| transformation of gypsy moth (lymantria dispar) cell lines by infection with glyptapanteles indiensis polydnavirus. | glyptapanteles indiensis, a species of braconid parasitic wasp, infects its host lymantria dispar (gypsy moth) with a polydnavirus (gipdv) to suppress the host immune system during parasitization. here it is shown that gipdv can infect l. dispar cell lines and that a portion of the gipdv genome is stably maintained in infected cells. results of southern hybridization analyses suggested that this portion of the gipdv genome is integrated into the l. dispar cellular genome. this is the first repor ... | 1996 | 8780687 |
| transformation of lepidopteran and coleopteran insect cell lines by glyptapanteles indiensis polydnavirus dna. | recently investigators showed that polydnavirus dna from the parasitic wasp glyptapanteles indiensis could transform gypsy moth l. dispar cell lines in vitro (mckelvey et al., 1996). here we show gipdv dna is capable of transforming in vitro to varying degrees lepidopteran (iplb-tn-r2, iplb-sf-21, ial-pid2, iplb-hvt1) and coleopteran (iplb-du182e) insect cell lines derived from various somatic tissue types. an insect cell line derived from dipteran aedes albopictus (c7/10) could not be transform ... | 1999 | 10475266 |
| evidence for integration of glyptapanteles indiensis polydnavirus dna into the chromosome of lymantria dispar in vitro. | polydnaviruses replicate within calyx cells of the female ovaries of certain species of parasitic wasps and are required for the successful parasitization of lepidopteran hosts. these viruses, which have unusual double-stranded circular dna segmented genomes, are integrated as proviruses into the genomes of their associated wasp hosts and are believed to be transmitted vertically through germline tissue. here, by combined southern hybridization, polymerase chain reaction (pcr) assays and viral s ... | 2000 | 10653915 |
| quantitative expression analysis of a glyptapanteles indiensis polydnavirus protein tyrosine phosphatase gene in its natural lepidopteran host, lymantria dispar. | in the present study, expression of a newly identified glyptapanteles indiensis polydnavirus (gipdv) gene encoding a putative protein tyrosine phosphatase (pdvptp) was monitored in vivo in the parasitized host, l. dispar, using one step rt-pcr. expression levels of the pdvptp transcript were also evaluated in various host tissues at different times post parasitization (pp) using rt quantitative competitive pcr (rt-qcpcr). expression levels varied, with the most abundant transcript detected in ho ... | 2003 | 12752661 |
| morphological and genomic characterization of the polydnavirus associated with the parasitoid wasp glyptapanteles indiensis (hymenoptera: braconidae). | glyptapanteles indiensis polydnavirus (gipdv) is essential for successful parasitization of the larval stage of the lepidopteran lymantria dispar (gypsy moth) by the endoparasitic wasp glyptapanteles indiensis. this virus has not been characterized previously. ultrastructural studies of gipdv showed that virions had a rod-like or rectangular form and each contained as many as ten nucleocapsids enclosed by a single unit membrane envelope. field inversion gel electrophoresis (fige) analysis of the ... | 2003 | 12867635 |
| quantitation of a glyptapanteles indiensis polydnavirus gene expressed in parasitized host, lymantria dispar, by real-time quantitative rt-pcr. | glyptapanteles indiensis is a polydnavirus-carrying wasp that parasitizes early instar gypsy moth larvae. during oviposition, the wasp injects calyx fluid containing polydnavirus along with its eggs into the host. within the host, expression of polydnavirus genes triggers a set of changes in host physiology, which are of critical importance for the survival of the wasp. in the present study, a g. indiensis polydnavirus (gipdv) gene, represented by cdna clone gipdv 1.1, was selected for expressio ... | 2003 | 14625047 |
| characterization and transcriptional analysis of protein tyrosine phosphatase genes and an ankyrin repeat gene of the parasitoid glyptapanteles indiensis polydnavirus in the parasitized host. | glyptapanteles indiensis (braconidae, hymenoptera) is an endoparasitoid of lymantria dispar, the gypsy moth. expression of g. indiensis polydnavirus (gibv)-encoded genes within the pest host results in inhibition of immune response and development and alteration of physiology, enabling successful development of the parasitoid. here, gibv genome segment f (segf), an 18.6 kb segment shown to encode nine protein tyrosine phosphatase (ptp) genes and a single ankyrin repeat gene (ank), is analysed. p ... | 2006 | 16432017 |
| structure and evolution of a proviral locus of glyptapanteles indiensis bracovirus. | bracoviruses (bvs), a group of double-stranded dna viruses with segmented genomes, are mutualistic endosymbionts of parasitoid wasps. virus particles are replication deficient and are produced only by female wasps from proviral sequences integrated into the wasp genome. virus particles are injected along with eggs into caterpillar hosts, where viral gene expression facilitates parasitoid survival and therefore perpetuation of proviral dna. here we describe a 223 kbp region of glyptapanteles indi ... | 2007 | 17594494 |