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hysterothylacium aduncum (rudolphi, 1862) infection in cod from the oslofjord: seasonal occurrence of third- and fourth-stage larvae as well as adult worms.hysterothylacium aduncum infections in cod from the oslofjord were followed for 1 year. the recovered worms were grouped according to developmental stage [third-stage larvae (l3), fourth-stage larvae (l4) and adult worms] and according to location (in the gut or encapsulated on the viscera). the cod were found to be infected with h. aduncum (all stages lumped) throughout the whole year, with the prevalence approaching 100%. the abundance of the parasites (mean number per fish studied), however, ...19938469672
allergenic cross-reactivity between third stage larvae of hysterothylacium aduncum and anisakis simplex. 19989580257
new data on the early development of hysterothylacium aduncum (nematoda, anisakidae).this note reports on incidental observations of the early development of the third-stage larvae of hysterothylacium aduncum from gadid fishes. gravid h. aduncum females were collected from pollachius virens, pollachius pollachius. gadus morhua, and molva molva in norwegian waters. the eggs were incubated at 20 per thousand salinity and 5 c. spontaneous hatching of third-stage larvae was observed 10-25 days after egg deposition. these larvae were long lived and could infect acartia tonsa copepods ...19989645866
characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal dna sequences.larvae of three species of anisakid nematode from fish, anisakis simplex, hysterothylacium aduncum and contracaecum osculatum, were characterised genetically using a molecular approach. the nuclear ribosomal dna region spanning the first internal transcribed spacer, the 5.8s gene and the second internal transcribed spacer was amplified and sequenced. the lengths of the first and second internal transcribed spacer sequences of the three species ranged from 392 to 449 bp and 262 to 347 bp, respect ...19989925272
ascaridoidea: a simple dna assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds.eleven species belonging to superfamily ascaridoidea, which infect marine and freshwater fish, mammals, and fish-eating birds, were analyzed using a pcr-rflp method. the following species were investigated: anisakis pegreffi, a. physeteris, and a. simplex (parasites of fish and mammals), contracaecum osculatum, c. radiatum, and c. rudolphi (parasites of mammals and fish-eating birds), hysterothylacium aduncum (a parasite of fish), porrocaecum angusticolle, p. crassum, p. depressum, and p. ensica ...200212243736
[application of the pcr-rflp technique for the species-specific identification of nematodes involved in human anisakiasis].we intended to develop a molecular test allowing a species-specific identification of the anisakid human parasite independently of its evolutive stage.200415171827
life cycle strategy of hysterothylacium aduncum to become the most abundant anisakid fish nematode in the north sea.the present study demonstrates the influence of physical systems (mixed and stratified waters) on the occurrence and distribution of the anisakid nematode hysterothylacium aduncum in commercially important gadiform fish species from the central and northern north sea. stratified waters are essential in structuring marine food webs and are therefore also important for the transfer of fish parasites to their host species. during two research cruises in 2001, 308 haddocks (melanogrammus aeglefinus) ...200515986247
a recombinant enolase from anisakis simplex is differentially recognized in natural human and mouse experimental infections.a 1,963-bp cdna was isolated from an anisakis simplex cdna library by immunoscreening with a hyperimmune rabbit serum raised against a crude extract of a. simplex l3 larvae. the open reading frame encodes a putative protein of 436 amino acid residues, which exhibits high similarity (70-80%) to enolase molecules from various other organisms, including helminth parasites. after subcloning and expression of the a. simplex cdna in pgex-4t-3, the resulting glutathione s-transferase fusion protein, pu ...200616049725
helminth communities of european eels anguilla anguilla (linnaeus, 1758) from the vistula lagoon and puck bay, poland.within 2001-2002 a total of 621 eel anguilla anguilla (l., 1758) (488 from the vistula lagoon and 133 from the puck bay) were examined. fifteen parasite taxa were recovered: pseudodactylogyrus anguillae (yin et sproston, 1948), brachyphallus crenatus (rudolphi, 1802), deropristis inflata (molin, 1859), diplostomum spp., bothriocephalus claviceps (goeze, 1782), proteocephalus macrocephalus (creplin, 1825), anguillicola crassus (kuwahara, niimi et itagaki, 1974), camallanus lacustris (zoega, 1776) ...200516838624
colonization of the round goby, neogobius melanostomus (gobiidae) by parasites in the new environment of the gulf of gdańsk (southern baltic).the round goby (neogobius melanostomus) is a non-indigenous species in the baltic sea, introduced to its waters (the gulf of gdańsk) from the black, azov, and caspian seas. for this reason, an attempt was made to determine the species' parasitic fauna in its new environment. within 1994-2000, a total of 201 round goby specimens caught in the gulf of gdańsk were examined. the parasites found represented protozoans (trichodina domerguei domerguei), digeneans (diplostomum spp. metacercariae), cesto ...200216888941
metazoa parasites of the invasive round goby apollonia melanostoma (neogobius melanostomus) (pallas) (gobiidae: osteichthyes) in the gulf of gdańsk, baltic sea, poland: a comparison with the black sea.the known metazoa parasite fauna of the invasive round goby apollonia melanostoma (formerly neogobius melanostomus) consists of 12 species. the core of the parasite fauna comprises two species: cryptocotyle concavum and diplostomum spathaceum; secondary species are absent; satellite species include cercariae gen. sp. and ergasilus sieboldi; rare species are acanthocephalus lucii, anguillicola crassus, bothriocephalus sp., dichelyne minutus, hysterothylacium aduncum, pomphorhynchus laevis, piscic ...200717048001
parasite fauna of the eel, anguilla anguilla (linnaeus, 1758), from the polish part of the vistula lagoon.eel is one of the most valuable commercial fish species of the vistula lagoon. for this reason its infection by non-indigenous species of parasites is the subject of the interest of sciencists.200617120993
