| purification and serological characterization of a type-specific antigen of streptococcus equisimilis. | a microtiter complement fixation (cf) procedure was developed for use in detection of antibodies in sera of swine and rabbits vaccinated with streptococcus equisimilis. crude ultrasonic as well as acid-extracted preparations contained cf antigen, but the ultrasonic procedure resulted in a higher yield of active antigen. evidence of serotype specificity with varying degrees of cross reactivity was detected with the cf procedure when representative strains of four different serotypes of s. equisim ... | 1975 | 53203 |
| septicemia due to streptococcus equisimilis in a child with acute lymphoblastic leukemia. | a 4-year-old boy with acute lymphoblastic leukemia in relapse with documented septicemia due to group c streptococci (streptococcus equisimilis) is described. the patient responded well to therapy with appropriate cytotoxic and antimicrobial agents. there is a general lack of recognition of the pathogenicity of group c streptococci in man. the potential opportunistic nature of these organisms in immunocompromised hosts and the need for early recognition and appropriate treatment of such infectio ... | 1979 | 291465 |
| epizootic streptococcal pneumonia in captive coyotes. | severe death loss was observed in captive coyotes, canis latrans. of 48 coyotes in one kennel, 22 (46%) died within a 7 day period. cause of death was determined to be acute fibrinopurulent (bacterial) broncho-pneumonia and pleuritis. streptococcus equisimilis was isolated from the lungs of two coyotes examined. | 1979 | 522217 |
| group c streptococcal surgical wound infections transmitted by an anorectal and nasal carrier. | streptococcus equisimilis (lancefield group c), an unusual cause of nosocomial surgical infection, was isolated from two orthopedic postoperative wound infections. both operations had been performed by the same surgeon within a three-day period. examination of the surgeon revealed perianal dermatitis from which s. equisimilis was isolated. the organism was also recovered from the surgeon's nose and rectum. the wound infections responded to treatment with penicillin derivatives. the surgeon was s ... | 1978 | 634676 |
| osteomyelitis due to streptococcus equisimilis (group c). | | 1979 | 761006 |
| immunogenicity of experimental streptococcus equisimilis vaccines in swine. | the protective and complement-fixing antibody immune responses to streptococcus equisimilis vaccines were evaluated in young, surgically derived, colostrum-deprived swine. comparable levels of protection against live s equisimilis challenge exposure developed in response to sonic-extract, acid-extract, and whole-cell vaccines combined with incomplete freund adjuvant. the extract vaccines induced higher levels of complement-fixing antibody than did killed, whole cells. protection, as well as comp ... | 1977 | 835866 |
| [immunoelectronmicroscopic localization of cell wall antigens in streptococci. ii. localization of the group-specific polysaccharide of group c streptococci with ferritin- and peroxidase-labelled helix pomatia-agglutinin (author's transl)]. | the carbohydrate of the cell wall of group c streptococci is one of the best known group-specific streptococcal antigens with respect to its chemical structure. in the present paper, the ultrastructural location of the carbohydrate was investigated by means of immunoelectronmicroscopic techniques. besides group-specific antibodies, the specific binding of an agglutinin (protectin anti-ahp) of the edible snail (helix pomatia) to structures with terminal n-acetylgalactosamine was used to demonstra ... | 1975 | 1154918 |
| group c streptococci: "streptococcus equisimilis" or streptococcus anginosus? | | 1992 | 1554852 |
| group-c beta-hemolytic streptococcal bacteremia. | group-c beta-hemolytic streptococci (gcbhs) is an uncommon cause of bacteremia. in a 5-year period, gcbhs accounted for 0.28% of positive blood cultures and 0.35% of bacteremias documented at our hospital. the incidence of gcbhs bacteremia was 0.05 episodes per 1000 admissions. we were able to analyze clinical data of 10 of the 13 patients with gcbhs bacteremias. all but one were adults with significant underlying diseases, and seven episodes were community acquired. the skin was the portal of e ... | 1992 | 1572140 |
| influence of age on neutrophil function in foals. | functional activities (phagocytosis and killing) of neutrophil leucocytes (nl) and immunoglobulin g concentrations were evaluated in six healthy foals from birth to 6 months of age. peripheral blood nl were reacted with streptococcus equisimilis in 20 per cent pooled equine serum for 30, 60 and 90 mins and functional activities of nl were determined using a fluorochrome microassay. values for foal nl function were compared with those of healthy adult horses (n = 28). foal neutrophil function was ... | 1991 | 1778167 |
| septic arthritis secondary to group c streptococcus typed as streptococcus equisimilis. | | 1991 | 1856828 |
| [comparison between the aged and children of the normal distribution of pharyngeal and intestinal hemolytic streptococci]. | a marked difference between the aged and children has been observed in the group distribution of hemolytic streptococci isolated from various clinical specimens. group b strains from the urine and sputum, and group g from the sputum, pus and exudate have been predominant in the aged, whereas group a strains from the throat swab have dominated in children. the present study was undertaken to clarify the background for such a marked difference by investigating the normal state of pharyngeal and in ... | 1991 | 1919133 |
| isolation, sequence and expression in escherichia coli, bacillus subtilis and lactococcus lactis of the dnase (streptodornase)-encoding gene from streptococcus equisimilis h46a. | a partial library of bcli-generated chromosomal dna fragments from streptococcus equisimilis h64a (lancefield group c) was constructed in escherichia coli. clones displaying either streptokinase or deoxyribonuclease (streptodornase; sdc) activities were isolated. the gene (sdc) expressing the sdc activity was allocated on the 1.1-kb acci dna subfragment. sequence analysis of this dna fragment revealed the presence of one open reading frame, which could encode a protein of 36.8 kda. the n-termina ... | 1991 | 1937032 |
| schonlein-henoch purpura and streptococcus equisimilis. | | 1991 | 1954147 |
