| purification and some enzymatic properties of the chitosanase from bacillus r-4 which lyses rhizopus cell walls. | a strain of bacillus sp (bacillus r-4) produces a protease and a carbohydrolase both of which have the ability to lyse rhizopus cell walls. of the enzymes, the carbohydrolase has been purified to an ultracentrifugally and electrophoretically homogeneous state, and identified as a chitosanase. the enzyme was active on glycol chitosan as well as chitosan. molecular weight of the purified enzyme was estimated as 31 000 and isoelectric point as ph 8.30. the enzyme was most active at ph 5.6 and at 40 ... | 1975 | 69 |
| soil fungistasis: elevation of the exogenous carbon and nitrogen requirements for spore germination by fungistatic volatiles in soils. | axenic, washed conidia of fusarium solani f. sp. phaseoli, aspergillus flavus, and verticillium albo-atrum were placed on washed difco purified agar discs along with an inorganic salt solution containing various levels of carbon and nitrogen substrates. these discs were exposed to volatiles from six soils (ph 5.1-8.6). fusarium solani macroconidial germination was inhibited mostly by volatiles from soils of ph 5.1, 6.1, 7.0, and 7.5, but high levels of glucose and nh4cl reversed this inhibition, ... | 1975 | 135 |
| isolation and purification of flavobacterium alpha-1,3-glucanase-hydrolyzing, insoluble, sticky glucan of streptococcus mutans. | studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of streptococcus mutans, streptococcus sanguis, and streptococcus sp. and their susceptibilities to dextranases. among the polysaccharides examined, insoluble glucans were rather resistant to available dextranase preparations, and the insoluble, sticky glucan produced by s. mutans omz 176, which could be important in formation of dental plaques, was the most resistant. by enrichment cul ... | 1975 | 370 |
| production, purification, and characterization of an extracellular chitosanase from streptomyces. | the synthesis by streptomyces sp. no. 6 of an extracellular chitosanase was induced by glucosamine. the enzyme was purified to homogeneity by sephadex g-100, carboxymethyl-cellulose, and diethylaminoethyl-cellulose chromatography. the purified enzyme hydrolyzed chitosan (the beta-1,4-linked polymer of glucosamine) but not chitin nor carboxymethyl-cellulose. the only products of the hydrolysis detectable by paper chromatography were di- and triglucosamine. sephadex g-100 chromatography and sodium ... | 1975 | 371 |
| flourescent properties of histidine decarboxylase from micrococcus sp. n. | a dependency of fluorescence parameters of histidinedecarboxylase (hdc) from micrococcuc sp. n. on ph values is studied. native hds has a short-waved maximum position (325 nm) and a small half-width of the fluorescence spectrum (48nm). the change in the quantum yield of the enzyme fluorescence was parallel with the change of the enzymatic activity. triptophane residues of native hdc are located at hydrophobic region of the enzyme globula. the dependency of hdc flourescence parameters on ph value ... | 1975 | 1114 |
| the size ph, and redox potential of the cecum in mice associated with various microbial floras. | cecal size and in situ redox potential and ph of cecal contents were determined in conventionally reared mice and mice reared under a variety of gnotobiotic conditions: germfree, monoassociated with a cecal clostridium sp., hexaflora-associated and thermoduric polyflora-associated. the mean eh was approximately +200 mv in germfree and -200 mv in conventional mice. the eh was close to zero in the monoassociated mice, thus occupying a position intermediate between the germfree and conventional mic ... | 1976 | 2933 |
| 2 new species of microsporidians (protozoa, microsporidia) from mosquitoes of the family chironomidae. | two new species of microsporidians are described from the mosquitoes of the genus chironomus collected in water bodies of the north-eastern ussr. bacillidium chironomi sp. n. injuring the adipose tissue of the fourth stage larvae of chironomus dorsalis mg. has rod-shaped spores 15 (11 to 19) x 0.7 (0.6 to 0.9) mu in size. duboscqia chironomi sp. n. injures the adipose tissue of the fourth stage larvae of chironomus plumosus l. fresh spores of this species are egg-shaped, 6.2 (5.8 to 6.5) x 3.8 ( ... | 1975 | 3757 |
| hepatitis b surface antigen (australia antigen) in mosquitoes collected in senegal, west africa. | during july and august of 1973, 9,198 mosquitoes were collected in the republic of senegal. eight species of mosquitoes were found in the collections: culex thalassius, culex pipiens quinquefasciatus, culex trigripes, culex phillipi, aedes irritans, aedes aegypti, anopheles gambiae, and mansonia sp. specimens were sorted by biological condition; those obviously engorged were designated as (e), females with swollen abdomens not conspicuously blooded were considered gravid (g), and those with norm ... | 1976 | 3983 |
| the microbial metabolism of acetophenone. metabolism of acetophenone and some chloroacetophenones by an arthrobacter species. | 1. an organism that utilizes acetophenone as sole source of carbon and energy was isolated in pure culture and tentatively identified as an arthrobacter sp. 2. cell-free extracts of the acetophenone-grown organism contained an enzyme, acetophenone oxygenase, that catalysed an nadph-dependent consumption of o(2) in the presence of the growth substrate; approx. 1mol of o(2) and 1mol of nadph were consumed per mol of acetophenone oxidized. 3. cell-free extracts also contained an enzyme capable of t ... | 1975 | 4061 |