parasitofauna of the nine-spined stickleback from the gulf of gdańsk and the mouth of dead vistula.four species, one subspecies and one parasite marked to the genus were collected from the nine-spined stickleback pungitius pungitius l. from the gulf of gdańsk and the mouth of dead vistula. nine-spined stickleback was noted as a new host in polish coastal water for five parasites: glugea anomala (microsporidia), diplostomum spathaceum (digenea-metacercariae) and apatemon sp. (digenea-incysted metacercariae), hysterothylacium aduncum (nematoda-third stage larvae) and thersitina gasterostci (cop ...200016886342
anisakid parasites of two forkbeards (phycis blennoides and phycis phycis) from the eastern mediterranean coasts in tunisia.two gadiform species with a successive bathymetric and an ecological and economical importance in the mediterranean fishing industry, phycis blennoides and phycis phycis, were selected for the present study. a total of 592 fresh specimens belonging to the gadiformes genus were obtained from local commercial fisheries. the investigation was centred on anisakid parasites of 272 specimens of the greater forkbeard (p. blennoides) and 320 of the forkbeard (p. phycis) captured off the mediterranean co ...200616847607
genetic variability in hysterothylacium aduncum, a raphidascarid nematode isolated from sprat (sprattus sprattus) of different geographical areas of the northeastern atlantic.species of the genus hysterothylacium are among the most common marine nematode fish parasites in the northern atlantic. due to recent findings of cryptic speciation in other parasitic ascaridoid nematodes, a similar pattern of sibling species was hypothesized also for hysterothylacium aduncum. by investigating a 886- to 890-bp-long genomic dna fragment including its-1, 5.8s rdna and its-2 of 40 specimens of h. aduncum of sprat (sprattus sprattus) of four different biogeographical regions (north ...200717674051
anisakid parasites of two forkbeards (phycis blennoides and phycis phycis) from the mediterranean coasts of andalucía (southern spain).an epidemiological survey of anisakids was carried out on 209 specimens of greater forkbeard (phycis blennoides) and 58 of forkbeard (phycis phycis) captured off the mediterranean coasts of andalucía (southern spain). four species of nematodes were identified: anisakis simplex s.l., anisakis physeteris, hysterothylacium aduncum and hysterothylacium fabri. the total prevalence was 62.06% in the forkbeard and 58.85% in the greater forkbeard. the highest values of prevalence (56.90%), mean intensit ...200616168705
endoparasites of the blue whiting, micromesistius poutassou from north-west spain.the communities of metazoan endoparasites of blue whiting, micromesistius poutassou, in waters of north-west spain were analysed and a geographical comparison made with other localities. four hundred blue whiting collected in july 1999 and september 2000 were examined for parasites, excluding the head and gills. six species were found: anisakis simplex s.l. (l3), a. physeteris (l3), hysterothylacium aduncum (l2 and l3), stephanostomum lophii (metacercaria), s. pristis (adult), and prosorhynchus ...200515831108
hydrolases of hysterothylacium aduncum (nematoda).enzymatic activity is an indicator of an organism's metabolic rate which depends on, i.e., environmental conditions, developmental stage, physiological stage, and sex. the api zym test was applied to compare activities of 19 hydrolases of female and male hysterothylacium aduncum.200717912803
helminths in leuciscus cephalus from the river adda, lombardy, northern italy.chubs (leuciscus cephalus) were caught at various locations along the river adda and from lake of como (lombardy, northern italy) and endoparasitic helminths were studied in order to integrate the knowledge on distribution of helminth fauna of chubs in lombardy and to investigate on the structure of its parasite infracommunities with respect to different habitat typologies. the following species of parasites were isolated: pomphorynchus laevis, allocreadium markewitschi, parasymphylodora markewi ...200315267000
[investigation on the nematode of hysterothylacium aduncum (anisakidae) from bohai sea and yellow sea in china].to investigate hysterothylacium aduncum (anisakidae) infection in marine fishes from bohai sea and yellow sea.200718441985
parasitic helminth fauna of the cutlass fish, trichiurus lepturus l., and the differentiation of four anisakid nematode third-stage larvae by nuclear ribosomal dna sequences.the helminth fauna of the gastrointestinal tract and abdominal cavity of cutlass fish, trichiurus lepturus l., off the taiwanese coast of the north-western pacific was investigated. the following helminths were found: (1) nematodes--anisakis simplex, hysterothylacium aduncum, porrocaecum decipiens, raphidascaris trichiuri; (2) digeneans--adult lecithochirium trichiuri; and (3) cestodes-plerocercoids of proteocephalus spp. the third-stage larvae of these four anisakid nematodes were characterized ...200415133659
in vitro development of the fish parasite hysterothylacium aduncum from the third larval stage recovered from a host to the third larval stage hatched from the egg.anisakids are parasitic nematodes of fish worldwide, producing economic and human health concerns. it is thus important to ascertain their in vitro life cycle in laboratory studies. here we describe the in vitro development of third-stage larvae (l3) of hysterothylacium aduncum isolated from blue whiting micromesistius poutassou, to the hatching of l3 from eggs obtained from h. aduncum worms grown in glit medium (a modified mixture of yaeger's lit [liver infusion tryptose] and grace's media) at ...200415038450
determination of ige in the serum of patients with allergic reactions to four species of fish-parasite anisakids.this study investigates cross-reactions between somatic and metabolic antigens of various anisakids in the serum of patients with allergic processes. twenty patients with allergic reactions after eating fish were studied using the skin-prick test for sensitivity to four species of anisakids. ige was also determined, by blotting, in the serum of these patients when confronted with somatic and metabolic antigens of anisakis simplex s.l. and hysterothylacium aduncum, and somatic antigens of a. phys ...200312968392
