| the genus abiotrophia (kawamura et al.) is not monophyletic: proposal of granulicatella gen. nov., granulicatella adiacens comb. nov., granulicatella elegans comb. nov. and granulicatella balaenopterae comb. nov. | the genus abiotrophia currently includes four species, abiotrophia defectiva, abiotrophia adiacens, abiotrophia balaenopterae and abiotrophia elegans. recent 16s rrna gene sequencing studies have demonstrated that the genus is not monophyletic and is in need of taxonomic revision. phylogenetically, the genus abiotrophia consists of two distinct lines, a. defectiva, the type species of the genus, and a robust group consisting of a. adiacens, a. balaenopterae and a. elegans. therefore, it is forma ... | 2000 | 10826824 |
| atopobacter phocae gen. nov., sp. nov., a novel bacterium isolated from common seals. | two strains of a gram-positive, catalase-negative, facultatively anaerobic, rod-shaped bacterium isolated from common seals were characterized using phenotypic and molecular taxonomic methods. the two strains closely resembled each other based on their biochemical characteristics, and page analysis of whole-cell protein patterns confirmed their close phenotypic affinity. 16s rrna gene sequencing showed that the two strains were genetically highly related (99.8% sequence similarity) and that they ... | 2000 | 11034483 |
| antimicrobial susceptibility of abiotrophia adiacens and abiotrophia defectiva. | the susceptibilities of 27 abiotrophia adiacens (proposed reclassification granulicatella adiacens comb.nov., collins & lawson, 2000) and 12 abiotrophia defectiva isolates were tested by microdilution in pyridoxal hydrochloride and lysed horse blood supplemented mueller-hinton broth. according to nccls interpretative criteria for streptococcus spp. not streptococcus pneumoniae, the susceptibilities of a. adiacens and a. defectiva were, respectively: penicillin, 55% and 8%; amoxicillin, 81% and 9 ... | 2000 | 11109021 |
| serological properties of abiotrophia and granulicatella species (nutritionally variant streptococci). | serological variations were examined among 12 type or reference strains and 91 oral isolates of vitamin b6-dependent abiotrophia and granulicatella spp. rabbits were immunized with whole cells of 12 selected strains and 10 typing antisera were obtained, which were unreactive with the lancefield group a to g antigen preparations. the reactivity of the antisera and autoclaved cell surface antigen extracts was tested by double diffusion in agar gel and a capillary precipitin test. these typing anti ... | 2000 | 11220686 |
| granulicatella and abiotrophia species from human clinical specimens. | one hundred one isolates of nutritionally variant streptococci from 97 patients were phenotypically characterized and compared with the type strains of granulicatella adiacens (formerly abiotrophia adiacens) (atcc 49175(t)) abiotrophia defectiva (atcc 49176(t)), and granulicatella elegans (formerly abiotrophia elegans) (dsm 11693(t)). of the isolates, 55 and 43 resembled g. adiacens and a. defectiva, respectively, while 3 strains resembled g. elegans. phenotypic characteristics useful in differe ... | 2001 | 11574566 |
| molecular diagnosis of granulicatella elegans on the cardiac valve of a patient with culture-negative endocarditis. | we herein report one case of culture-negative infectious endocarditis (ie) where the organism, granulicatella elegans, was identified by molecular analysis using broad-range pcr primers complementary to the 16s rrna gene on the removed valve. the results and utility of this method are discussed. | 2002 | 11980974 |
| bacteriologic findings associated with chronic bacterial maxillary sinusitis in adults. | an open-label, multicenter study was performed to assess bacteriologic findings associated with chronic bacterial maxillary sinusitis in adults. seventy aerobic (52.2%) and 64 anaerobic (47.8%) pathogens were recovered from clinically evaluable patients at baseline (before therapy). the most commonly isolated anaerobes were prevotella species (31.1%), anaerobic streptococci (21.9%), and fusobacterium species (15.6%). the aerobes most frequently recovered included streptococcus species (21.4%), h ... | 2002 | 12145727 |
| vertebral osteomyelitis and endocarditis of a pacemaker lead due to granulicatella (abiotrophia) adiacens. | systemic infection due to granulicatella (formerly abiotrophia), a species of nutrition-deficient gram-positive cocci, is rare. we present the case of a 68-year-old diabetic male who presented with back pain and a history of fever and chills. imaging studies revealed vertebral osteomyelitis of the th 10/11 region. transesophageal echocardiography disclosed a vegetation adjacent to the pacemaker lead and blood cultures grew granulicatella adiacens. a diagnosis of vertebral osteomyelitis and endoc ... | 2002 | 12382095 |
