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isolation and studies of the mutagenic activity in the ames test of flavonoids naturally occurring in medical herbs.quercetin, rhamnetin, isohamnetin, apigenin and luteolin were isolated from medicinal herbs: erigeron canadensis l., anthyllis vulneraria l. and pyrola chloranta l. the mutagenicity of these naturally occurring flavonoids was tested by the ames method with s. typhimurium strains ta1535, ta1538, ta97, ta98, ta100 and ta102 in the presence and absence of metabolic activation. of the above flavonoids only quercetin and rhamnetin revealed mutagenic activity in the ames test. quercetin induced point ...19902179716
[inhibitory effects of some crude and semi-purified extracts of indigenous french plants on the multiplication of human herpesvirus 1 and poliovirus 2 in cell culture].within a valorization program of natural regional resources, 50 ethanolic extracts of 41 indigenous plants have been subjected to chemical tests and antiviral screening. four plants: bryonia dioìca, anthyllis vulneraria, matricaria chamomilla, and m. inodora inhibit the growth of poliovirus. furthermore, three (a. vulneraria, m. chamomilla, and m. inodora) have an antiherpetic effect.19836317803
population dynamics along a primary succession gradient: do alpine species fit into demographic succession theory?understanding processes and mechanisms governing changes in plant species along primary successions has been of major importance in ecology. however, to date hardly any studies have focused on the complete life cycle of species along a successional gradient, comparing pioneer, early and late-successional species. in this study it is hypothesized that pioneer species should initially have a population growth rate, lambda, greater than one with high fecundity rates, and declining growth rates when ...200919273477
mesorhizobium metallidurans sp. nov., a metal-resistant symbiont of anthyllis vulneraria growing on metallicolous soil in languedoc, france.a polyphasic taxonomic approach was used to characterize 31 rhizobial isolates obtained from anthyllis vulneraria, a metallicolous legume species, growing close to a zinc mine in the south of france (saint laurent le minier). comparative analysis of nearly full-length 16s rrna gene sequences showed that these gram-negative bacteria belonged to the genus mesorhizobium and that they were related most closely to mesorhizobium tianshanense ors 2640(t). the phylogenetic relationships of these isolate ...200919329619
genetic diversity of root nodule bacteria nodulating lotus corniculatus and anthyllis vulneraria in sweden.very little is known about the genetic diversity and phylogeny of rhizobia nodulating lotus species in northern temperate regions. we have therefore studied the genetic diversity among a total of 61 root nodule bacteria isolated from lotus corniculatus and anthyllis vulneraria from different geographic sites and habitats in sweden by restriction fragment length polymorphism (rflp) of the internal transcribed spacer between their 16s rrna and 23s rrna (igs) region. a high diversity consisting of ...201121497473
genome-wide transcriptional responses of two metal-tolerant symbiotic mesorhizobium isolates to zinc and cadmium exposure.mesorhizobium metallidurans stm 2683t and mesorhizobium sp. strain stm 4661 were isolated from nodules of the metallicolous legume anthyllis vulneraria from distant mining spoils. they tolerate unusually high zinc and cadmium concentrations as compared to other mesorhizobia. this work aims to study the gene expression profiles associated with zinc or cadmium exposure and to identify genes involved in metal tolerance in these two metallicolous mesorhizobium strains of interest for mine phytostabi ...201323631387
effects of agrobacterial oncogenes in kidney vetch (anthyllis vulneraria l.).kidney vetch seedlings were induced to form hairy roots by inoculating their mesocotyls with the wild-type strain 15834 of agrobacterium rhizogenes or with the a. tumefaciens strain c58c1 containing a binary vector system (the pria4b as a helper and the vector pcb1346 bearing a ptic58-derived isopentenyl transferase gene (ipt, cytokinin biosynthetic gene) under control of its native regulatory sequences). transgenic lines of three distinct phenotypes were selected: (i) typically, the pri15834-tr ...199224201440
rhizobium metallidurans sp. nov., a symbiotic heavy metal resistant bacterium isolated from the anthyllis vulneraria zn-hyperaccumulator.a gram-stain-negative, aerobic, rod-shaped, non-spore-forming bacterium (chimec512(t)) was isolated from 56 host seedlings of the hyperaccumulating anthyllis vulneraria legume, which was on an old zinc mining site at les avinières, saint-laurent-le-minier, gard, south of france. on the basis of 16s rrna gene sequence similarities, strain chimec512(t) was shown to belong to the genus rhizobium and to be most closely related to rhizobium endophyticum ccge 2052(t) (98.4%), rhizobium tibeticum ccbau ...201525701848