| properties of a purified nad-linked alpha-glycerophosphate dehydrogenase from leptomonas sp.; activation by polyamines. | | 1976 | 4065 |
| biosynthesis of chloramphenicol in streptomyces sp. 3022a. identification of p-amino-l-phenylalanine as a product from the action of arylamine synthetase on chorismic acid. | products obtained from the action of arylamine synthetase on g-14cchorismic acid were fractionated by gel filtration and ion exchange column chromatography to yield a partially purified radioactive component with an arylamine function. from its ultraviolet absorption spectrum and thin-layer chromatographic behaviour the product was considered to be p-aminophenylalanine and the identification was confirmed by co-crystallization with an authentic specimen. specific deamination of the product with ... | 1976 | 4210 |
| superficial cell-wall layers on spirillum "ordal" and their in vitro reassembly. | the cell envelope of sporillum sp. strain "ordal" (possibly a variety of s. anulus) demonstrated multiple superficial wall layers which were diverse in their macromolecular arrays. negative staining and freeze-etching techniques revealed an outer hexagonally packed layer and an inner tetragonally packed layer. however, both thin sections and freeze-etched cleavages of the wall showed that each of these regular structures rested upon a backing layer, and that there was a delicate amorphous layer ... | 1976 | 4216 |
| the effects of some factors on the growth and morphology of naegleria sp. and three strains of the genus acanthamoeba. | the effects of various biophysical and chemical factors on the cytology of vegetative stages of naegleria sp., vitek strain, acanthamoeba culbertsoni, acanthamoeba castellanii, neff strain and acanthamoeba polyphaga, no. 1289, were studied. the amoebae were cultured in a liquid medium under axenic conditions. the optimum temperature was 37 degrees c for pathogenic strains of naegleria sp. and acanthamoeba culbertsoni and 20 degrees c for a. castellanii. no changes were observed in the growth of ... | 1975 | 4366 |
| antimycin a fermentation. ii. fermentation in aerated-agitated fermenters. | fermentation characteristics, previously studied in shake flasks, were reproduced in aerated-agitated fermenters, using three strains of streptomyces sp. which had been selected for their high antimycin a productivity in shake flasks. fermentation in fermenters was run in three stages. the medium consisted of soy flour, glucose, ammonium sulfate and calcium carbonate; initial ph was 7.2 approximately 7.5, and temperature 25 degrees c. the course of fermentation was then modified to encourage max ... | 1976 | 4419 |
| development of microbodies in the yeast kloeckera growing on methanol. | a number of microbodies appear regularly in methanol-grown yeast cells, but rarely in ethanol- or glucose-grown cells. when one of representative methanol-utilizing yeasts, kloeckera sp.no. 2201 (also known as candida bodinii), was cultured on glucose and then transferred into a methanol medium, microbodies of small size could be observed in 2-h old cells. the number of microbodies per sectioned cell reached five to six after 4 h of cultivation. though the number of microbodies did not change du ... | 1976 | 4436 |
| the subcellular distribution and properties of crithidia sp. hydrolases with particular reference to pyrophosphate and orthophosphate monoester phosphohydrolases. | a cell fractionation scheme was developed for studying the distribution of certain hydrolases, especially phosphohydrolases in a crithidia sp. (trypanosomatidae). whilst between 26-56% of the total cellular hydrolase activities were soluble (probably of flagellar pocket origin), a certain percentage, 5-40%, was sedimentable. a particulate fraction obtained after isopycnic density gradient centrifugation (p = 1.187-1.241), designated fraction fa/fb, was enriched in various acid hydrolases (relati ... | 1976 | 5183 |
| isolation of a moderate halophilic ammonia-oxidizing bacterium, nitrosococcus mobilis nov. sp. | an ammonia-oxidizing bacterium was isolated from a sample of brackish water (north sea, harbour of husum). it is a motile large coccus 1.5-1.7 mum in diameter. the extensive cytomembrane system occurring as flattened vesicles in the peripheral region of the cytoplasm and as intrusions into the center of the cytoplasm is to be emphasized as a characteristic mark of identification. the lithoautotrophically growing bacterium turned out to be an obligate halophile. because of its physiological and m ... | 1976 | 5979 |
| an ammonia-oxidizing bacterium, nitrosovibrio tenuis nov. gen. nov. sp. | an ammonia-oxidizing, autotroph growing, slender, curved rod was isolated from the soil of hawaii. it is well distinguishable from any other nitrifying bacteria thus far described by their morphology. the cells are 1.1-3.0 mum long and 0.3-0.4 mum wide. they are motile by means of 1-4 subpolar to lateral flagella. in contrast to most of the ammonia-oxidizing bacteria the isolated vibrio is void of an extensive cytomembrane system. to categorize this not yet described species we propose to create ... | 1976 | 5980 |