metazoan parasites and food composition of juvenile etmopterus spinax (l., 1758) (dalatiidae, squaliformes) from the norwegian deep.a total of 37 juvenile etmopterus spinax from the norwegian deep were examined for stomach contents and metazoan ecto- and endoparasites. these squaliform elasmobranchs were caught by benthopelagic net in may 2001 at a depth of 194-214 m. the euphausiid meganyctiphanes norvegica and the teleost maurolicus muelleri were the principal prey organisms. with increasing total length of e. spinax, the frequency of meganyctiphanes norvegica prey decreased and that of m. muelleri increased. seven differe ...200312632160
in vitro cultivation of hysterothylacium aduncum (nematoda: anisakidae) from 3rd-stage larvae to egg-laying adults.this is the first demonstration of the in vitro development of the 3rd-stage larvae (l3) of hysterothylacium aduncum to the adult. this was achieved in a semi-defined medium that is easy to prepare and to reproduce. the l3, collected from the peritoneal cavity of horse mackerel (trachurus trachurus), were individually inoculated into rpmi-1640 medium +20% heat-inactivated fetal bovine serum (ifbs). it has been demonstrated that the optimum temperature for development is around 13 degrees c and i ...200212458831
growth and emigration of third-stage larvae of hysterothylacium aduncum (nematoda: anisakidae) in larval herring clupea harengus.the growth and emigration of hystherothylacium aduncum in laboratory-reared herring larvae clupea harengus was studied. experimental infections of 36-day-old herring larvae resulted in 126 hosts infected with 306 h. aduncum larvae. regression analyses showed a significant worm emigration from the rectum to the head of the fish, accompanied by an increase in worm body length. the emigration was independent of worm intensity, which suggests an ontogenetic process. some worms departed from this pat ...200011191903
larval anisakids parasitizing the blue whiting, micromesistius poutassou, from motril bay in the mediterranean region of southern spain.a total of 301 blue whiting, micromesistius poutassou risso, 1826, ranging in length from 17 to 28 cm, from motril bay (mediterranean coast, south spain) were examined for anisakid nematodes, as these fish are common items in the spanish mediterranean diet. three anisakid species were morphologically identified with a total prevalence of 10.63%. anisakis simplex s.l. rudolphi, 1809 had a prevalence value of 6.65%, compared with 2.66% for a. physeteris baylis, 1923 and 2.33% for hysterothylacium ...200011138027
larvae and adults of hysterothylacium aduncum (rudolphi, 1802) (nematoda: anisakidae) in fishes and crustaceans in the south west atlantic.hysterothylacium aduncum (rudolphi, 1802) is reported from five fishes and one invertebrate species. third-stage larvae were found in the crustacean themisto gaudichaudii and in mesenteries of the fishes engraulis anchoita and merluccius hubbsi; fourth-stage larvae were recovered from the digestive tract of m. hubbsi and scomber japonicus and adult specimens were obtained from the stomach and intestine of m. hubbsi, s. japonicus, genypterus blacodes and genypterus brasiliensis. nematodes are des ...19989754308
endohelminth parasites from salmonids in intensive culture from southern chile.a total of 228 salmonids (90 oncorhynchus mykiss, 48 oncorhynchus kisutch, and 90 salmo salar) from 8 intensive aquaculture centers in the south of chile were examined for endohelminths parasites between december 2008 and may 2009. the body cavities of 2 o. mykiss were infected by diphyllobothrium sp. plerocercoids (prevalence: 6.7%, mean intensity: 1.0, mean abundance: 0.07) from the lake tarahuin hatchery on the south of chiloé island. also, tetraphyllidean plerocercoids (prevalence: 3.3%, mea ...201020557217
contribution to the knowledge of hysterothylacium aduncum through electrophoresis of the enzymes glucose phosphate isomerase and phosphoglucomutase.a total of 163 hysterothylacium aduncum specimens, obtained from two gadoids and one percid, were studied by electrophoresis of the enzymes glucose phosphate isomerase and phosphoglucomutase. the two loci deviated significantly from the hardy-weinberg equilibrium, both when considering all specimens and when distinguishing the hosts. this could suggest that there is no single species in either case.19989493219
free and bound biotin molecules in helminths: a source of artifacts for avidin biotin-based immunoassays.the avidin-biotin molecular recognition system is widely used in parasite immunology. however, the presence of biotin and/or biotin-containing molecules (bcms) in samples may lead to erroneous results. in the work reported herein we investigated the extent to which biotin and bcms present in helminth extracts may interfere in avidin/biotin-based immunoassays and developed an enzyme-linked immunosorbent assay (elisa) for quantification of these components. in avidin-based elisa using antinematode ...19968875569
larval anisakids (nematoda: ascaridoidea) in horse mackerel (trachurus trachurus) from the fish market in granada (spain).horse mackerel (trachurus trachurus) from the fish market in granada, south spain, were surveyed for anisakid nematodes. the fish came from fishing ports all over the country. larval anisakids were found in 39.4% of the fish examined. in all, 26.1% were infected with third-stage larvae (l3s) of anisakis simplex; 0.3%, with a. physeteris l3s; 31.1%, with hysterothylacium aduncum l3s; and 1.7%, with fourth-stage larvae (l4s) of hysterothylacium sp. horse mackerel from mediterranean sea coast ports ...19968801559
antigenic cross-reactivity in mice between third-stage larvae of anisakis simplex and other nematodes.we used elisa and immunoblotting to investigate antigenic cross-reactivity in mice between third-stage larvae of anisakis simplex and five other nematodes: the ascaridoids ascaris suum, toxocara canis and hysterothylacium aduncum, and the nonascaridoids trichinella spiralis and trichuris muris. two sera were raised against each species (including a. simplex, but excluding a. suum), by infection or by immunization with somatic antigens. serum against a. suum was raised by immunization only. the r ...19968740558