| granulicatella adiacens and abiotrophia defectiva bacteraemia characterized by 16s rrna gene sequencing. | traditionally, the identification, epidemiology and spectrum of clinical diseases caused by granulicatella adiacens and abiotrophia defectiva are dependent upon their phenotypic characterization. during a 6-year period (july 1995-june 2001), seven and two alpha-haemolytic streptococci were identified as g. adiacens and a. defectiva, respectively, by 16s rrna gene sequencing. three patients with haematological malignancies and neutropenic fever had primary bacteraemia. three patients with valvula ... | 2003 | 12543919 |
| septic arthritis caused by granulicatella adiacens: diagnosis by inoculation of synovial fluid into blood culture bottles. | granulicatella species, formerly known as nutritionally variant streptococci, cause a variety of infections, primarily endocarditis. we report the first culture-proven case of a granulicatella species causing septic arthritis. a 68-year-old female presented with knee pain and swelling. she was initially evaluated with arthrocentesis and arthroscopy, but no organism was identified. her pain improved after a brief course of antibiotics but recurred 3 months later. she underwent repeat arthrocentes ... | 2003 | 12720043 |
| usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles. | due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ... | 2003 | 12734240 |
| molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses. | chronic maxillary sinusitis is a chronic inflammatory condition in which the role of microbial infection remains undefined. bacteria have been isolated from chronically inflamed sinuses; however, their role in the chronicity of inflammation is unknown. the objective of this study was to determine whether bacteria are present in clinical samples from chronic maxillary sinusitis and to assess the diversity of the flora present. washes and/or tissue samples from endoscopic sinus surgery on 11 patie ... | 2003 | 12808082 |
| a case of endocarditis due to granulicatella adiacens. | | 2003 | 12848740 |
| inhibition of fibronectin binding of some bacterial cells by subtle ph increase within the physiological range. | the fibronectin (fn)-binding ability of microorganisms is considered to be involved in their pathogenicities. granulicatella adiacens, a member of the oral flora and a causative agent of culture-negative infective endocarditis, showed nearly maximum binding to immobilized fn at ph 7.2 but greatly reduced binding at a slightly higher ph 7.4 and almost no binding at ph 7.6 in the presence of physiological concentration of nacl (0.15 m). a similar ph-sensitive fn-binding property was noted with esc ... | 2003 | 14499992 |
| rpob gene sequence-based identification of aerobic gram-positive cocci of the genera streptococcus, enterococcus, gemella, abiotrophia, and granulicatella. | we developed a new molecular tool based on rpob gene (encoding the beta subunit of rna polymerase) sequencing to identify streptococci. we first sequenced the complete rpob gene for streptococcus anginosus, s. equinus, and abiotrophia defectiva. sequences were aligned with these of s. pyogenes, s. agalactiae, and s. pneumoniae available in genbank. using an in-house analysis program (svarap), we identified a 740-bp variable region surrounded by conserved, 20-bp zones and, by using these conserve ... | 2004 | 14766807 |
| "streptococcus milleri" endocarditis caused by streptococcus anginosus. | unlike other viridans streptococci, members of the "streptococcus milleri group" are often associated with abscess formation, but are only rare causes of infective endocarditis. although it has been shown that almost all s. intermedius isolates and most s. constellatus isolates, but only 19% of s. anginosus isolates, were associated with abscess formation, no report has addressed the relative importance of the 3 species of the "s. milleri group" in infective endocarditis. during a 5-year period ... | 2004 | 14972375 |
| central nervous system infections due to abiotrophia and granulicatella species: an emerging challenge? | although abiotrophia and granulicatella species, previously referred to as nutritionally variant streptococci, were initially identified over 40 years ago, isolation of these pathogens from the central nervous system (cns) was first noted only recently. recognition of cns involvement with these organisms is of great concern given the association of abiotrophia/granulicatella infections with increased morbidity and mortality as well as greater bacteriologic failure and relapse rates. we describe ... | 2004 | 15023423 |
| identification and characterization of bacterial-binding property in the type iii repeat domain of fibronectin. | to characterize fibronectin binding with granulicatella adiacens, a causative agent of infective endocarditis, monoclonal antibodies were generated against human fibronectin and selected for their capacity to inhibit the fibronectin binding of the organism. thermolysin and lysyl-endopeptidase digests of fibronectin were characterized by western blot. the epitope of inhibitory monoclonal antibody was found in the central portion of fibronectin known as the cell-binding domain, and not in the n-te ... | 2004 | 15219833 |
