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a survey of gnathostoma larvae in fresh water fish in the valley of the yangtze river and morphological characteristics of the recovered larvae.investigations of the prevalence of larval gnathostomes in fresh water fishes were carried out at the southeastern yangtze valley, people's republic of china, in the periods of october 1989 and november 1990. fishes were collected from shanghai, chenchiang, nanching, chiuchiang and nanchang districts in 1989. additional sampling in shanghai district was done at kunshan, tien-shanfu, chingpu and nanhui. species of fishes collected were channa argus (110), siniperca chuatsi (24) and silurus asotus ...19969185266
complete genome analysis of the mandarin fish infectious spleen and kidney necrosis iridovirus.the nucleotide sequence of the infectious spleen and kidney necrosis virus (isknv) genome was determined and found to comprise 111,362 bp with a g+c content of 54.78%. it contained 124 potential open reading frames (orfs) with coding capacities ranging from 40 to 1208 amino acids. the analysis of the amino acid sequences deduced from the individual orfs revealed that 35 of the 124 potential gene products of isknv show significant homology to functionally characterized proteins of other species. ...200111878882
new emergy indices for sustainable development.the emergy indices for the evaluation of system's sustainable development ability were studied. results indicated that the emergy indices are simplified and merged, and a new emergy index for sustainable development (eisd) is deduced. employing eisd, two cases are conducted. the first one is to compare three different dike-pond agro-ecological engineering modes, which are: melon-melon-cabbage-four domestic fishes (mode i), melon-melon-cabbage-pig-four domestic fishes (mode ii) and melon-melon-ca ...200312974321
molecular cloning of the viperin gene and its promoter region from the mandarin fish siniperca chuatsi.a viperin gene has been cloned from the mandarin fish (siniperca chuatsi). from the first transcription initiation site, the mandarin fish viperin gene extends 3163 nucleotides to the end of the 3' untranslated region, and it contains six exons and five introns. the open reading frame of the viperin transcript has 1062 nucleotides which encode a 354 amino acid peptide. the amino acid sequence of mandarin fish viperin shows high identities with its homologues in teleosts and mammals except for th ...200415350746
the residual dynamic of polycyclic aromatic hydrocarbons and organochlorine pesticides in fishponds of the pearl river delta, south china.hong kong and south china are the most developed regions within china. the industrialization in these areas has resulted in severe environmental problems. sediment and biotic samples including tilapia (oreochromis mossambicus), bighead carp (aristichthys nobilis), grass carp (ctenopharyngodon idellus), crucian carp (carassius auratus) and mandarin fish/fresh water grouper (siniperca chuatsi) were collected from different fishponds in the pearl river delta (tanzhou, sanjiao, guangzhou, shipai, ch ...200515899281
gene structure of an antimicrobial peptide from mandarin fish, siniperca chuatsi (basilewsky), suggests that moronecidins and pleurocidins belong in one family: the piscidins.the gene of piscidin, an antimicrobial peptide, has been cloned from the mandarin fish, siniperca chuatsi. from the first transcription initiation site, the mandarin fish piscidin gene extends 1693 nucleotides to the end of the 3' untranslated region and contains four exons and three introns. a predicted 79-residue prepropeptide consists of three domains: a signal peptide (22 aa), a mature peptide (22 aa) and a c-terminal prodomain (35 aa). the shortage of xqq motif in the prodomain of mandarin ...200717498177
molecular characterization of neuropeptide y gene in chinese perch, an acanthomorph fish.the full-length neuropeptide y (npy) cdna of chinese perch siniperca chuatsi was 704 bp in length, and contained a 300 bp open reading frame encoding a prepro-npy with 99 amino acids. the predicted prepro-npy peptide contained a putative signal peptide of 28 amino acids and a mature npy of 36 amino acids, followed by the proteolytic processing site gly-lys-arg and 35 amino acids that comprise the c-terminal peptide of npy. amino acid alignment and phylogenetic analysis indicate that the predicte ...200717537656
genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional orf.the complete genome of mandarin fish siniperca chuatsi rhabdovirus (scrv) was cloned and sequenced. it comprises 11,545 nucleotides and contains five genes encoding the nucleoprotein n, the phosphoprotein p, the matrix protein m, the glycoprotein g, and the rna-dependent rna polymerase protein l. at the 3' and 5' termini of scrv genome, leader and trailer sequences show inverse complementarity. the n, p, m and g proteins share the highest sequence identities (ranging from 14.8 to 41.5%) with the ...200818068257
metal concentrations of common freshwater and marine fish from the pearl river delta, south china.sediments and fish, including tilapia (oreochromis mossambicus), bighead carp (aristichthys nobilis), grass carp (ctenopharyngodon idellus), and mandarin fish (siniperca chuatsi) were collected from different fish ponds in the pearl river delta (tanzhou, sanjiao, guangzhou, shipai, changan, and mai po) for the analysis of metalloids and heavy metals [arsenic (as), cadmium (cd), chromium (cr), mercury (hg), and lead (pb)]. the pollution of as in pond sediments was great; however, as in the edible ...200818080794
development and characterization of monoclonal antibodies to spring viraemia of carp virus.five monoclonal antibodies (mabs) against spring viraemia of carp (svcv0504, isolated from common carp in china) were produced from mice immunized with purified virus preparations. the virion of svcv contains five structural proteins, representing the nucleoprotein (n), phosphoprotein (p), matrix protein (m), glycoprotein (g) and rna-dependent rna polymerase (l). western blotting analysis revealed that three mabs (1h5, 1e10, and 1h7) recognized specifically to a single protein of 47kda (n), the ...200818378003
development of a mandarin fish siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (isknv).infectious spleen and kidney necrosis virus (isknv) is a typical species of the genus megalocytivirus in the family iridoviridae. however, until recently, no suitable piscine cell line is stably susceptible to isknv. here, a continuous cell culture derived from the mandarin fish fry (mff-1) was developed and has been subcultured over 60 passages during a period of 18 months. mff-1 consists predominantly of epithelial-like cells and grows well in dmem supplemented with 10% fetal bovine serum. mff ...200818485510
pepsinogens and pepsins from mandarin fish (siniperca chuatsi).four pepsinogens (pg-i, pg-ii, pg-iii(a), and pg-iii(b)) were highly purified from the stomach of the freshwater fish mandarin fish (siniperca chuatsi) by ammonium sulfate fractionation, anion exchange, and gel filtration. the molecular masses of the four purified pgs were 36, 35, 38, and 35 kda, respectively. all the pepsinogens converted into their active form pepsins within a few minutes under ph 2.0. the optimum ph and temperature of the four enzymes were 3.0-3.5 and 45-50 degrees c, using h ...200818543931
molecular cloning of two c1q-like cdnas in mandarin fish siniperca chuatsi.c1q, a subunit of the c1 complex, plays a key role in the recognition of immune complexes to initiate the classical complement pathway. in this study, we reported two c1q-like cdnas from mandarin fish (siniperca chuatsi), mc1q-like-1 (mc1ql1) and mc1q-like-2 (mc1ql2). the full-length cdna of mc1ql1was 990bp, containing a 71bp 5'-untranslated region (utr), an open reading frame (orf) of 723bp, and a 196bp long 3'-utr. mc1ql2 cdna was 1193bp, containing a 100bp 5'-utr, followed by an orf of 756bp ...200818571244
