| an outbreak of oropouche virus diease in the vicinity of santarem, para, barzil. | an epidemic of human febrile illness caused by oropouche virus was studied in the village of mojui dos campos, pará state, in february 1975. the major clinical symptoms, fever, chills, headache, myalgia, arthralgia and dizziness persisted for 2 to 7 days. leukopenia was commonly observed. some patients were severely ill but no deaths were attributed to the disease. recurrence of symptoms was reported in several people. 55 cases of recent infection were diagnosed in mojuí dos campos between febru ... | 1976 | 941251 |
| [1st register of an epidemic caused by oropouche virus in the states of maranhão and goiás, brazil]. | the authors describe the occurrence of outbreaks caused by oropouche virus (oro) in the states of maranhão and goiás, brazil in 1988. 36 strains of the virus were obtained from the intracerebral inoculation of the blood of 120 patients into 2-3 day-old infant mice. the illness was characterized by headache, fever, pain in the muscles, joints and back, photophobia, retrobulbar pain, nausea and dizziness. 128 of 197 people examined in porto franco, ma, had hemagglutination-inhibiting antibodies to ... | 1989 | 2516642 |
| laboratory transmission of oropouche virus by culex quinquefasciatus say. | | 1987 | 3607353 |
| [arbovirus diseases in south america and caribbean islands (author's transl)]. | historically, yellow fever has dominated arboviral diseases in south america and the caribbean and sylvatic cases still occur every year (more than 200 in 1979). the dengue, by the very large number of cases occurring in the area during successive epidemics--the most important being the last one in 1977-1978--is a serious problem for public health, an epidemic of hemorrhagic dengue being a constant threat. encephalitides are the third syndrome of arboviral etiology observed in the south american ... | 1981 | 6116149 |
| oropouche virus. i. a review of clinical, epidemiological, and ecological findings. | | 1981 | 6782898 |
| transmission of oropouche virus from man to hamster by the midge culicoides paraensis. | oropouche virus (arbovirus family bunyaviridae, simbu serological group) was experimentally transmitted from man to hamster by the bite of the midge culicoides paraensis. infection rates and transmission rates were determined after the midge had engorged on patients with viremia. the threshold titer necessary to enable infection or transmission by the midges was approximately 5.3 log10 of the median lethal dose of the virus in suckling mice per milliliter of blood. transmission was achieved 6 to ... | 1982 | 6800036 |
| [1st occurrence of outbreaks caused by oropouche virus in the state of amazonas]. | | 1982 | 6818662 |
| [meningitis associated with oropouche virus infections]. | | 1982 | 6818668 |
| oropouche virus. ii. epidemiological observations during an epidemic in santarém, pará, brazil in 1975. | an epidemic of oropouche fever occurred in santarém, pará, brazil in 1975. in the first survey for oropouche antibodies involving a random sample of an entire city, infection rates varied from 0-44%, depending on the specific area within the city. women had higher infection rates than men, but this difference was statistically significant only for persons older than 10 years of age. an analysis of school data showed that pupils in the evening classes had a greater increase in absenteeism during ... | 1981 | 7212162 |
| oropouche virus. iii. entomological observations from three epidemics in pará, brazil, 1975. | urban epidemics of oropouche (oro) fever in three municipalities in pará, brazil were studied in 1975. culicoides paraensis (goeldi) were collected during each of the epidemics and there was a positive correlation, by study areas within the city of santarém, between human seropositivity to oro virus and population densities of c. paraensis and culex quinquefasciatus say. the best numerical correlation was with populations of c. paraensis. the relative absence of other species in the areas of hig ... | 1981 | 7212163 |
| oropouche virus. iv. laboratory transmission by culicoides paraensis. | biological transmission of oropouche (oro) virus by culicoides paraensis (goeldi) has been successfully demonstrated in the laboratory. adult culicoides, collected in an area where oro virus was absent, were infected by feeding on viremic hamsters and then periodically exposed to susceptible hamsters at specific intervals post-infectious blood meal. these c. paraensis were capable of biological transmission of the virus 4-9 days post-feeding on viremic hamsters circulating 6.7-9.9 log10smld50/ml ... | 1981 | 7212164 |
