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isolation, characterization, and identification of bacterial contaminants in semifinal gelatin extracts.bacterial contamination of gelatin is of great concern. indeed, this animal colloid has many industrial applications, mainly in food and pharmaceutical products. in a previous study (e. de clerck and p. de vos, syst. appl. microbiol. 25:611-618), contamination of a gelatin production process with a variety of gram-positive and gram-negative bacteria was demonstrated. in this study, bacterial contamination of semifinal gelatin extracts from several production plants was examined. since these extr ...200415184171
anaerobic growth of bacillus mojavensis and bacillus subtilis requires deoxyribonucleosides or dna.bacillus mojavensis strains jf-2 (atcc 39307), rob2, and abo21191(t) and bacillus subtilis strains 168 (atcc 23857) and atcc 12332 required four deoxyribonucleosides or dna for growth under strict anaerobic conditions. bacillus licheniformis strains l89-11 and l87-11, bacillus sonorensis strain tg8-8, and bacillus cereus (atcc 14579) did not require dna for anaerobic growth. the requirement for the deoxyribonucleosides or dna did not occur under aerobic growth conditions. the addition of a mixtu ...200415345407
bacillus aerius sp. nov., bacillus aerophilus sp. nov., bacillus stratosphericus sp. nov. and bacillus altitudinis sp. nov., isolated from cryogenic tubes used for collecting air samples from high altitudes.four novel bacterial strains were isolated from cryogenic tubes used to collect air samples at altitudes of 24, 28 and 41 km. the four strains, 24k(t), 28k(t), 41kf2a(t) and 41kf2b(t), were identified as members of the genus bacillus. phylogenetic analysis based on 16s rrna gene sequences indicated that three of the strains, 24k(t), 28k(t) and 41kf2a(t), are very similar to one another (>98 % sequence similarity) and show a similarity of 98-99 % with bacillus licheniformis and 98 % with bacillus ...200616825614
identification and safety evaluation of bacillus species occurring in high numbers during spontaneous fermentations to produce gergoush, a traditional sudanese bread snack.gergoush is a naturally fermented sudanese bread snack produced in three fermentation steps (primary starter, adapted starter and final dough), followed by three baking steps for a half to one hour at above 200 °c. this study examines the microbiota of two sets of fermentations performed at a traditional production site in khartoum, sudan in 2006 and 2009, respectively. in 2006 four different milk/legume based primary starters (faba bean, chick pea, lentil and white bean) were sampled in order t ...201121429611
bacterial associations across house fly life history: evidence for transstadial carriage from managed manure.house flies (diptera: muscidae; musca domestica l.) associate with microbe-rich substrates throughout life history. because larvae utilize bacteria as a food source, most taxa present in the larval substrate, e.g., manure, are digested or degraded. however, some species survive and are present as third-instar larvae begin pupation. during metamorphosis, many bacteria are again lost during histolysis of the larval gut and subsequent remodeling to produce the gut of the imago. it has been previous ...201626798138
quorum quenching bacillus sonorensis isolated from soya sauce fermentation brine.an n-acylhomoserine lactone (ahl)-degrading bacterial strain, l62, was isolated from a sample of fermentation brine of chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v). l62, a rod-shaped gram positive bacterium with amylolytic activity, was phylogentically related to bacillus sonorensis by 16s ribosomal dna and rpob sequence analyses. b. sonorensis l62 efficiently degraded n-3-oxohexanoyl homoserine lactone and n-octanoylhomoserine lactone. however, the aiia ho ...201222666018
[teichoic acids of three type strains of the "bacillus subtilis" group, bacillus mojavensis vkm b-2650, bacillus amyloliquefaciens subsp. amyloliquefaciens vkm b-2582, and bacillus sonorensis vkm b-2652].cell walls of three type strains of the "bacillus subtilis" group, bacillus mojavensis vkm b-2650, bacillus amyloliquefaciens subsp. amyloliquefaciens vkm b-2582, and bacillus sonorensis vkm b-2652, are characterized by the individual set of teichoic acids. all strains contained 1,3-poly(glycerophosphates), unsubstituted, acylated with d-alanine, and glycosylated. the latter differ in the nature of the monosaccharide residue. teichoic acids of b. mojavensis vkm b-2650(t) and b. amyloliquefaciens ...201425509395
screening for bacillus subtilis group isolates that degrade cyanogens at ph 4.5-5.0.cyanogenic food crops abound in nature with important crops like cassava forming the staple food for over half a billion people. detoxification by hydrolysis of cassava cyanogenic glycosides often involves acid fermentation, and in some of these processes bacillus species are encountered. forty bacillus spp. (20 bacillus subtilis, 11 bacillus licheniformis, 7 bacillus sonorensis, 2 bacillus cereus) isolated from acid fermented primary starters to produce gergoush, a sudanese fermented snack, wer ...201323261810
bacillus piscis sp. nov., a novel bacterium isolated from the muscle of the antarctic fish dissostichus mawsoni.in this paper, a new bacterial strain designated as 16mft21(t) is isolated from the muscle of a fish caught in the antarctic ocean. strain 16mft21(t) is a gram-staining-positive, catalase-oxidase-positive, rod-shaped facultative-aerobic bacterium. the phylogenetic analysis that is based on the 16s-rrna gene sequence of strain 16mft21(t) revealed that it belongs to the genus bacillus in the family bacillaceae in the class bacilli. the highest degrees of the sequence similarity of the strain 16mft ...201627888465