larval anisakids (nematoda:ascaridoidea) in horse mackerel (trachurus trachurus) from the fish market in granada, spain.horse mackerel (trachurus trachurus) from the fish market in granada, southern spain, were surveyed for anisakid nematodes. the fish came from fishing ports all over the country. larval anisakids were found in 39.4% of the fish examined; 26.1% were infected with third-stage larvae (l3s) of anisakis simplex; 0.3%, with a. physeteris l3s; 31.1%, with hysterothylacium aduncum l3s; and 1.7%, with fourth-stage larvae (l4s) of hysterothylacium sp. horse mackerel from mediterranean sea coastal ports (s ...19968740547
[the possibility of completing the life cycle of hysterothylacium aduncum (rudolphi, 1802) and contracaecum rudolphii (hartwich, 1964) (nematoda) at the waters of vistula lagoon].the probabilities of completing the life cycle of hysterothylacium aduncum and contracaecum rudolphii at the water of vistula lagoon was considered. hysterothylacium aduncum is a parasite of some fish species from vistula lagoon and contracaecum rudolphii is common parasite of cormorant phalacrocorax carbo from colony of katy rybackie.200516913529
a comparative study on the parasite fauna of perch, perca fluviatilis l., collected from a freshwater coastal lake, brackish-water baltic sea, and the interconnecting canal.parasitological surveys of freshwater fishes rarely include comparisons between two ecologically different bodies of water. such studies might help to understand processes of establishment of parasite faunas in estuary areas. the results obtained could also provide useful tools for discriminating various fish populations based on the composition of their parasite faunas. the present authors attempted to study such data from resko lake-a freshwater coastal lagoon (6 km2 surface area), and the adj ...200516913502
cross-reactivity between antigens of anisakis simplex s.l. and other ascarid nematodes.a study of the cross-reactivity among somatic and excretory-secretory antigens of the third stage larvae of anisakis simplex s.l. and somatic antigens of other ascarid nematodes (ascaris lumbricoides, a. suum, toxocara canis, anisakis physeteris, hysterothylacium aduncum and h. fabri) was carried out by immunoblotting. it was revealed a high degree of cross-reactivity among ascarids in the 30 and > 212 kda range by using sera against somatic and excretory-secretory antigens of a. simplex s.l. it ...200415224584
the enzymes of glycogen and trehalose catabolism from hysterothylacium aduncum (nematoda: anisakidae).the activity of alpha-amylase, glucoamylase, maltase, trehalase, glycogen phosphorylase and trehalose phosphorylase was measured in extracts from larval and adult hysterothylacium aduncum (rudolphi, 1802), parasitic nematode of marine fish. the content of glycogen and trehalose in the worm's body was also determined. both the hydrolytic and phosphorolytic paths of sugar decomposition are present in h. aduncum. in the larvae glycogen was utilised mainly via the hydrolytic path. in the adults the ...200212418818
parasites of fingerling herring clupea harengus l.: ecology and fine morphology.the parasite fauna of young-of-the-year herring clupea harengus l., off gullmarsfjord and brofjorden, west coast of sweden, was studied between may and october for 4 years, from 1994 to 1997. fifteen species of parasites were found: two protozoa - trichodina sp. and ceratomyxa auerbachi; one species of uncertain affinity - ichthyophonus hoferi; two monogenea - gyrodactylus harengi and pseudanthocotyloides heterocotyle; five digenea - cryptocotyle lingua metacercariae, cercaria pythionike metacer ...200717578600
identification of anisakid nematodes with zoonotic potential from europe and china by single-strand conformation polymorphism analysis of nuclear ribosomal dna.using genetic markers defined previously in the second internal transcribed spacer (its-2) of nuclear ribosomal dna (rdna), isotopic, and non-isotopic polymerase-chain-reaction-coupled single-strand conformation polymorphism (sscp) were utilized to identify each of three anisakid species [anisakis simplex (s.l.), contracaecum osculatum (s.l.), and hysterothylacium aduncum] from different host species and geographical locations in poland and sweden. while subtle microheterogeneity was observed wi ...200717694403
parasite communities of eels anguilla anguilla in freshwater and marine habitats in iceland in comparison with other parasite communities of eels in europe.ninety-five eels from one marine and three freshwater localities in iceland were examined for parasites. twenty species were found, 12 from marine habitat, 12 from freshwater and 4 species were found in both habitats. these are: eimeria anguillae, chilodonella hexasticha, trichodina fultoni, t. jadranica, myxidium giardi, myxobolus kotlani, two zschokkella spp., derogenes varicus, deropristis inflata, diplostonmum sp., plagioporus angulatus, podocotyle atomon, anisakis simplex (larva), eustrongy ...200717886743
parasitation of sea trout (salmo trutta trutta l.) from the spawning ground and german coastal waters off mecklenburg-western pomerania, baltic sea.a total of 52 sea trouts, salmo trutta trutta, were studied for parasites, originating from german freshwater streams and coastal waters of the baltic sea. while 35 specimens were caught mainly close to the shoreline in the baltic sea, 17 were sampled during their spawning migration in warnow river and other neighboring rivers in mecklenburg-western pomerania. a total of 12 different metazoan parasite species were found in sea trout originating from the baltic sea, including five digeneans, two ...201626374539
nematodes parasitizing trachurus trachurus (l.) and boops boops (l.) from algeria.a total of 455 boops boops (linnaeus, 1758) and 953 trachurus trachurus linnaeus, 1758 from the east coast of algeria were examined for their parasitic nematoda. two hundred ninety-five specimens of larval stages l3 and l4 were collected from the peritoneal cavity of these two examined fishes. photonic and scanning electronic microscopy (sem) studies were performed on these larvae specimens in order to characterize their morphology. two different species of nematoda (anisikidae) were identified: ...201526220559