risk assessment of pcdd/fs levels in human tissues related to major food items based on chemical analyses and micro-erod assay.nine groups of food items (freshwater fish, marine fish, pork, chicken, chicken eggs, leafy, non-leafy vegetables, rice and flour) and three types of human samples (human milk, maternal serum and cord serum) were collected for the analysis of pcdd/fs. results of chemical analysis revealed pcdd/fs concentrations (pg g(-1) fat) in the following ascending order: pork (0.289 pg g(-1) fat), grass carp (ctenopharyngodon idellus) (freshwater fish) (0.407), golden thread (nemipterus virgatus) (marine fi ...200919535140
risk assessment of residual ddts in freshwater and marine fish cultivated around the pearl river delta, china.six species of freshwater fish collected from 10 fishponds in shunde and zhongshan, china, four species of marine fishes collected from different mariculture farms [four in hong kong (tung lung chau, ma wan, cheung chau and kat o) and two in mainland china (daya bay and shenzhen)] together with feed (both trash fish and commercial pellets) and sediment were analyzed for ddts. total ddts in freshwater fish flesh decreased in the order of: carnivores [1742 microg/kg lipid weight (l.w.)] > herbivor ...201019603131
[cdna cloning and expression characterization of three regulatory subunits of nadph oxidase within cytoplasm from mandarin fish, siniperca chuatsi].the phagocyte nadph oxidase plays a crucial role in host defense against invading microorganisms by catalyzing the formation of reactive oxygen species, which is the precursor of a variety of microbicidal oxidants such as hydrogen peroxide (h2o2). in the present study, full-length cdnas of three regulatory subunits of nadph oxidase, including p40phox, p47phox, p67phox were cloned from head kidney of mandarin fish utilizing the reverse transcription polymerase chain reaction and rapid amplificati ...201020672412
molecular cloning of ikkβ from the mandarin fish siniperca chuatsi and its up-regulation in cells by isknv infection.the iκb kinase β (ikkβ) plays crucial roles in regulating activation of nuclear factor-kappa b (nf-κb) in response to proinflammatory factors and microbial and viral infections. here, we report the cloning of an ikkβ cdna (named sicikkβ) from the mandarin fish siniperca chuatsi. the full-length cdna is 4052bp and contains an orf that encodes a predicted protein of 743-amino acid residues. the deduced amino acid sequence of sicikkβ has the same domain organization as human ikkβ, which consists of ...201120817314
vp15r from infectious spleen and kidney necrosis virus is a non-muscle myosin-ii-binding protein.vp15r protein, encoded by the 15th open reading frame of infectious spleen and kidney necrosis virus (isknv), was identified. vp15r is a 263-residue protein that is first transcribed within 12 h post-infection. the vp15r mrna is transcribed beginning at isknv genomic coordinate 12111, extending 167 bp upstream of the initiation codon. no signal peptides, transmembrane fragments, or nuclear localization signal sequences were predicted in the vp15r sequence. the 102-202 sequence of vp15r is homolo ...201020882305
characterization and expression analysis of a goose-type lysozyme from the rock bream oplegnathus fasciatus, and antimicrobial activity of its recombinant protein.lysozyme (muramidase) represents an important defense molecule of the fish innate immune system. known for its bactericidal properties, lysozyme catalyzes the hydrolysis of β-(1,4)-glycosidic bonds between the n-acetyl glucosamine and n-acetyl muramic acid in the peptidoglycan layer of bacterial cell walls. in this study, the complete coding sequence of a g-type lysozyme (rbglyz) was identified in the oplegnathus fasciatus rock bream fish genome by means of multi-tissue normalized cdna pyroseque ...201021167286
global landscape of structural proteins of infectious spleen and kidney necrosis virus.infectious spleen and kidney necrosis virus (isknv), the type species of the genus megalocytivirus in the family iridoviridae, causes severe damage to mandarin fish cultures in china. little is known about the proteins of isknv virions. in this study, a total of 38 isknv virion-associated proteins were identified by four different workflows with systematic and comprehensive proteomic approaches. among the 38 identified proteins, 21 proteins were identified by the gel-based workflows (one-dimensi ...201121209107
identification and functional characterization of an interferon regulatory factor 7-like (irf7-like) gene from orange-spotted grouper, epinephelus coioides.interferon regulatory factor (irf) 7 plays a crucial role in modulating cellular responses to viral infection and cytokines, including interferons (ifns). in the present study, a novel irf7 gene (designated as ecirf7) was cloned and characterized from orange-spotted grouper, epinephelus coioides. the full-length ecirf7 cdna is composed of 2089 bp and encodes a polypeptide of 433 amino acids with 81% identity to irf7 of siniperca chuatsi, and the genomic dna of ecirf7 consists of 9 exons and 8 in ...201121295068
involvement of caveolin-1 in the jak-stat signaling pathway and infectious spleen and kidney necrosis virus infection in mandarin fish (siniperca chuatsi).caveolae, the major source of caveolin-1 protein, are specialized invaginated microdomains of the plasma membrane that act as organizing centers for signaling molecules in the immune system. in the present study, we report the cloning and characterization of caveolin-1 (mcav-1) from mandarin fish (siniperca chuatsi) and study on the roles of mcav-1 in the fish jak-stat signaling pathway and in virus infection. the cdna sequence of mcav-1 was 707bp in size, encoding a protein of 181 amino acids, ...201121296425
Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus.Infectious spleen and kidney necrosis virus (ISKNV), belonging to the genus Megalocytivirus in the family Iridoviridae, is one of the major agents causing mortality and economic losses to the freshwater fish culture industry in Asian countries. Currently, little information regarding the antigenic properties of Megalocytivirus (especially ISKNV) is available. Our previous study using four different workflows with systematic and comprehensive proteomic approaches led to the identification of 38 I ...201121888976
infectious spleen and kidney necrosis virus (a fish iridovirus) enters mandarin fish fry cells via caveolae-dependent endocytosis.infectious spleen and kidney necrosis virus (isknv) is the type species of the genus megalocytivirus from the family iridoviridae. megalocytiviruses have been implicated in more than 50 fish species infections and currently threaten the aquaculture industry, causing great economic losses in china, japan, and southeast asia. however, the cellular entry mechanisms of megalocytiviruses remain largely uncharacterized. in this study, the main internalization mechanism of isknv is investigated in mand ...201122171272
two hsp90 genes in mandarin fish siniperca chuatsi: identification, characterization and their specific expression profiles during embryogenesis and under stresses.hsp90 plays important roles in multiple cellular stress responses. here, two cytoplasmic hsp90 isoforms, schsp90α and schsp90β, were identified from siniperca chuatsi. their cdna and gdna structures, amino acid sequence features, and sequence identities and phylogenetic analysis with other species were described. their expression profiles during embryonic development in different tissues and under stressful conditions were analyzed using real-time quantitative pcr. during embryogenesis, transcri ...201626820141