| phenotypic and molecular characterization of erythromycin resistance in four isolates of streptococcus-like gram-positive cocci causing bacteremia. | among nine patients with bacteremia caused by granulicatella or gemella in a 6-year period (july 1995 to june 2001), three had bacteremia caused by erythromycin-resistant granulicatella adiacens and one had bacteremia caused by erythromycin-resistant gemella haemolysans. all four isolates possessed mef genes, whereas none possessed ermt, ermtr, or ermb genes. | 2004 | 15243100 |
| antimicrobial susceptibilities of invasive pediatric abiotrophia and granulicatella isolates. | abiotrophia and granulicatella species have been associated with various infections. antimicrobial susceptibility data for these nutritionally variant streptococcus-like organisms, especially for pediatric isolates, are very limited. little is known about the genetic bases of their resistance mechanisms. we report the results of identification to bacterial species level, antimicrobial susceptibility testing, macrolide resistance testing, and detection of genes encoding that resistance for a coll ... | 2004 | 15365035 |
| granulicatella (abiotrophia) adiacens infection associated with a total knee arthroplasty. | we describe the first case of prosthetic joint associated infection due to granulicatella adiacens (formerly abiotrophia adiacens). diagnosis was made by broad spectrum pcr, and later by culture. diagnosis and treatment of this microorganism is difficult. two y after revision and antibiotic treatment, infection was under control but not cured. | 2004 | 15513405 |
| infective endocarditis caused by granulicatella elegans originating in the oral cavity. | we studied the pheno- and genotypes of an oral granulicatella elegans strain in comparison with those of a blood-derived isolate which caused infective endocarditis. the two isolates exhibited identical biochemical characteristics and had the same drug mics. their genotypes were indistinguishable, indicating that these were from the same clone. the transmission of g. elegans from the oral cavity thus should be noted as a possible cause of infective endocarditis. | 2005 | 15750118 |
| the maintenance in the oral cavity of children of tetracycline-resistant bacteria and the genes encoding such resistance. | to investigate the maintenance of tetracycline-resistant oral bacteria and the genes encoding tetracycline resistance in these bacteria in children (aged 4--6 years) over a period of 12 months. | 2005 | 16027144 |
| detection of novel oral species and phylotypes in symptomatic endodontic infections including abscesses. | this study was undertaken to investigate the occurrence of several uncultivated phylotypes and newly named bacterial species in symptomatic endodontic infections. samples taken from cases clinically diagnosed as acute periradicular abscesses or acute periradicular periodontitis were surveyed for the presence of 12 taxa by means of a 16s rrna-gene-based nested or hemi-nested pcr assay. the most prevalent of the target taxa were dialister invisus, olsenella uli, granulicatella adiacens, and synerg ... | 2005 | 16099112 |
| in vitro activity of daptomycin against clinical isolates of gram-positive bacteria. | we determined the activity of daptomycin, a recently fda-approved antimicrobial agent, against clinical isolates of gram-positive bacteria, including viridans group streptococci (16 streptococcus mitis species group, 12 s. mutans species group, 9 s. anginosus species group, 8 s. sanguinis species group, 5 s. salivarius species group) from patients with infective endocarditis, 32 methicillin-resistant staphylococcus aureus, 32 high-level penicillin-resistant streptococcus pneumoniae, 38 vancomyci ... | 2005 | 16133715 |
| prosthetic valve endocarditis from granulicatella adiacens (nutritionally variant streptococci). | nutritionally variant streptococci (nvs) are an unusual but significant cause of endocarditis, with increased morbidity and mortality compared to those caused by enterococci or viridans streptococci. for many reasons, nvs endocarditis has remained very difficult to treat medically with antibiotics, and when the bacteria infect prosthetic valves, surgical intervention has historically been required to effect cure. we report the first case of granulicatella adiacens prosthetic valve endocarditis ( ... | 2004 | 16230190 |
| cultivable oral microbiota of domestic dogs. | bacteria were isolated from the dental plaques of nine dogs and a sample of pooled saliva from five other dogs and were then identified by comparative 16s rrna gene sequencing. among 339 isolates, 84 different phylotypes belonging to 37 genera were identified. approximately half of the phylotypes were identified to the species level, and 28% of these were considered members of the indigenous oral microbiota of humans. the 16s rrna gene sequences of the remaining 44 phylotypes were not represente ... | 2005 | 16272472 |