| beta-haemolytic non-group a streptococci and pharyngitis: a case-control study. | a case-control study was conducted to examine the association of beta-haemolytic non-group a streptococci and pharyngitis in a paediatric population (n = 502). no association could be found between disease and traditional lancefield groups b, c, f, or g. when the analysis included the clustering of genetically related subgroups from different lancefield groups, an association was evident for moderate and heavy quantitations of beta-haemolytic "large colony" group c and g streptococci (streptococ ... | 1991 | 1959540 |
| beta-d-glucuronidase activity assay for rapid differentiation of species within beta-haemolytic group c and g streptococci. | methylumbelliferyl-conjugated enzyme substrates were assessed for their ability to differentiate beta haemolytic streptococci in lancefield groups c and g. both streptococcus equisimilis (group c) and large colony human biotype group g strains were consistently differentiated from group c and g "streptococcus milleri group" bacteria by their ability to hydrolyse the beta-d-glucuronide substrate. the test was completed in less than one hour. | 1991 | 1960215 |
| group c streptococcal bacteremia: analysis of 88 cases. | eighty-eight cases of group c streptococcal bacteremia were reviewed retrospectively. most patients had underlying diseases (72.7%), predominantly cardiovascular disease (20.5%) or malignancy (20.5%). the infection originated most often from the upper respiratory tract (20.5%), the gastrointestinal tract (18.2%), or the skin (17.1%). prior exposure to animals or animal products was reported in 23.9% of cases. the most common clinical manifestations of group c streptococcal bacteremia were endoca ... | 1991 | 2041960 |
| [empyema caused by streptococcus equisimilis]. | | 1990 | 2098127 |
| [aortic endocarditis caused by streptococcus equisimilis]. | | 1990 | 2098129 |
| [bacteremia caused by streptococcus equisimilis in a neoplastic patient treated with recombinant interleukin 2]. | | 1990 | 2098130 |
| group c streptococcal arthritis: case report and review. | streptococci account for approximately 15%-20% of cases of nongonococcal septic arthritis. the majority of these are due to group a streptococci, but group b and group g streptococci are being isolated more frequently. we present a case of group c streptococcal arthritis and summarize nine additional cases reported in the literature. the group c streptococci include the large colony of voges-proskauer-negative bacteria (streptococcus equi, streptococcus equisimilis, streptococcus zooepidemicus, ... | 1990 | 2237126 |
| duplication of the streptokinase gene in the chromosome of streptococcus equisimilis h46a. | the erythromycin resistance plasmid psm752 carrying the cloned streptokinase gene, skc, was introduced by protoplast transformation into streptococcus equisimilis h46a from which skc was originally cloned. cells transiently supporting the replication of psm752 gave rise to an erythromycin-resistant clone designated h46sm which was plasmid free and produced streptokinase at levels approximately twice as high as the wild type. southern hybridization of total cell dna with an skc-containing probe p ... | 1990 | 2283044 |
| an outbreak of puerperal fever caused by group c streptococci. | between 19 february and 18 april 1987, 33 confirmed cases of puerperal fever caused by streptococcus equisimilis serotype t204 occurred at three hospitals in and around chelmsford. most of the cases (70%) occurred on one ward, in which toilet seats and a shower are believed to have aided transmission, although insufficient data were obtained to exclude a role for person-to-person spread. possession of m-protein antigen was demonstrated in the outbreak strain. | 1989 | 2567764 |
| sequence-directed dna bending upstream of the streptokinase promoter. | a 450-base pair (bp) hinfi restriction fragment from the chromosome of streptococcus equisimilis h46a contains the early coding region of the streptokinase gene (skc), the skc promoter, and a stretch of dna 5' to the--35 region of the skc promoter. two-dimensional polyacrylamide (pa) gel electrophoresis at two different temperatures showed that this fragment migrates anomalously slowly on pa gels, suggesting the existence of a bent dna conformation. inspection of the nucleotide sequence confirme ... | 1989 | 2625666 |
| group c streptococci in human infection: a study of 308 isolates with clinical correlations. | a collection of 308 clinical isolates of beta-haemolytic lancefield group c streptococci was assembled from laboratories in england, nigeria and new zealand. of these, 276 isolates were streptococcus equisimilis, 23 s. milleri and nine s. zooepidemicus. isolates of s. equisimilis in the african collection, though few, gave higher rates of lactose and raffinose fermentation, aesculin hydrolysis and positive alpha-galactosidase reactions than those from elsewhere. erythromycin resistance was found ... | 1989 | 2737252 |
| outbreaks of human infection caused by pyogenic streptococci of lancefield groups c and g. | within a collection of 749 strains of group c streptococci (streptococcus equisimilis) and 2348 of group g, isolated over a period of 6 years, there were clusters from 63 probable outbreaks of infection. these occurred in burns and maternity units, hospital wards, outpatient clinics, army camps and religious communities. overall, it was possible to identify t-protein antigens in 88% of the group c strains and in 82% of group g. serotyping by the identification of t-protein antigens was a useful ... | 1989 | 2746629 |
| the presence of m proteins in outbreak strains of streptococcus equisimilis t-type 204. | cultures of streptococcus equisimilis (lancefield group c) from three outbreaks of illness were found to carry the t-protein antigen 204. strains of this type were not otherwise represented in a collection of 743 cultures of these 'pyogenes-like' streptococci isolated from other outbreaks of infection or as random isolates. two of the three outbreaks were of pharyngitis. the third arose in a maternity unit where the organism was isolated from mothers with puerperal fever, from staff and also fro ... | 1989 | 2809234 |
| extraction of group c streptococcal igg-binding receptor and characterization of the active peptides by western blotting and isoelectric focusing. | three different enzymatic extraction procedures were tested for their effectivity in releasing igg-binding peptides from the streptococcal cell wall. streptococcus equisimilis strain 12628 isolated from pig was incubated with phage-associated lysin, trypsin and streptomyces globisporus lytic enzyme and the extracts were investigated by western blotting, indirect erythrocyte agglutination and ouchterlony diffusion for igg-binding activity. with all treatments igg-binding peptides were extracted. ... | 1987 | 2960105 |