a novel antimicrobial peptide derived from fish goose type lysozyme disrupts the membrane of salmonella enterica.in aquaculture, accumulation of antibiotics resulted in development of resistance among bacterial pathogens. consequently, it became mandatory to find alternative to synthetic antibiotics. antimicrobial peptides (amps) which are described as evolutionary ancient weapons have been considered as promising alternates in recent years. in this study, a novel antimicrobial peptide had been derived from goose type lysozyme (lyzg) which was identified from the cdna library of freshwater fish channa stri ...201526477736
expression pattern, promoter activity and bactericidal property of β-defensin from the mandarin fish siniperca chuatsi.β-defensin (bd) are cysteine-rich, cationic antimicrobial peptides which play an important role in innate immune system against invading microbes. in the present study, the cdna cloning, expression analysis, transcriptional regulation and antimicrobial activity of β-defensin (scbd) from mandarin fish (siniperca chuatsi) were characterized. the cdna sequence of scbd is 596 bp which encodes a protein of 63 amino acids (aa). the scbd gene comprises three exons and two introns. the signal peptide is ...201222705342
comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish siniperca chuatsi.heat shock cognate protein 70 (hsc70) is a molecular chaperone that plays essential roles in maintaining the cellular protein homeostasis. in this study, two hsc70 isoforms were identified and characterized from mandarin fish siniperca chuatsi. they have similar sequence structures, containing seven introns in their coding regions and sharing 94% similarity of their deduced amino acid sequences with 38 substitutions. transcripts of both isoforms were detected throughout the embryogenesis, at low ...201525668703
display of isknv orf086 protein on the surface of aeromonas hydrophila and its immunogenicity in chinese perch (siniperca chuatsi).co-infection with infectious spleen and kidney necrosis virus (isknv) and aeromonas hydrophila is becoming ever more widespread in chinese perch (siniperca chuatsi) aquaculture industry, so that it's necessary to develop the combined vaccine against isknv and a. hydrophila disease. the surface display of heterologous on bacteria using anchoring motifs from outer membranes proteins has already been explored as an effective delivery system of viral antigens. in present study, the isknv orf086 gene ...201627436517
early protein orf086 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in mandarin fish siniperca chuatsi.infectious spleen and kidney necrosis virus (isknv) has caused significant loss in the mandarin fish (siniperca chuatsi) aquaculture industry. vaccination is an important measure to prevent fatal isknv infection. in this study, the orf086 gene encoding an early protein helicase of isknv was cloned into the prokaryotic pet32a (+) and eukaryotic pcdna3.1 (+) expression vectors and designated as pet086 and pcdna086, respectively. a recombinant 36 kda protein was detected in escherichia coli bl21 (h ...201526099219
genetic diversity of perch rhabdoviruses isolates based on the nucleoprotein and glycoprotein genes.despite the increasing impact of rhabdoviruses in european percid farming, the diversity of the viral populations is still poorly investigated. to address this issue, we sequenced the partial nucleoprotein (n) and complete glycoprotein (g) genes of nine rhabdoviruses isolated from perch (perca fluviatilis) between 1999 and 2010, mostly from france, and analyzed six of them by immunofluorescence antibody test (ifat). using two rabbit antisera raised against either the reference perch rhabdovirus ...201121927897
[Polymorphism of SNPs in the lipoprotein lipase (LPL) in Siniperca chuatsi and their association with feed habit domestication].Sinperca chuatsi usually refuses eating dead bait or man-made feed due to its specific feeding habit. It was showed that some S. chuatsi could be induced to take lifeless bait with domestication gradually after long-term of cultivation. High cost, serious pollution, severe diseases, and other problems of S chuatsi cultivation can be solved effectively by selecting those liable to domestication via molecular marker and treating them with artificial feed on a large scale. Lipoprotein lipase, one o ...201121951801
risk assessment for human consumption of perfluorinated compound-contaminated freshwater and marine fish from hong kong and xiamen.perfluorinated compounds (pfcs) are man-made fluoro-surfactants that are identified as global pollutants and can pose health risks to humans and wildlife. two aspects of risk assessment were conducted in this study, including exposure and response. exposure was estimated by using the concentrations of pfcs in fish and applying standard exposure factors. among different pfcs, pfos, pfoa, pfna, pfda, pfuda and pftrda were detected. total concentrations of pfc in fish ranged from 0.27-8.4 ng g(-1) ...201121705041
complementary dna sequences of the constant regions of t-cell antigen receptors α, β and γ in mandarin fish, siniperca chuatsi basilewsky, and their transcriptional changes after stimulation with flavobacterium columnare.in this study, the constant-region genes (cα, cβ and cγ) that encode the t-cell antigen receptor (tcr) α, β and γ chains were cloned from mandarin fish, siniperca chuatsi basilewsky, an important freshwater fish species in china. the complementary dna sequences of cα, cβ and cγ were 843, 716 and 906 base pairs (bp) in length and had a 465-, 289- and 360-bp 3' untranslated region, encoding 125, 142 and 182 amino acids, respectively. the amino-acid sequences of the constant regions of mandarin fis ...201424330001
application and development of a taqman real-time pcr for detecting infectious spleen and kidney necrosis virus in siniperca chuatsi.infectious spleen and kidney necrosis virus (isknv) is one of the major epidemiological agents that had caused great economic loss in chinese perch (siniperca chuatsi). in this study, a specific taqman real-time pcr was developed using a pair of primers and a taqman probe specific to the orf007 gene of isknv to rapidly detect isknv copies in chinese perch samples. this assay was optimized to produce linearity from 8.75 × 10(8) to 8.75 × 10(1) copies in standard curve with an efficiency of 98% an ...201728323153
assessment of risk of pcdd/fs and dioxin-like pcbs in marine and freshwater fish in pearl river delta, china.fish consumption is known to be beneficial to human health. however since the age of industrialization, the released/disposed chemical pollutants into water systems make fish a source of various environmental toxicants to humans. in oceanic cities with heavy industrial activities, fish products contribute the greatest proportion of exposure to pollutants. in this study, risks and potential effects of dioxins to health of coastal populations in the pearl river delta were assessed. concentrations ...201121392810
transcriptomic analysis of mandarin fish brain cells infected with infectious spleen and kidney necrosis virus with an emphasis on retinoic acid-inducible gene 1-like receptors and apoptosis pathways.infectious spleen and kidney necrosis virus (isknv) has caused significant economic losses in the cultured mandarin fish (siniperca chuatsi) industry. the molecular mechanisms that underlie the pathogenesis of the viral infection remain poorly understood. in this study, deep rna sequencing technique was used to analyze the transcriptomic profiles of mandarin fish brain cells (cpb) at progressive time points after isknv infection. a total of 96,206,040 clean data from 98,235,240 sequence reads we ...201525982401