| defining the normal bacterial flora of the oral cavity. | more than 700 bacterial species or phylotypes, of which over 50% have not been cultivated, have been detected in the oral cavity. our purposes were (i) to utilize culture-independent molecular techniques to extend our knowledge on the breadth of bacterial diversity in the healthy human oral cavity, including not-yet-cultivated bacteria species, and (ii) to determine the site and subject specificity of bacterial colonization. nine sites from five clinically healthy subjects were analyzed. sites i ... | 2005 | 16272510 |
| streptococci dominate the diverse flora within buccal cells. | previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ... | 2005 | 16304448 |
| bloodstream and endovascular infections due to abiotrophia defectiva and granulicatella species. | abiotrophia and granulicatella species, previously referred to as nutritionally variant streptococci (nvs), are significant causative agents of endocarditis and bacteraemia. in this study, we reviewed the clinical manifestations of infections due to a. defectiva and granulicatella species that occurred at our institution between 1998 and 2004. | 2006 | 16426445 |
| molecular characterization of subject-specific oral microflora during initial colonization of enamel. | the initial microbial colonization of tooth surfaces is a repeatable and selective process, with certain bacterial species predominating in the nascent biofilm. characterization of the initial microflora is the first step in understanding interactions among community members that shape ensuing biofilm development. using molecular methods and a retrievable enamel chip model, we characterized the microbial diversity of early dental biofilms in three subjects. a total of 531 16s rrna gene sequences ... | 2006 | 16597990 |
| catonella morbi and granulicatella adiacens: new species in endodontic infections. | this study intended to examine samples from primary endodontic infections for the presence of catonella morbi and granulicatella adiacens, 2 species that have been recently suggested to be involved with infections in other oral sites. | 2006 | 16876072 |
| adherence of abiotrophia defectiva and granulicatella species to fibronectin: is there a link with endovascular infections? | during a 6-year period, we isolated three abiotrophia defectiva, six granulicatella adiacens and two g. 'para-adiacens' strains from clinical specimens. all a. defectiva strains were isolated from immunocompetent patients with endovascular infections, whereas the granulicatella spp. strains were isolated from immunosuppressed patients with primary bacteremia. as the capacity of bacteria to adhere to the host extracellular matrix (ecm) has been implicated in the pathogenesis of endovascular infec ... | 2006 | 17064277 |
| array-based identification of species of the genera abiotrophia, enterococcus, granulicatella, and streptococcus. | some species of enterococci and streptococci are difficult to differentiate by phenotypic traits. the feasibility of using an oligonucleotide array for identification of 11 viridans group streptococci was previously established. the aim of this study was to expand the array to identify species of abiotrophia (1 species), enterococcus (18 species), granulicatella (3 species), and streptococcus (31 species and 6 subspecies). the method consisted of pcr amplification of the ribosomal dna intergenic ... | 2006 | 17065265 |
| [abiotrophia and granulicatella]. | nvs grow in culture media with 0.001% pyridoxal hydrochloride (phc) and 0.1% cysteine hydrochloride (cyshc). these bacteria need the help of other organisms to grow on common solid media showing the effect known as "satellitism". both, satellitism and the pyrrolidonilarilamidase test are the key tests for suspecting the presence of nvs. they can grow as a faint haze on blood agar or chocolate agar prepared with the columbia agar base without adding any other substance. the most frequently docume ... | 2006 | 17152218 |
| new consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria. | the clinical and laboratory standards institute (clsi) recently published a new laboratory guideline for antimicrobial susceptibility testing of infrequently encountered or fastidious bacteria not covered in previous clsi publications. the organisms include aeromonas species, bacillus species, and vibrio species that may cause infections following environmental exposure. fastidious organisms that may cause endocarditis or medical device infections include abiotrophia and granulicatella species; ... | 2007 | 17173232 |
| granulicatella elegans native valve infective endocarditis: case report and review. | granulicatella elegans is a fastidious organism that is rarely implicated as a cause of infective endocarditis. here, we describe a patient with mitral valve prolapse who developed g. elegans endocarditis. the organism was isolated from blood cultures and the patient had mitral valvuloplasty and repair, and completed a course of 6 weeks of intravenous antibiotics with no sequela. | 2007 | 17188448 |