| epidemic oropouche virus disease in northern brazil. | | 1981 | 7326505 |
| [outbreak of oropouche virus fever in serra pelada, municipality of curionópolis, pará, 1994]. | in the final of november 1994, an outbreak of a febrile disease was observed in the serra pelada gold mine (5 degrees 35's: 49 degrees 30'w) in the southeast region of pará state. twenty samples were collected and sent to the laboratory of arbovirus of instituto evandro chagas. the tests showed that the disease was caused by oropouche virus (bunyaviridae, bunyavirus, simbu serological group). between 8-22 december 296 serum samples were taken (54 from febrile patients, 16 paired samples and 242 ... | 1996 | 9011877 |
| oropouche virus transmission in the amazon river basin of peru. | seroepidemiologic studies were conducted to determine the prevalence of oropouche (oro) viral antibody, risk factors, and the incidence of infection among residents of the amazon region of peru. blood samples, as well as demographic, cultural, and medical history data, were collected from residents in a sector of the city of iquitos and in an adjacent rural and three neotropical rain forest communities. blood specimens were obtained approximately one year later from a cohort of the same study su ... | 1997 | 9080872 |
| venezuelan equine encephalitis and oropouche virus infections among peruvian army troops in the amazon region of peru. | an outbreak of a febrile illness characterized by headache, ocular pain, myalgia, and arthralgia occurred during june 1994 among peruvian army troops in northern peru. on june 14-16, 1994, clinical data and blood samples were obtained from eight soldiers with a febrile illness, and from 26 others who had a history of febrile illness during the past three months. a follow-up blood sample was obtained 107 days later from four of the febrile and seven of the afebrile soldiers. serum samples were te ... | 1997 | 9230800 |
| epidemiology of endemic oropouche virus transmission in upper amazonian peru. | a cross-sectional serosurvey of a rural community near iquitos, peru was conducted to determine oropouche (oro) virus antibody prevalence and risk factors for human infection. venous blood samples, and demographic, social, and risk factor data were obtained from people age five years of age and older who lived in the village of santa clara on the nanay river, a tributary of the amazon river. sera were tested for oro viral antibody by an elisa. the specificity of viral antibody reactivity was det ... | 1998 | 9840586 |
| nucleotide sequences and phylogeny of the nucleocapsid gene of oropouche virus. | the nucleotide sequence of the s rna segment of the oropouche (oro) virus prototype strain trvl 9760 was determined and found to be 754 nucleotides in length. in the virion-complementary orientation, the rna contained two overlapping open reading frames of 693 and 273 nucleotides that were predicted to encode proteins of 231 and 91 amino acids, respectively. subsequently, the nucleotide sequences of the nucleocapsid genes of 27 additional oro virus strains, representing a 42 year interval and a ... | 2000 | 10675412 |
| identification of simbu, california and bunyamwera serogroup bunyaviruses by nested rt-pcr. | we describe a reverse transcription-polymerase chain reaction (rt-pcr) with primers that anneal to the 5' and 3' ends and amplify the bunyavirus s rna segments. the rt-pcr was done on the fluids of c6/36 cells infected with each of 21 bunyaviruses. the bunyaviruses studied, with the exception of catu virus, produced amplicons having 700 to 1300 base pairs and probably contained the whole s rna segment sequence. a nested pcr performed with these amplicons distinguished california and most bunyamw ... | 2001 | 11280054 |
| diagnosis of oropouche virus infection using a recombinant nucleocapsid protein-based enzyme immunoassay. | oropouche (oro) virus is an emerging infectious agent that has caused numerous outbreaks of an acute febrile (dengue-like) illness among humans in brazil, peru, and panama. diagnosis of oro virus infection is based mainly on serology. two different antigens, hamster serum antigen (hsa) and vero cell lysate antigen (vcla), are currently used in enzyme immunoassays (eias) in brazil and peru, respectively, to investigate the epidemiology of oro virus infection. both antigens involve use of infectio ... | 2001 | 11427552 |