a novel fish cell line derived from the brain of chinese perch siniperca chuatsi: development and characterization.in this study, a continuous cell line (named as cpb) was established from siniperca chuatsi brain and has been subcultured >140 times. cpb cell line predominantly consisted of fibroblast-like cells that could grow better in leibovitz's l-15 supplemented with 10% foetal bovine serum at 28° c. polymerase chain reaction amplification of 18s recombinant (r)rna confirmed the origin of this cell line from s. chuatsi. the cpb cell line was cryopreserved at different passage levels and revived successfu ...201525376532
interaction of infectious spleen and kidney necrosis virus orf119l with pinch leads to dominant-negative inhibition of integrin-linked kinase and cardiovascular defects in zebrafish.infectious spleen and kidney necrosis virus (isknv) is the type species of the megalocytivirus genus, iridoviridae family, causing a severe systemic disease with high mortality in mandarin fish (siniperca chuatsi) in china and southeast asia. at present, the pathogenesis of isknv infection is still not fully understood. based on a genome-wide bioinformatics analysis of isknv-encoded proteins, we found that isknv open reading frame 119l (orf119l) is predicted to encode a three-ankyrin-repeat (3an ...201525355883
cloning, characterization, and biological function analysis of the sidt2 gene from siniperca chuatsi.the systemic rna interference defective protein (sid)-1 plays an important role in dsrna uptake in cells. we identified the scsidt2 gene from the mandarin fish (siniperca chuatsi), which is the first-studied sid-1 homolog in fish. scsidt2 mrna is 3380 bp long and contains a 2568-bp open reading frame that encodes an 855-amino-acid protein with an n-terminal signal peptide and ten putative transmembrane domains. tissue distribution profile in healthy fish and expression profiles of scsidt2 in inf ...201121334374
mercury species of sediment and fish in freshwater fish ponds around the pearl river delta, pr china: human health risk assessment.this study investigated total mercury (thg) and methylmercury (mehg) concentrations in five species of freshwater fish and their associated fish pond sediments collected from 18 freshwater fish ponds around the pearl river delta (prd). the concentrations of thg and mehg in fish pond surface sediments were 33.1-386 ng g(-1) dry wt and 0.18-1.25 ng g(-1) dry wt, respectively. the age of ponds affected the surface sediment mehg concentration. the vertical distribution of mehg in sediment cores show ...201121272914
genotype and host range analysis of infectious spleen and kidney necrosis virus (isknv).infectious spleen and kidney necrosis virus (isknv) is the causative agent of a disease causing high mortality in mandarin fish, siniperca chuatsi. in this study, complete major capsid protein (mcp) genes of nine isknv isolates were sequenced and compared with other known megalocytiviruses to evaluate genetic variation and host range of the viruses. comparison of nucleotide sequences of mcp gene revealed 92.6-100% identity among nine isknv isolates. a phylogenetic tree revealed that 33 megalocyt ...201021107672
signal transducer and activator of transcription 3 (stat3) homologue in turbot (scophthalmus maximus): molecular characterization and expression analysis.signal transducer and activator of transcription 3 (stat3) acts as an important mediator in multiple biological processes induced by different cytokines. so far, little information is available in fish stat3. in this study, turbot (scophthalmus maximus) stat3 gene was cloned and characterized for the first time. the turbot stat3 full-length cdna consists of 2355 nucleotides encoding a polypeptide of 784 amino acids with four conserved domains including stat_int, stat_alpha, stat_bind and sh2 dom ...201021035551
a new marine megalocytivirus from spotted knifejaw, oplegnathus punctatus, and its pathogenicity to freshwater mandarinfish, siniperca chuatsi.megalocytivirus is a newly defined piscine iridovirus and has been shown to be an important causative agent of viral diseases in fish. here, a new megalocytivirus strain, designated skiv-zj07, was isolated from spotted knifejaw (oplegnathus punctatus) using a mandarinfish fry cell line (mff-1). phylogenetic analysis of the major capsid protein and atpase genes showed that skiv-zj07 was most similar to the orange-spotted grouper iridovirus (osgiv) from china and a u1 strain red sea bream iridovir ...201019895861
the potential role of microfilaments in host cells for infection with infectious spleen and kidney necrosis virus infection.infectious spleen and kidney necrosis virus (isknv) belongs to the genus megalocytivirus from the family iridoviridae. megalocytivirus causes severe economic losses to tropical freshwater and marine culture industry in asian countries and is devastating to the mandarin fish farm industry in china particularly.201323497248
mandarin fish caveolin 1 interaction with major capsid protein of infectious spleen and kidney necrosis virus and its role in early stages of infection.infectious spleen and kidney necrosis virus (isknv) is the type species of the genus megalocytivirus from the family iridoviridae. isknv is one of the major agents that cause mortality and economic losses to the freshwater fish culture industry in asian countries, particularly for mandarin fish (siniperca chuatsi). in the present study, we report that the interaction of mandarin fish caveolin 1 (mcav-1) with the isknv major capsid protein (mcp) was detected by using a virus overlay assay and con ...201323283951
the alpha inhibitor of nf-kappab (ikappabalpha) from the mandarin fish binds with p65 nf-kappab.the nf-kappab/ikappabalpha pathway plays an important role in the regulation of immune and inflammatory responses. ikappabalpha is an inhibitory molecule that sequesters transcription activator nf-kappab dimer in the cytoplasm of unstimulated cells. here, we isolated the full-length cdnas of the mandarin fish (siniperca chuatsi) alpha inhibitor of nf-kappab (scikappabalpha) and p65 nf-kappab (scp65). multiple sequence alignments showed that the amino acid sequences of both scikappabalpha and scp ...200919340946
protective immunity against iridovirus disease in mandarin fish, induced by recombinant major capsid protein of infectious spleen and kidney necrosis virus.infectious spleen and kidney necrosis virus (isknv) is the causative agent of a disease causing high mortality and economic losses in mandarin fish, siniperca chuatsi in china. but little information about vaccine development against isknv disease is available. in this study the gene encoding the major capsid protein (mcp), which is predominant structural component of the iridovirus particles, was cloned into a temperature induction prokaryotic expression vector pbv220 and a recombinant protein ...201222971336
the viral traf protein (orf111l) from infectious spleen and kidney necrosis virus interacts with tradd and induces caspase 8-mediated apoptosis.infectious spleen and kidney necrosis virus (isknv) is the type species of the megalocytivirus genus of the iridoviridae family. it causes a serious and potentially pandemic disease in wild and cultured fishes. isknv infection induces evident apoptosis in mandarin fish (siniperca chuatsi) and zebrafish (danio renio). however, the mechanism is still unknown. after a genome-wide bioinformatics analysis of isknv-encoded proteins, the isknv open reading frame 111l (orf111l) shows a high similarity t ...201222615868
