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[toxin-producing ability of molds of the genus alternaria].the ability to produce the alternaria mycotoxins alternariol (aoh), alternariol monomethyl ether (ame) and altenuene (alt) were studied on 87 alternaria strains. 50 isolates were studied to produce tenuazonic acid (tea). the strains were isolated from ccm-maize and stored hay. they were cultured under optimum conditions and analysed by various chromatographic methods for the 4 mycotoxins. all alternaria strains studied produced ame and tea, 77 per cent also aoh. a production of alt was detected ...19921609553
[cutaneous alternaria tenuissima alternariosis]. 19883202582
amino-acid composition and mycology of sekete, a fermented product from zea mays l.the local procedure for preparing sekete is described. the isolated microfungi, aspergillus flavus link., cladosporium cladosporioides (fres.) de vries, and alternaria tenuissima (kunze) wiltshire, were examined for various aspects of their physiology and biochemistry. sekete is rich in important amino acids as alanine, leucine, tyrosine, and lysine, though proline and arginine are lacking. all the mycoflora isolated exhibited good secretion of extracellular enzymes. pullulan, starch, soyabean m ...19883265987
two new cases of cutaneous alternariosis with a review of the literature.two cases of cutaneous phaeohyphomycosis due to alternaria tenuissima in patients affected with primitive myeloproliferative syndrome and lymphocytic lymphoma respectively are reported. following a review of the specific literature, the authors discuss the etiopathogenic mechanisms, the clinical and histologic aspects of the lesions and the therapeutic problems.19863540674
some enzymic properties of a protease from alternaria tenuissima. 19695391183
pilot-plant production of protease by alternaria tenuissima.agar slants of a selected alternaria tenuissima strain were illuminated to give conidia suitable for further propagation. for production of protease with an optimal caseolytic activity in the region of ph 8 to 10, the fungus was cultivated in steel fermentors with 6- and 60-liter working capacity. maximal activity, 1.5 enzyme units per liter, was attained in a medium based on liver after about 60 hr of cultivation. the protease was secreted parallel with, or slightly after, the main growth phase ...19676067633
survey of mycoflora and mycotoxins of some dried fruits in egypt.fifty-five species and two varieties appertaing to 23 genera were collected and identified from 4 samples of dried fig and 3 dried samples of each of apricot, plum and raisin. forty-nine species and two varieties belonging to 20 genera were isolated on 1% glucose-czapek's while 31 species and one variety belonging to 16 genera were isolated on 40% sucrose-czapek's agar medium at 28 +/- 2 degrees c. penicillium, aspergillus and cladosporium were the common genera on the two types of media used. a ...19938229671
dematiaceous fungi are an increasing cause of human disease.the dematiaceous fungi appear to be an increasing cause of human disease. at the methodist hospital, in houston, texas, five cases of serious disease caused by these fungi occurred between 1987 and 1992. cerebral abscesses with xylohypha bantiana followed treatment for lymphoma. an infection of the lower extremity with exophiala jeanselmei var. castellanii followed cardiac surgery. peritoneal growth of alternaria tenuissima was a complication of peritoneal dialysis. cerebral abscesses with dacty ...19968824969
antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents.the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ...200616989308
new feather-degrading filamentous fungi.among 106 filamentous fungi isolated from poultry farm waste, 13 species belonging to seven genera (aspergillus, acremonium, alternaria, beauvaria, curvularia, paecilomyces, and penicillium) were able to grow and produce keratinase in stationary cultures using poultry feather powder as the only substrate. the four most efficient keratinase producers were selected for a comparative study of keratinase production in submerged and stationary conditions. the highest keratinolytic activities were pro ...200817899245
sensitivity of the alternaria infectoria species-group 1 to cycloheximide.a possibility of using cycloheximide tolerance and/or sensitivity as an additional diagnostic tool for distinguishing morphologically related species within common small-spored alternaria has been tested during this study.200818444978