| nitrogenase activity in cultured rhizobium sp. strain 32h1: nutritional and physical considerations. | nutritional and physical conditions affecting nitrogenase activity in the strain of "cowpea" rhizobia, 32h1, were examined using cultures grown on agar medium. arabinose in the basic medium (cs7) could be replaced by ribose, xylose, or glycerol, but mannitol, glucose, sucrose, or galactose only supported low nitrogenase (c2h2 reduction) activity. succinate could be replaced by pyruvate, fumarate, malate, or 2-oxoglutarate, but without any carboxylic acid, nitrogenase activity was low or undetect ... | 1976 | 5982 |
| study of the structure of the histidine decarboxylase of micrococcus sp. n. by the method of circular dichroism. | ring dichroism spectra (rd) of histidine decarboxylase (hdc) from micrococcus sp. n. at the regions of peptide bonds (200-240 nm) and aromatic amino acids (250-300 nm) absorption are studied. the treatment of rd spectra according to methods of greenfield-fasman, saksena-vetlaufer and mayer permits to conclude that at the ph range within 4-8 the content of ordered structures of alpha-helix type comprises 20%, that of beta-structure type-40%, while the rest 40% are represented with polypeptide cha ... | 1976 | 6076 |
| alcohol oxidases of kloeckera sp. and hansenula polymorpha. catalytic properties and subunit structures. | 1. alcohol oxidase (alcohol: oxygen oxidoreductase) of a thermophilic methanol-utilizing yeast, hansenula polymorpha dl-1, was isolated in crystalline form. 2. this alcohol oxidase of h. polymorpha was more stable to heat than was the enzyme of kloeckera sp. this difference in heat stability is compatible with the difference in growth temperatures for both yeasts. 3. the crystalline alcohol oxidases of both yeast oxidized the lower primary alcohols (c-2 to c-4) as well as methanol. the apparent ... | 1976 | 6273 |
| purification of several bacteriolytic enzymes by affinity chromatography on lysozyme-lysate of micrococcus lysodeikticus cell wall coupled with sepharose. | using lysozyme-lysate of micrococcus lysodeikticus cell wall coupled with sepharose, several bacteriolytic enzymes were purified from crude preparations of animal and microbial origin. quail egg-white, human milk and salivary lysozymes ec 3.2.1.17 were adsorbed onto the adsorbent at ph 5-7 and eluted with 2m nacl at ph 10. by means of these treatments, lysozymes were purified 20-250 fold with activity recoveries of 60-80%, and the quail lysozyme thus purified was shown to be discelectrophoretica ... | 1975 | 6436 |
| decreased permeability as a mechanism of resistance to methyl benzimadazol-2-yl carbamate (mbc) in sporobolomyces roseus. | mutants of sporobolomyces roseus resistant to benzimidazole fungicides varied in their responses to 2-(thiazol-4-yl) benzimidazole (thiabendazole, tbz), methyl 1-(butylcarbamoyl)-benzimidazol-2-yl carbamate (benomyl) and methyl benzimidazol-2-yl carbamate (carbendazim, mcb). incorporation of 14c mbc into trichloroacetic acid extracts of the sensitive strain s4 increased during a 2 h incubation period, whereas incorporation into the resistant mutant m55 was unchanged. 14c mbc uptake by s4 cells w ... | 1976 | 6624 |
| the biological cycle of caryophyllaeus brachycollis janiszweska, 1953 (cestoda carophyllaeidae) a parasite of barbus meridionalis risso, 1826 in the south of france. | caryophyllaeus brachycollis janiszweska, 1953 has been found for the first time in france in barbus meridionalis risso, 1926. experimentally, the procercoid develops in tubifex sp. and never in limnodrilus sp. the different possibilities of life cycle of c. brachycollis in europe have been compared. | 1975 | 7113 |
| microbial formation and degradation of dimethylamine. | dimethylamine was formed from trimethylamine in soils of different ph values. the rate of disappearance of the secondary amine from soil was affected by ph and was markedly reduced under anaerobiosis. the accumulation of dimethylamine in cultures of micrococcus sp. provided with trimethylamine depended on the nitrogen sources available to the bacterium but was not greatly influenced by the c-n ratio of the medium. dimethylamine and nitrite accumulated in large amounts at ph 6.0 to 8.0 in culture ... | 1976 | 7191 |
| stabilization of a psychrotrophic pseudomonas protease by calcium against thermal inactivation in milk at ultrahigh temperature. | the heat-stable extracellular protease of pseudomonas sp. (isolate mc60) was investigated. heat resistance of the enzyme in milk at sterilization temperature was dependent on the presence of ca2+. the half-life of the enzyme at ultrahigh temperature (149 c) in skim milk or milk-salts buffer with ca2+ was approximately 7.0 s. treatment of milk with chelators completely removed the heatstabilizing effect of milk. the enzyme was partially purified by ammonium sulfate precipitation and column chroma ... | 1976 | 7196 |
| further evidence for the transmission of mansonella ozzardi by simulium amazonicum in brazil. | the transmission of mansonella ozzardi was studied in two rubber collecting villages on the river purus, state of amazonas, brazil. haematophagous insects were collected from human and bovine baits during the day and night: 687 mansonia amazonensis and 154 culicoides sp. indet. were free from infection with m. ozzardi. the former species is probably not a vector, but the low numbers of culicoides dissected preclude any determination of its vector status. thirty-five (0-99%) of 3530 simulium amaz ... | 1976 | 7215 |
