| [effect of tremulacin on actions of srs-a and histamine]. | the effect of tremulacin (trc) extracted from mao bai yang (folia populus tomentosa carr) on actions of srs-a and histamine were investigated by using isolated guinea pig ileum and spectrofluorometric assay. trc was found to inhibit the contraction of isolated guinea pig ileum induced by histamine and srs-a, in a dose-dependent manner with ic50 of 1.78 x 10(-4) mol.l-1 and 2.51 x 10(-4) mol.l-1, respectively. trc at the dose of 10(-4) mol.l-1 inhibited srs-a release from sensitized isolated guin ... | 1995 | 7544944 |
| stable and specific expression of 4-coumarate:coenzyme a ligase gene (4cl1) driven by the xylem-specific pto4cl1 promoter in the transgenic tobacco. | the ability of 4-coumarate:coenzyme a ligase promoter from populus tomentosa (pto4cl1p) to drive expression of the gus reporter gene and 4-coumarate:coenzyme a ligase gene in tobacco has been studied using transgenic plants produced by agrobacterium-mediated transformation. intense gus histochemical staining was detected in the xylem of stem in transgenic tobacco plants carrying the 1140 bp pto4cl1p promoter. to further investigate the regulation function of the tissue-specific expression promot ... | 2004 | 15266121 |
| overexpression of mtld gene in transgenic populus tomentosa improves salt tolerance through accumulation of mannitol. | the mtld gene encoding mannitol-1-phosphate dehydrogenase, which catalyzes the biosynthesis of mannitol from fructose, was cloned from escherichia coli and transferred to poplar (populus tomentosa carr.) through agrobacterium-mediated transformation. the transgenic plants were screened and selected on murashige and skoog (ms) medium containing 30-50 mg l(-1) kanamycin and verified by polymerase chain reaction (pcr) and southern blotting. expression of the gene led to synthesis and accumulation o ... | 2005 | 16076776 |
| lead and cadmium in leaves of deciduous trees in beijing, china: development of a metal accumulation index (mai). | lead and cadmium uptake was investigated for common deciduous street trees in beijing in this study. species having cd accumulation included populus tomentosa, sophora japonica and catalpa speciosa. p. tomentosa had the highest ratios between leaf and soil cd (0.848), followed by s. japonica (0.536), c. speciosa (0.493), paulownia tomentosa (0.453) and juglans regia (0.415). pb levels were high in leaves of c. speciosa, j. regia and pa. tomentosa. s. japonica had the highest ratio between leaf p ... | 2007 | 16797111 |
| heterologous expression and characterization of a proxidomal ascorbate peroxidase from populus tomentosa. | the present study reported for the first time, cloning, expression and characteristics of a proxidomal apx gene (ppapx) from populus tomentosa. the ppapx gene encodes a protein of 287 amino acid residues with a calculated molecular mass of 31.58 kda. the over-expressed recombinant ppapx protein showed high activity towards the substrates ascorbate aicd (asa) and h(2)o(2). at fixed asa concentrations, the k (m) and v (max) values were 0.12 +/- 0.01 mm and 23.4 +/- 4.2 mmol/min mg for h(2)o(2). an ... | 2009 | 17899442 |
| functional analysis of 5' untranslated region of a tir-nbs-encoding gene from triploid white poplar. | genome-wide analyses have identified a set of tir-nbs-encoding genes in plants. however, the molecular mechanism underlying the expression of these genes is still unknown. in this study, we presented a tir-nbs-encoding gene, ptdrl02, that displayed a low level of tissue-specific expression in a triploid white poplar [(populus tomentosa x p. bolleana) x p. tomentosa], and analyzed the effects of the 5' untranslated region (utr) on gene expression. the 5' utr sequence repressed the reporter activi ... | 2009 | 19618215 |
| the chitinase gene (bbchit1) from beauveria bassiana enhances resistance to cytospora chrysosperma in populus tomentosa carr. | the chinese white poplar (populus tomentosa carr.) is susceptible to infection by plant diseases which severely affect its growth and substantially decrease its economic value. a chitinase gene (bbchit1) from beauveria bassiana was introduced into chinese white poplar (populus tomentosa carr.) by agrobacterium-mediated transformation. the t-dna of plant transformation vector contained the beta-glucuronidase reporter gene (gus) under the control of camv 35s promoter and the neomycin phosphotransf ... | 2010 | 20464449 |