airborne fungal load in agricultural environment during threshing operations.the effect of threshing operations on the fungal spora of farm air, has been studied with the culture plate method. air samples were taken before, during and after the threshing of paddy and wheat grains, at the end of the respective crop seasons. an immediate local increase in the airborne fungal load was observed during threshing operations. in case of wheat the high rise was due to alternaria tenuissima, followed by drechslera sp. and cladosporium herbarum. in 'boro' variety of paddy, the hig ...19947808509
[alternaria tenuissima plurifocal cutaneous infection].multiple skin localization of phaeohyphomycoses are rare and are encountered in immunodepressed subjects. we report a case due to alternaria tenuissima in a patient given corticosteroids and iga deficiency.19957486720
[Extracellular expression of protein elicitor PeaT1 in Bacillus subtilis to enhance drought tolerance and growth in wheat].PeaT1, a protein elicitor from Alternaria tenuissima can promote plant growth and trigger systemic acquired resistance in plants. In order to expand the application of PeaT1, P43 promoter sequence and nprB signal peptide-encoding sequence were cloned from Bacillus subtilis 168 chromosomal DNA. The two sequences and peaT1 gene were spliced by overlapping extension. This product was cloned into the Escherichia coli-B. subtilis shuttle vector pHY300-PLK and the resultant recombinant expression vect ...201122117519
toxigenicity of fungi from grain sorghum.the mycoflora of nine varieties of grain sorghum was determined by plating serial dilutions of ground samples on rose bengal-streptomycin agar. seventeen species of fungi representing 10 genera were identified. curvularia, penicillium, mucor, and aspergillus were dominant genera. extracts of p. herquei were highly toxic to brine shrimp, while those of c. clavata, c. lunata, and mucor mucedo showed low to moderate toxicity. extracts of c. clavata, c. lunata, and m. mucedo were highly toxic to chi ...19817196500
purification and some properties of a protease from alternaria tenuissima. 19695812572
rna-seq derived identification of differential transcription in the chrysanthemum leaf following inoculation with alternaria tenuissima.a major production constraint on the important ornamental species chrysanthemum is black spot which is caused by the necrotrophic fungus alternaria tenuissima. the molecular basis of host resistance to a. tenuissima has not been studied as yet in any detail. here, high throughput sequencing was taken to characterize the transcriptomic response of the chrysanthemum leaf to a. tenuissima inoculation.201424387266
tenuazonic acid production by alternaria alternata and alternaria tenuissima isolated from cotton.cultures of alternaria alternata (three isolates) and alternaria tenuissima (three isolates) obtained from cottonseeds and bolls were toxigenic when cultured on various laboratory media. a mycotoxin was isolated and identified as tenuazonic acid by using solvent partition, thin-layer chromatography, and instrument analyses. toxicity was monitored with brine shrimp and chicken embryo bioassays. all cultures except a. alternata 938 produced tenuazonic acid when grown on cottonseed and on yeast ext ...1977562128
peat1-induced systemic acquired resistance in tobacco follows salicylic acid-dependent pathway.systemic acquired resistance (sar) is an inducible defense mechanism which plays a central role in protecting plants from pathogen attack. a new elicitor, peat1 from alternaria tenuissima, was expressed in escherichia coil and characterized with systemic acquired resistance to tobacco mosaic virus (tmv). peat1-treated plants exhibited enhanced systemic resistance with a significant reduction in number and size of tmv lesions on wild tobacco leaves as compared with control. the quantitative analy ...201021088909
in vitro cytotoxicity of fungi spoiling maize silage.penicillium roqueforti, penicillium paneum, monascus ruber, alternaria tenuissima, fusarium graminearum, fusarium avenaceum, byssochlamys nivea and aspergillus fumigatus have previously been identified as major fungal contaminants of danish maize silage. in the present study their metabolite production and in vitro cytotoxicity have been determined for fungal agar and silage extracts. all 8 fungal species significantly affected caco-2 cell viability in the resazurin assay, with large variations ...201120837087