| nucleotide sequence of the streptokinase gene from streptococcus equisimilis h46a. | the entire nucleotide sequence of a cloned 2568-bp psti fragment from the genome of streptococcus equisimilis h46a encoding the streptokinase gene (skc) has been determined. the longest open reading frame comprises 1320 bp which code for streptokinase. the protein is synthesized with a 26-amino acid residue n-terminal extension having properties characteristic of a signal peptide. comparison of the deduced amino acid sequence with the available amino acid sequence of a commercial streptokinase r ... | 1985 | 2989113 |
| variations in binding of mammalian fibrinogens to streptococci groups a, b, c, e, g and to staphylococcus aureus. | twenty-eight beta-hemolytic streptococci of groups a, b, e, g and streptococcus equisimilis as well as four staphylococcus aureus strains were tested for their ability to bind fibrinogen preparations from different animal species: homo, baboon, rabbit, rat, guinea-pig, dog, horse, pig, cow and sheep. the patterns of binding indicated differences in the structures of the bacterial fibrinogen receptors. there were higher binding levels in streptococci groups a, g, and s. equisimilis than in repres ... | 1985 | 2990156 |
| tripartite streptokinase gene fusion vectors for gram-positive and gram-negative procaryotes. | a specific 1,596 bp hincii fragment ('skc) from the chromosome of streptococcus equisimilis contains an active streptokinase (sk) gene (skc) lacking, in addition to the expression signals, codons 1 through 39 of wild-type skc but retaining the remainder of the skc coding sequence together with the transcription terminator. using this fragment as an indicator gene, we constructed two types of vectors which in appropriate hosts resulted in the synthesis of sk fusion proteins after insertional acti ... | 1988 | 3043172 |
| expression of the streptokinase gene from streptococcus equisimilis in bacillus subtilis. | the previously cloned and sequenced streptokinase gene (skc) from streptococcus equisimilis h46a was inserted into plasmid vectors capable of replication in bacillus subtilis. the skc gene was expressed by use of its own transcription and translation signals which appeared to meet the stringent requirements of b. subtilis for efficient foreign gene expression. the secreted streptokinase activity began to decline toward the end of the exponential growth phase suggesting that b. subtilis exoprotea ... | 1986 | 3090231 |
| group c streptococcal osteomyelitis. | a case of hematogenous osteomyelitis due to a group c streptococcus (streptococcus equisimilis) is presented because of the unusual nature of the infecting organism. group c streptococci are common pathogens in animals but are infrequent causes of human infections. of therapeutic concern is the observation that these isolates are frequently penicillin-tolerant. for this reason, it is important to speciate beta-hemolytic streptococcal isolates and to perform antimicrobial susceptibility studies. | 1986 | 3519677 |
| human fc(gamma) receptors for differentiation in throat cultures of group c "streptococcus equisimilis" and group c "streptococcus milleri". | the biochemical characteristics and the presence of human fc(gamma) receptors of 52 throat isolates of group c beta-hemolytic streptococci were examined. among these isolates, 38 were identified as "streptococcus milleri" and 14 were identified as "streptococcus equisimilis." the differentiation of group c "s. equisimilis" from "s. milleri" with identical group antigens was easy to perform by the measurement of the size of the hemolytic zone on a sheep blood agar plate in an anaerobic atmosphere ... | 1986 | 3771760 |
| incidence and characterization of beta-hemolytic streptococcus milleri and differentiation from s. pyogenes (group a), s. equisimilis (group c), and large-colony group g streptococci. | the biochemical characteristics of 172 clinical isolates of group a, c, f, or g or "nongroupable" beta-hemolytic streptococci were examined. among these isolates, 91 were identified as beta-hemolytic strains of streptococcus milleri. the remaining isolates included 20 streptococcus pyogenes, 21 streptococcus equisimilis, 37 large-colony group g streptococci, and 3 unidentified nongroupable isolates. a majority (84%) of the s. milleri strains possessed lancefield group antigen (3 a, 27 c, 41 f, a ... | 1985 | 3902878 |
| neonatal sepsis and meningitis due to streptococcus equisimilis. | | 1985 | 4080589 |
| antigenic type-specificity of swine isolates of streptococcus equisimilis. | isolates of a lancefield group c organism, streptococcus equisimilis, of sow reproductive tract and young swine joint origin were found to possess type-specific antigens. capillary precipitin and immunodiffusion tests, done with acid extracts and cross-absorbed rabbit antisera, revealed four serotypes among 62 of 64 isolates. extracts of two swine isolates of s. equisimilis and other lancefield group c strains including s. equi, s. zooepidemicus and three previously established types of s. equis ... | 1972 | 4114978 |
| brain abscess due to streptococcus equisimilis in a maltworker. | | 1971 | 5544801 |
| influence of streptococcus equisimilis on sites of endochondral ossification in swine. | | 1967 | 5624591 |
| electron microscopy of procine synovial membrane cell layer in streptococcus equisimilis arthritis. | | 1969 | 5775621 |
| evaluation of a selective medium for isolation of haemophilus pleuropneumoniae. | crystal violet, lincomycin, spectinomycin and bacitracin were evaluated as selective agents in media for isolation of haemophilus pleuropneumoniae. no single antimicrobial agent or combination of two or more inhibited all non-haemophilus strains (escherichia coli, pasteurella haemolytica, pasteurella multocida, streptococcus faecalis, streptococcus equisimilis and staphylococcus aureus) without marked suppression of 16 h. pleuropneumoniae strains. a medium containing 1 micrograms/ml of crystal v ... | 1983 | 6230143 |
| surface receptors for serum albumin in group c and g streptococci show three different types of albumin specificity. | a total of 100 bacterial strains were tested for binding uptake of radiolabeled albumin preparations from 15 mammalian species. three types of surface structures with specific binding sites for albumin were defined. a previously described receptor for albumin was separated into type a in streptococcus equisimilis strains and in human group g streptococcal strains and type b in bovine group c streptococci. a new type of albumin receptor, type c, was found in streptococcus dysgalactiae strains, th ... | 1982 | 6295942 |