| jatobal virus is a reassortant containing the small rna of oropouche virus. | jatobal (jat) virus was isolated in 1985 from a carnivore (nasua nasua) in tucuruí, pará state, brazil and was classified as a distinct member of the simbu serogroup of the bunyavirus genus, family bunyaviridae on the basis of neutralization tests. on the basis of nucleotide sequencing, we have found that the small (s) rna of jat virus is very similar (>95% identity) to that of oropouche (oro) virus, in particular, the peruvian genotype of oro virus. in comparison, limited nucleotide sequencing ... | 2001 | 11451484 |
| diagnosis of oropouche virus infection by rt-nested-pcr. | using the rt-pcr with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the s segment of simbu serogroup viruses in a nested pcr it was possible to amplify the oropouche virus (oro) genome from the sera of three patients. these results show that this rt-nested-pcr is a useful tool for rapid diagnosis of oropouche fever infections. | 2002 | 11748670 |
| analysis of oropouche virus l protein amino acid sequence showed the presence of an additional conserved region that could harbour an important role for the polymerase activity. | we described here the complete nucleotide sequence of the l rna segment of oropouche virus (genus orthobunyavirus, family bunyaviridae). we found the l rna segment is 6846 nucleotides long and encodes a putative rna polymerase of 2250 amino acids. phylogenetic analysis showed that oro virus cluster to the orthobunyavirus genus confirming the serological classification. it also showed that bunyamwera and california viruses, from the orthobunyavirus genus, are more closely related to each other th ... | 2003 | 12536293 |
| diversity of biting midges of the genus culicoides latreille (diptera: ceratopogonidae) in the area of the yacyretá dam lake between argentina and paraguay. | the culicoides communities have been analyzed between 1993/1998 in the area influenced by the yacyret dam lake (paran river, argentina-paraguay). adults of culicoides were collected monthly by using cdc light traps exposed for 24 h in 9 sampling sites located at both margins of the river; 21 species were recorded. highest values of species richness were recorded during 1993/1994, being quiteria and corpus the sites with the higest number of species (10 and 11, respectively). the species divers ... | 2003 | 12700857 |
| rapid detection of human pathogenic orthobunyaviruses. | modern detection and identification tools can help to provide answers to urgent questions about the incidence, prevalence, and epidemiology of currently emerging diseases. we developed highly sensitive one-step taqman reverse transcription-pcr assays with sensitivities ranging from 10(4) to 10(1) molecules for 11 human pathogens of the orthobunyaviruses. we compared the performances of these assays on three currently available cyclers (abi-prism 7700, lightcycler, and smartcycler). the assay for ... | 2003 | 12843078 |
| oropouche virus: a new human disease agent from trinidad, west indies. | | 1961 | 13683183 |
| linear amplification followed by single primer polymerase chain reaction to amplify unknown dna fragments: complete nucleotide sequence of oropouche virus m rna segment. | the whole nucleotide sequence of oropouche virus medium (m) rna, orthobunyavirus genus, bunyaviridae family, was obtained using a new genomic amplification method. this method is based on the use of a single and specific primer of high melting temperature in a linear amplification (la), followed by a single primer polymerase chain reaction (lasp-pcr). the lasp-pcr was used to walk along the oropouche m rna completing the sequence in seven successive walks. the amplicons obtained in each walking ... | 2004 | 14656460 |
| biting rates and developmental substrates for biting midges (diptera: ceratopogonidae) in iquitos, peru. | biting midges (diptera: ceratopogonidae) were collected at 16 periurban and rural sites around iquitos, peru, between 17 october 1996 and 26 may 1997. culicoides paraensis (goeldi), the principal vector of oropouche virus, was the most commonly collected species (9,086 flies) with culicoides insinuatus wirth & blanton second (7,229 flies). although both species were collected at all sampling sites (linear (distance surveyed approximately 25 km), c. paraensis dominated at northern collection site ... | 2003 | 14765657 |