cada of mesorhizobium metallidurans isolated from a zinc-rich mining soil is a p(ib-2)-type atpase involved in cadmium and zinc resistance.mesorhizobium metallidurans stm 2683(t) is a nitrogen-fixing bacterium that nodulates anthyllis vulneraria in mine tailings highly contaminated in zinc, lead and cadmium. to study the mechanisms whereby this bacterium copes with metals, we functionally screened a cosmid genomic library of m. metallidurans for zinc or cadmium tolerance. a cosmid clone harbored a gene encoding p(ib)-type atpase homologous to cada that leads to cadmium and zinc resistance in escherichia coli. the cada protein struc ...201424607711
mesorhizobium delmotii and mesorhizobium prunaredense are two new species containing rhizobial strains within the symbiovar anthyllidis.eight mesorhizobial symbiotic strains isolated from anthyllis vulneraria root-nodules were studied and compared taxonomically with defined mesorhizobium species. all strains presented identical 16s rdna sequences but can be differentiated by multilocus sequence analysis of housekeeping genes (reca, atpd, glnii and dnak). matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analyses separate these strains in two groups and a separate strain. levels of dna-dna relatedness w ...201728238475
examination of secondary metabolites and antioxidant capacity of anthyllis vulneraria, fuchsia sp., galium mollugo and veronica beccabunga.anthyllis vulneraria l., fuchsia sp., galium mollugo l., and veronica beccabunga l. were selected to analyse the phenolic content and the antioxidant activity by ferric ion reducing antioxidant power (frap) and trolox equivalent antioxidant capacity (teac) assays. the highest polyphenol, tannin, and flavonoid contents were measured in fuchsia species (7.40 ± 0.8, 5.62 ± 0.7 and 0.72 ± 0.1 g/100 g dry weight), while the lowest values were detected in anthyllis vulneraria (0.68 ± 0.02, 0.17 ± 0.03 ...201628000504
ancient heavy metal contamination in soils as a driver of tolerant anthyllis vulneraria rhizobial communities.anthyllis vulneraria is a legume associated with nitrogen-fixing rhizobia that together offer an adapted biological material for mine-soil phytostabilization by limiting metal pollution. to find rhizobia associated with anthyllis at a given site, we evaluated the genetic and phenotypic properties of a collection of 137 rhizobia recovered from soils presenting contrasting metal levels. zn-pb mine soils largely contained metal-tolerant rhizobia belonging to mesorhizobium metallidurans or to anothe ...201727793823
hidden founder effects: small-scale spatial genetic structure in recently established populations of the grassland specialist plant anthyllis vulneraria.the long-term establishment success of founder plant populations has been commonly assessed based on the measures of population genetic diversity and among population genetic differentiation, with founder populations expected to carry sufficient genetic diversity when population establishment is the result of many colonists from multiple source populations (the 'migrant pool' colonization model). theory, however, predicts that, after initial colonization, rapid population expansion may result in ...201525892081
prospecting metal-tolerant rhizobia for phytoremediation of mining soils from morocco using anthyllis vulneraria l.the aim of this work was using the legume plant anthyllis vulneraria l. (ecotype metallicolous) as a trap plant, in order to isolate metal-tolerant rhizobial strains from metal-contaminated soils from morocco, with pollution indexes spanning three orders of magnitude. as bioindicator, soil bacterial density was inversely correlated to the pollution index. forty-three bulk soil bacteria and sixty two bacteria from nodules were isolated. the resistance of bacteria from nodules to heavy metals was ...201525315928
the leguminous species anthyllis vulneraria as a zn-hyperaccumulator and eco-zn catalyst resources.anthyllis vulneraria was highlighted here as a zn-hyperaccumulator for the development of a pilot phytoextraction process in the mine site of les avinières in the district of saint-laurent-le-minier. a. vulneraria appeared to hyperaccumulate the highest concentration of zn in shoots with a better metal selectivity relative to cd and pb than the reference zn-hyperaccumulator noccea caerulescens. a bigger biomass production associated to a higher zn concentration conducted a. vulneraria to the hig ...201525253057
new microsatellite markers for anthyllis vulneraria (fabaceae), analyzed with spreadex gel electrophoresis. 201325202507
growth, photosynthetic acclimation and yield quality in legumes under climate change simulations: an updated survey.continued emissions of co2, derived from human activities, increase atmospheric co2 concentration. the co2 rise stimulates plant growth and affects yield quality. effects of elevated co2 on legume quality depend on interactions with n2-fixing bacteria and mycorrhizal fungi. growth at elevated co2 increases photosynthesis under short-term exposures in c3 species. under long-term exposures, however, plants generally acclimate to elevated co2 decreasing their photosynthetic capacity. an updated sur ...201425113447
