negative, with an optimum growth temperature of 35 degrees c and optimum ph value of growth between 8.5 and 9.0. the strains possessed a novel b-type cell-wall peptidoglycan structure with lysine as the diamino acid; the major respiratory quinones were m ...200516104348
sugar beet-associated bacterial and fungal communities show a high indigenous antagonistic potential against plant pathogens.the aim of this study was to analyze microbial communities in/on sugar beet with special focus on antagonists toward plant pathogens. for this purpose, the composition of microorganisms isolated from the rhizosphere, phyllosphere, endorhiza, and endosphere of field-grown sugar beet plants was analyzed by a multiphasic approach at three different plant development stages at six locations in europe. the analysis of microbial communities by single strand conformation polymorphism (sscp) of 16s/18s ...200818060449
microterricola viridarii gen. nov., sp. nov., a new member of the family microbacteriaceae.strain kv-677t, a gram-positive, aerobic, motile, rod-shaped bacterium, was isolated from park soil in tokyo, japan, and characterized. it grew well at 15-30 degrees c on nutrient agar and colonies were pale yellow. the cell-wall peptidoglycan contained diaminobutyric acid, glycine, alanine and glutamic acid and the muramic acid acyl type was acetyl. the predominant menaquinone was mk-12. mycolic acids were not detected. the dna g+c content was 70 mol%. 16s rrna gene sequence analysis revealed t ...200818398212
glaciibacter superstes gen. nov., sp. nov., a novel member of the family microbacteriaceae isolated from a permafrost ice wedge.gram-positive, aerobic, non-spore-forming, irregular rod-shaped bacteria, designated strains ahu1791(t) and ahu1810, were isolated from a permafrost ice wedge in alaska. cells were motile by means of a polar flagellum. the strains were psychrophilic, growing at -5 to 25 degrees c. phylogenetic analysis of 16s rrna gene sequences indicated that the ice-wedge isolates formed a clade distinct from other genera affiliated with the family microbacteriaceae. the novel strains showed highest levels of ...200919244426
culturable bacterial diversity at the princess elisabeth station (utsteinen, sør rondane mountains, east antarctica) harbours many new taxa.we studied the culturable heterotrophic bacterial diversity present at the site of the new princess elisabeth station at utsteinen (dronning maud land, east antarctica) before construction. about 800 isolates were picked from two terrestrial microbial mat samples after incubation on several growth media at different temperatures. they were grouped using rep-pcr fingerprinting and partial 16s rrna gene sequencing. phylogenetic analysis of the complete 16s rrna gene sequences of 93 representatives ...201121501941
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