| [exanthematous diseases and the first epidemic of dengue to occur in manaus, amazonas state, brazil, during 1998-1999]. | in 1998, the fmt/imt-am foundation implemented the surveillance system to diagnose acute undifferentiated febrile syndromes, with the objective of active and passive surveillance in brazilian western amazonian rainforest to identify and diagnose the etiologic agents of acute fever. the diagnoses were performed using serological tests to detect igm antibodies by elisa (enzyme-linked-immunosorbent assay) cdc/opas or using commercial kits. a total of 8,557 serum samples obtained from patients with ... | 2004 | 15765597 |
| changes in relative species compositions of biting midges (diptera: ceratopogonidae) and an outbreak of oropouche virus in iquitos, peru. | species compositions of culicoides paraensis (goeldi) (diptera: ceratopogonidae), the major vector of oropouche virus to humans in central and south american urban cycles, and culicoides insinuatus ortiz & leon differed along a northeast-to-southwest transect across iquitos, department of loreto, peru. the relative distributions of the species were consistent with patterns of human outbreaks along the amazon river. we resumed collection of biting midges between may 2000 and january 2004 at three ... | 2005 | 16119543 |
| oropouche virus isolation, southeast brazil. | an oropouche virus strain was isolated from a novel host (callithrix sp.) in arinos, minas gerais state, southeastern brazil. the virus was identified by complement fixation test and confirmed by reverse transcription-polymerase chain reaction. phylogenetic analysis identified this strain as a genotype iii isolate previously recognized only in panama. | 2005 | 16318707 |
| in vitro and in vivo studies of the interferon-alpha action on distinct orthobunyavirus. | oropouche, caraparu, guama, guaroa and tacaiuma viruses (orthobunyavirus genus) cause human febrile illnesses and/or encephalitis. to achieve a therapeutical agent to prevent and/or treat these diseases we evaluated the antiviral action of interferon-alpha (ifn-alpha) on these orthobunyaviruses. in vitro results showed that all the studied orthobunyaviruses are susceptible to antiviral action of ifn-alpha, but this susceptibility is limited and dependent on both concentration of drug and treatme ... | 2007 | 17368573 |
| reemergence of oropouche fever, northern brazil. | oropouche fever has reemerged in parauapebas and porto de moz municipalities, pará state, brazil. serologic analysis (immunoglobulin m-elisa) and virus isolation confirmed oropouche virus (orov) in both municipalities. nucleotide sequencing of 2 orov isolates from each location indicated genotypes i (parauapebas) and ii (porto de moz) in brazil. | 2007 | 17553235 |
| oropouche virus entry into hela cells involves clathrin and requires endosomal acidification. | oropouche virus (oro), family bunyaviridae, is the second most frequent cause of arboviral febrile illness in brazil. studies were conducted to understand oro entry in hela cells. chlorpromazine inhibited early steps of oro replication cycle, consistent with entry/uncoating. the data indicate that oro enters hela cells by clathrin-coated vesicles, by a mechanism susceptible to endosomal acidification inhibitors. transmission electron microscopy and immunofluorescence indicated that oro associate ... | 2008 | 18840482 |
| oropouche fever epidemic in northern brazil: epidemiology and molecular characterization of isolates. | oropouche fever virus is an important arbovirus associated with febrile disease that re-emerged in 2006 in several municipalities of pará state, bragantina region, amazon, brazil, 26 years after the last epidemic. | 2009 | 19117799 |
| sporadic oropouche virus infection, acre, brazil. | | 2009 | 19193295 |
| apoptosis induced by oropouche virus infection in hela cells is dependent on virus protein expression. | oropouche (orov) is a single-stranded rna arbovirus of the family bunyaviridae, genus orthobunyavirus, which has caused over half a million cases of febrile illness in brazil in the past 30 years. orov fever has been registered almost exclusively in the amazon region, but global warming, deforestation and redistribution of vectors and animal reservoirs increases the risk of oropouche virus emergence in other areas. orov causes a cytolytical infection in cultured cells with characteristic cytopat ... | 2010 | 20080135 |