metal accumulation strategies in plants spontaneously inhabiting zn-pb waste deposits.metal (zn, pb, cd, cu, ni, cr) accumulation in shoots of 38 plant species spontaneously colonizing three zn-pb waste deposits in southern poland was studied in order to find out if the age of the waste (30-130 years) or its type (slag or flotation residues) influence metal content in plants and to identify species potentially suitable for biomonitoring and phytoremediation. the total metal concentrations in the waste upper layers ranged from 7300 to 171,790 mg kg(-1) for zn, from 1390 to 22,265 ...201424793328
elements uptake by metal accumulator species grown on mine tailings amended with three types of biochar.mine tailings are of great concern due to the risk their toxic inorganic elements pose to the environment. the application of biochar as an amendment may be a solution to reduce the risk of pollutant diffusion. the main purpose of the research was to verify the effects of different types of biochar produced from different feedstocks (pruning residues, fir tree pellets and manure pellets) on changing the substrate conditions to promote plant growth for the phytostabilization of mine tailings. the ...201424056450
molecular and phenotypic characterization of strains nodulating anthyllis vulneraria in mine tailings, and proposal of aminobacter anthyllidis sp. nov., the first definition of aminobacter as legume-nodulating bacteria.bacterial strains from zn-pb mine tailings were isolated by trapping with anthyllis vulneraria, a legume-host suitable for mine substratum phytostabilisation. sequence analysis of the 16s rrna gene and three housekeeping genes (atpd, dnak and reca) showed that they were related to those of the genus aminobacter. dna-dna relatedness of representative isolates supported the placement of novel strains in aminobacter as a new species. phenotypic data emphasize their differentiation from the other re ...201222221859
synergistic effects of an extreme weather event and habitat fragmentation on a specialised insect herbivore.habitat fragmentation is considered to be one of the main causes of population decline and species extinction worldwide. furthermore, habitat fragmentation can decrease the ability of populations to resist and to recover from environmental disturbances such as extreme weather events, which are expected to occur at an increasing rate as a result of climate change. in this study, we investigated how calcareous grassland fragmentation affected the impact of the climatically extreme summer of 2003 o ...200919002504
triterpenoid sapogenin from anthyllis vulneraria l.triterpene pentacyclic sapogenin, 3beta,22beta,24-trihydroxy-olean-12-ene, soyasapogenol b, was isolated for the first time from the anthyllis vulneraria l.. identification and structure assignment was performed by 1h and 13c nmr spectroscopy.200111693735
pollination ecology of anthyllis vulneraria subsp. vulgaris (fabaceae): nectar robbers as pollinators.this paper examines the hypothesis that nectar robbing can affect plant reproductive success either positively or negatively. to this end, i investigated various aspects of the pollination ecology of a population of the herb anthyllis vulneraria subsp. vulgaris in northwest spain over 5 yr. by observing floral visitors, i found that the most important pollinator species was the long-tongued bee anthophora acervorum, which accounted for ∼45% of recorded insect visits. however, just over 45% of vi ...200010898775
heat effects on seeds and rhizomes of a selection of boreal forest plants and potential reaction to fire.to analyse the potential reaction to firegenerated heat pulses, seeds of 12 species of plants and rhizomes of three species were exposed to elevated temperatures for 10 min. the tested material split into three groups with respect to heat tolerance: (1) the rhizomes, for which the lethal temperatures were in the range 55-59° c; (2) the seeds of most of the species tested, for which the lethal temperatures were in the range 65-75° c; (3) the seeds of two species of leguminosae and three species o ...199328313665
flavonoid pigments in chalkhill blue (lysandra coridon poda) and other lycaenid butterflies.nine flavonoids, namely, kaempferol, kaempferol 7-rharanoside, kaempferol 3-rhamnoside, kaempferol 3-glucoside, kaempferol 3-glucoside, 7-rhamnoside, quercetin 3-glucoside, quercetin 3,7-diglucoside, isorhamnetin 3-glucoside, and isorhamnetin 3,7-diglucoside, have been identified in the body and wings of the chalkhill blue butterflylysandra coridon poda. flavonoids have also been found in 15 of a further 17 lycaenid species examined. analysis of the two-dimensional paper chromatographic flavonoi ...198724301889
[a new natural quercetin heteroside extracted from anthyllis vulneraria l. (leguminosae)]. 19724631530
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