| binding of human fibronectin to group a, c, and g streptococci. | a total of 387 bacterial strains belonging to 35 species were tested in direct binding experiments for the uptake of purified radiolabeled human fibronectin. positive binding was found in group a, c, and g streptococci and in staphylococcus aureus. the group c streptococcal species streptococcus equisimilis, streptococcus zooepidemicus, streptococcus equi, and streptococcus dysgalactiae were uniformly reactive with fibronectin. beta-hemolytic bovine group g streptococci showed the same degree of ... | 1983 | 6299959 |
| streptokinase: cloning, expression, and excretion by escherichia coli. | genomic dna from streptococcus equisimilis strain h46a was cloned in escherichia coli by using the bacteriophage lambda replacement vector l47 and an in vitro packaging system. a casein/plasminogen overlay technique was used to screen the phage bank for recombinants carrying the streptokinase gene ( skc ). the gene was present with a frequency of 1 in 836 recombinants, and 10 independent clones containing skc were isolated and physically characterized. one recombinant clone was used to subclone ... | 1984 | 6374659 |
| expression of a streptokinase gene from streptococcus equisimilis in streptococcus sanguis. | using recombinant dna techniques, we introduced a previously cloned streptokinase gene from streptococcus equisimilis into the challis strain of s. sanguis (group h). the gene was expressed in the new host under the control of its own promoter and the gene product had biological properties identical to authentic streptokinase. however, the molecular weight of cloned streptokinase (42 k) as expressed by s. sanguis was substantially lower than that of authentic streptokinase (47 k). since the clon ... | 1984 | 6593564 |
| streptococcus equisimilis (group c) as a cause of ophthalmic infections. | the first two cases of human ophthalmic infections due to streptococcus equisimilis (group c) are reported. this organism is known to produce severe infections in animals, but infection in humans is rare. the distinction between beta-hemolytic group c streptococci and group a and their ability to cause disease in susceptible patients is discussed. | 1983 | 6600876 |
| demonstration of a new type of immunoglobulin g receptor in streptococcus zooepidemicus strains. | forty-seven bacterial strains representing four different group c streptococcal species were tested for binding of human and bovine immunoglobulin g (igg) subclasses. specific binding sites for igg were found in all bacterial species studied. the four species included differed, however, in their capacities to interact with various igg subclasses, indicating the existence of different types of igg receptors. streptococcus equisimilis and streptococcus dysgalactiae were found to carry the same typ ... | 1980 | 6769810 |
| the serotyping of hospital strains of streptococci belonging to lancefield group c and group g. | a collection of more than 300 strains belonging to either lancefield group c or group g was made. the cultures had been isolated either sporadically from patients with serious disease or as apparent clusters from various nosocomial outbreaks. t-protein antigens were sought. so far, nine distinct serotypes have been found among the group g streptococci and seven serotypes amongst the streptococcus equisimilis (group c) strains. of the sixteen serotypes, four were the original t-types 7, 16, 20 an ... | 1983 | 6822729 |
| streptococcus equisimilis pneumonia in a compromised host. | a fatal case of streptococcus equisimilis pneumonia and septicemia is described in a young man with hodgkin's disease. the disease course consisted of exudative pharyngitis, macular rash, septic shock, disseminated intravascular coagulation, deep vein thrombosis, and pulmonary embolization. s. equisimilis was isolated from blood, throat, and sputum cultures antemortem and from lung cultures at autopsy. | 1983 | 6833489 |
| [streptococcus equisimilis as a cause of bacteremia in 2 patients]. | | 1983 | 6888148 |
| influence of bacterial products on the motility of stallion spermatozoa. | sterile filtrates were prepared from equine isolates of klebsiella pneumoniae (genitalium), proteus mirabilis, pseudomonas aeruginosa, escherichia coli, streptococcus zooepidemicus, streptococcus equisimilis, actinobacillus equuli, and corynebacterium equi and mixed individually with extended stallion semen. when diluted in the extended semen the filtrates represented bacterial populations of 0.5 x 10(6), 1 x 10(6), 2 x 10(6) and 4 x 10(6) cells/ml. ph values were recorded for each filtrate. spe ... | 1982 | 6962871 |
| group c streptococcal pneumonia: report of a fatal case and review of the literature. | a previously healthy 22-year-old man acquired tonsillitis due to streptococcus equisimilis, a group c streptococcus. he rapidly developed pneumonia, empyema, septicemia, and metastatic arthritis, and despite appropriate therapy with antibiotics and drainage of abscesses, he experienced progressive respiratory insufficiency and died. the four species of group c streptococci are described in terms of their biochemical properties, the infections they cause in animals, and their tendency to produce ... | 1980 | 7012990 |
| bacteriologic study of sow agalactia. | necropsy of 13 agalactic and 11 normally lactating sows at 1 to 2 days after parturition revealed that 7 of the agalactic and 4 of the clinically normal sows had lesions of mastitis. escherichia coli, streptococcus equisimilis, and staphylococcus epidermidis were the predominant organisms isolated. the organisms were isolated as pure and as mixed cultures and all 3 were isolated from normal appearing, as well as mastitic, glands. pure culture isolation of e. coli (44 gland segments) was signific ... | 1981 | 7025714 |
| meningitis caused by streptococcus equisimilis (group c). | | 1982 | 7089635 |
| [purification and characterization of streptococcal hyaluronate lyase (author's transl)]. | hyaluronate lyase of group c strain h 46a (streptococcus equisimilis) was purified and characterized by isoelectric focusing, sodium-dodecylsulfate-acrylamide electrophoresis, polyacrylamide-gradient-electrophoresis and crossed immunoelectrophoresis. the purification of the hyaluronate lyase was performed successively by adsorption on florisil, chromatography on deae-cellulose, and - for separation of streptokinase - stirring with cpg-pore-glass. the last step was sepharose 6b. purified hyaluron ... | 1981 | 7269855 |