| [cloning and characterization of an aldehyde dehydrogenase gene gmaldh3-1 from soybean]. | to research the mechanism of soybean reproductive development, we identified a number of flower development related genes in soybean by microarray hybridization. a gene predominately expressed in soybean flowers was chosen for further analysis. through bioinformatic and rt-pcr approaches, the full-length gene was cloned from soybean flowers. the results of blast searching indicated that this gene encoded for an aldehyde dehydrogenase and was named as gmaldh3-1. gmaldh3-1 contains a complete open ... | 2010 | 20466639 |
| bidirectionalization of a methyl jasmonate-inducible plant promoter. | a unidirectional promoter can be transformed into a bidirectional module by artificial methods. here we report the bidirectionalization of the methyl jasmonate (meja)-inducible ptdrl02 promoter derived from poplar [(populus tomentosa × p. bolleana) × p. tomentosa] in planta. construction of the bidirectional ptdrl02 promoter (designated as mptdrl02) was rapidly achieved by introducing a minimal 35s promoter in the opposite orientation to the 5' end of ptdrl02. β-glucuronidase (gus) and green flu ... | 2010 | 20953667 |
| enhanced resistance to fungal pathogens in transgenic populus tomentosa carr. by overexpression of an nsltp-like antimicrobial protein gene from motherwort (leonurus japonicus). | the antimicrobial protein gene ljamp2 is a plant non-specific lipid transfer protein from motherwort (leonurus japonicus). in this study, it was introduced into chinese white poplar (populus tomentosa carr.) via agrobacterium-mediated transformation with neomycin phosphotransferase ii gene conferring kanamycin resistance as selectable marker. a total of 16 poplar lines were obtained, and polymerase chain reaction (pcr) analysis established the stable integration of transgenes in the plant genome ... | 2010 | 21084346 |
| isolation of a leafy homolog from populus tomentosa: expression of ptlfy in p. tomentosa floral buds and ptlfy-ir-mediated gene silencing in tobacco (nicotiana tabacum). | to understand the genetic and molecular mechanisms underlying floral development in populus tomentosa, we isolated ptlfy, a leafy homolog, from a p. tomentosa floral bud cdna library. dna gel blot analysis showed that ptlfy is present as a single copy in the genomes of both male and female individuals of p. tomentosa. the genomic copy is composed of three exons and two introns. relative expression levels of ptlfy in tissues of p. tomentosa were estimated by rt-pcr; our results revealed that ptlf ... | 2010 | 21104255 |
| over-expression of the triploid white poplar ptdrl01 gene in tobacco enhances resistance to tobacco mosaic virus. | a full-length cdna, designated as the populus tomentosa disease resistance-like 01 (ptdrl01) gene, was isolated from triploid white poplar [(populus tomentosa × p. bolleana) × p. tomentosa]. the protein thought to be produced by the ptdrl01 gene contains a nuclear localisation sequence (nls), a toll/interleukin-1 receptor (tir) homologue region, a nucleotide binding site (nbs) and a leucine-rich repeat (lrr) domain. the protein also exhibits a considerable degree of homology to n-like resistance ... | 2010 | 21143735 |
| ectopic expression of a poplar apetala3-like gene in tobacco causes early flowering and fast growth. | a mads-box gene, designated ptap3, was isolated from a floral bud cdna library derived from populus tomentosa. analysis by multiple alignments of both nucleotide and amino acid sequences, together with phylogenetic analysis, revealed that ptap3 is an ortholog of arabidopsis ap3. analysis of rna extracts from vegetative and reproductive tissues of p. tomentosa by rt-pcr indicated that ptap3 is expressed in roots, stems, leaves and vegetative and floral buds. notably, the expression of ptap3 fluct ... | 2011 | 21293905 |