| detection of saint louis encephalitis virus in dengue-suspected cases during a dengue 3 outbreak. | arboviruses are frequently associated with outbreaks in humans and represent a serious public health problem. among the brazilian arboviruses, mayaro virus, dengue virus (denv), yellow fever virus, rocio virus, saint louis encephalitis virus (slev), and oropouche virus are responsible for most of human cases. all these arboviruses usually produce undistinguishable acute febrile illness, especially in the acute phase of infection. in this study we investigated the presence of arboviruses in sera ... | 2011 | 20645866 |
| arboviral etiologies of acute febrile illnesses in western south america, 2000-2007. | arthropod-borne viruses (arboviruses) are among the most common agents of human febrile illness worldwide and the most important emerging pathogens, causing multiple notable epidemics of human disease over recent decades. despite the public health relevance, little is know about the geographic distribution, relative impact, and risk factors for arbovirus infection in many regions of the world. our objectives were to describe the arboviruses associated with acute undifferentiated febrile illness ... | 2010 | 20706628 |
| oropouche virus experimental infection in the golden hamster (mesocrisetus auratus). | oropouche virus (orov), of the family bunyaviridae, is the second most frequent arbovirus causing febrile disease in brazil. in spite of this, little is known about pathogenesis of orov infection. this report describes an experimental model of orov in golden hamster (mesocricetus auratus). following subcutaneous inoculation of orov, over 50% of the animals developed disease characterized by lethargy, ruffled fur, shivering, paralysis, and approximately one third died. animals were sacrificed on ... | 2011 | 20727376 |
| molecular epidemiology of oropouche virus, brazil. | oropouche virus (orov) is the causative agent of oropouche fever, an urban febrile arboviral disease widespread in south america, with >30 epidemics reported in brazil and other latin american countries during 1960-2009. to describe the molecular epidemiology of orov, we analyzed the entire n gene sequences (small rna) of 66 strains and 35 partial gn (medium rna) and large rna gene sequences. distinct patterns of orov strain clustered according to n, gn, and large gene sequences, which suggests ... | 2011 | 21529387 |
| rna interference targets arbovirus replication in culicoides cells. | arboviruses are transmitted to vertebrate hosts by biting arthropod vectors such as mosquitoes, ticks, and midges. these viruses replicate in both arthropods and vertebrates and are thus exposed to different antiviral responses in these organisms. rna interference (rnai) is a sequence-specific rna degradation mechanism that has been shown to play a major role in the antiviral response against arboviruses in mosquitoes. culicoides midges are important vectors of arboviruses, known to transmit pat ... | 2012 | 23269795 |
| clinical and virological descriptive study in the 2011 outbreak of dengue in the amazonas, brazil. | dengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito aedes aegypti. in the state of amazonas, brazil during the 2011 outbreak of dengue all the four dengue virus (denv) serotypes circulating simultaneously were observed. the aim of the study was to describe the clinical epidemiology of dengue in manaus, the capital city of the state of the amazonas, where all the four denv serotypes were co-circulating simultaneously. | 2014 | 24978469 |
| human and murine ifit1 proteins do not restrict infection of negative-sense rna viruses of the orthomyxoviridae, bunyaviridae, and filoviridae families. | interferon-induced protein with tetratricopeptide repeats 1 (ifit1) is a host protein with reported cell-intrinsic antiviral activity against several rna viruses. the proposed basis for the activity against negative-sense rna viruses is the binding to exposed 5'-triphosphates (5'-ppp) on the genome of viral rna. however, recent studies reported relatively low binding affinities of ifit1 for 5'-ppp rna, suggesting that ifit1 may not interact efficiently with this moiety under physiological condit ... | 2015 | 26157117 |
| detection of oropouche virus segment s in patients and inculex quinquefasciatus in the state of mato grosso, brazil. | this study aimed to investigate the circulation of orthobunyavirus species in the state of mato grosso (mt) brazil. during a dengue outbreak in 2011/2012, 529 serum samples were collected from patients with acute febrile illness with symptoms for up to five days and 387 pools of female culex quinquefasciatus captured in 2013 were subjected to nested-reverse transcription-polymerase chain reaction for segment s of the simbu serogroup followed by nucleotide sequencing and virus isolation in vero c ... | 0 | 26517653 |