the fishing area as a possible indicator of the infection by anisakids in anchovies (engraulis encrasicolus) from southwestern europe.a study was conducted on the parasitization by anisakids of european anchovies (engraulis encrasicolus) from the eastern atlantic (gulf of cádiz and strait of gibraltar) and western mediterranean (ligurian sea, gulf of lion, catalonia coast and nw alborán sea) throughout 1998 and 1999. the anisakids detected were identified as third larval stages of anisakis larva type i and hysterothylacium aduncum. global prevalence was 9.4% for anisakis and 24.5% for h. aduncum. analysis of the origin of the ...200919162359
species composition and infection dynamics of ascaridoid nematodes in barents sea capelin (mallotus villosus) reflecting trophic position of fish host.capelin (mallotus villosus) is among the most abundant fish species in the barents sea, and represents a critical food source for many predators in the area including atlantic cod and harp seal. in norway, the fish is of economic importance since whole capelin and roe are valuable export products. despite its economic and ecological importance, the parasites of barents sea capelin are poorly known. however, the presence of parasites in the edible parts may adversely affect product quality and co ...201627473836
survey of anisakis sp. and hysterothylacium sp. in sardines and anchovies from the north adriatic sea.the occurrence of larval anisakidae and raphidascarididae in anchovies and sardines from the north adriatic sea has been estimated. anisakis pegreffii and hysterothylacium aduncum were reported, with low prevalence values. in brief, a total amount of 7650 fish specimens collected between september 2011 and 2012 were analysed using three different inspection analyses: a visual inspection of the coelomic cavity, an examination of the viscera exploiting the positive hydro-tropism of the larvae (mod ...201525662709
molecular identification of anisakis and hysterothylacium larvae in marine fishes from the east china sea and the pacific coast of central japan.anisakiasis is a human disease caused by the accidental ingestion of larvae belonging to the family anisakidae. three fish species, the small yellow croaker pseudosciaena polyactis, the mackerel pneumatophorus japonicus and the hairtail trichiurus haumela are important source for food products in the east china sea. the prevalence and the identification of anisakidae larvae in these fishes will benefit the prevention and control of anisakiasis. in this study, fish samples were obtained from fish ...201525617845
the specific identification of anisakid larvae from fishes from the yellow sea, china, using mutation scanning-coupled sequence analysis of nuclear ribosomal dna.nematodes of the family anisakidae parasitize fish, mammals, birds and reptiles, with the larval stages of some species causing severe clinical disease in humans. therefore, the accurate identification of anisakid nematodes is a key component of disease surveillance and control. an epidemiological survey of 123 fishes comprising eight different species from the yellow sea in china revealed that more than 25% of fish were infected with the larvae of anisakids, 200 third-stage larvae (l3s) were co ...200717604951
parasites as biological tags for stock discrimination of beaked redfish (sebastes mentella): parasite infra-communities vs. limited resolution of cytochrome markers.the use of parasites as biological tags for discrimination of fish stocks has become a commonly used approach in fisheries management. metazoan parasite community analysis and anisakid nematode population genetics based on a mitochondrial cytochrome marker were applied in order to assess the usefulness of the two parasitological methods for stock discrimination of beaked redfish sebastes mentella of three fishing grounds in the north east atlantic. multivariate, model-based approaches demonstrat ...201627104735
co(2)-fixing enzymes during moulting from third larval to fourth larval stage of anisakis simplex and hysterothylacium aduncum (nematoda: anisakidae).the fixing of co(2) is an important metabolic process for many organisms. in the anisakid nematodes, co(2) has been shown to be necessary for their development, at least in vitro. the presence of co(2) stimulates the moulting (m3) of the larvae from the third (l3) to the fourth (l4) stage and prolongs the survival, at least, in vitro. we determined the activity of co(2)-fixing enzymes, common to many organisms, in two anisakids: anisakis simplex, a parasite of cetaceans, and hysterothylacium adu ...200515864647
multiplex pcr for the identification of anisakis simplex sensu stricto, anisakis pegreffii and the other anisakid nematodes.a multiplex pcr method was established for the rapid identification of anisakis simplex sensu stricto, a. pegreffii, a. physeteris, pseudoterranova decipiens, contracaecum osculatum and hysterothylacium aduncum. the sequence alignment of the internal transcribed spacer 1 region (its-1) between a. simplex s. str. and a. pegreffii showed a high degree of similarity, but only two c-t transitions were observed. to differentiate a. simplex s. str. from a. pegreffii, an intentional mismatch primer wit ...200817962067
anisakid parasites of the pouting (trisopterus luscus) from the cantabrian sea coast, bay of biscay, spain.an epidemiological survey was undertaken of anisakids in 139 specimens (length: 13.2-24.5 cm) of pouting or bib (trisopterus luscus) captured off the coast of northern spain in the cantabrian sea. third-stage larvae of two species of nematodes, anisakis larvae type i and hysterothylacium aduncum, were isolated. one adult female h. aduncum was also detected in the intestine of one pouting. total prevalence of anisakids was 88.5%. hysterothylacium aduncum and anisakis showed, respectively, prevale ...200818593503
presence of anisakid larvae in the european anchovy, engraulis encrasicolus, fished off the tyrrhenian coast of central italy.a survey was carried out to determine the prevalence of anisakid nematode larvae in european anchovy (engraulis encrasicolus) fished off the tyrrhenian coast of central italy. from february through july 2012, 1,490 specimens of e. encrasicolus caught in three different fishing areas (off civitavecchia, anzio, and gaeta in the northern, central, and southern lazio region of italy, respectively) were tested for the presence of anisakid larvae, both by visual microscopic inspection and enzymatic di ...201323992513