alpha-amylase production by toxigenic fungi.this study is concerned with the screening of alternaria alternata (fr.) keissler and alternaria tenuissima (kunze ex pers.) wilts strains for the biosynthesis of alpha-amylases. nine strains of a. alternata and three strains of a. tenuissima were grown on enzyme production medium (epm) and potato dextrose agar (pda) using three ph levels (4.5-6.5); then the selected strains, able to produce bigger zones of starch hydrolysis on solid media, were subjected the testing of their amylolytic efficacy ...201020812132
mutation of alternaria tenuissima fcbp-252 for hyper-active alpha-amylase.production of extracellular alpha-amylase enzyme by a filamentous fungus, alternaria tenuissima was studied in solid-state fermentation (ssf) as well as submerged fermentation (smf). the potential strain was successfully mutated by uv and ethyl methanesulfonate (ems). high-level of alpha-amylase activity was obtained by the mutant at-ch-5.6 (76.75 units ml(-1)) after chemical treatment followed by uv mutant at-uv-2.8 (63.12 units ml(-1)) which was significantly higher than parental a. tenuissima ...200919761044
fungal endophyte diversity and bioactivity in the indian medicinal plant ocimum sanctum linn.endophytic mycopopulation isolated from india's queen of herbs tulsi (ocimum sanctum) were explored and investigated for their diversity and antiphytopathogenic activity against widespread plant pathogens botrytis cinerea, sclerotinia sclerotiorum, rhizoctonia solani and fusarium oxysporum. 90 fungal isolates, representing 17 genera were recovered from 313 disease-free and surface sterilised plant segments (leaf and stem tissues) from three different geographic locations (delhi, hyderabad and mu ...201526529087
synthesis and in vitro antifungal efficacy of oleoyl-chitosan nanoparticles against plant pathogenic fungi.an antifungal dispersion system was prepared by oleoyl-chitosan (o-chitosan) nanoparticles, and the antifungal activity against several plant pathogenic fungi was investigated. under scanning electron microscopy, the nanoparticles formulation appeared to be uniform with almost spherical shape. the particle size of nanoparticles was around 296.962 nm. transmission electron microscopy observation showed that nanoparticles could be well distributed in potato dextrose agar medium. mycelium growth ex ...201626434526
[expression of peat1 gene from alternaria tenuissima in pichia pastoris and its function].in this study, peat1 gene was subcloned into the pichia pastoris expression vector ppic9k, which contained both the methanol-inducible promoter and the transcription terminator of the aox1 gene, resulting the plasmid ppic9k-peat1. the recombinant plasmid was linearized by sal i or bgl ii and transformed into p. pastoris gs115 by electroporation method. recombinant strain was screened by minimal dextrose medium and further confirmed by pcr. the gene was in frame integrated into the pichia genome ...200919621583
identification and mycotoxigenic capacity of fungi associated with pre- and postharvest fruit rots of pomegranates in greece and cyprus.pre- and postharvest fruit rots of fungal origin are an important burden for the pomegranate industry worldwide, affecting the produce both quantitatively and qualitatively. during 2013, local orchards were surveyed and 280 fungal isolates from greece (gr) and cyprus (cy) were collected from pomegranates exhibiting preharvest rot symptoms, and additional 153 isolates were collected postharvest from cold-stored fruit in gr. molecular identification revealed that preharvest pomegranate fruit rots ...201526057112
secondary metabolites from a strain of alternaria tenuissima isolated from northern manitoba soil.deoxyphomalone (1), dimethyl 4-methyl-2,6-pyridinedicarboxylate (2), stemphyperylenol (3), and n-methyl-2-pyrrolidone (4) were isolated from the fermentation broth of a strain of the fungus, alternaria tenuissima. this fungus was isolated from the soil underlying the lichen, peltigera didactyla, which had been collected from wapusk national park in northern manitoba. the structures of the compounds were determined by comprehensive analysis of their spectroscopic data including ft-ir, 1d and 2d n ...201525920216
identification, characterization and mycotoxigenic ability of alternaria spp. causing core rot of apple fruit in greece.alternaria core rot is a major postharvest disease of apple fruit in several countries of the world, including greece. the study was conducted aiming to identify the disease causal agents at species level, investigate the aggressiveness of alternaria spp. isolates and the susceptibility of different apple varieties and determine the mycotoxigenic potential of alternaria spp. isolates from apple fruit. seventy-five alternaria spp. isolates obtained from apple fruit showing core rot symptoms were ...201525560914