| oropouche virus infection and pathogenesis are restricted by mavs, irf-3, irf-7, and type i interferon signaling pathways in nonmyeloid cells. | oropouche virus (orov) is a member of the orthobunyavirus genus in the bunyaviridae family and a prominent cause of insect-transmitted viral disease in central and south america. despite its clinical relevance, little is known about orov pathogenesis. to define the host defense pathways that control orov infection and disease, we evaluated orov pathogenesis and immune responses in primary cells and mice that were deficient in the rig-i-like receptor signaling pathway (mda5, rig-i, or mavs), down ... | 2015 | 25717109 |
| interferon-regulatory factor 5-dependent signaling restricts orthobunyavirus dissemination to the central nervous system. | interferon (ifn)-regulatory factor 5 (irf-5) is a transcription factor that induces inflammatory responses after engagement and signaling by pattern recognition receptors. to define the role of irf-5 during bunyavirus infection, we evaluated oropouche virus (orov) and la crosse virus (lacv) pathogenesis and immune responses in primary cells and in mice with gene deletions in irf3, irf5, and irf7 or in irf5 alone. deletion of irf3, irf5, and irf7 together resulted in uncontrolled viral replicatio ... | 2015 | 26468541 |
| characterization of viral communities of biting midges and identification of novel thogotovirus species and rhabdovirus genus. | more than two thirds of emerging viruses are of zoonotic origin, and among them rna viruses represent the majority. ceratopogonidae (genus culicoides) are well-known vectors of several viruses responsible for epizooties (bluetongue, epizootic haemorrhagic disease, etc.). they are also vectors of the only known virus infecting humans: the oropouche virus. female midges usually feed on a variety of hosts, leading to possible transmission of emerging viruses from animals to humans. in this context, ... | 2016 | 26978389 |
| generation of recombinant oropouche viruses lacking the nonstructural protein nsm or nss. | oropouche virus (orov) is a midge-borne human pathogen with a geographic distribution in south america. orov was first isolated in 1955, and since then, it has been known to cause recurring outbreaks of a dengue-like illness in the amazonian regions of brazil. orov, however, remains one of the most poorly understood emerging viral zoonoses. here we describe the successful recovery of infectious orov entirely from cdna copies of its genome and generation of orov mutant viruses lacking either the ... | 2015 | 26699638 |
| genetic analysis of members of the species oropouche virus and identification of a novel m segment sequence. | oropouche virus (orov) is a public health threat in south america, and in particular in northern brazil, causing frequent outbreaks of febrile illness. using a combination of deep sequencing and sanger sequencing approaches, we determined the complete genome sequences of eight clinical isolates that were obtained from patient sera during an oropouche fever outbreak in amapa state, northern brazil, in 2009. we also report the complete genome sequences of two orov reassortants isolatd from two mar ... | 2015 | 25735305 |
| identification of oropouche orthobunyavirus in the cerebrospinal fluid of three patients in the amazonas, brazil. | oropouche fever is the second most frequent arboviral infection in brazil, surpassed only by dengue. oropouche virus (orov) causes large and explosive outbreaks of acute febrile illness in cities and villages in the amazon and central-plateau regions. cerebrospinal fluid (csf) samples from 110 meningoencephalitis patients were analyzed. the rna extracted from fluid was submitted to reverse transcription-polymerase chain reaction and sequencing to identify orov. three csf samples showed the prese ... | 0 | 22492162 |
| random amplification and pyrosequencing for identification of novel viral genome sequences. | ssrna viruses have high levels of genomic divergence, which can lead to difficulty in genomic characterization of new viruses using traditional pcr amplification and sequencing methods. in this study, random reverse transcription, anchored random pcr amplification, and high-throughput pyrosequencing were used to identify orthobunyavirus sequences from total rna extracted from viral cultures of acute febrile illness specimens. draft genome sequence for the orthobunyavirus l segment was assembled ... | 0 | 22468136 |