| maintainance of specificity, information, and thermostability in thermophilic bacillus sp. glutamine synthetase. | glutamine synthetase has been purified to homogeneity from b. subtilis (37 degrees) b. stearothermophilus (55 degrees), and b. caldolyticus (75 degrees). those characteristics compared include size (6.0 +/- 0.3 x 10(5) daltons), quaternary structure (12 su) amino acid content, substrate km's and specificity for structural analogs, metal ion activation, number and kind of separate feedback modifier sites, and the complexity of modifier-substrate and modifier-modifier site interactions. although t ... | 1976 | 7467 |
| influence of dilution rate on nad(p) and nad(p)h concentrations and ratios in a pseudomonas sp. grown in continuous culture. | a freshwater pseudomonas sp. was grown in continuous culture under steady-state conditions in l-lactate-, succinate-, glucose- or ammonium-limited media. under carbon limitation, the nad(h) (i.e. nad + nadh) concentration of the organisms increased exponentially from approximately 2 to 7 mumol/g dry wt as the culture dilution rate (d) was decreased from 0.5 to 0.02 h-1. organisms grown at a given d in any of the carbon-limited media possessed very similar levels of nad(h). therefore, under these ... | 1976 | 7637 |
| constitutive synthesis of endopolymethylgalacturonase by the microscopic fungus, penicillium sp. 24p. | | 1976 | 8162 |
| otitis media of infancy and early childhood. a double-blind study of four treatment regimens. | a double-blind, randomized trial of four antimicrobial regimens was conducted in 383 infants and children with acute otitis media. the drugs used were penicillin v, amoxicillin trihydrate, erythromycin estolate, and erythromycin estolate with trisulfapyrimidines. aspiration of middle ear fluid for culture was done before treatment and repeated during treatment if fluid persisted. etiologic bacteria were most commonly pneumococci (31%) or haemophilus sp (22%), and an additional 5% of patients had ... | 1976 | 8980 |
| purification and characterization of tonin. | tonin was purified from rat submaxillary glands by differential centrifugation, ammonium sulphate precipitation, gel filtration on sephadex g150, and by ion-exchange chromatography on deae-cellulose, phospho-cellulose, sp-sephadex c25, and sp-sephadex c50. purified tonin was shown to be homogeneous by analytical electrophoresis and by analytical ultracentrifugation analysis. purified tonin was very stable when stored in buffers of low ph values or when incubated at high temperatures in neutral s ... | 1976 | 9189 |
| time course of free and n4-acetylated sulfapyridine concentrations in the plasma and saliva of man after sulfasalazine (salicylazosulfapyridine) administration: preliminary findings. | the time course of free and n4-acetylated sulfapyridine (sp) concentrations in the saliva and whole plasma was determined in a healthy male volunteer after a single 2.0 g oral dose of salicylazosulfapyridine (sasp) as four-500 mg commercial, uncoated tablets. the mean (+/- s.d.) plasma: saliva concentration ratios for free and acetylated sp was 2.04 (+/- 0.24) and 3.12 (+/-0.43), respectively, and were independent of plasma concentration and saliva ph. the elimination half-lives of sp and n4-ace ... | 1976 | 9675 |
| affinity chromatography of mouse interferon: a modified purification procedure utilizing specifically purified antibodies. | interferon preparations of a high degree of purity were obtained by a one-step procedure using affinity chromatography on specifically purified immunoadsorbent. the procedure consisted of binding interferon harvested from serum-free medium and purified by zn-acetate precipitation and sp-sephadex chromatography to cnbr-activated sepharose 4b (column no. 1). in the next step, antiinterferon globulin was purified by affinity chromatography on column no. 1 with the bound interferon. in this way, ant ... | 1976 | 9822 |
| extracellular nuclease produced by a marine bacterium. ii. purification and properties of extracellular nuclease from a marine vibrio sp. | extracellular nuclease produced by a marine vibrio sp., strain no. 2, was purified by salting out with ammonium sulfate and by chromatography on a deae-cellulose column and twice on a sephadex g-200 column. the nuclease was eluted as a single peak in which the deoxyribonuclease (dnase) activity and ribonuclease (rnase) activity appeared together. polyacrylamide disc gel electrophoresis showed a single band of stained protein which had both dnase and rnase activity. the molecular weight of the en ... | 1976 | 10061 |
| thermothrix thioparus gen. et sp. nov. a facultatively anaerobic facultative chemolithotroph living at neutral ph and high temperature. | thermothrix thioparus gen. et ep. nov. occurs naturally in a new mexico hot spring at a temperature of 74 degrees c, a ph of 7.0, and a hs- concentration of 1 mg/litre. the organism is gram-negative, non-motile, 0.5-1.0 x 3-20 mum, and forms cell chains up to 1 cm in length. the resulting filaments do not possess a sheath. sulfur is deposited extracellularly. the organism was isolated using an autotrophic medium with hs- as the energy source and no3- as the terminal electron acceptor. anaerobica ... | 1976 | 10063 |