| comparison of mics of ceftiofur and other antimicrobial agents against bacterial pathogens of swine from the united states, canada, and denmark. | the mics of ceftiofur and other antimicrobial agents, tested for comparison, for 515 bacterial isolates of pigs from the united states, canada, and denmark with various diseases were compared. the organisms tested included actinobacillus pleuropneumoniae, escherichia coli, pasteurella multocida, salmonella choleraesuis, salmonella typhimurium, streptococcus suis, streptococcus dysgalactiae subsp. equisimilis, streptococcus equi subsp. equi, and streptococcus equi subsp. zooepidemicus. in additio ... | 1995 | 7494042 |
| transcription termination of the streptokinase gene of streptococcus equisimilis h46a: bidirectionality and efficiency in homologous and heterologous hosts. | in streptococcus equisimilis h46a, a hypersymmetrical transcription terminator with bidirectional activity was localized between the translational termination codons of the streptokinase gene, skc, and the rel-orf1 genes. these two transcription units are oriented towards each other, and under normal conditions the skc mrna level exceeds that of the rel-orf1 genes by a factor of at least 1000. reporter vectors based on the promoterless cat gene were constructed by transcriptional fusion of skc t ... | 1995 | 7531815 |
| secretion of streptokinase fusion proteins from escherichia coli cells through the hemolysin transporter. | the hemolysin (hlya) secretion system was used to achieve the sec-independent secretion of streptokinase (skc) originating from streptococcus equisimilis into the medium by escherichia coli cells. the in-frame fusions of the skc gene, either possessing or lacking a region encoding the signal peptide (sp) with the 3'-end of the hlya gene of various lengths were analysed. all hybrids retained skc activity. hybrid proteins devoided of the n-terminal sp, regardless of length of the hlya secretion si ... | 1995 | 7557478 |
| report of cases of and taxonomic considerations for large-colony-forming lancefield group c streptococcal bacteremia. | traditionally, group c streptococci include four species: streptococcus equisimilis, s. zooepidemicus, s. equi, and s. dysgalactiae, the first three of which are group c beta-hemolytic streptococci (gcbhs). however, many of the beta-hemolytic streptococci carrying lancefield group c antigen isolated from clinical specimens are s. milleri. these organisms can be differentiated by colony size. we retrospectively collected data concerning large-colony-forming gcbhs bacteremia that occurred during a ... | 1995 | 7559958 |
| complex transcriptional control of the streptokinase gene of streptococcus equisimilis h46a. | on the streptococcus equisimilis h46a chromosome, the divergent coding sequences of the genes for the plasminogen activator streptokinase (skc) and a leucine-rich protein (lrp), the function of which is unknown, are separated by a 328 bp intrinsically bent dna region rich in at tracts. to begin to understand the expression control of these two genes, we mapped their transcriptional initiation sites by s1 nuclease analysis and studied the influence of the bent intergenic region on promoter streng ... | 1995 | 7616967 |
| [susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine and combinations of these compounds]. | using a broth microdilution technique, the in vitro susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine, and combinations of these compounds was determined. the bacterial strains (n = 88) isolated recently from horses with respiratory symptoms belonged to the following species: streptococcus equi subsp. zooepidemicus (n = 34), streptococcus equi subsp. equi (n = 22), staphylococcus aureus (n = 9), klebsiella pneumoniae (n = 7), rh ... | 1995 | 7624856 |
| [thrombophlebitis of the cavernous sinus secondary to polysinusitis. report of a new case]. | cavernous sinus thrombophlebitis syndrome is uncommon nowadays. despite the wide range of antimicrobial agents it carries a high mortality. the disease frequently arises from infection of face, mouth, nose and paranasal sinuses, reaching the cavernous sinus by venous spread. involvement of adjacent anatomic structures accounts for most of the clinical findings. meningeal involvement, bilateral orbital edema and ocular cranial nerves palsy are very helpful in the differential diagnosis. streptoco ... | 1995 | 7734168 |
| binding of tissue-type plasminogen activator (t-pa) to neisseria meningitidis and haemophilus influenzae. | forty-nine bacterial strains representing five species known to interact with human plasminogen were tested for the ability to bind the two major human plasminogen activators, t-pa and urokinase. the bacterial species tested included haemophilus influenzae, neisseria meningitidis, streptococcus pyogenes, streptococcus equisimilis and human group g streptococci. all n. meningitidis and 11 of 14 h. influenzae strains displayed substantial binding of t-pa with values in the range of 20-46%. on the ... | 1994 | 7804168 |
| an outbreak of group c streptococcal infection in a maternity unit. | an outbreak of group c streptococcal infection (streptococcus equisimilis serotype t204) occurred in a maternity unit in durham over a 2-week period. the outbreak strain possessed an m-protein antigen identical to that found in a previously reported maternity unit outbreak. seven patients in total were affected, and in two patients infected perineal wounds took several weeks to heal. the source of the outbreak appeared to be a patient who developed a perineal wound infection and who subsequently ... | 1994 | 7806866 |
| characterization of a novel streptokinase produced by streptococcus equisimilis of non-human origin. | streptokinases are proteins with plasminogen activator activity produced by certain hemolytic streptococci. we previously identified equine streptococcal isolates which produced streptokinases (esks) that bound both human and equine plasminogen but only readily activated equine plasminogen (14). this property was exploited to purify a representative esk produced by streptococcus equisimilis strain 87-542-w. affinity chromatography with human plasminogen resulted in the isolation of a m(r) approx ... | 1994 | 7878639 |
| concurrent infection with streptococcus equisimilis and leishmania in a dog. | | 1994 | 7948211 |