| combination of biological pretreatment with liquid hot water pretreatment to enhance enzymatic hydrolysis of populus tomentosa. | a novel stepwise pretreatment of combination of fungal treatment with liquid hot water (lhw) treatment was conducted to enhance the enzymatic hydrolysis of populus tomentosa. the results showed that lignin and cellulose increased with the elevating temperature, while significant amount of hemicellulose was degraded during the lhw pretreatment. a highest hemicellulose removal of 92.33% was observed by combination of lenzites betulina c5617 with lhw treatment at 200°c, which was almost 2 times hig ... | 2011 | 22244900 |
| combined expression of antimicrobial genes (bbchit1 and ljamp2) in transgenic poplar enhances resistance to fungal pathogens. | populus species are susceptible to infection by microbial pathogens that severely affect their growth and substantially decrease their economic value. in this study, two pathogenesis-related protein genes consisting of beauveria bassiana chitinase (bbchit1) and motherwort lipid-transfer protein (ljamp2) were introduced into chinese white poplar (populus tomentosa carr.) via agrobacterium-mediated transformation using the hygromycin (hyg) and neomycin phosphotransferase (nptii) genes as selectabl ... | 2012 | 22971569 |
| bacillus thuringiensis protein transfer between rootstock and scion of grafted poplar. | bacillus thuringiensis (bt) cry1ac protein is a toxin against different leaf-eating lepidopteran insects that attack poplar trees. in the present study, the mode of migration of the bt-cry1ac protein within poplar grafts was investigated. grafting was done using pb29 (transgenic poplar 741 with cry1ac genes), cc71 (transgenic poplar 741 with cry3a genes), non-transgenic poplar 741 and non-transgenic populus tomentosa, either as scion or as rootstock. in order to detect migration of bt-cry1ac pro ... | 2012 | 22372666 |
| identification of glutathione s-transferase genes responding to pathogen infestation in populus tomentosa. | stem blister canker, caused by botryosphaeria dothidea, is becoming the most serious disease of poplar in china. the molecular basis of the poplar in response to stem blister canker is not well understood. to reveal the global transcriptional changes of poplar to infection by b. dothidea, solexa paired-end sequencing of complementary dnas (cdnas) from control (nb) and pathogen-treated samples (wb) was performed, resulting in a total of 339,283 transcripts and 183,881 unigenes. a total of 206,586 ... | 2014 | 24870810 |
| highly efficient crispr/cas9-mediated targeted mutagenesis of multiple genes in populus. | the typeⅱcrispr/cas9 system (clustered regularly interspaced short palindromic repeats /crispr-associated 9) has been widely used in bacteria, yeast, animals and plants as a targeted genome editing technique. in previous work, we have successfully knocked out the endogenous phytoene dehydrogenase (pds) gene in populus tomentosa carr. using this system. to study the effect of target design on the efficiency of crispr/cas9-mediated gene knockout in populus, we analyzed the efficiency of mutagenesi ... | 2015 | 26496757 |
| genome-wide identification and characterization of novel lncrnas in populus under nitrogen deficiency. | long non-coding rnas (lncrnas) have been identified as important regulatory factors of gene expression in eukaryotic species, such as homo sapiens, arabidopsis thaliana, and oryza sativa. however, the systematic identification of potential lncrnas in trees is comparatively rare. in particular, the characteristics, expression, and regulatory roles of lncrnas in trees under nutrient stress remain largely unknown. a genome-wide strategy was used in this investigation to identify and characterize no ... | 2016 | 27138920 |
| physiological and biochemical responses and microscopic structure changes of populus tomentosa carr seedlings to 4-bde exposure. | populus species are very effective in remediation of contaminants. polybrominated diphenyl ethers (pbdes) are commonly used as flame retardants and are known to be persistent environmental pollutants. numerous studies have shown that pbdes are rising in human tissues and biota. 4-monobrominated diphenyl ether (4-bde), one of the less brominated pbdes, was served as a model compound for biodegradation of lower brominated congeners. the present study was designed to clarify the effects of 4-bde st ... | 2015 | 25971809 |