1h and 13c nmr assignments of three new tricycloalternarenes from endophytic fungus alternaria tenuissima hs03-07. 201525295464
pyrone derivatives from the endophytic fungus alternaria tenuissima sp-07 of chinese herbal medicine salvia przewalskii.three new pyrones, solanapyrones p-r (1-3), were afforded by the extracts of the endophytic fungus alternaria tenuissima sp-07 isolated from the fresh root of chinese herbal medicine salvia przewalskii, along with the known solanapyrones (4-6) and benzopyrones (7-9). solanapyrones p (1) and q (2) possess an unprecedented nor-solanapyrone skeleton as natural products. their structures were determined on the basis of nmr and hr-esi-ms analysis. the plausible biosynthetic pathways to those unknown ...201425284429
identification and characterization of cdna sequences encoding the his3 and leu2 genes of the fungus alternaria tenuissima.alternaria tenuissima is a fungus widely present in the environment and could cause diseases in plants and humans. in this study, through a yeast genetic approach, cdna sequences were isolated and characterized for the athis3 and atleu2 genes. athis3 cdna encodes a protein of 238 amino acids, while atleu2 cdna encodes a protein of 363 amino acids. based on the phylogenetic analysis of amino acid sequences of athis3p and atleu2p, a. tenuissima is closely related to the plant pathogenic fungus pha ...200818439983
[expression of alternaria tenuissima peat2 gene in pichia pastoris and its function].a positive clone was screened from alternaria tenuissima expression library. the result of sequencing and gene analysis indicated that the cloned dna fragment has a complete orf, which was named peat2 (protein elicitor from alternaria tenuissima 2) with the genbank accession number ef212880. the gene was amplified by pcr and subcloned into the ppic9k of pichia pastoris expression system. the resulting recombinant plasmid ppic9k/peat2 was verified by sequencing and digested by sac i. the lineariz ...200717944356
effect of selected natural dyes in reduction on colour changes of egyptian linen textiles by fungi.linen is the most historical egyptian textile fibre liable to fungal deterioration. fungal deterioration of dyed linen textiles may appear as undesirable different stains. in order to success in removing of fungal stains from biodeteriorated historical egyptian dyed linen textiles, it is necessary to understand the nature and causes of these stains, hence their subsequent removal. so this paper aims to investigate the effect of fungi on dyed linen textiles. in this study linen textile samples we ...200717867537
isolation of cdna sequences encoding the map kinase hog1 and the map kinase kinase pbs2 genes of the fungus alternaria tenuissima through a genetic approach.alternaria tenuissima is a fungus widely present in the environment and causes diseases in plants and humans in the world. in this study, we constructed an a. tenuissima cdna expression library in a centromeric yeast vector that allows the isolation of functional cdna sequences from this environmental and pathogenic fungus. through a genetic approach we have isolated and functionally characterized the cdna sequences encoding the map kinase (mapk) hog1p and the mapk kinase pbs2p of a. tenuissima. ...200717306900
skin infection due to alternaria species in kidney allograft recipients: report of a new case and review of the literature.a kidney allograft recipient developed a cutaneous infection 29 months after transplantation, due to the dematiaceous fungus alternaria infectoria on his right forearm and left leg. since the lesions were too large to be excised, the patient was treated only with systemic itraconazole and a reduction of the immunosuppressive therapy. after 4 months, the lesions were completely healed, and no relapses were observed at follow-up of 22 months. twenty-seven other cases of cutaneous alternariosis hav ...200617136699
the major allergen of alternaria alternata (alt a 1) is expressed in other members of the pleosporaceae family.there is general consensus regarding the scarce cross-reactivity existing between alternaria alternata and other allergenic moulds such as aspergillus fumigatus, penicillium notatum or cladosporium herbarum. however, a. alternata has been shown to have a very significant level of allergenic cross-reactivity with other fungi belonging to the pleosporaceae family. to date, no biological identity or homologies with other proteins have been described for alt a 1, and it remains unclear whether the m ...200616466440