| roles of low ph, carbon and inorganic nitrogen source use in chlamydospore formation by fusarium solani. | citrate and malate were poorer sources of exogenous carbon than several hexose, pentose, or disaccharide sugars for supporting macroconidial germination by fusarium solani at high conidial density (1 x 10(5) condia/ml). only citrate, however, failed to block chlamydospore morphogenesis to a degree comparable to glucose or other readily used sugars. mostly immature chlamydospores were formed in the presence of citrate. at low conidial density (5 x 10(3) conidia/ml), exogenous carbon-independent m ... | 1976 | 10071 |
| the structure and function of acid proteases. v. comparative studies on the specific inhibition of acid proteases by diazoacetyl-dl-norleucine methyl ester, 1,2-epoxy-3-(p-nitrophenoxy) propane and pepstatin. | comparative studies have been made on the effects of diazoacetyl-dl-norleucine methyl ester (dan), 1,2-epoxy-3-(p-nitrophenoxy)propane (epnp) and pepstatin on acid proteases, including those from acrocylindrium sp., aspergillus niger, aspergillus saitoi, mucor pusillus, paecilomyces varioti, rhizopus chinensis, and trametes sanguinea, and also porcine pepsin ec 3.4.23.1 and calf rennin ec 3.4.23.4 for comparative purposes. these enzymes were rapidly inactivated at similar rates and in 1:1 stioch ... | 1976 | 10290 |
| growth and toxin production by clostridium botulinum in moldy tomato juice. | tomato juice inoculated with cladosporium sp. or penicillium sp. developed ph gradients with the upper portions near the mold mats having ph values near neutrality and the lower portions remaining more acid. clostridium botulinum spores in these moldy tomato juices germinated, grew out, and produced toxin. | 1976 | 10844 |
| serum bactericidal activity in the horseshoe crab, limulus polyphemus. | serum from the horseshoe crab, limulus polyphemus, was examined for bactericidal activity against five species of bacteria. greatest activity was found against pseudomonas putida and flavobacterium sp.; with the former, serum dilutions as high as 1:20 were capable of reducing viable counts by 50% within 2 h. bactericidal activity of a significantly lesser magnitude was demonstrated against serratia marcesencs and salmonella minnesota. no killing was seen when the lobster pathogen aerococcus viri ... | 1976 | 11188 |
| changes in the blood pictureof the red goat of maradi as a function of its gastrointestinal parasitism. | numerous "chèvres de maradi" are bred in republique du niger (cap. niamey), 2 - 10(+6) numbered in 1973. this rustic ruminant is often very parasitized by intestinal nematodes and sporozoa. the most frequent genera are bunostomum (55%), trichostrongylus (40%), strongyloides (27%), oesophagostomum and haemonchus (20%), sometimes moniezia or stilesia, coccidiosis being endemic and very pathogenic, eimeria (70%). polyparasitism is a "modus vivendi" between the host and these various parasites. all ... | 1976 | 11658 |
| the role of o2-limitation in control of nitrogenase in continuous cultures of rhizobrium sp. | | 1976 | 11804 |
| effects of benzodiazepines and pentobarbital on the evoked potentials in the cat brain. | the sites of action of benzodiazepines, diazepam and id-540 7-chloro-5-(o-fluorophenyl)-1-methyl-1,3-dihydro-2h-1,4-benzodiazepin-2-one) on the central nervous system were examined and compared with those of pentobarbital using evoked potentials recorded on the limbic system and hypothalamus in the cat brain. benzodiazepines affected the various neuronal connections of the intra-limbic, limbic-hypothalamic and midbrain-limbic systems; especially the amygdala (amyg)-, ventromedial hypothalamus (v ... | 1976 | 12387 |
| production of an extracellular maltase by thermophilic bacillus sp. kp 1035. | production of extracellular maltase was studied with thermophilic bacillus sp. kp 1035, which was selected as the organism producing the highest levels of maltase. the final enzyme yield was increased by maltose, peptone, and yeast extract but reduced by succinate and fumarate. maximum enzyme production was achieved at 55 degrees c and at an initial ph of 6.2 to 7.0 on a medium containing 0.3% maltose, 1% peptone, 0.1% meat extract, 0.3% yeast extract, 0.3% kh2po4, and 0.1% kh2po4. maltase was s ... | 1976 | 12718 |
| the occurrence of an intraerythrocytic microorganism neitziella rezendei gen. nov. sp. nov. (microtatobiotes, rickettsiales) of poultry in brazil. | an extremely polymorphous intraerythrocytic microorganism occurring in naturally infected turkeys, ducks and fowls has been discovered in the states of minas gerais, rio de janeiro and espirito santo. 2. studies on the morphology and biology have shown that it is a hitherto undescribed heteroxenous parasite for which the name neitziella rezendei gen. nov., sp. nov. is proposed. 3. its morphological features permit its inclusion in the class microtatobiotes and order rickettsiales. 4. microscopic ... | 1976 | 13631 |
| effect of autonomic blockade on the hemodynamic findings in acute cardiac tamponade. | twenty-three closed-chest, alpha-chloralose-anesthetized, volume-expanded, alpha- and beta-adrenergic-blockaded dogs with rate fixed by atrial pacing had 30-90 ml of saline at 37 degrees c infused into the pericardial sac a) with vagus intact, b) after vagotomy, and c) with vagus intact but with systolic pressure augmented with a balloon. a significant reduction in left ventricular (lv) systolic pressure (sp), and cardiac output (co) occurred at a pericardial volume of 30-60 ml, when lv end-dias ... | 1977 | 13662 |