| the variability of the large genomic segment of ťahyňa orthobunyavirus and an all-atom exploration of its anti-viral drug resistance. | ťahyňa virus (tahv), a member of the bunyaviridae family (california complex), is an important but neglected human mosquito-borne pathogen. the virus genome is composed of three segments, i.e., small (s), medium (m), and large (l). previous studies on genetic variability of viruses within the california complex were focused on s and m segments, but the l segment remains relatively unstudied. to assess the genetic variation and the relation to virus phenotype we analyzed the l segment sequences o ... | 2013 | 24090866 |
| culicoides biting midges, arboviruses and public health in europe. | the emergence of multiple strains of bluetongue virus (btv) and the recent discovery of schmallenberg virus (sbv) in europe have highlighted the fact that exotic culicoides-borne arboviruses from remote geographic areas can enter and spread rapidly in this region. this review considers the potential for this phenomenon to impact on human health in europe, by examining evidence of the role of culicoides biting midges in the zoonotic transmission and person-to-person spread of arboviruses worldwid ... | 2013 | 23933421 |
| detection of herpesvirus, enterovirus, and arbovirus infection in patients with suspected central nervous system viral infection in the western brazilian amazon. | acute infections of the central nervous system (cns) can be caused by various pathogens. in this study, the presence of herpesviruses (hhv), enteroviruses (evs), and arboviruses were investigated in csf samples from 165 patients with suspected cns viral infection through polymerase chain reaction (pcr) and reverse transcriptase pcr. the genomes of one or more viral agents were detected in 29.7% (49/165) of the csf samples. evs were predominant (16/49; 32.6%) followed by epstein-barr virus (ebv) ... | 2014 | 24760682 |
| establishment of a minigenome system for oropouche virus reveals the s genome segment to be significantly longer than reported previously. | oropouche virus (orov) is a medically important orthobunyavirus, which causes frequent outbreaks of a febrile illness in the northern parts of brazil. however, despite being the cause of an estimated half a million human infections since its first isolation in trinidad in 1955, details of the molecular biology of this tripartite, negative-sense rna virus remain limited. we have determined the complete nucleotide sequence of the brazilian prototype strain of orov, bean 19991, and found a number o ... | 2015 | 25491420 |
| detection of arboviruses of public health interest in free-living new world primates (sapajus spp.; alouatta caraya) captured in mato grosso do sul, brazil. | a sero-epidemiological survey was undertaken to detect the circulation of arboviruses in free-living non-human primates. | 2014 | 24474008 |
| bibliometric analysis of oropouche research: impact on the surveillance of emerging arboviruses in latin america. | given the emergence and reemergence of viral diseases, particularly in latin america, we would like to provide an analysis of the patterns of research and publication on oropouche virus (orov). we also discuss the implications of recent epidemics in certain areas of south america, and how more clinical and epidemiological information regarding orov is urgently needed. | 2017 | 28357048 |
| vegetation loss and the 2016 oropouche fever outbreak in peru. | oropouche virus causes oropouche fever, an arboviral disease transmitted mainly by midges of the genus culicoides and culex mosquitoes. clinical presentation of oropouche fever in humans includes fever, headache, rash, myalgia, and in rare cases spontaneous bleeding and aseptic meningitis. landscape change has been proposed as a driver of oropouche fever emergence. | 2017 | 28327792 |
| oropouche virus: clinical, epidemiological, and molecular aspects of a neglected orthobunyavirus. | oropouche virus (orov) is an important cause of arboviral illness in latin american countries, more specifically in the amazon region of brazil, venezuela and peru, as well as in other countries such as panama. in the past decades, the clinical, epidemiological, pathological, and molecular aspects of orov have been published and provide the basis for a better understanding of this important human pathogen. here, we describe the milestones in a comprehensive review of orov epidemiology, pathogene ... | 2017 | 28167595 |