[helminths of antarctic fishes].antarctic fishes are represented by sharks, skates (chondrichthyes) and bony fishes (teleostei). teleosts play an important role in the completion of life cycles of many helminth species. they serve as either definitive or intermediate and paratenic hosts. chondrichthyes are definitive hosts only. seventy three helminth species occur as the adult stage in fishes: digenea (45), cestoda (14), nematoda (6), acanthocephala (8), also, 11 larval stages of cestoda (7) and nematoda (4) are known, togeth ...200818664106
specific pcr assays for the identification of common anisakid nematodes with zoonotic potential.based on the sequences of the internal transcribed spacers (its-1 and its-2) of nuclear ribosomal dna (rdna) for six taxa of anisakids, namely, anisakis simplex (s.s.), anisakis typica, anisakis pegreffii, hysterothylacium aduncum, hysterothylacium sp, and contracaccum osculatum c, specific primers were designed in the its-1 and/or its-2 for each of the six anisakid taxa. these specific primers were used to develop polymerase chain reaction (pcr) tools for the identification of these anisakid ta ...200818758823
hysterothylacium aduncum, the only anisakid parasite of sardines (sardina pilchardus) from the southern and eastern coasts of spain.an epidemiological study was carried out on the anisakids in sardines (sardina pilchardus) from the southern (atlantic and mediterranean) and eastern coasts of spain. length of fish was from 12.2-21.0 cm. the anisakids found were identified as the third larval stage (l3) of hysterothylacium aduncum, with a total prevalence of 11.85%. prevalence within the host was 9.64% in viscera and 4.69 % in muscle. the highest infection parameters were found in fish from the east coast (western balearic sea) ...200818784942
comparative infectivity of three larval nematode species in three different salmonids.host specificity of parasites may depend both on ecological and physiological factors. basic descriptions of the susceptibility/resistance of fish to specific nematodes are needed in order to reveal mechanisms in the host-parasite relation. rainbow trout (oncorhynchus mykiss), brown trout (salmo trutta), and atlantic salmon (salmo salar) were orally infected with larval stages of three different anisakid nematodes-hysterothylacium aduncum, contracaecum osculatum, and anisakis simplex-and parasit ...201323733234
co2-fixing enzymes and phosphoenolpyruvate metabolism in the fish parasite hysterothylacium aduncum (ascaridoidea, anisakidae).co2 stimulates the development of many of the intestinal helminths that are able to fix co2 by means of phosphoenolpyruvate carboxykinase (pepck), such as hysterothylacium aduncum. we determined the activity of co2-fixing enzymes such as pepck and phosphoenolpyruvate carboxylase (pepc), although no significant activity was detected for pyruvate carboxylase or carboxylating-malic enzyme. the former act on phosphoenolpyruvate (pep) to yield oxalacetate. in the helminths studied, pep has a vital ro ...200919750810
molecular characterisation of anisakidae larvae from fish in adriatic sea.in the present study, anisakids from: tuna (thunnus thynnus) fattened in the croatian farm in middle adriatic sea, three different feral fish species caught near tuna farm (trachurus trachurus, scomber japonicus and oblada melanura) and fish marketed in croatia (t. trachurus) were analysed by morphology and molecular methods. larvae were identified to the species level by pcr restriction fragment length polymorphism and characterised by sequencing of nuclear (internal transcribed spacer) and mit ...201222983167
characterization of two monoclonal antibodies raised in btk(xid) mice that recognize phosphorylcholine-bearing antigens from trichinella and other helminths.this study investigated the binding properties of two monoclonal antibodies (mabs us1 and us2) raised in (cba/n x balb/c)f1 (nbf1) btk(xid) male mice. both mabs show unusual specificity for phosphorylcholine (pc)-containing tsl4 antigens of trichinella. specifically, and in contrast to mabs raised in normal mice, us1 and us2 mabs do not bind to artificial pc-protein conjugates and are not inhibited by either free pc or nppc, although us2 was partially inhibited by nppc at high concentration (10( ...200111412384
cathepsin b- and l-like cysteine protease activities during the in vitro development of hysterothylacium aduncum (nematoda: anisakidae), a worldwide fish parasite.proteinases play an important role as virulence factors both in the life-cycle of parasites and in the pathogen-host relationship. hysterothylacium aduncum is a worldwide fish parasite nematode which has been associated with non-invasive anisakidosis and allergic responses to fish consumption in humans. cysteine proteinases have been associated with allergy to plant pollens, detergents and dust mites. in this study the presence of two types of cysteine proteinases (cathepsin b and cathepsin l) d ...201019932193
accumulation of some heavy metals in hysterothylacium aduncum (nematoda) and its host sea bream, sparus aurata (sparidae) from north-eastern mediterranean sea (iskenderun bay).the sea bream's nematode and sparus aurata, sampled from the iskenderun bay, mediterranean, in march 2008 were analyzed by inductively coupled plasma-atomic emission spectrometry for their some heavy metal (cd, cr, cu, fe, hg, mn, mg, pb, and zn) levels. the metal concentrations of the parasites were compared to different organs (liver, muscle, gill, intestine, and skin) of the fish hosts. there were significant differences in cd, cr, cu, fe, mn, zn, hg, mg, and pb concentrations in tissues of f ...201120422284
parasitism between anisakis simplex (nematoda: anisakidae) third-stage larvae and the spotted mackerel scomber australasicus with regard to the application of stock identification.the nematode fauna of 369 spotted mackerel of the species scomber australasicus, collected off the northeastern taiwanese coast of the northwestern pacific, was investigated monthly from april 2004 to march 2005. the following nematode species were recorded: anisakis simplex complex, hysterothylacium aduncum, porrocaecum decipiens and raphidascaris trichiuri. the seasonal variation in the infection with a. simplex third stage larva (l3) was studied throughout the 12 months. the prevalence of a. ...201021211909