| production of schardinger beta-dextrin by soluble and immobilized cyclodextrin glycosyltransferase of an alkalophilic bacillus sp. | succinylated cyclodextrin glycosyltransferase (ec 3.2.1.19) of an alkalophilic bacillus sp. was adsorbed on a vinylpyridine copolymer. the enzyme had about 25% of the activity of soluble enzyme added. no increase of ph or thermal stability of the enzyme was observed by the adsorption, whereas optimum temperature for the enzyme action was shifted from 50 to 55 degrees c. the enzyme converted starch to cyclodextrine without significant loss of activity under the conditions of 4 times reusing of 6 ... | 1977 | 14746 |
| two distinct types of trimethoprim-resistant dihydrofolate reductase specified by r-plasmids of different compatibility groups. | r-plasmids from a number of trimethoprim-resistant escherichia coli and citrobacter sp. were studied after transfer to e. coli k12 hosts. each was found to specify a dihydrofolate reductase which was resistant to trimethoprim and methotrexate, and which could be completely separated from the host chromosomal enzyme by gel filtration. two distinct types of r-plasmid dihydrofolate reductases were identified. type i enzymes, typified by the r483 enzyme previously described (sköld, o., and widh, a. ... | 1977 | 14961 |
| effects of graft-versus-host reaction on inhibition of tumor growth in vivo and on tumor cytotoxicity in vitro. | in da x wistar f1 rats, growth of 10(4) wistar-specific sp 1 carcinoma cells s.c. was commonly prevented by a mild subclinical graft=versus-host reaction produced by injecting 50 x 10(6) wistar spleen cells i.p. either concurrently with the tumor or 7 to 14 days previously, spleen cells alone had no effect on established tumor, but their injection on day 14 significantly reduced the recurrence rate after excision of tumor on day 21. in vitro tests in tumor-bearing rats with graft-versus-host rea ... | 1977 | 15723 |
| flocculation of influenza virus by a neuraminidase inhibitor, neuraminin, produced by streptomyces sp. | | 1977 | 16382 |
| comparative study of the properties of lipase of microbial origin. | a comparative study of properties of lipases of microbial origin has shown that the ph optimum of the enzymes from geotrichum asteroides and penicillium sp. is at ph 7.5 and 5.0, respectively. lipase from geotrichum asteroides appears more resistant to high temperatures and ph changes than the enzyme from penicillium sp. both enzymes split selectively vegetable oils, especially cotton and sunflower oils. na cholate and deoxycholate at a concentration of 0.05-0.4% act as hydrolysis activators, pa ... | 1976 | 17110 |
| effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and ph. | fungi (rhizopus stolonifer, trichoderma viride, fusarium oxysporum f. sp. conglutinans, cunninghamella echinulata, and several species of aspergillus and penicillium) tolerated higher concentrations of cadmium (cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects of cd. in soil amended with clay minerals, montmorillonite provided partial or total protection against fungistatic effects of cd, whereas additions of kaolinite provided little or ... | 1977 | 18085 |
| purification and some properties of cyclohexylamine oxidase from a pseudomonas sp. | cyclohexylamine oxidase was purified 90-fold from cell-free extracts of pseudomonas sp. capable of assimilating sodium cyclamate. the purified enzyme was homogeneous in disc electrophoresis, and the molecular weight was found to be approximately 80,000 by gel filtration. the enzyme catalyzed the following reaction: cyclohexylamine+o2+h2o leads to cyclohexanone+nh3+h2o2. the enzyme thus can be classified as an amine oxidase; it utilized oxygen as the ultimate electron acceptor. the ph optimum of ... | 1977 | 18451 |
| analysis of antibody response and immunoglobulins in sera of rabbits and guinea pigs immunized with coxiella burnetii. | rabbit and guinea pig sera and their immunoglobulin fractions (igm and igg) were examined by complement-fixation (cf), microagglutination (ma), opsonization-phagocytosis (op) and serum protection (sp) tests at intervals after immunization with live phase i and phase ii coxiella burnetii suspensions. in general, ma antibodies, but also decreased, earlier than cf antibodies. the anamnestic immune response was higher with lower primary doses. both phase ii and phase i cf and ma antibodies as well a ... | 1977 | 18924 |
| fortimicins a and b, new aminoglycoside antibiotics. ii. isolation, physico-chemical and chromatographic properties. | the aminoglycoside antibiotics fortimicins a and b produced by a naturally occurring strain micromonospora sp. mk-70 were isolated from its fermentation beer. fortimicins a and b were isolated as water-soluble, basic, white amorphous powders having molecular formula c17h35n5o6 and c15h32n4o5, respectively. acid hydrolysis of fortimicin a indicated that it has one mole of glycine in its molecule while fortimicin b has not. paper chromatography, silica-gel and carbon thin-layer chromatography reve ... | 1977 | 19408 |