inhibition of listeria monocytogenes biofilms by bacteriocin-producing bacteria isolated from mushroom substrate.this study was designed to investigate the ability of naturally occurring bacteria isolated from mushroom substrate to prevent biofilm formation by listeria monocytogenes or to remove existing biofilms in mushroom production facilities.201727797439
characterization of a hyperthermostable alkaline lipase from bacillus sonorensis 4r.hyperthermostable alkaline lipase from bacillus sonorensis 4r was purified and characterized. the enzyme production was carried out at 80°c and 9.0 ph in glucose-tween inorganic salt broth under static conditions for 96 h. lipase was purified by anion exchange chromatography by 12.15 fold with a yield of 1.98%. the molecular weight of lipase was found to be 21.87 kda by sds-page. the enzyme activity was optimal at 80°c with t 1/2 of 150 min and at 90°c, 100°c, 110°c, and 120°c; the respective va ...201626904276
bioprocess development for the production of sonorensin by bacillus sonorensis mt93 and its application as a food preservative.media composition and environmental conditions were optimized using statistical tools, plackett burman design and response surface methodology, to maximize the yield of a bacteriocin, named as sonorensin, from a new marine isolate bacillus sonorensis mt93 showing broad spectrum of antimicrobial activity. under optimized conditions, mt93 produced 15-fold higher yield of sonorensin compared to that under initial fermentation conditions. as oxygen supply is a critical parameter controlling growth a ...201525459843
isolation and identification of allelochemicals produced by b. sonorensis for suppression of charcoal rot of arachis hypogaea l.bacillus sonorensis mbcu2 isolated from vermicompost-amended soil from gujarat, india showed most antagonistic activity against macrophomina phaseolina by dual culture screening. the culture supernatant of mbcu2 completely suppressed the mycelia growth of pathogen, indicating that suppression was due to the presence of allelochemicals in the culture filtrate. results of scanning electron microscopy revealed that mbcu2 caused morphological alteration in mycelia of m. phaseolina as evident by hyph ...201525346523
bioscouring of cotton using lipase from marine bacteria bacillus sonorensis.bioscouring refers to the enzymatic removal of impurities from cotton fabric, which imparts it with improved hydrophilicity for further wet processes. in the present study, the efficacy of lipase from newly isolated marine bacteria bacillus sonorensis isolated from marine clams paphia malabarica collected from kalbadevi estuary, mumbai, india, has been evaluated for scouring of cotton fabric and compared with conventional alkaline scouring of cotton. as a scouring agent for cotton fabrics, the l ...201525256798
sonorensin: an antimicrobial peptide, belonging to the heterocycloanthracin subfamily of bacteriocins, from a new marine isolate, bacillus sonorensis mt93.marine environments are the greatest fronts of biodiversity, representing a resource of unexploited or unknown microorganisms and new substances having potential applications. among microbial products, antimicrobial peptides (amps) have received great attention recently due to their applications as food preservatives and therapeutic agents. a new marine soil isolate producing an amp was identified as bacillus sonorensis based on 16s rrna gene sequence analysis. it produced an amp that showed a b ...201424610839
draft whole-genome sequence of bacillus sonorensis strain l12, a source of nonribosomal lipopeptides.the bacillus sonorensis l12 draft genome sequence is approximately 4,647,754 bp in size with a g+c content of 45.2%. over 86% of the genome contains protein-encoding genes, including several gene clusters for de novo biosynthesis of the nonribosomal lipopeptides iturin, bacitracin, and fengycin, which could mean that the strain exhibits antifungal effects.201323538904
biosynthesis and physicochemical characterization of a bacterial polysaccharide/polyamide blend, applied for microfluidics study in porous media.screening among some new isolated bacteria from oily samples, which were capable of producing extracellular polymeric substances (epss), one was selected and identified as bacillus sonorensis. an efficient micro-total analysis approach was carried out to assay the produced epss by this bacterium. sucrose and yeast concentrations as carbon and nitrogen sources, respectively, sodium salt concentration and initial ph were selected to be the variables in experimental design. production of eps in opt ...201727864059
a report on extensive lateral genetic reciprocation between arsenic resistant bacillus subtilis and bacillus pumilus strains analyzed using rapd-pcr.the study involves isolation of arsenic resistant bacteria from soil samples. the characterization of bacteria isolates was based on 16s rrna gene sequences. the phylogenetic consanguinity among isolates was studied employing rpob and gltx gene sequence. rapd-pcr technique was used to analyze genetic similarity between arsenic resistant isolates. in accordance with the results bacillus subtilis and bacillus pumilus strains may exhibit extensive horizontal gene transfer. arsenic resistant potency ...201727956257