[determination of the profiles of secondary metabolites characteristic of alternaria strains isolated from tomato].many alternaria species have been studied for their ability to produce bioactive secondary metabolites, such as tentoxin (ten), some of which have toxic properties. the main food contaminant toxins are tenuazonic acid, alternariol (aoh), alternariol monomethyl ether (ame), altenuene, and altertoxins i, ii and iii.201424071643
oribatid mites as potential vectors for soil microfungi: study of mite-associated fungal species.the ability of soil-living oribatid mites to disperse fungal propagules on their bodies was investigated. classical plating methods were applied to cultivate these fungi and to study their morphology. molecular markers were used for further determination. the nuclear ribosomal large subunit and the nuclear ribosomal internal transcribed spacer of dna extracts of the cultured fungi as well as total dna extracts of the mites themselves, also containing fungal dna, were amplified and sequenced. bas ...200516333718
tricycloalternarene derivatives from endophytic fungus alternaria tenuissima sy-p-07.a new tricycloalternarene, tca 11a (1), was isolated from the culture broth of an endophytic fungus, alternaria tenuissima sy-p-07, along with four known structurally related compounds 2-5. the structure of compound 1 was elucidated by extensive spectroscopic methods, especially 2d nmr, and the absolute stereochemistry of 1 was suggested on the basis of the cd spectral analysis and noesy data.201323458246
shape evolution with temperature of a thermotolerant protein (peat1) in solution detected by small angle x-ray scattering.the protein elicitor from alternaria tenuissima (peat1) presented excellent thermotolerance and potential application in agriculture as a pesticide. previous synchrotron radiation circular dichroism study demonstrated that the secondary structures in peat1 protein are reversible with temperature change. to further clarify the mechanism of its thermotolerance, synchrotron radiation small angle x-ray scattering (saxs) technique was used to study the shape change of peat1 protein with temperature i ...201322890773
cocultivation of phytopathogenic fusarium and alternaria strains affects fungal growth and mycotoxin production.a laboratory study was conducted to evaluate the influence of cocultivation of toxigenic fusarium (f.) and alternaria (a.) fungi with respect to growth and mycotoxin production.201222776416
comparative chemical characterization of pigmented and less pigmented cell walls of alternaria tenuissima.alternaria tenuissima, the parasitic fungus, was obtained from the pruned upper-cut surfaces of mulberry stems. this fungus contains dark pigment because of the presence of melanin in the cell wall. to obtain less-pigmented cell walls, this fungus was grown under dark condition. when the pigmented and less-pigmented cell walls were chemically analyzed, no differences were observed in amino-acid composition, hexoses, or pentoses. however, in pigmented cell walls, higher contents of melanin (2.6%) ...200516252128
toxigenic alternaria species from argentinean blueberries.blueberries are traditionally consumed in north america, some european countries and japan. in argentina, the blueberry crop is profitable because production starts in november, when the northern hemisphere lacks fresh fruit. fungal contaminants can grow and produce mycotoxins in fresh fruit. the aims of this work were to identify the main genera of the mycobiota of blueberries grown in argentina and to determine the toxicogenic potential, pathogenicity and host specificity of the species isolat ...201222285534
cutaneous infection with alternaria tenuissima in an immunocompromised patient.a 28-year-old man, on immunosuppressive therapy for a recent kidney-pancreas transplant, presented to our department with an unusual violaceous nodule on his right knee. a biopsy specimen showed a granulomatous response in the dermis, surrounding numerous fungal cells and septate hyphae. fungal cultures yielded dark grey colonies, which were identified as alternaria tenuissima. to our knowledge this is the first report of a. tenuissima infection reported in the u.k.200516225623
physical and chemical aspects of long-term biodeterioration of some polymers and composites.a biodeterioration study was performed on synthetic polymeric materials including homogenous film made from poly(tetrafluorine ethylene), copolymer film made from tetrafluorine ethylene and perfluoromethyl vinyl ether, vulcanized rubber containing natural caoutchouc, and vulcanized rubber, the main component of which was synthetic butadiene nitrile caoutchouc. the materials were exposed for 12 years to the open air, in mycological containers, and in a cellar in maritime climate conditions: air h ...200415269902