| studies on sterol-ester hydrolase from fusarium oxysporum. i. partial purification and properties. | 1. a search for a long chain fatty acyl sterol-ester hydrolase in microorganisms led to the isolation from soil of five strains belonging to fusarium sp. which produced strong activity in the culture medium. 2. the cholesterol esterase from fusarium oxysporum igh-2 was purified about 270-fold by means of cacl2 precipitation and sephadex g-75 column chromatography. 3. the cholesterol esterase was activated by adekatol and triton x-100. it was inhibited by lecithin and lysolecithin, and completely ... | 1977 | 19426 |
| the catabolism of l-tyrosine by an arthrobacter sp. | an arthrobacter sp. metabolizes l-tyrosine by a pathway involving 3,4-dihydroxyphenylacetate as a key intermediate. p-hydroxyphenylpyruvate is formed from tyrosine by an amino-transferase specifically requiring alpha-ketoglutarate for activity, and is then converted to p-hydroxyphenylacetate by an oxidative decarboxylation. p-hydroxyphenylacetaldehyde is not an intermediate in the formation of p-hydroxyphenylacetate. extracts of the bacterium oxidize 3,4-dihydroxyphenylacetate to delta-carboxyme ... | 1977 | 20216 |
| chromatographic separations of alphavirus strains by hydroxylapatite. | hydroxylapatite column chromatography methods were developed to characterize selected alphavirus populations. different conditions of ph and phosphate molarity were required to obtain satisfactory elution profiles and separations for western equine encephalomyelitis virus strains, compared with eastern equine encephalomyelitis virus and semliki forest virus strains. raising the ph of the buffers effected earlier elutions of all viruses. selection of phosphate gradients with more gentle slopes an ... | 1977 | 20452 |
| nitrogen and ammonia assimilation in the cyanobacteria: purification of glutamine synthetase from anabaena sp. strain ca. | glutamine synthetase was purified from the cyanobacterium anabaena sp. strain ca, a newly isolated marine organism. this organism grows rapidly under nitrogen-fixing conditions and therefore is ideally suited for studies concerning cyanobacterial nitrogen metabolism. studies were conducted to optimize the production of glutamine synthetase by anabaena ca. the highest specific activities were obtained from cells grown in the presence of atmospheric n(2) or kno(3) (13 mm); when nh(4)cl was used as ... | 1977 | 21167 |
| microbial metabolism of aromatic nitriles. enzymology of c-n cleavage by nocardia sp. (rhodochrous group) n.c.i.b. 11216. | 1. an organism utilizing benzonitrile as sole carbon and nitrogen source was isolated by the enrichment-culture technique and identified as a nocardia sp. of the rhodochrous group. 2. respiration studies indicate that nitrile degradation proceeds through benzoic acid and catechol. 3. cell-free extracts of benzonitrile-grown cells contain an enzyme that catalyses the conversion of benzonitrile directly into benzoic acid without intermediate formation of benzamide. 4. this nitrilase enzyme was pur ... | 1977 | 21655 |
| immobilization of yeast microbodies by inclusion with photo-crosslinkable resins. | yeast microbodies containing fad-dependent alcohol oxidase, catalase and d-amino acid oxidase were isolated from methanol-grown cells of kloeckera sp. 2201 and immobilized intact in matrices formed by a short-time illumination of photo-crosslinkable resin oligomers. the relative activities of catalase, alcohol oxidase and d-amino acid oxidase of the gel-entrapped microbodies were 36, 76 and 31% respectively as compared with those of free microbodies. immobilization enhance d the stability of cat ... | 1977 | 21791 |
| localization of substance p-like immunoreactivity in nerves in the tooth pulp. | the occurrence of substance p (sp)-like immunoreactivity was studied in dental pulps of the cat. in untreated animals sp-positive fibres were found in all areas of the pulp. most fibres were seen in central parts of the pulp but they were also observed in relation to the odontoblasts. single, possibly unmyelinated, or fine caliber fibres or small bundles of them were seen running close to large non-fluorescent myelinated nerves, to blood vessels or without any obvious association with either of ... | 1977 | 23511 |
| mycetoma in somalia - results of a survey done from 1959 to 1964. | the pasteur institute studied 103 mycetoma patients in somalia between 1959 and 1964. grains were seen in 94 of them and this, added to cultural features, allowed the diagnosis of 60 pathogens as follows: 44 madurella mycetomi, 1 leptosphaeria senegalensis, 7 pyrenochaeta romeroi (or madurella grisea), 3 allescheria boydii, 1 fusarium sp., 3 neotestudina (zopfia) rosatii, and 1 unidentified; 34 were actinomycetes: 24 streptomyces somaliensis, 4 actinomadura madurae, 3 a. pelletieri and 3 nocardi ... | 1977 | 23657 |
| glucose-6-phosphate dehydrogenase of anabaena sp. kinetic and molecular properties. | the kinetic and molecular properties of cyanobacterial glucose-6-phosphate dehydrogenase, partly purified from anabaena sp. atcc 27893, show that it undergoes relatively slow, reversible transitions between different aggregation states which differ in catalytic activity. sucrose gradient centrifugation and polyacrylamide gel electrophoresis reveal three pincipal forms, with approximate molecular weights of 120 000 (m1), 240 000 (m2) and 345 000 (m3). the relative catalytic activities are: m1 les ... | 1978 | 23737 |