| oropouche virus is detected in peripheral blood leukocytes from patients. | oropouche virus (orov) is a frequent cause of arboviral febrile disease in the amazon. the present report describes studies done in two patients, one of them; the first orov human case acquired outside of the amazon, which have revealed for the first time the presence of orov in peripheral blood leukocytes. this novel finding raises important issues regarding pathogenesis of human infections and may offer a new tool, for the rapid diagnosis of this neglected infection. j. med. virol. © 2016 wile ... | 2016 | 27787907 |
| detection of antibodies to oropouche virus in non-human primates in goiânia city, goiás. | arboviruses are associated with human disease, and non-human primates (nhps) are important primary hosts. this study shows the detection of antibodies to oropouche virus (orov) in nhps either living in urban parks or acclimatized at the wild animal screening center, goiânia city. | 2017 | 27384834 |
| isolation of madre de dios virus (orthobunyavirus; bunyaviridae), an oropouche virus species reassortant, from a monkey in venezuela. | oropouche virus (orov), genus orthobunyavirus, family bunyaviridae, is an important cause of human illness in tropical south america. herein, we report the isolation, complete genome sequence, genetic characterization, and phylogenetic analysis of an orov species reassortant, madre de dios virus (mddv), obtained from a sick monkey (cebus olivaceus schomburgk) collected in a forest near atapirire, a small rural village located in anzoategui state, venezuela. mddv is one of a growing number of nat ... | 2016 | 27215299 |
| spread of oropouche virus into the central nervous system in mouse. | oropouche virus (orov) is an important cause of arboviral illness in brazil and other latin american countries, with most cases clinically manifested as acute febrile illness referred to as oropouche fever, including myalgia, headache, arthralgia and malaise. however, orov can also affect the central nervous system (cns) with clinical neurological implications. little is known regarding orov pathogenesis, especially how orov gains access to the cns. in the present study, neonatal balb/c mice wer ... | 2014 | 25310583 |
| experimental infection of suckling mice by subcutaneous inoculation with oropouche virus. | oropouche virus, of the family bunyaviridae, genus orthobunyavirus, serogroup simbu, is an important causative agent of arboviral febrile illness in brazil. an estimated 500,000 cases of oropouche fever have occurred in brazil in the last 30 years, with recorded cases also in panama, peru, suriname and trinidad. we have developed an experimental model of oropouche virus infection in neonatal balb/c mouse by subcutaneous inoculation. the vast majority of infected animals developed disease on the ... | 2012 | 22877689 |
| multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of mayaro, oropouche, and oropouche-like viruses. | we describe a sensitive method for simultaneous detection of oropouche and oropouche-like viruses carrying the oropouche s segment, as well as the mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (rt-qpcr). a chimeric plasmid containing both mayaro and oropouche targets was designed and evaluated for the in vitro production of transcribed rna, which could be easily used as a non-infectious external control. to track false-negative results due t ... | 2017 | 28591313 |
| sensitivity to bst-2 restriction correlates with orthobunyavirus host range. | orthobunyaviruses include several recently emerging viruses of significant medical and veterinary importance. there is currently very limited understanding on what determines the host species range of these pathogens. in this study we discovered that bst-2/tetherin restricts orthobunyavirus replication in a host-specific manner. we show that viruses with human tropism (oropouche virus and la crosse virus) are restricted by sheep bst-2 but not by the human orthologue, while viruses with ruminant ... | 2017 | 28628828 |
| the potential for reassortment between oropouche and schmallenberg orthobunyaviruses. | a number of viruses within the peribunyaviridae family are naturally occurring reassortants, a common phenomenon for segmented viruses. using a minigenome-reporter and virus-like particle (vlp) production assay, we have accessed the potential of oropouche virus (orov), schmallenberg virus (sbv), and other orthobunyaviruses within the simbu serogroup to reassort. we found that the untranslated region (utr) in the medium segment is a potential contributing factor for reassortment by the tested vir ... | 2017 | 28800086 |