influence of he-ne laser irradiation of soybean seeds on seed mycoflora, growth, nodulation, and resistance to fusarium solani.laser irradiation of soybean seeds for 3 min caused a clear reduction in the number of seed-borne fungi which became more pronounced as the irradiation time was extended. pretreatment of the seeds with methylene blue, methyl red and carmine enhanced the effect of laser. rhizoctonia solani, alternaria tenuissima, cercospora kikuchii and colletotrichum truncatum were completely eliminated when the seeds were pretreated with a dye and irradiated for 10 min. seed germination was stimulated on exposu ...199910983234
two cases of cutaneous phaeohyphomycosis by alternaria alternata and alternaria tenuissima.two cases of cutaneous phaeohyphomycosis, one with a nodular appearance and the other with an erythematous infiltrating patch, are reported in immunocompromised patients. diagnosis was based on histological examination, which revealed hyphae and round-shaped fungal cells in a granulomatous dermal infiltrate, and on identification of the moulds when biopsy fragments were cultured on sabouraud-dextrose agar without cycloheximide. the pathogens were alternaria tenuissima in the first case and a. al ...19979335148
cutaneous phaeohyphomycosis due to alternaria tenuissima.a 50-year-old gardener with a 3-year history of pemphigus vulgaris and steroid-induced diabetes complained of various papulonodular lesions on the left elbow. the lesions had appeared 11 months previously. skin biopsy showed chronic granulomatous inflammation of the dermis which contained septate hyphae and large spores mainly free. fragments of the biopsy specimen, cultured on sabouraud glucose agar without cycloheximide, for 4 days, produced soft white colonies which later turned blackish brow ...19968909032
stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by peat1.the protein elicitor from the mycelium of alternaria tenuissima has been isolated. the elicitor triggered resistance to the tobacco mosaic virus in tobacco by inducing relative oxygen species, but without causing hypersensitive necrosis. the elicitor is reported to impart resistance against verticillium dahliae and to increase yield in cotton, but its mechanism is not yet clear. in this study, the stable isotope labelled mass spectrometry method was used to quantify the relative abundances of pr ...201021181342
characterization of microbial communities and fungal metabolites on field grown strawberries from organic and conventional production.the background levels of culturable indigenous microbial communities (microbiotas) on strawberries were examined in a field survey with four conventional and four organic growers with different production practise and geographic distribution. the microbiota on apparently healthy strawberries was complex including potential plant pathogens, opportunistic human pathogens, plant disease biocontrol agents and mycotoxin producers. the latter group was dominated by penicillium spp. and aspergillus nig ...201223290240
cmwrky15 facilitates alternaria tenuissima infection of chrysanthemum.abscisic acid (aba) has an important role in the responses of plants to pathogens due to its ability to induce stomatal closure and interact with salicylic acid (sa) and jasmonic acid (ja). wrky transcription factors serve as antagonistic or synergistic regulators in the response of plants to a variety of pathogens. here, we demonstrated that cmwrky15, a group iia wrky family member, was not transcriptionally activated in yeast cells. subcellular localization experiments in which onion epidermal ...201526600125
the extracellular system of beta-1,3-glucanases of alternaria tenuissima and aspergillus vesicolor.during cultivation in a minimal medium with glucose alternaria tenuissima and aspergillus vesicolor produce constitutively alpha- and beta-glucanases. fractions of beta-1,3-glucanases exhibiting affinity for laminarin were separated by means of gel filtration chromatography. two neutral beta-1,3-glucanases with affinity for yeast glucan were isolated by affinity chromatography and further characterized.19807188760
cutaneous alternariosis.a 77-year-old woman had had a slowly spreading lesion of five years' duration on her left cheek. the lesion consisted of sharply demarcated, dark-red plaques with infiltration. a biopsy specimen from the lesion showed mixed-cell granulomatous infiltration in the upper to middle dermis. hyphae were observed in the granulomatous tissue. alternaria tenuissima was isolated from a biopsy specimen. antimycotic susceptibility test with amphotericin b, ketoconazole, and flucytosine revealed that the iso ...19883190258