molecular cloning and characterization of a new g-type lysozyme gene (ec-lysg) in orange-spotted grouper, epinephelus coioides.lysozyme acts as an innate immunity molecule against pathogen infection. in this study, a new g-type lysozyme gene with a typical g-type lysozyme domain (designated as ec-lysg) was cloned and characterized from the orange-spotted grouper, epinephelus coioides. the full-length ec-lysg cdna contains 1419 bp and encodes a 256-residue protein containing a 25-residue signal peptide at the n-terminus. blast analysis reveals ec-lysg shares 64% identity with siniperca chuatsi, but 63% to another reporte ...201424877656
identification, molecular cloning of il-1β and its expression profile during nocardia seriolae infection in largemouth bass, micropterus salmoides.in the present study, il-1β cdna was identified and analyzed from largemouth bass (micropterus salmoides). full length il-1β mrna was obtained using rapid amplification of cdna ends (race), which contains 78 bp 3'-utr, a 455 bp 5'-utr, and an open reading frame (orf) of 702 bp coding for 233 amino acid residues. the molecular weight and theoretical isoelectric point of largemouth bass il-1β protein was predicted to be 26.7 kda and 6.08 respectively. a largemouth bass il-1β phylogenetic analysis ...201627706080
cloning of irak1 and its upregulation in symptomatic mandarin fish infected with isknv.interleukin-1 receptor activated kinases (iraks) play crucial roles in the toll-like receptor (tlr) mediated signal transduction pathways that control host innate immune responses. here we report the cloning of an irak1 cdna (named scirak1) from the mandarin fish. the predicted scirak1 peptide contains a death domain and a serine/threonine-specific kinase domain. quantitative rt-pcr showed that scirak1 mrna was primarily expressed in blood cells and posterior kidney. seven days following infecti ...200919336221
cloning, characterization and expression analysis of a cxcr1-like gene from mandarin fish siniperca chuatsi.in this study we cloned and characterized a cxcr1-like gene (mfcxcr1) from mandarin fish (siniperca chuatsi). the full-length cdna of mfcxcr1 is 2,173 bp and contains a 1,056 bp open reading frame (orf) that encodes a protein of 351 amino acids. the 5' and 3' untranslated regions (utr) are 57 and 1,080 bp in length, respectively. the coding region of the mfcxcr1 gene consists of a single exon with a 734 bp intron that is two nucleotides upstream of the atg start codon in the 5' utr. the mfcxcr1 ...200919093223
emergence of a new rhabdovirus associated with mass mortalities in eelpout (zoarces viviparous) in the baltic sea.we report the first description of a new rhabdoviridae tentatively named eelpout rhabdovirus (eprv genus perhabdovirus). this virus was associated with mass mortalities in eelpout (zoarces viviparous, linnaeus) along the swedish baltic sea coast line in 2014. diseased fish showed signs of central nervous system infection, and brain lesions were confirmed by histology. a cytopathogenic effect was observed in cell culture, but elisas for the epizootic piscine viral haemorrhagic septicaemia virus ( ...201727416895
molecular cloning and expression analysis of igd in nile tilapia (oreochromis niloticus) in response to streptococcus agalactiae stimulus.igd is considered to be a recently-evolved ig and a puzzling molecule, being previously found in all vertebrate taxa, except for birds. although igd likely plays an important role in vertebrate immune responses, the function of igd in nile tilapia (oreochromis niloticus) is virtually unknown. in the present study, a membrane form of igd (migd) heavy chains were cloned from the gift strain of nile tilapia (designated on-migd). the on-migd heavy chain's cdna is composed of 3347 bp with a 31 bp of ...201627005611
mitochondrial diversity and phylogeography of the chinese perch, siniperca chuatsi (perciformes: sinipercidae). 200818602842
metallothionein-2 gene from the mandarin fish siniperca chuatsi: cdna cloning, tissue expression, and immunohistochemical localization.the metallothionein-2 (mt-2) gene was isolated from the mandarin fish, one of the most important industrial aquatic animals in china, by using rapid amplification of cdna ends (race). the deduced amino acid sequence of mt-2 comprised 60 amino acids and showed approximately 62.3% identity to human metallothionein. its promoter region was amplified by thermal asymmetric interlaced polymerase chain reaction (tail-pcr). the mt-2 gene consists of 3 exons and 2 introns, extending approximately 900 bp ...200918582600
outbreaks and risks of infectious spleen and kidney necrosis virus disease in freshwater ornamental fishes.we examined the distribution of iridoviruses in 10 freshwater ornamental fish species hatched in korea and imported from other asian countries using both 1-step and 2-step polymerase chain reation (pcr). none of the 10 fish species analyzed were free of iridovirus as shown by 2-step pcr positive results, and 3 species yielded 1-step pcr positive results with associated mortality. cloned pcr amplicons of the adenosine triphosphatase (atpase) and major capsid protein (mcp) genes in genomic dna of ...200818380219
flow cytometric determination of genome size for eight commercially important fish species in china.the genome size (c value) of eight commercially important fish species in china was measured using flow cytometry. chicken (gallus domesticus) erythrocytes were used as reference cells. when using propidium iodide (pi) as the fluorescent dye, genome sizes were 1.09 ± 0.08, 2.75 ± 0.12, 1.05 ± 0.05, 1.35 ± 0.11, 0.99 ± 0.05, 0.90 ± 0.08, 0.90 ± 0.07, and 0.88 ± 0.07 pg for japanese eel (anguilla japonica), mullet (myxocyprinus asiaticus), yellowcheek carp (elopichthys bambusa), blunt snout bream ...201222956044
trail in the mandarin fish siniperca chuatsi: gene and its apoptotic effect in hela cells.tumor necrosis factor (tnf)-related apoptosis-inducing ligand (trail) is one of the tnf superfamily members, participating in many biological processes including cell proliferation and apoptotic death. in this study, a trail gene was cloned from a perciform fish, the mandarin fish siniperca chuatsi, a major cultured fish in china's aquaculture, and is named as sctrail for s. chuatsi trail. the full-length cdna of sctrail is 1359bp, encoding a 283-amino-acid protein. this deduced protein contains ...200818083044
monitoring and assessment of heavy metal contamination in a constructed wetland in shaoguan (guangdong province, china): bioaccumulation of pb, zn, cu and cd in aquatic and terrestrial components.the objective of this study is to evaluate the current status of heavy metal concentrations in constructed wetland, shaoguan (guangdong, china). sediments, three wetland plants (typha latifolia, phragmites australis, and cyperus malaccensis), and six freshwater fish species [carassius auratus (goldfish), cirrhinus molitorella (mud carp), ctenopharyngodon idellus (grass carp), cyprinus carpio (wild common carp), nicholsicypris normalis (mandarin fish), sarcocheilichthys kiangsiensis (minnows)] in ...201727164879
tumor necrosis factor-alpha gene from mandarin fish, siniperca chuatsi: molecular cloning, cytotoxicity analysis and expression profile.in this report, we cloned a cdna and the gene of mandarin fish (siniperca chuatsi) tumor necrosis factor-alpha (tnf-alpha). the length of tnf-alpha cdna was 1461 bp, contained an open reading frame (orf) of 762 bp, which encoded 253 amino acids. the positions of cysteine residues, transmembrane sequence, and protease cleavage site were similar with other reported fish tnf-alpha and mammalian tnf-alpha. mandarin fish tnf-alpha gene has a length of 1940 bp, and consists of four exons and three int ...200717482265
arsenic contamination in the freshwater fish ponds of pearl river delta: bioaccumulation and health risk assessment.this study investigated the extent of arsenic (as) contamination in five common species of freshwater fish (northern snakehead [channa argus], mandrarin fish [siniperca chuatsi], largemouth bass [lepomis macrochirous], bighead carp [aristichthys nobilis] and grass carp [ctenopharyngodon idellus]) and their associated fish pond sediments collected from 18 freshwater fish ponds around the pearl river delta (prd). the total as concentrations detected in fish muscle and sediment in freshwater ponds ...201323247527