| [endocarditis due to granulicatella adiacens]. | case report: a prosthetic aortic valve was implanted in a 41-year-old patient with connatal aortic stenosis. 14 months later a bacterial endocarditis was diagnosed and treated with ceftriaxone. 6 weeks later he had a relapse. satellite streptococci could be cultivated from three blood cultures, later identified as granulicatella adiacens. the patient was treated with penicillin and gentamicin. review of the literature: in a short review of the literature the most important aspects of pathogenesi ... | 2007 | 17221353 |
| identification of species of abiotrophia, enterococcus, granulicatella and streptococcus by sequence analysis of the ribosomal 16s-23s intergenic spacer region. | the feasibility of sequence analysis of the ribosomal 16s-23s intergenic spacer region (its) was evaluated for identification of 24 species of streptococcus, one species of abiotrophia, 18 species of enterococcus and three species of granulicatella. as genbank currently lacks its sequence entries for many species of these four genera, the its sequences of 38 type strains were first sequenced and submitted to genbank to facilitate species identification of these genera. subsequently, the its sequ ... | 2007 | 17374892 |
| [endocarditis due to granulicatella adiacens in an old patient]. | | 2007 | 17565887 |
| granulicatella elegans bacteraemia in patients with abdominal infections. | bloodstream infections with granulicatella (previous abiotrophia) elegans are rare. a few reported cases were associated with infective endocarditis. three cases of bacteraemia with g. elegans in patients who were operated for acute abdominal diseases are described. abdominal foci should be considered when g. elegans is recovered from blood. | 2007 | 17701726 |
| [bacteremia due to abiotrophia defectiva in a febrile neutropenic pediatric patient]. | the presence of granulicatella spp. in bacteremic episodes of neutropenic patients was recently highlighted whereas abiotrophia defectiva, was only isolated in cases of infectious endocarditis. the aim of this study is to describe a case of a.defectiva bacteremia in a leukemic and febrile (40 degrees c) neutropenic (200 gb/mm3) boy. a.defectiva was only isolated from one of the two processed blood samples. although the patient was undergoing an episode of varicela which could have accounted as t ... | 2007 | 17702254 |
| characterization of the bacteriolytic activity of nutritionally variant streptococci. | the bacteriolytic activity of nutritionally variant streptococci (nvs), fastidious microaerophilic bacteria, which are members of the genera abiotrophia and granulicatella, was characterized in a renaturating sds polyacrylamide gel electrophoresis system. bacteriolytic profiles appeared quite different for the three species of nvs examined. granulicatella adiacens or abiotrophia defectiva each presented at least seven lytic bands, four of which were in common, while the other three were species- ... | 2007 | 17889570 |
| infectious intracranial aneurysms caused by granulicatella adiacens. | infectious intracranial aneurysms (iias) are uncommon but severe complications of bacterial endocarditis. we report a case of iia due to a rare nutritionally variant streptococcus, granulicatella adiacens. the 31-year-old young patient, without any known risk for infective endocarditis had a silent clinical course until rupture of iias occurred. despite antibiotic treatment and surgical clipping of iias, a second episode of more severe intracerebral hemorrhage occurred four months later. mitral ... | 2008 | 17928184 |
| proliferation of mitogen-stimulated human peripheral blood mononuclear cells is inhibited by extracellular arginine deiminase of granulicatella elegans isolated from the human mouth. | granulicatella elegans is a member of normal human oral flora and is thought to be a potent pathogen in endocarditis, especially so-called "culture-negative" endocarditis. to elucidate the pathogenicity of this microorganism in inflammatory diseases, the effect of the extracellular products of this bacteria on human peripheral blood mononuclear cells (pbmcs) was examined. culture supernatants produced by oral isolates of g. elegans strongly inhibited the proliferation of pbmc stimulated by the t ... | 2007 | 17982728 |
| infective endocarditis caused by nutritionally variant streptococci. | infective endocarditis caused by nutritionally variant streptococci (nvs) has a higher rate of complications than endocarditis caused by other streptococci. the bacteriologic failure rate and the mortality rate are high. however, current knowledge on this disease derives from previous patient data from 1987. recent case reports showed successful antibiotic treatment in the absence of surgery. here, we report the clinical outcome of infective endocarditis caused by nvs in our hospital. | 2007 | 18030177 |
| molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis. | culture of bronchoalveolar lavage fluid (balf) is the gold standard for detection of pathogens in the lower airways in cystic fibrosis (cf). however, current culture results do not explain all clinical observations in cf, including negative culture results during pulmonary exacerbation and inflammation in the absence of pathogens. we hypothesize that organisms not routinely identified by culture occur in the cf airway and may contribute to disease. to test this hypothesis we used a culture-indep ... | 2007 | 18077362 |