first report of leaf spot caused by alternaria tenuissima on black chokeberry (aronia melanocarpa) in korea.in july 2015, diseased leaves of black chokeberry (aronia melanocarpa) were observed in danyang and gochang, korea. the symptoms appeared as circular or irregular brown leaf spots, from which alternaria tenuissima was isolated. the isolates were cultured on potato dextrose agar, and their morphological characteristics were observed under a light microscope. the colonies were whitish to ash colored. the pathogenicity test on healthy black chokeberry leaves produced circular brown spots, in line w ...201627790070
mycobiota of the weed conyza canadensis (asteraceae) in brazil.conyza canadensis is a noxious and notably problematic weed in brazil whose control is greatly challenging due to the appearance, in the last years, of herbicide resistant populations. an investigation regarding the associated pathogenic fungi of this plant was conducted in brazil aimed at starting a biological control program to help mitigate its economic impact. ten fungal species were yielded and described: two ascomycetes (leptosphaerulina conyzicola sp. nov. and wentiomyces melioloides), fi ...201627567718
homoharringtonine production by endophytic fungus isolated from cephalotaxus hainanensis li.homoharringtonine (hht), a natural plant alkaloid derived from cephalotaxus, has demonstrated to have a broad antitumor activity and efficacy in treating human chronic myeloid leukemia. an alternative source is required to substitute for the slow-growing and scarce cephalotaxus to meet the increasing demand of the drug market. the objective of this study was to screen hht-producing endophytic fungi from cephalotaxus hainanensis li. by screening 213 fungal isolates obtained from the bark parts of ...201627263005
endophytic fungus from sinopodophyllum emodi (wall.) ying that produces podophyllotoxin.the aryltetralin lactone podophyllotoxin, which exhibits pronounced antineoplastic activity, is used as the precursor of the following three clinical anticancer drugs: etoposide™, etopophos™ and teniposide™. the natural occurrence of this arylnaphthalene lignan is scarce and unable to meet the ever-rising demand in the medical industry. thus, developing alternative sources for the production of podophyllotoxin is extremely urgent. this is the first report of the production of podophyllotoxin fro ...201626306574
altertoxins with potent anti-hiv activity from alternaria tenuissima que1se, a fungal endophyte of quercus emoryi.screening of a small library of natural product extracts derived from endophytic fungi of the sonoran desert plants in a cell-based anti-hiv assay involving t-cells infected with the hiv-1 virus identified the etoac extract of a fermentation broth of alternaria tenuissima que1se inhabiting the stem tissue of quercus emoryi as a promising candidate for further investigation. bioactivity-guided fractionation of this extract led to the isolation and identification of two new metabolites, altertoxin ...201425260957
inducible and constitutive expression of an elicitor gene hrip1 from alternaria tenuissima enhances stress tolerance in arabidopsis.hrip1 is a novel hypersensitive response-inducing protein secreted by alternaria tenuissima that activates defense responses and systemic acquired resistance in tobacco. this study investigates the role that hrip1 plays in responses to abiotic and biotic stress using transgenic arabidopsis thaliana expressing the hrip1 gene under the control of the stress-inducible rd29a promoter or constitutive cauliflower mosaic virus 35s promoter. bioassays showed that inducible hrip1 expression in rd29a∷hrip ...201525120219
cutaneous alternariosis in a renal transplant recipient.alternariosis is a fungal infection that is usually described in immunocompromised patients. we report a case of cutaneous alternariosis in a renal transplant recipient caused by alternaria tenuissima. the diagnosis was supported by histopathologic (ie, yeastlike cells, filamentous structures) and mycologic findings from a cutaneous biopsy. cutaneous lesions regressed 1 month following a decrease in the dosage of immunosuppressive therapy. the patient also was treated with intravenous amphoteric ...201424897135