molecular characterization of hysterothylacium aduncum (nematoda: raphidascaridae) from different fish caught off the tunisian coast based on nuclear ribosomal dna sequences.larval forms of the genus hysterothylacium have been previously reported in teleost fish from the north african coasts of central mediterranean sea by morphological analysis. in the present study, samples identified morphologically as hysterothylacium aduncum (n = 62), from merluccius merluccius, trachurus mediterraneus and pagellus erythrinus from different geographical locations of the tunisian coasts, were genetically characterised by sequences of the first (its-1), the 5.8s and second (its-2 ...201121519865
proteolytic activity in hysterothylacium aduncum (nematoda: anisakidae), a fish gastrointestinal parasite of worldwide distribution.proteases have a significant role in the life cycle of parasites and the pathogen-host relationship, being regarded as important virulence factors. in the parasitic nematode hysterothylacium aduncum proteolytic activity was measured during in vitro development from third larval stage (l3) to mature adult, using dq red casein as a fluorogenic substrate. proteolytic activity was detected in all the developmental stages studied and at all ph values within the range employed (2.0-7.5). the assay wit ...201121802207
survey for the presence of ascaridoid larvae in the cinnamon flounder pseudorhombus cinnamoneus (temminck & schlegel) (pleuronectiformes: paralichthyidae).the cinnamon flounder pseudorhombus cinnamoneus is a frequently consumed marine fish in china. however, the occurrence of ascaridoid larvae in p. cinnamoneus remains unclear. in the present study, a total of 85 p. cinnamoneus caught from the yellow sea (off shidao, 36°52'57″n, 122°26'42″e) in 2011, which is located between mainland china and the korean peninsula, was investigated for ascaridoid larval infection. four ascaridoid larval types, including anisakis type i of berland (1961), hysteroth ...201727768930
macroparasites of allis shad (alosa alosa) and twaite shad (alosa fallax) of the western iberian peninsula rivers: ecological, phylogenetic and zoonotic insights.samples of anadromous alosa alosa (clupeidae) (n = 163) and alosa fallax (clupeidae) (n = 223), caught in western iberian peninsula rivers from 2008 to 2013, were examined for buccal, branchial and internal macroparasites, which were identified using morphological and molecular methods. alosa alosa were infected with anisakis simplex s.s., anisakis pegreffii, hysterothylacium aduncum, rhadinorhynchus pristis, mazocraes alosae, hemiurus appendiculatus, ceratothoa italica and an unidentified ergas ...201526141433
anisakid nematodes of greenland halibut reinhardtius hippoglossoides from the barents sea.parasitic nematodes from the body cavity of 94 greenland halibut (reinhardtius hippoglossoides) caught in the barents sea were examined. nematode species were identified using both morphological and molecular methods. four morphospecies were found: anisakis simplex s.l., contracaecum osculatum s.l., pseudoterranova decipiens s.l., and hysterothylacium aduncum. the most prevalent were c. osculatum s.l. (89.4%) and a. simplex s.l. (86.2%). mean intensities for these 2 species were 7.8 and 45.2, re ...201323536987
presence of anisakis simplex (rudolphi, 1809 det. krabbe, 1878) and hysterothylacium aduncum (rudolphi, 1802) (nematoda; anisakidae) in runts of farmed atlantic salmon, salmo salar l.one hundred farmed atlantic salmon, salmo salar l., were examined for the presence of nematodes by digestion of tissue in hcl-pepsin solution. all fish were sampled from one cage in a fish farm on the norwegian south-west coast. fifty harvest quality salmon, that is, salmon for human consumption (mean 5.4 kg, variation 3.0-7.6 kg), were sampled at the processing line while 50 salmon runts (mean 1.1 kg, variation 0.4-1.8 kg), discarded due to poor performance, were sampled from the discard bin af ...201423488892
host size-dependent anisakid infection in baltic cod gadus morhua associated with differential food preferences.a significant increase in the infection level of baltic cod gadus morhua with the anisakid nematode larvae contracaecum osculatum and pseudoterranova decipiens has been recorded during recent years due to the expanding local population of grey seals halichoerus grypus, which act as final hosts for these parasites. here, we report from an investigation of 368 cod (total length [tl] 6-49 cm; caught in ices subdivision 25) that the infection level of juvenile cod (tl 6-30 cm) with larvae of c. oscu ...201627304871
anisakid nematodes in beaked redfish (sebastes mentella) from three fishing grounds in the north atlantic, with special notes on distribution in the fish musculature.parasitic anisakid nematodes commonly occur in the musculature and visceral organs of many fish species from the north atlantic. in this respect, the presence of the third stage larvae of anisakis spp. in the fish musculature may pose a potential consumer hazard due to the parasite's ability to cause anisakidosis. thus, knowledge on the occurrence and distribution of these potentially zoonotic parasites in the commercially important north atlantic fish species is crucial in order to evaluate and ...201525498327
annual and spatial variability in endo- and ectoparasite infections of north sea cod (gadus morhua linnaeus, 1758) larvae, post-larvae and juveniles.a parasitological investigation was performed on a total of 5380 atlantic cod larvae, post-larvae and small juveniles sampled from the north sea during a period of five years. the copepod caligus elongatus (von nordmann, 1832) and the nematode hysterothylacium aduncum (rudolphi, 1802) were found at a relatively high prevalence of infection (4.6% and 5.2%, respectively). the infection by both parasites showed annual and spatial variability. c. elongatus showed a higher prevalence in 1992 compared ...201424827100
direct parasitologial diagnosis of infection with hysterothylacium aduncum in a patient with epigastralgia.anisakis parasitization has been on the rise because some factors, like increased interest in dishes consisting of raw fish. we report a case of epigastralgia with direct diagnosis by endoscopy, which futher study pointed out h. aduncum as causal agent, a anisakis which is rarely involved in human anisakiasis.201526561913