differential gene expression profile in spleen of mandarin fish siniperca chuatsi infected with isknv, derived from suppression subtractive hybridization.to study the interaction between an invading virus and its host, we investigated differential gene expression in mandarin fish siniperca chuatsi experimentally infected with infectious spleen and kidney necrosis virus (isknv). subtractive cdna libraries were constructed by suppression subtractive hybridization (ssh) from spleens of mock- and isknv-infected fish. both forward- and reverse-subtracted libraries were generated. in the forward library, genes of the ubiquitin-proteasome proteolytic pa ...200617260830
redescription of trypanosoma siniperca chang 1964 from freshwater fish of china based on morphological and molecular data.during the parasite fauna investigation within 2005, the freshwater fish trypanosome trypanosoma siniperca chang 1964 was isolated from the blood of mandarin carp (siniperca chuatsi) from niushan lake, hubei province, central china. blood trypomastigotes were observed only, and the density of infection was low. light microscopy examinations of this material made it possible to study in detail the morphology of this parasite and redescribe it according to current standards. t. siniperca is charac ...200717063366
identification, localization and morphology of apud cells in gastroenteropancreatic system of stomach-containing teleosts.aim:to identify the type localization and morphology of apud endocrine cells in the gastroenteropancreatic (gep) system of stomach-containing teleosts, and study apud endocrine system in the stomach, intestine and pancreas of fish species.methods:two kinds of immunocytochemical (icc) techniques of the streptavidin biotin-peroxidase complex (sabc) and streptavidin-peroxidase (s-p) method were used. the identification, localization and morphology of apud endocrine cells scattered in the mucosa of ...200011819706
genomic structure and immunological response of an stat4 family member from rock bream (oplegnathus fasciatus).the janus tyrosine kinase (jak)/signal transducer and activator of transcription (stat) signaling pathway plays a critical role in host defense against viral and bacterial infections. stat proteins are a group of transcription factors that translocate into the nucleus and are critical for the induction of many genes crucial for the allergic cascade and immune defense. in the present study, a member of the stat4 family was identified from rock bream (rbstat4) at the genomic level, and its transcr ...201324055510
molecular characterization and expression pattern of tumor suppressor protein p53 in mandarin fish, siniperca chuatsi following virus challenge.in recent years, the tumor suppressor protein p53, which is crucial for cellular defense against tumor development, has also been implicated in host antiviral defense. in the present study, a 1555 bp full-length cdna of p53 from mandarin fish (siniperca chuatsi) (sc-p53) was cloned and characterized. quantitative real-time pcr assays revealed that sc-p53 was expressed in all tissues examined, and it was most abundant in the gill and kidney. recombinant sc-p53 fused with a his·tag was expressed i ...201626980610
phylogenetic studies of sinipercid fish (perciformes: sinipercidae) based on multiple genes, with first application of an immune-related gene, the virus-induced protein (viperin) gene.the sinipercid fish represent a group of 12 species of freshwater percoid fish endemic to east asia. to date published morphological and molecular phylogenetics hypotheses of sinipercid fish are part congruent, and there are some areas of significant disagreement with respect to species relationships. the present study used separate and combined methods to analyze 7307 bp of data from three mitochondrial genes (cyt b, co1 and 16s rrna; approximately 2312 bp) and three nuclear genes (viperin, the ...201020138219
structure of the mitochondrial dna control region of the sinipercine fishes and their phylogenetic relationship.the mitochondrial dna control region of siniperca chuatsi, s. kneri, s. scherzeri, s. obscura, s. undulata, coreosiniperca roulei and coreoperca whiteheadi were amplified by pcr amplification and directly sequenced. the mtdna control region of the sinipercine fishes could be separated into three domains, namely, the terminal associated sequence domain, the central conserved sequence domain and the conserved sequence block domain. the extended terminal associated sequence (etas), three conserved ...200616980125
maintaining economic value of ecosystem services whilst reducing environmental cost: a way to achieve freshwater restoration in china.freshwater fisheries are central to food security in china and this remains one of the most important priorities for the growing human population. thus, combining ecosystem restoration with economics is pivotal in setting successful conservation in china. here, we have developed a practical management model that combines fishery improvement with conservation. for six years, a ban on fertilizer and a reduction of planktivorous fish stocking along with the introduction of both mandarin fish sinipe ...201525803696
purification and characterisation of trypsins from the pyloric caeca of mandarin fish (siniperca chuatsi).two trypsins of anionic form (trypsin a) and cationic form (trypsin b) from the pyloric caeca of mandarin fish (siniperca chuatsi) were highly purified by a series of chromatographies, including deae-sephacel, sephacryl s-200 hr, q-sepharose or sp-sepharose. purified trypsins revealed a single band on native-page. the molecular weights of trypsin a and b were 21kda and 21.5kda, respectively, as estimated by sds-page, both under reducing and non-reducing conditions. zymography analysis showed tha ...200826049226
cloning, molecular characterization, and expression analysis of the signal transducer and activator of transcription 3 (stat₃) gene from grass carp (ctenopharyngodon idellus).signal transducer and activator of transcription 3 (stat₃) binds to janus kinase 2 (jak₂) to initiate the jak₂/stat₃ signal transduction pathway, which plays an important role in cancer cell proliferation, immune regulation, reproduction, lipid metabolism, and other physiological processes of the organism. in this study, the cdna sequence of the stat₃ gene from grass carp was cloned using race (rapid-amplification of cdna ends). twelve characteristics of the stat₃ gene and its encoded protein se ...201324055509
protein encoded by orf093 is an effective vaccine candidate for infectious spleen and kidney necrosis virus in chinese perch siniperca chuatsi.infectious spleen and kidney necrosis virus (isknv) is the causative agent of a disease causing high mortality and economic losses in chinese perch, siniperca chuatsi in china. little information about the antigenicity of isknv proteins is available. in this study the orf093 gene of isknv was cloned into the prokaryotic expression vector pet32a(+) and eukaryotic expression vector pcdna3.1(+), and designated as pet-093 and pcdna-093, respectively. a recombinant 51-kda protein was detected in esch ...201525462463
assessment of heavy metals/metalloid (as, pb, cd, ni, zn, cr, cu, mn) concentrations in edible fish species tissue in the pearl river delta (prd), china.the major aim of this study was to investigate heavy metal content of edible fish in the prd. eleven species of fish (consisting of 711 individuals) [catfish (clarias fuscus), tilapia (oreochromis mossambicus), grass carp (ctenopharyngodon idellus), bighead carp (aristichthys nobilis), mandarin fish (siniperca kneri), snakehead (channa asiatiea), black bass (micropterus salmoides), mangrove snapper (lutjanus griseus), star snapper (lutjanu stellatus), snubnose pompano (trachinotus blochii) and o ...201424239097