| a novel agent of peritoneal dialysis-related peritonitis: granulicatella adiacens. | | 2008 | 18178954 |
| granulicatella adiacens breast implant-associated infection. | the 1st reported case of breast implant-associated infection due to granulicatella adiacens, formerly known as nutritionally variant streptococci, streptococcus adiacens, and abiotrophia adiacens is presented. microbiology and previously reported cases of infections by this organism are reviewed. | 2008 | 18206331 |
| bacterial diversity in synovial fluids of patients with tmd determined by cloning and sequencing analysis of the 16s ribosomal rna gene. | the purpose of this study was to examine the presence of bacteria in synovial fluids from patients with temporomandibular joint disorder (tmd) by polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequencing. | 2008 | 18206402 |
| helcococcus ovis, an emerging pathogen in bovine valvular endocarditis. | the initial isolation of helcococcus ovis from a valvular thrombus prompted us to investigate the prevalence of this bacterium in bovine valvular endocarditis. specimens from 55 affected hearts were examined by culture using columbia blood agar and cross streaking the inoculated plate with a staphylococcus aureus strain. as confirmed by 16s rrna gene sequencing, h. ovis was isolated with an unexpectedly high frequency of 33%, predominantly as heavy growth and pure culture. the majority of h. ovi ... | 2008 | 18716228 |
| fluorescence in situ hybridisation (fish) accelerates identification of gram-positive cocci in positive blood cultures. | sepsis is a life-threatening disease with a high mortality rate. rapid identification of blood culture isolates plays a crucial role in adequate antimicrobial therapy in sepsis patients. to accelerate microbiological diagnosis, a comprehensive panel of oligonucleotide probes for fluorescence in situ hybridisation (fish) targeting gram-positive cocci was compiled and evaluated on 428 positive blood culture specimens. by combining genus-specific and species-specific probes, the assay allowed discr ... | 2008 | 18718741 |
| bavariicoccus seileri gen. nov., sp. nov., isolated from the surface and smear water of german red smear soft cheese. | the phylogenetic position and physiological characters of six hitherto-unknown lactic acid bacterial isolates, which form part of the surface microbiota of german red smear soft cheese, are reported. the coccoid cells are aerotolerant, gram-positive, catalase-negative and non-motile. the cell-wall peptidoglycan contains alanine, glutamic acid, lysine and aspartic acid and is of the a4alpha type (l-lys-d-asp). the sequences of the 16s rrna, groel and rpob genes of the six isolates are identical a ... | 2009 | 19622668 |
| abiotrophia/granulicatella tubo-ovarian abscess in an adolescent virginal female. | tubo-ovarian abscess (toa) is a common acute complication of pelvic inflammatory disease (pid). it can also develop as a complication of pelvic or abdominal surgery, malignancy, and intra-abdominal processes such as appendicitis. in premenopausal women, pid is the most common cause of tubo-ovarian abscess. we report a case of tubo-ovarian abscess in a virginal adolescent female with no past surgical history and no known history of appendicitis, inflammatory bowel disease, or cancer. cultures of ... | 2010 | 19682931 |
| analytical limits of three beta-glucosidase-based commercial culture methods used in environmental microbiology, to detect enterococci. | the enzyme-based test methods enterolert, chromocult enterococci agar, and mei agar, used to assess water quality through the detection enterococcus spp., have been compared in terms of their analytical specificity and their ability to detect various enterococcal strains. to achieve this goal, we have tested 110 different non-enterococcal bacterial strains and 101 strains of enterococcus spp. isolated from diverse origins. the results obtained showed that 69 (68.3%), 84 (83.2%), and 89 (88.1%) o ... | 2009 | 19700833 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| defining the healthy "core microbiome" of oral microbial communities. | most studies examining the commensal human oral microbiome are focused on disease or are limited in methodology. in order to diagnose and treat diseases at an early and reversible stage an in-depth definition of health is indispensible. the aim of this study therefore was to define the healthy oral microbiome using recent advances in sequencing technology (454 pyrosequencing). | 2009 | 20003481 |
| development of a novel pcr method to comprehensively analyze salivary bacterial flora and its application to patients with odontogenic infections. | the objective of this study was to develop a novel polymerase chain reaction (pcr) method to comprehensively analyze salivary bacterial flora. | 2010 | 20171911 |