| identification and characterization of the populus sucrose synthase gene family. | in this study, we indentified 15 sucrose synthase (ss) genes in populus and the results of rt-qpcr revealed that their expression patterns were constitutive and partially overlapping but diverse. the release of the most recent populus genomic data in phytozome v9.1 has revealed the largest ss gene family described to date, comprising 15 distinct members. this information will now enable the analysis of transcript expression profiles for those that have not been previously reported. here, we perf ... | 2014 | 24508272 |
| [distribution of fine root biomass of main planting tree species in loess plateau, china]. | the distribution of fine roots of pinus tabuliformis, populus tomentosa, prunus armeniaca, robinia pseudoacacia, hippophae rhamnoides, and caragana korshinskii was investigated by using soil core method and the fine root was defined as root with diameter less than 2 mm. the soil moisture and soil properties were measured. the results showed that in the horizontal direction, the distribution of fine root biomass of p. tabuliformis presented a conic curve, and the fine root biomass of the other sp ... | 2014 | 25345038 |
| antiinflammatory effects of tremulacin, a salicin-related substance isolated from populus tomentosa carr. leaves. | tremulacin was shown to inhibit carrageenan-induced paw edema in rats and croton oil-induced ear edema in mice. it was also found to inhibit peritoneal leucocyte migration in rats and acetic acid-induced writhing responses in mice. experiments with isolated longitudinal muscle strips of sensitized guinea pig ileum showed that tremulacin decreased the biosynthesis of slow reaction substance of anaphylaxis. tremulacin exerted inhibitory effects on leukotriene b4 biosynthesis in intrapleural leucoc ... | 1994 | 23195941 |
| arabidopsis edt1/hdg11 improves drought and salt tolerance in cotton and poplar and increases cotton yield in the field. | drought and salinity are two major environmental factors limiting crop production worldwide. improvement of drought and salt tolerance of crops with transgenic approach is an effective strategy to meet the demand of the ever-growing world population. arabidopsis enhanced drought tolerance1/homeodomain glabrous11 (atedt1/hdg11), a homeodomain-start transcription factor, has been demonstrated to significantly improve drought tolerance in arabidopsis, tobacco, tall fescue and rice. here we report t ... | 2016 | 25879154 |
| [regulation of four typical scenic recreational plantations to stand pm2.5 concentration in beijing, china]. | to study the variation of pm2.5 concentration in scenic recreational plantations and its response to meteorological factors, and to analyze the regulation effect of different stands on pm2.5 concentration, in the summer, autumn and winter of 2013, pm2.5 concentration and relevant meteorological factors in four typical scenic recreational plantations (pinus armandii--ginkgo biloba mixed plantation, populus tomentosa--fraxinus chinensi mixed plantation, populus tomentosa pure plantation, multiple- ... | 2015 | 26915205 |
| molecular cloning and characterization of ptrlar3, a gene encoding leucoanthocyanidin reductase from populus trichocarpa, and its constitutive expression enhances fungal resistance in transgenic plants. | the flavonoid-derived proanthocyanidins (pas) are one class of the major defence phenolics in poplar leaves. transcriptional activation of pa biosynthetic genes, resulting in pa accumulation in leaves, was detected following infection by the fungal marssonina brunnea f.sp. multigermtubi using digital gene expression analysis. in order to study pa biosynthesis and its induction by fungi, a putative leucoanthocyanidin reductase gene, ptrlar3, was isolated from populus trichocarpa. sequence compari ... | 2012 | 22268151 |
| [research on systematic evolution of ginkgo biloba based on chemical composition of wood]. | in the present article, the authors mensurated the infrared spectra of 22 conifer and broadleaf trees such as ginkgo biloba l., cycas revoluta thunb., populus tomentosa carr and so on using ftir, analyzed the difference of a number of absorb peaks in fingerprint area, the characteristic absorb peak position of cellulose and lignin of these trees and their alternation regulations of relative intensity, then discussed the evolutional issue of ginkgo biloba. the results show that ginkgo biloba is d ... | 2009 | 19810520 |