molecular cloning, expression and functional characterization of interferon-γ-inducible lysosomal thiol reductase (gilt) gene from mandarin fish (siniperca chuatsi).interferon-γ-inducible lysosomal thiol reductase (gilt) plays a key role in the processing and presentation of mhc class ii-restricted antigen (ag) by catalyzing disulfide bond reduction, thus unfolding native protein ag and facilitating subsequent cleavage by proteases. for this important function in the immune system, we cloned a gilt gene homologue from mandarin fish (designated mgilt), a kind of precious freshwater fish with high market value. through reverse transcription pcr and rapid ampl ...201424698993
analysis of the transcriptomic profilings of mandarin fish (siniperca chuatsi) infected with flavobacterium columnare with an emphasis on immune responses.flavobacterium columnare (fc) is the causative pathogen of columnaris which has caused great economic loss in fish culture worldwide, including in mandarin fish (siniperca chuatsi) culture. in the present study, the transcriptomic profiles of the head kidneys from fc-infected and non-infected mandarin fish were obtained using hiseq™ 2000 (illumina). totally 31,168 unigenes with high quality were obtained. genes involved in protein folding, metabolism and energy, immune responses, oxidoreductase ...201525533996
glutamine and glutaminolysis are required for efficient replication of infectious spleen and kidney necrosis virus in chinese perch brain cells.viruses rely on host cellular metabolism for energy and macromolecule synthesis during their replication. infectious spleen and kidney necrosis virus (isknv) causes significant economic losses in the chinese perch (siniperca chuatsi) industry worldwide. however, little is known about the relationship between isknv replication and cellular metabolism. using transcriptomic analysis, we observed that glutamine metabolism in chinese perch brain (cpb) cells is altered during isknv infection. moreover ...201727911855
protective immunity against infectious spleen and kidney necrosis virus induced by immunization with dna plasmid containing mcp gene in chinese perch siniperca chuatsi.infectious spleen and kidney necrosis virus (isknv) is the causative agent of a disease leading to high mortality and economic losses in chinese perch, siniperca chuatsi. there is an urgent need to develop an effective vaccine against this fatal disease. in this study, the mcp gene encoding the major capsid protein, the predominant structural component of the iridovirus particles, was cloned into a eukaryotic expression vector pcdna3.1+, and the recombinant plasmid, designated as pcmcp, was cons ...201425038286
orf005l from infectious spleen and kidney necrosis virus is located in the inner mitochondrial membrane and induces apoptosis.infectious spleen and kidney necrosis virus (isknv) belongs to the genus megalocytivirus in the family iridoviridae. this virus is the etiological agent of a serious and pandemic disease in fish. cells infected with isknv undergo apoptosis. in this study, the isknv orf005l gene was characterized and functionally investigated. bioinformatics analysis revealed that the orf005l protein contains a region similar to the catalytic domain of ctd-like phosphatases. real-time quantitative-pcr results sho ...201424862228
disease resistance and immune-relevant gene expression in golden mandarin fish, siniperca scherzeri steindachner, infected with infectious spleen and kidney necrosis virus-like agent.infectious spleen and kidney necrosis virus (isknv), family iridoviridae, genus megalocytivirus, may cause high mortality rates such as those seen in mandarin fish, siniperca chuatsi. isknv has attracted much attention due to the possible environmental threat and economic losses it poses on both cultured and wild populations. we have investigated the pathogenicity of isknv-like agent megalocytivirus, isolated from infected pearl gourami, in golden mandarin fish, siniperca scherzeri - a member of ...201424111797
field trial tests of fkc vaccines against rsiv genotype megalocytivirus in cage-cultured mandarin fish (siniperca chuatsi) in an inland reservoir.megalocytiviruses are one of the most important causative agents in finfish industry in china, japan and south east asia. the viruses are mainly composed of isknv, rsiv and trbiv genotypes. among them, isknv genotype isolate is the most important causative agent in mandarin fish industry in south china. since its first occurrence in mid-1990s in china, no effective drug has been developed to prevent and control this virus until our recent work. in this study, unusual rsiv genotype megalocytiviru ...201324035751
the first case of infectious spleen and kidney necrosis virus (isknv) infection in aquarium-maintained mandarin fish, siniperca chuatsi (basilewsky), in japan. 201423763499
efficacy of a formalin-killed cell vaccine against infectious spleen and kidney necrosis virus (isknv) and immunoproteomic analysis of its major immunogenic proteins.infectious spleen and kidney necrosis virus (isknv), the type species of genus megalocytivirus in the family iridoviridae, is the most important etiological agents in mandarin fish industry in china. since its first occurrence in china in the early 1990s, there is no effective method to prevent and control this virus. here we report the successful development of a formalin-killed cell-cultured (fkc) vaccine again isknv. immuno-protection experiments showed that greater than 90% of fish immunized ...201323177910
susceptibility of chinese perch brain (cpb) cell and mandarin fish to red-spotted grouper nervous necrosis virus (rgnnv) infection.nervous necrosis virus (nnv) is the causative agent of viral encephalopathy and retinopathy (ver), a neurological disease responsible for high mortality of fish species worldwide. taking advantage of our established chinese perch brain (cpb) cell line derived from brain tissues of mandarin fish (siniperca chuatsi), the susceptibility of cpb cell to red-spotted grouper nervous necrosis virus (rgnnv) was evaluated. the results showed that rgnnv replicated well in cpb cells, resulting in cellular a ...201627213348
identification of a myeloperoxidase-like ortholog from rock bream (oplegnathus fasciatus), deciphering its transcriptional responses to induced pathogen stress.myeloperoxidases (mpos) are heme-linked oxidative stress-generating enzymes found abundantly in azurophilic granules of polymorphonuclear neutrophils. mature mpos act as potent antimicrobial agents by producing hypohalous acids using hydrogen peroxide and halide ions as substrates. these acids can readily oxidize reactive groups of biomolecules on invading microbes. in this study, we identified and characterized a homolog of mpo from rock bream (oplegnathus fasciatus), designated as rbmpo. we an ...201525982406
metal concentrations in various fish organs of different fish species from poyang lake, china.concentrations of as, cd, cr, cu, hg, ni, pb, and zn in the muscle of eleven fish species (bighead carp, bream, catfish, carp, crucian, culter alburnus, grass carp, mandarin fish, white semiknife carp, silver carp, and yellow catfish) from poyang lake were analysed using inductive coupling plasma mass spectrometry. metal levels in other organs (e.g., bladder, gill, kidney, liver, and spleen) of bighead carp, carp, grass carp, and silver carp were also determined. the results showed that metal co ...201424681447