| [acetate accumulation and shift of bacterial community during anaerobic sewage sludge fermentation by ph adjustment]. | to investigate the accumulation mechanism of volatile fatty acids (vfas) the shift of bacterial community and the contribution of syntroph acetogenic bacteria during the anaerobic fermentation of sewage sludge under different ph values. | 2009 | 20222451 |
| isolation of abiotrophia/granulicatella species from a brain abscess in an adult patient without prior history of neurosurgical instrumentation. | | 2010 | 20224502 |
| relationship between oral malodor and the global composition of indigenous bacterial populations in saliva. | oral malodor develops mostly from the metabolic activities of indigenous bacterial populations within the oral cavity, but whether healthy or oral malodor-related patterns of the global bacterial composition exist remains unclear. in this study, the bacterial compositions in the saliva of 240 subjects complaining of oral malodor were divided into groups based on terminal-restriction fragment length polymorphism (t-rflp) profiles using hierarchical cluster analysis, and the patterns of the microb ... | 2010 | 20228112 |
| analysis of oral microbiota in children with dental caries by pcr-dgge and barcoded pyrosequencing. | oral microbiota plays a vital role in maintaining the homeostasis of oral cavity. dental caries are among the most common oral diseases in children and pathogenic bacteria contribute to the development of the disease. however, the overall structure of bacterial communities in the oral cavity from children with dental caries has not been explored deeply heretofore. we used high-throughput barcoded pyrosequencing and pcr-denaturing gradient gel electrophoresis (dgge) to examine bacterial diversity ... | 2010 | 20614117 |
| use of groesl as a target for identification of abiotrophia, granulicatella, and gemella species. | we determined the groesl sequences of three species of nutritionally variant streptococci (abiotrophia defectiva, granulicatella adiacens, and granulicatella elegans) and three gemella species (gemella morbillorum, gemella haemolysans, and gemella sanguinis). the nucleotide sequence similarities between the groes and groel genes of the above genera were 41.7 to 85.9% and 63.7 to 84.3%, respectively. the intraspecies similarities of groesl sequences for the isolates of abiotrophia and granulicate ... | 2010 | 20686088 |
| clonal analysis of the microbiota of severe early childhood caries. | severe early childhood caries is a microbial infection that severely compromises the dentition of young children. the aim of this study was to characterize the microbiota of severe early childhood caries. | 2010 | 20861633 |
| antimicrobial susceptibilities and occurrence of resistance genes in bovine helcococcus ovis isolates. | the aim of the study was to investigate antimicrobial susceptibilities of bovine helcococcus ovis isolates and to detect genes encoding for h. ovis erythromycin and tetracycline resistance. twenty-nine isolates were included and the minimal inhibitory concentrations (mics) of seven antimicrobials were determined using test conditions as given in an approved clsi guideline for the pyridoxal-dependent abiotrophia spp. and granulicatella spp. furthermore, the macrolide resistance phenotype was exam ... | 2010 | 21131144 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms. | the purpose of this study was to design and evaluate fluorescent in situ hybridization (fish) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli and to abiotrophia/granulicatella. | 2011 | 21247450 |
| vertebral osteomyelitis associated with granulicatella adiacens. | granulicatella adiacens is a nutritionally variant streptococci. only 3 cases of vertebral osteomyelitis due to these microorganisms have been reported. we experienced a 73-year-old male who consulted us due to fever and back pain of about 1-month duration. on examination, a presystolic murmur was heard in the apical region. echocardiography showed prolapse of the mitral valve, but no vegetation was observed. mri revealed osteomyelitis of lumbar vertebrae. as g. adiacens was detected in blood cu ... | 2010 | 21319041 |
| native valve endocarditis and femoral embolism due to granulicatella adiacens: a rare case report. | the fastidious gram-positive cocci granulicatella adiacens, previously known as nutritionally variant streptococci (nvs) are unusual but significant cause of endocarditis due to increased mortality and morbidity. difficulties in reaching correct bacteriological identification, increased resistance to beta-lactam antibiotics and macrolides and complicated clinical course have contributed to problems in management of cases of infective endocarditis caused by this bacterium. we present the first in ... | 2010 | 21340307 |
| clinical and microbiological studies of children and adolescents receiving orthodontic treatment. | this case-controlled study examined clinical and microbiological parameters in brazilian children and adolescents receiving orthodontic treatment using fixed orthodontic appliances or removable orthodontic appliances. | 2010 | 21344829 |