| a fibronectin-binding protein from streptococcus equisimilis: characterization of the gene and identification of the binding domain. | we have cloned and characterized a gene, fnb, from s. equisimilis, encoding a fibronectin-binding protein, fnb. a genomic library containing chromosomal dna from s. equisimilis strain se165 in the puc18 vector in e. coli tg1 was screened using a dna fragment of the gene fnbb from s. dysgalactiae strain s2 as probe. the complete gene was sequenced. the molecular mass of the protein, calculated from the deduced amino acid sequence is 120 kda, which coincides with that determined by gel electrophor ... | 1994 | 7975149 |
| isolation of streptococcus equisimilis from abscesses detected in specific pathogen-free mice. | | 1994 | 7983853 |
| biochemical differences among human and animal streptococci of lancefield group c or group g. | pyogenic streptococci of lancefield group c or group g from human or animal sources were examined with a view to increasing the number of diagnostic tests useful for their differentiation. human strains of group g produced l-prolyl-l-arginine aminopeptidase but isolates of streptococcus equisimilis (group c) did not. tests for alpha-l-glutamate aminopeptidase together with fermentation of glycogen or sorbitol distinguished s. dysgalactiae from strains of s. equisimilis isolated from animals. it ... | 1994 | 8046738 |
| inactivation of the streptokinase gene prevents streptococcus equisimilis h46a from acquiring cell-associated plasmin activity in the presence of plasminogen. | the streptokinase gene of streptococcus equisimilis h46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. the results showed that h46a binds both the zymogen and active enzyme, generates surface-associated plasmin activity in the presence of plasminogen when producing streptokinase, and express ... | 1994 | 8132150 |
| bacteraemia due to different groups of beta-haemolytic streptococci: a two-year survey and presentation of a case of recurring infection due to streptococcus "equisimilis". | we present a 2-year survey of bacteraemic episodes due to beta-haemolytic streptococci and a case of recurring infection due to group c streptococcus, streptococcus "equisimilis". we found 53 episodes of bacteraemia with beta-haemolytic streptococci. group a was the most common, followed by groups b, g and c. the proportion of nosocomial cases was the same in all four groups i.e. 24% (neonatal cases excluded). the clinical picture presented by groups c and g streptococcal cases was indistinguish ... | 1993 | 8132363 |
| [endocarditis caused by streptococcus equisimilis affecting a prosthetic valve]. | | 1993 | 8142514 |
| a comparison of whole cell protein profiles for sporadic human isolates of streptococcus equisimilis and beta-haemolytic group g streptococci. | whole cell protein profiles were resolved for streptococcus equisimilis (group c) and large colony human biotype beta-haemolytic group g streptococci by the use of one dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis. strains of s. equisimilis (27 in toto) were distributed among eight patterns designated a to h. pattern a represented 48.2% of the latter isolates. strains of group g streptococci (59 in toto) were distributed among sixteen patterns designated 1-16, and there ... | 1994 | 8159124 |
| immunodominant antigens of streptococcus equisimilis shared by other beta-haemolytic streptococci. | three immunodominant antigens of streptococcus equisimilis (lancefield group c) with approximate mol. wts of 46, 66 and 105 kda were recognised by human serum igg and iga immunoblotting. these antigens were identified consistently by various human sera but immunoblots with iga (heavy chain) and secretory iga (j chain) from human respiratory secretions gave more variable results. antigens with similar migration rates were demonstrated in s.pyogenes, large colony human biotype group g streptococci ... | 1994 | 8176719 |
| genetic organization of the streptokinase region of the streptococcus equisimilis h46a chromosome. | the complete nucleotide sequences of four genes and one open reading frame (orf1) adjacent to the streptokinase gene, skc, from streptococcus equisimilis h46a were determined. these genes are encoded on the opposite dna strand to skc and are arranged as follows: dexb-abc-lrp-skc-orf1-rel. the dexb gene, coding for an alpha-glucosidase (m(r) 61,733), and abc, encoding an abc transporter (m(r) 42,080), are similar to the dexb and msmk genes, respectively, from the multiple sugar metabolism operon ... | 1993 | 8232196 |
| the histidyl-trna synthetase from streptococcus equisimilis: overexpression in escherichia coli, purification, and characterization. | we describe the high-level expression of the streptococcus equisimilis histidyl-trna synthetase gene (hiss) in escherichia coli and the purification and characterization of the gene product. due to a lack of an efficient e. coli ribosome binding sequence in the hiss gene, the coding region was fused in-frame to the expression vector pt7-7, thereby creating a fusion gene construct (pt7-7reciii), which is under the control of a strong bacteriophage t7 promoter. another construct (pt-7recii) was us ... | 1993 | 8248028 |
| distribution of presumptive pathogenicity factors among beta-hemolytic streptococci isolated from ethiopia. | beta-hemolytic streptococci are known to bind several mammalian proteins, which are presumed to be important in pathogenicity. the distribution of such binding structures was examined for mouse albumin, human serum iga, human igg, human fibrinogen, and human plasminogen. a total of 218 group a beta-hemolytic streptococci (gas) were studied: 5 isolates from children with acute rheumatic fever (arf), 18 from acute post-streptococcal glomerulonephritis (apsgn), 57 from tonsillitis, 52 from skin inf ... | 1993 | 8323739 |
| in-vitro susceptibility to antimicrobial drugs of bacterial isolates from horses in the netherlands. | minimum inhibitory concentrations (mics) of 30 antimicrobial agents (including the hitherto unreported antimicrobial agents doxycycline, minocycline, vanomycin, 3 quinolones and 3 combinations of antimicrobial agents) for isolates of salmonella spp. (20), escherichia coli (17), klebsiella spp. (8), proteus spp. (7), pseudomonas aeruginosa (7), actinobacillus equuli (5), rhodococcus equi (4), streptococcus zooepidemicus (23), streptococcus equisimilis (6), streptococcus equi (4), coagulase-positi ... | 1993 | 8354217 |