characterization of cdna encoding immunoglobulin light chain of the mandarin fish (siniperca chuatsi).immunoglobulin light chain cdna sequences of a perciform fish, the mandarin fish siniperca chuatsi were amplified from head kidney mrna by reverse transcription (rt)-pcr and race methods using degenerated primer and gene specific ones. in cdna sequences of the vl region, nucleotide exchanges were present mainly within cdrs, although a lesser degree of variability was also found in frs. moreover, the length of cdr1 and cdr3 in the mandarin fish is shorter than in most other fish species. in the m ...200312969639
perfluorinated alkyl substances in water, sediment, plankton and fish from korean rivers and lakes: a nationwide survey.water, sediment, plankton, and blood and liver tissues of crucian carp (carassius auratus) and mandarin fish (siniperca scherzeri) were collected from six major rivers and lakes in south korea (including namhan river, bukhan river, nakdong river, nam river, yeongsan river and sangsa lake) and analyzed for perfluorinated alkyl substances (pfass). perfluorooctane sulfonate (pfos) was consistently detected at the greatest concentrations in all media surveyed with the maximum concentration in water ...201424529450
the viral ankyrin repeat protein (orf124l) from infectious spleen and kidney necrosis virus attenuates nf-{kappa}b activation and interacts with ikk{beta}the ankyrin (ank) repeat is one of the most common protein-protein interaction motifs predominantly found in eukaryotes and bacteria, but the functions of ank repeat are rarely researched in animal viruses with the exception of poxviruses. infectious spleen and kidney necrosis virus (isknv) is a typical species of the genus megalocytivirus in the family iridoviridae and a causative agent of epizootics in fish. the genome of isknv contains four putative viral ankyrin (vank) repeat proteins, and t ...201121471317
isolation and characterization of 46 novel polymorphic est-simple sequence repeats (ssr) markers in two sinipercine fishes (siniperca) and cross-species amplification.with the development of next generation sequencing technologies, transcriptome level sequence collections are emerging as prominent resources for the discovery of gene-based molecular markers. in this study, we described the isolation and characterization of 46 novel polymorphic microsatellite loci for siniperca chuatsi and siniperca scherzeri from the transcriptome of their f(1) interspecies hybrids. forty-three of these loci were polymorphic in s. chuatsi, and 20 were polymorphic in s. scherze ...201222949814
autophagy promoted infectious kidney and spleen necrosis virus replication and decreased infectious virus yields in cpb cell line.autophagy plays important functions in viral replication and pathogenesis. in this study, we investigated the role of autophagy in the replication of infectious kidney and spleen necrosis virus (isknv), an agent that has caused devastating losses in chinese perch (siniperca chuatsi) industry. we found that isknv infection triggered the complete autophagic process, as demonstrated by microtubule-associated protein 1 light chain 3b ii (lc3b-ii) conversion, an increased accumulation of punctate gfp ...201727856327
cloning of a new fibroblast cell line from an early primary culture from mandarin fish (siniperca chuatsi) fry for efficient proliferation of megalocytiviruses.megalocytiviruses are important emerging pathogens in both freshwater and marine finfish aquaculture. however, a limited number of piscine cell lines are persistently susceptible to these viruses, which greatly limits the study of megalocytiviruses. in this study, a new fibroblast-like cell line was established from an early primary culture from mandarin fish fry by a single cell cloning and was designated as mff-8c1. the mff-8c1 cells grow well in dulbecco's modified eagle's medium supplemented ...201424101440
resting metabolism and heat increment of feeding in mandarin fish (siniperca chuatsi) and chinese snakehead (channa argus).resting metabolism was measured in immature mandarin fish siniperca chuatsi weighing 42.1-510.2 g and chinese snakehead channa argus weighing 41.5-510.3 g at 10, 15, 20, 25, 30 and 35 degrees c. heat increment of feeding was measured in mandarin fish weighing 202.0 (+/-14.0) g and snakehead weighing 200.8 (+/-19.3) g fed swamp loach misgurnus anguillicaudatus at 1% body weight per day at 28 degrees c. in both species, weight exponent in the power relationship between resting metabolism and body ...200011064280
spatiotemporal distribution and assemblages of fishes below the lowermost dam in protected reach in the yangtze river main stream: implications for river management.now more and more ecologists concern about the impacts of dam construction on fish. however, studies of fishes downstream gezhouba dam were rarely reported except chinese sturgeon (acipenser sinensis gray). in this study, catch investigations and five hydroacoustic detections were completed from 2015 to 2016 to understand the distribution, size, and categories of fishes and their relationship with the environmental factors below gezhouba dam in protected reach in the yangtze river main stream. r ...201627843943
isolation and characterization of novel genomic and est-ssr markers in coreoperca whiteheadi boulenger and cross-species amplification.we described and characterized 11 expressed sequence tag (est)-derived simple sequence repeats (ssr) and seven genomic (g)-derived ssrs in coreoperca whiteheadi boulenger. the est-ssrs comprised 62.2% di-nucleotide repeats, 32.2% tri-nucleotide repeats and 5.5% tetra-nucleotide repeats, whereas the majority of the g-ssrs were tri-nuleotide repeats (81.4%). the number of alleles for the 18 loci ranged from 3 to 6, with a mean of 3.8 alleles per locus. the observed (ho) and expected heterozygositi ...201223202947
[cloning and expression analysis of the nfκb inhibitor iκbα of ayu (plecoglossus altivelis)].the nfκb inhibitor (iκbα) is an integral part of nfκb/iκb signaling pathways, which plays roles in a variety of immune responses, such as bacterial infection resistance. by interacting with nuclear transcription factor nfκb, iκbα controls a variety of biological immune gene expressions. in this study, full-length cdna (1341 bp) of the nfκb inhibitor iκbα (paiκbα, genbank accession no. jn801027) of plecoglossus altivelis was obtained by race and pcr, and included a 5' untranslated region (utr) (6 ...201323913891
molecular characterization of myf5 and comparative expression patterns of myogenic regulatory factors in siniperca chuatsi.myogenic regulatory factors (mrfs) are muscle-specific basic helix-loop-helix (bhlh) transcription factor that plays an essential role in regulating skeletal muscle development and growth. to investigate molecular characterization of myf5 and compare the expressional patterns of the four mrfs, we cloned the myf5 cdna sequence and analyzed the mrfs expressional patterns using quantitative real-time polymerase chain reaction in chinese perch (siniperca chuatsi). sequence analysis indicated that ch ...201626547039
evolution of type ii antifreeze protein genes in teleost fish: a complex scenario involving lateral gene transfers and episodic directional selection.i examined hypotheses about lateral transfer of type ii antifreeze protein (afp) genes among "distantly" related teleost fish. the effects of episodic directional selection on amino acid evolution were also investigated. the strict consensus results showed that the type ii afp and type ii antifreeze-like protein genes were transferred from osmerus mordax to clupea harengus, from the ancestral lineage of the brachyopsis rostratus-hemitripterus americanus clade to the ancestor of the hypomesus nip ...201223032610
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