exploiting composting biodiversity: study of the persistent and biotechnologically relevant microorganisms from lignocellulose-based composting.the composting ecosystem is a suitable source for the discovery of novel microorganisms and secondary metabolites. this work analyzes the identity of microbial community that persists throughout lignocellulose-based composting, evaluates their metabolic activities and studies the capability of selected isolates for composting bioaugmentation. bacterial species of the phyla firmicutes, actinobacteria and proteobacteria and fungi of the phylum ascomycota were ubiquitous throughout the composting. ...201424759645
a new compound from an endophytic fungus alternaria tenuissima.a new secondary metabolite, named altertoxin iv (1), together with altertoxin ii (2), was isolated from the fermentation broth of alternaria tenuissima, an endophytic fungal strain residing in the stem of tribulus terrestris l. the structure of new compound 1 was established by hr-esi-ms, multinuclear nmr spectroscopy, and single crystal x-ray diffraction method. in their in vitro bioassay, compound 2 exhibited moderate cytotoxic activity against pc-3 cell lines with an ic50 value of 14.28 μm.201424660902
a mixed culture of endophytic fungi increases production of antifungal polyketides.secondary metabolites produced by endophytic microorganisms can provide benefits to host plants, such as stimulating growth and enhancing the plant's resistance toward biotic and abiotic factors. during its life, a host plant may be inhabited by many species of endophytes within a restrictive environment. this condition can stimulate secondary metabolite production that improves microbial competition and may consequently affect both the neighboring microorganisms and the host plant. the interact ...201324114180
identification and characterization of ethanol utilizing fungal flora of oil refinery contaminated soil.the indigenous fungal flora of three oil refinery contaminated sites (bharuch, valsad and vadodara) of india has been documented in the present investigation. a total seventy-five fungal morphotypes were isolated from these sites and out of them, only fifteen isolates were capable of utilizing ethanol (0-8%; v:v) as a sole source of carbon and energy for growth. ten percent ethanol was completely lethal for the growth of all the isolated fungus. biochemical characterization of the potent ethanol ...201424068535
hrip1, a novel protein elicitor from necrotrophic fungus, alternaria tenuissima, elicits cell death, expression of defence-related genes and systemic acquired resistance in tobacco.here, we report the identification, purification, characterization and gene cloning of a novel hypersensitive response inducing protein secreted by necrotrophic fungus, alternaria tenuissima, designated as hypersensitive response inducing protein 1 (hrip1). the protein caused the formation of necrotic lesions that mimic a typical hypersensitive response and apoptosis-related events including dna laddering. the protein-encoding gene was cloned by rapid amplification of cdna ends (race) method. th ...201222591019
genetic variability of brazilian isolates of alternaria alternata detected by aflp and rapd techniques.the alternaria brown spot (abs) is a disease caused in tangerine plants and its hybrids by the fungus alternaria alternata f. sp. citri which has been found in brazil since 2001. due to the recent occurrence in brazilian orchards, the epidemiology and genetic variability of this pathogen is still an issue to be addressed. here it is presented a survey about the genetic variability of this fungus by the characterization of twenty four pathogenic isolates of a. alternata f. sp. citri from citrus p ...200924031413
phenotypic and phylogenetic segregation of alternaria infectoria from small-spored alternaria species isolated from wheat in germany and russia.to identify the taxonomic differences between phytopathogenic small-spored alternaria strains isolated from wheat kernels in germany and russia by a polyphasic approach.201526381081
routes of phlogopite weathering by three fungal strains.fungi dissolve soil minerals by acidification and mechanical disruption. dissolution may occur at the microscale (contact between fungus and mineral) and medium scale (entire mineral grains). mineral weathering by fungi and other microorganisms is thought to be of significant global contribution, perhaps producing specific weathering signatures. we report fungal dissolution of phlogopite mica in experiments with three fungal strains (alternaria tenuissima, cladosporium cladosporioides, stilbella ...201627890092
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