| abdominal pain associated with an umbilical abscess in a llama. | a 3-month-old llama with a presenting complaint of lethargy, anorexia, and a painful, distended abdomen was evaluated. the llama had intermittently strained to defecate during the 3 days prior to admission. physical examination results, hematologic data and lateral abdominal radiographs were used to diagnose a large umbilical abscess, which was causing a partial obstruction of the gastrointestinal tract. under general anesthesia, 3 liters of purulent exudate were drained from the abscess. the ab ... | 1993 | 8417858 |
| molecular cloning, sequence, structural analysis and expression of the histidyl-trna synthetase gene from streptococcus equisimilis. | the histidyl-trna synthetase gene (hiss) from streptococcus equisimilis was cloned and sequenced. the gene for this aminoacyl-trna synthetase has an open reading frame of 1278 nucleotides. the deduced amino acid sequence encodes a protein of 426 amino acids with mw = 47,932. the protein is predicted to be soluble with a pl = 5.27. the protein sequence has extensive overall identity/similarity with the escherichia coli and the yeast histidyl-trna synthetases (approximately 58% and approximately 2 ... | 1993 | 8441673 |
| role of beta-hemolytic group c streptococci in pharyngitis: incidence and biochemical characteristics of streptococcus equisimilis and streptococcus anginosus in patients and healthy controls. | the biochemical characteristics and the isolation rates of the two lancefield group c streptococcal species (s. equisimilis and s. anginosus) from patients with pharyngitis and asymptomatic controls were compared. some 239 strains of beta-hemolytic group c streptococci were isolated from 1,480 patients (209 strains) with pharyngitis and 227 controls (30 strains). a total of 44 strains displayed broad hemolysis, were voges-proskauer test negative, and produced glucuronidase. some 72.7% of these s ... | 1993 | 8463390 |
| role of group c beta-hemolytic streptococci in pharyngitis: epidemiologic study of clinical features associated with isolation of group c streptococci. | all lancefield group c beta-hemolytic streptococci isolated over 12 months from college students with clinical pharyngitis and age-matched healthy controls were identified. clinical features of upper respiratory tract infection and pyogenic pharyngitis as well as colony counts were tabulated for each patient according to throat culture results. of 1,480 patients, lancefield group c streptococcus equisimilis was isolated from 45 (3%) patients and streptococcus anginosus ("streptococcus milleri") ... | 1993 | 8463391 |
| etiology and pathology of equine placentitis. | placentas from aborted, stillborn, and premature foals were examined during the 1988 and 1989 foaling seasons, and 236 of 954 (24.7%) had placentitis. microorganisms associated with placentitis were isolated or demonstrated from 162 of 236 (68.6%) placentitis cases. leptospira spp. and a nocardioform actinomycete were 2 important, newly emerging bacteria associated with equine placentitis. major pathogens identified in decreasing order were streptococcus zooepidemicus, leptospira spp., escherich ... | 1993 | 8466982 |
| purification and characterization of streptolysin o secreted by streptococcus equisimilis (group c). | streptolysin o (slo) was purified from culture supernatants of group c streptococci. the final product was either the complete, native molecule (slon [pi, 6.0]) with the n-terminal sequence (asp)-ser-asn-lys-gln-asn-thr-ala-asn-thr-glu-thr- or a large fragment (slof [pi, 7.3]) with the n-terminal sequence ala-pro-lys-glu-met-pro-leu-glu-ser-ala-glu-lys-glu-glu-lys-. | 1993 | 8500915 |
| conservation of the organization of the streptokinase gene region among pathogenic streptococci. | using ten gene-specific probes from the cloned and sequenced streptokinase gene (skc) region (8,931 bp) of streptococcus equisimilis h46a, a human serogroup c strain, the conservation of these genes and their linkage relationships were studied by southern hybridization in pathogenic streptococci differing taxonomically, serologically, in regard to their host range, and in the class of plasminogen activator produced. the results indicate that in s. pyogenes (strains a374, nz131 and sf130/13) and ... | 1995 | 8577315 |
| the streptokinase gene: allelic variation, genomic environment and expression control. | the genes for streptokinase, the most important prokaryotic plasmingoen activator, exhibit allelic variation predominantly due to the polymorphism of an internal 220-base pair fragment that divides the phylogenetic tree of their products into two primary branches. current molecular genetic research seeks functional correlates of the allelic variation, aims at analyzing the genomic environment of the streptokinase gene, skc, and focuses on understanding its expression. of the six genes cloned and ... | 1995 | 8586173 |
| functional analysis of a rela/spot gene homolog from streptococcus equisimilis. | we examined the functional attributes of a gene encountered by sequencing the streptokinase gene region of streptococcus equisimilis h46a. this gene, originally called rel, here termed rels. equisimilis, is homologous to two related escherichia coli genes, spot and rela, that function in the metabolism of guanosine 5',3'-polyphosphates [(p)ppgpp]. studies with a variety of e. coli mutants led us to deduce that the highly expressed rel s. equisimilis gene encodes a strong (p)ppgppase and a weaker ... | 1996 | 8631718 |
| a rela/spot homologous gene from streptomyces coelicolor a3(2) controls antibiotic biosynthetic genes. | a 0.972-kilobase pair dna fragment from streptomyces lividans that induces the production of the blue-pigmented antibiotic actinorhodine in s. lividans when cloned on a multicopy plasmid has led to the isolation of a 4-kilobase pair dna fragment from streptomyces coelicolor containing homologous sequence. computer-assisted analysis of the dna sequence revealed three putative open reading frames (orfs), orf1, orf2, and orf3. orf2 extends beyond the sequenced dna fragment, and its deduced product ... | 1996 | 8631867 |
| importance of staphylococcus hyicus ssp hyicus as a cause of arthritis in pigs up to 12 weeks of age. | lame pigs, up to 12 weeks of age, were necropsied to establish a diagnosis. of 175 pigs examined, 165 were confirmed to have arthritis by histopathological examination of joint tissues. lesions were most common in the elbow and tarsal joints and least common in the joints of the feet. typically, there was severe fibrinopurulent inflammation of synovial membranes regardless of the bacteria isolated. a bacterial aetiology was found in 114 (69%) of the 165 pigs. in arthritic pigs in which an aetiol ... | 1996 | 8660240 |