| granulicatella adiacens bacteremia in an elderly trauma patient. | abstract background: granulicatella adiacens is a normal commensal of human mucosal surfaces, inhabiting the oral cavity and urogenital and gastrointestinal tracts and only rarely causing disease. these gram-positive cocci can be difficult to identify. case report: we report a first case of bacteremia caused by granulicatella adiacens in an elderly female trauma victim. the infection was diagnosed accurately, and the patient was treated successfully. conclusion: granulicatella adiacens may be a ... | 2011 | 21524203 |
| an unusual causative agent for an orbital abscess: granulicatella adiacens. | we present a patient who developed an orbital abscess secondary to a retained organic (wooden) foreign body after a fall. clinically, he had a right sub brow fistula exuding purulent discharge, four millimetres of proptosis, limitation of ocular motility in all directions of gaze, but no signs of optic neuropathy. preoperative computed tomography and magnetic resonance imaging of the orbits was performed to localise the abscess and to facilitate surgical planning. he underwent exploration, drain ... | 2011 | 21574808 |
| target region selection is a critical determinant of community fingerprints generated by 16s pyrosequencing. | pyrosequencing of 16s rrna genes allows for in-depth characterization of complex microbial communities. although it is known that primer selection can influence the profile of a community generated by sequencing, the extent and severity of this bias on deep-sequencing methodologies is not well elucidated. we tested the hypothesis that the hypervariable region targeted for sequencing and primer degeneracy play important roles in influencing the composition of 16s pyrotag communities. subgingival ... | 2011 | 21738596 |
| [granulicatella adiacens: an underestimated cause of endocarditis]. | we report the case of an old man who was affected by an endocarditis related to granulicatella adiacens, an uncommon bacteria, difficult to isolate and responsible for an important morbidity and mortality. thus, it is mandatory to seek for endocarditis when this germ is demonstrated in order to start quickly an effective antibiotic treatment. inversely, in the presence of unexplained endocarditis, further bacteriological investigations are needed to seek for this life nutrition deficient bacteri ... | 2011 | 21834448 |
| Granulicatella adiacens and early-onset sepsis in neonate. | | 2011 | 22000391 |
| the subgingival microbiota of papillon-lefèvre syndrome. | background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ... | 2011 | 22141356 |
| identification and characterization of a fibronectin-binding protein from granulicatella adiacens. | the interaction of microorganisms with fibronectin plays an important role in infective endocarditis. granulicatella adiacens is a member of the oral microbiota, formerly known as nutritionally variant streptococci, and is often isolated from endocarditis patients. in the present study we identified a surface protein, designated cha, which binds to fibronectin, by a plaque hybridization procedure using the csha sequence as probe, which encodes a fibronectin-binding molecule of streptococcus gord ... | 2011 | 22053963 |
| variations of oral microbiota are associated with pancreatic diseases including pancreatic cancer. | objectivethe associations between oral diseases and increased risk of pancreatic cancer have been reported in several prospective cohort studies. in this study, we measured variations of salivary microbiota and evaluated their potential associations with pancreatic cancer and chronic pancreatitis.methodsthis study was divided into three phases: (1) microbial profiling using the human oral microbe identification microarray to investigate salivary microbiota variation between 10 resectable patient ... | 2011 | 21994333 |
| Acidogenic fermentation of proteinaceous sewage sludge: Effect of pH. | Proteinaceous sewage sludge represents the sludge in which the protein content exceeds the carbohydrate content, accounts for a considerable part of sewage sludge. In this study, volatile fatty acids (VFA) production and synthesis pathway as well as bacterial community in acidogenic fermentation of proteinaceous sewage sludge under different pH conditions were investigated. The results indicated that the alkaline pH improved the solubilization and biodegradation of proteins in the sludge. The so ... | 2011 | 22176743 |
| Comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing. | Bacterial contribution to oral disease has been studied in young children, but there is a lack of data addressing the developmental perspective in edentulous infants. Our primary objectives were to use pyrosequencing to phylogenetically characterize the salivary bacterial microbiome of edentulous infants and to make comparisons against their mothers. Saliva samples were collected from 5 edentulous infants (mean age?=?4.6±1.2 mo old) and their mothers or primary care givers (mean age?=?30.8±9.5 y ... | 2011 | 21853142 |