molecular identification of hysterothylacium aduncum specimens isolated from commercially important fish species of eastern mediterranean sea using mtdna cox1 and its rdna gene sequences.the presence of a raphidascarid parasitic nematode hysterothylacium aduncum (rudolphi, 1802) in two sparid fish (sparus aurata and diplodus vulgaris) and one soleid fish (solea solea) was investigated in this study. a total of 868 individuals; 385 s. aurata, 437 d. vulgaris and 46 s. solea were collected from the mersin bay between february 2013 and january 2014 and examined. variations in the prevalence, mean intensity, and mean abundance of the parasite were 14.55%, 2.05, and 0.30 for s. aurat ...201525543079
hysterothylacium aduncum (nematoda, anisakidae) with a new host record from the common sole solea solea (soleidae) and its role as a biological indicator of pollution.hysterothylacium aduncum (nematoda, anisakidae) was isolated from the intestine of the common sole solea solea (family, soleidae) collected from coasts along alexandria city at the mediterranean sea in egypt, during the period from may to september 2013. light and scanning electron microscopy revealed that this nematode parasite belongs to the family anisakidae in the genus hysterothylacium. the type species is named h. aduncum, based on the presence of three interlocked lips with the interlabiu ...201525468378
new host and locality records of two nematode parasites dujardinnascaris mujibii (heterocheilidae) and hysterothylacium aduncum (anisakidae) from the common seabream pagrus pagrus: a light and scanning electron microscopic study.in the present study, the morphology and morphometric characterization of dujardinnascaris mujibii (heterocheilidae) and hysterothylacium aduncum (anisakidae), new nematode parasites infecting the sea bream pagrus pagrus (osteichthyes, sparidae), were described for the first time from the gulf of suez and hurghada city of the red sea, egypt. ninety-eight (70 %) and 62 (44.2 %) out of 140 of the examined fish were naturally infected with these nematodes, respectively. the infection was investigat ...201323315190
occurrence and molecular characterization of hysterothylacium aduncum (nematoda: anisakidae) from merlangius merlangus euxinus and trachurus trachurus off the turkish coast of black sea.a total of 286 larval forms of hysterothylacium aduncum were collected from merlangius merlangus euxinus and trachurus trachurus captured at different sites of the black sea coast of turkey. prevalence of h. aduncum in m. merlangus euxinus and t. trachurus was 37.4 and 29.3 %, respectively. the fourth-stage larvae from m. merlangus euxinus and t. trachurus of h. aduncum were characterized genetically using a molecular approach. the ribosomal dna (rdna) internal transcribed spacer (its) region (i ...201323239095
metazoan parasite fauna of migrating common garfish, belone belone (l.), in the baltic sea.a total of 35 common garfish, belone belone (linnaeus, 1761), were studied for metazoan parasites on their spawning grounds in the western baltic sea. nine parasite species were found, and six new locality records could be established for german coastal waters (axine belones, monogenea; proteocephalus sp., cestoda; anisakis simplex (s.s.), contracaecum rudolphii a and hysterothylacium aduncum, nematoda; echinorhynchus gadi, acanthocephala). for the first time, the marine ectoparasite a. belones ...201829351069
saduria entomon infected with hysterothylacium aduncum found in situ in the stomach of cod (gadus morhua) from the baltic sea.the parasite fauna of cod (gadus morhus) is well described, but the life cycles of baltic cod parasites are known only in general terms. invertebrates commonly found in the stomach of cod are recognized as intermediate hosts in the life cycles of nematodes or acanthocephalans. the aim of this study was to determine the source of infection of baltic cod with parasites found in situ in invertebrates present in the cod stomach. our results indicate that saduria entomon is both a source of infection ...201729021004
collagenolytic activity related to metalloproteases (and serine proteases) in the fish parasite hysterothylacium aduncum (nematoda: anisakidae).proteases play a vital role in both the life cycle of parasites and the parasite-host relationship and are considered important virulence factors. in the present study, the presence of proteases with collagenolytic activity was investigated in the fish nematode hysterothylacium aduncum during in vitro development. collagenolytic activity was found in all studied developmental stages of the nematode (third [l3] and fourth [l4] larval stages and adults). in l3, the activity was maximum at ph 6.5 a ...201020662369
parasite communities of european perch, perca fluviatilis l. (actinopterygii: perciformes: percidae) from lake łebsko (central coast, poland).communities of parasites of european perch from lake łebsko were studied and compared with similar communities from the polish coastal zone. parasites comprised 18 autogenic and 5 allogenic species. most individual parasites belonged to allogenic species and were in larval stages. the majority of specimens were eye parasites with tylodelphys clavata as the eudominant species. the dominant species, acanthocephalus lucii, belongs to the intestine parasite community. three marine species were found ...201324171303
species richness and diversity of the parasites of two predatory fish species - perch (perca fluviatilis linnaeus, 1758) and zander (sander lucioperca linnaeus, 1758) from the pomeranian bay.pomeranian bay as an ecotone is a transition zone between two different biocenoses, which is characterized by an increase in biodiversity and species density. therefore, pomeranian bay is a destination of finding and reproductive migrations of fish from the rivers entered the area. the aim of the study was to compare parasitic fauna of two predatory fish species from the pomeranian bay, collected from the same fishing grounds at the same period. a total of 126 fish studied (53 perches and 73 zan ...201526342503
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