| cloning, sequencing and functional overexpression of the streptococcus equisimilis h46a gapc gene encoding a glyceraldehyde-3-phosphate dehydrogenase that also functions as a plasmin(ogen)-binding protein. purification and biochemical characterization of the protein. | we previously identified dna sequences involved in the function of the complex promoter of the streptokinase gene from streptococcus equisimilis h46a, a human serogroup c strain known to express this gene at a high level. as a prerequisite to understanding possible mechanisms that control the balance between the plasminogen activating and plasmin(ogen) binding capacities of h46a, we describe here its gapc gene encoding glyceraldehyde-3-phosphate dehydrogenase (grap-dh, ec 1.2.1.12), a glycolytic ... | 1996 | 8706717 |
| structural dissection and functional analysis of the complex promoter of the streptokinase gene from streptococcus equisimilis h46a. | the overlapping tandem promoters of the streptokinase gene, p1 and p2, identified previously by s1 nuclease transcript mapping were functionally dissected by mutagenesis of their -10 regions and fused transcriptionally with or without the 202-bp upstream region (usr) to the luciferase reporter gene (luc) from photinus pyralis to analyze the contribution of the different sequence elements to promoter activity in escherichia coli and the homologous streptococcus equisimilis strain h46a. in e. coli ... | 1996 | 8803948 |
| cloning of heterologous genes specifying detrimental proteins on puc-derived plasmids in escherichia coli. | a system is described that enables the cloning of genes specifying detrimental proteins in escherichia coli. the system is based on puc plasmids and was developed for the expression of the bacillus subtilis csaa gene, which is lethal when expressed at high levels. suppressor strains that tolerate the presence of plasmids for high-level expression of csaa were isolated, which contained small cryptic deletion variants of the parental plasmid in high copy numbers. the cryptic plasmids consisted mai ... | 1996 | 8804394 |
| the domain organization of streptokinase: nuclear magnetic resonance, circular dichroism, and functional characterization of proteolytic fragments. | streptococcus equisimilis streptokinase (sk) is a bacterial protein of unknown tertiary structure and domain organization that is used extensively to treat acute myocardial infarction following coronary thrombosis. six fragments of sk were generated by limited proteolysis with chymotrypsin and purified. nmr and cd experiments have shown that the secondary and tertiary structure present in the native molecule is preserved within all fragments, except the n-terminal fragment sk7. nmr spectra demon ... | 1996 | 8845759 |
| protein f2, a novel fibronectin-binding protein from streptococcus pyogenes, possesses two binding domains. | binding of the group a streptococcus (gas) to respiratory epithelium is mediated by the fibronectin (fn)-binding adhesin, protein f1. previous studies have suggested that certain gas strains express fn-binding proteins that are different from protein f1. in this study, we have cloned, sequenced, and characterized a gene (prtf2) from gas strain 100076 encoding a novel fn-binding protein, termed protein f2. insertional inactivation of prtf2 in strain 100076 abolishes its high-affinity fn binding. ... | 1996 | 8858591 |
| localization of the sequence-determined dna bending center upstream of the streptokinase gene skc. | dna sequences upstream of the core promoter region of the streptokinase gene (skc) from streptococcus equisimilis h46a increase skc transcription more than tenfold in vivo. this promoter upstream region contains a segment of intrinsically bent dna, the precise location of which was determined experimentally by circular permutation analysis and theoretically by computer prediction. electrophoretic analysis of circularly permuted upstream dna fragments placed the bend center approximately at posit ... | 1996 | 8929127 |
| thermal stability of the three domains of streptokinase studied by circular dichroism and nuclear magnetic resonance. | streptococcus equisimilis streptokinase (sk) is a single-chain protein of 414 residues that is used extensively in the clinical treatment of acute myocardial infarction due to its ability to activate human plasminogen (plg). the mechanism by which this occurs is poorly understood due to the lack of structural details concerning both molecules and their complex. we reported recently (parrado j et al., 1996, protein sci 5:693-704) that sk is composed of three structural domains (a, b, and c) with ... | 1996 | 8976567 |
| purification of streptodornase from streptococcus equisimilis and its dna-induced conformational change. | extracellular streptodornase was purified from fermentation media of streptococcus equisimilis by stepwise carboxymethyl-sepharose column chromatography. the active enzyme fraction was eluted with phosphate buffer containing 0.2 m nacl. the purified enzyme showed a homogeneity on sds-page and had a subunit molecular weight of 35 kda. conformational change of streptodornase by binding to calf thymus dna was examined by circular dichroism (cd). cd study clearly showed a dna-induced conformational ... | 1997 | 9020028 |
| conformation and stability of streptokinases from nephritogenic and nonnephritogenic strains of streptococci. | conformation and stability of three sks from streptococcus equisimilis strain h46a, streptococcus pyogenes strain a374, and streptococcus pyogenes strain at27 were compared by limited proteolysis, cd, and fluorescence measurements and by dsc. the general similarity of the peptide cd spectra in the spectral region 185 to 260 nm indicates the same type of folding for the three proteins. fluorescence and aromatic cd spectra are consistent with a predominant surface localization of the aromatic amin ... | 1997 | 9037709 |
| [streptococcus equisimilis as the etiologic agent of endocarditis]. | | 1996 | 9044650 |
| characterization of the stringent and relaxed responses of streptococcus equisimilis. | the 739-codon rel(seq) gene of streptococcus equisimilis h46a is bifunctional, encoding a strong guanosine 3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (ppgppase) and a weaker ribosome-independent atp:gtp 3'-pyrophosphoryltransferase [(p)ppgpp synthetase]. to analyze the function of this gene, (p)ppgpp accumulation patterns as well as protein and rna synthesis were compared during amino acid deprivation and glucose exhaustion between the wild type and an insertion mutant carrying a rel(seq) ge ... | 1997 | 9098065 |