antimicrobial susceptibility of bacillus strains isolated from primary starters for african traditional bread production and characterization of the bacitracin operon and bacitracin biosynthesis.bacillus spp. are widely used as feed additives and probiotics. however, there is limited information on their resistance to various antibiotics, and there is a growing concern over the transfer of antibiotic resistance genes. the mic for 8 antibiotics was determined for 85 bacillus species strains, bacillus subtilis subsp. subtilis (n = 29), bacillus licheniformis (n = 38), and bacillus sonorensis (n = 18), all of which were isolated from starters for sudanese bread production. all the strains ...201222941078
a novel eps-producing strain of bacillus licheniformis isolated from a shallow vent off panarea island (italy).a haloalkaliphilic, thermophilic bacillus strain (t14), isolated from a shallow hydrothermal vent of panarea island (italy), produced a new exopolysaccharide (eps). phylogenetic analysis based on 16s rrna gene sequences showed that strain t14 was highly related (99 % similarity) to bacillus licheniformis dsm 13(t) and bacillus sonorensis dsm 13779(t). further dna-dna hybridization analysis revealed 79.40 % similarity with b. licheniformis dsm 13(t) and 39.12 % with b. sonorensis dsm 13779(t). su ...201323397221
prevalence of thermoduric bacteria and spores on 10 midwest dairy farms.thermoduric bacteria (tdb), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. they have the potential to adversely affect the quality and shelf life of products. the objectives of this study were to identify the origin and common species of heat-resistant bacteria occurring during summer and winter on midwest dairy farms. bulk tank milk samples were taken from 10 dairy farms located along the south dakota section of interstate 29, with ...201425200773
corrigendum to "prevalence of thermoduric bacteria and spores on 10 midwest dairy farms".thermoduric bacteria (tdb), including sporeformers and their spores, can be present in milk and dairy products even after pasteurization. they have the potential to adversely affect the quality and shelf life of products. the objectives of this study were to identify the origin and common species of heat-resistant bacteria occurring during summer and winter on midwest dairy farms. bulk tank milk samples were taken from 10 dairy farms located along the south dakota section of interstate 29, with ...201425610925
isolation and genetic identification of spore-forming bacteria associated with concentrated-milk processing in nebraska.spore-forming bacteria are heat-resistant microorganisms capable of surviving and germinating in milk after pasteurization. they have been reported to affect the quality of dairy products by the production of enzymes (lipolytic and proteolytic) under low-temperature conditions in fluid milk, and have become a limiting factor for milk powder in reaching some selective markets. the objective of this research was to isolate and identify the population of spore-forming bacteria (psychrotrophic and t ...201727988120
isolation of promising bacterial strains from soil and marine environment for polyhydroxyalkanoates (phas) production utilizing jatropha biodiesel byproduct.phas are biodegradable and environmentally friendly thermoplastics. the major contributor to pha production cost is carbon substrate cost, therefore it is desirable to produce pha from waste/byproducts like jatropha biodiesel byproducts. this study was done using jatropha biodiesel byproduct as carbon source, to decrease production cost for phas. total 41 isolates from soil and marine source were able to utilize jatropha biodiesel byproduct. nine bacteria were selected for further studies, which ...201020417229
bacillus sonorensis sp. nov., a close relative of bacillus licheniformis, isolated from soil in the sonoran desert, arizona.eight bacillus strains isolated from sonoran desert soil were shown to belong to a previously unidentified species, for which the name bacillus sonorensis sp. nov. is proposed. the type strain is strain l87-10t (= nrrl b-23154t). on the basis of phenotypic and genetic data, b. sonorensis is most closely related to bacillus licheniformis. b. sonorensis can be distinguished from b. licheniformis by salt tolerance, pigmentation, multilocus enzyme electrophoresis, reassociation of genomic dna and se ...200111594594
bacillus paralicheniformis sp. nov., isolated from fermented soybean paste.an isolate of a gram-stain-positive, facultatively anaerobic, motile, rod-shaped, endospore-forming bacterium was recovered from soybean-based fermented paste. phylogenetic analysis of the 16s rrna gene indicated that the strain was most closely related to bacillus sonorensis kctc-13918t (99.5 % similarity) and bacillus licheniformis dsm 13t (99.4 %). in phenotypic characterization, the novel strain was found to grow at 15–60 °c and to tolerate up to 10 % (w/v) nacl. furthermore, the strain grew ...201526296568
bacillus glycinifermentans sp. nov., isolated from fermented soybean paste.two independent isolates of a gram-stain-positive, facultatively anaerobic, motile, rod-shaped bacterium were recovered from cheonggukjang, a korean fermented soybean paste food product. preliminary sequencing analysis of the 16s rrna gene indicated that these strains were related most closely to bacillus sonorensis kctc-13918t and bacillus licheniformis dsm 13t. in phenotypic characterization, the novel strains were found to grow between 15 and 55 °c and to tolerate up to 8 % (w/v) nacl. furthe ...201526297378
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