| methylamine dehydrogenase of pseudomonas sp. j. purification and properties. | methylamine dehydrogenase was purified in a homogeneous form from methylamine-grown pseudomonas sp. j. the specific activity of the purified enzyme was 5.19 at 19 degrees c. the molecular weight was estimated to be 105 000, and the enzyme was composed of two kinds of subunit with molecular weights of 40 000 and 13 000, respectively. the enzyme contained little phosphorus, iron and copper. the enzyme had absorption maxima at 278, 330, 430 and 460 nm (shoulder). on addition of methylamine, the pea ... | 1978 | 23836 |
| methylamine dehydrogenase of pseudomonase sp. j isolation and properties of the subunits. | two kinds of subunits, light subunit (mr =1300) and heavy subunit (mr=40 000), were isolated from a methylamine dehydrogenase (mr=105 000) of pseudomonas sp. j. the isolation of the subunits was carried out by gel chromatography after the enzyme had been treated with 3m guanidine-hcl. coexistence of both of the subunit exhibited an absorption maximum only at 278 nm but in addition to the peak at 278 nm. the results indicate that the prosthetic group, assumed to be a derivative of pyridoxal, was ... | 1978 | 23837 |
| activity of nitrogenase and glutamine synthetase in relation to availability of oxygen in continuous cultures of a strain of cowpea rhizobium sp. supplied with excess ammonium. | in samples from nitrogen-fixing continuous cultures of strain cb756 of the cowpea type rhizobia (rhizobium sp.), newly fixed nh+4 is in equiblibrium with the medium, from where it is assimilated by the glutamine synthetase/glutamate synthase pathway. in samples from steady state cultures with different degrees of oxygen-limitation, nitrogenase activity was positively correlated with the biosynthetic of glutamine synthetase in cell free extracts. also, activities in biosynthetic assays were posit ... | 1978 | 23859 |
| microbiological transformation of carbohydrates. glucose isomerization into fructose by washed cells of streptomyces sp. strain 29. | glucose-fructose transformation by washed and packed cells of streptomyces sp. 29 was studied. the cells grown on the nutrient medium containing xylose and co and mg salts were capable to perform glucose-fructose isomerization. the cellular activity depended in a great degree on the temperature, ph and initial glucose concentration; to a lesser extent on co and mg ions present in the incubation mixture, and did not depend on the age of the culture (within 8-69 hours). the activity reached its ma ... | 1978 | 24842 |
| bacteriolytic enzyme induced from pyocinogenic pseudomonas aeruginosa. purification and characterization of pr1-lysozyme. | a bacteriolytic enzyme, pr1-lysozyme, has been purified from the lysate of mitomycin c-induced pyocinogenic pseudomonas aeruginosa, by acrinol treatment, amberlite cg-50 chromatography, ammonium sulfate fractionation, sephadex g-100 gel filtration and two cycles of sp-sephadex c-50 chromatography. homogeneity of the preparation was demonstrated by three electrophoretic techniques. pr1-lysozyme is a basic protein (pi, 9.4) and consists of a single polypeptide chain having a molecular weight of 24 ... | 1978 | 25269 |
| purification and characterization of a secondary alcohol dehydrogenase from a pseudomonad. | growth of pseudomonas sp. nrrl b3266 in the presence of oleic acid resulted in the induction of two enzymes: oleate hydratase, which produced 10(r)hydroxyoctadecanoate, and hydroxyoctadecanoate dehydrogenase, which catalyzed the oxidized nicotinamide adenine dinucleotide-dependent production of 10-oxooctadecanoate. this latter enzyme was purified to homogeneity and shown to consist of two polypeptide chains of about 29,000 daltons each. the enzyme had a broad substrate specificity, catalyzing th ... | 1978 | 25883 |
| anaerobic utilization of phosphite and hypophosphite by bacillus sp. | a bacillus sp. capable of utilizing phosphite and hypophosphite under anaerobic conditions was isolated from cape canerval soil samples. the organism was isolated on a glucose-mineral salts medium with phosphate deleted. anaerobic cultivation of this isolate resulted in decreases in the hypophosphite or phosphite concentration, increases in turbidity, cell count, and dry-cell weight, and decreases in ph and glucose concentration. the optimum hypophosphite concentration for this isolate was 60 mi ... | 1978 | 26310 |
| spin-label studies on the sulfhydryl environment in bovine plasma albumin. 2. the neutral transition and the a isomer. | since we were able to demonstrate that the sulfhydryl group is located in the crevice which opens during the n--f transition (cornell, c. n., & kaplan, l. j. (1978) biochemistry 17 (preceding paper in this issue), the investigation was extended by characterizing the environment during the n--b transition and in the a isomer. the results indicate that the n--b and n--f transitions are very similar in that the sulfhydryl group moves from a restricted to unhindered environment during both. the use ... | 1978 | 26388 |
| carboxypeptidase displaying differential velocity in hydrolysis of methotrexate, 5-methyltetrahydrofolic acid, and leucovorin. | an enzyme that catalyzes the hydrolysis of folic acid and the antifolate methotrexate nearly 20 times more rapidly than the hydrolysis of 5-methyltetrahydrofolate was extraced from a gram-negative bacterium tentatively identified as a flavobacterium sp. the enzyme was purified 500-fold and found to have a molecular weight of about 53,000. apparently a metallo-enzyme, it is inhibited by citrate and ethylenediaminetetraacetic acid (edta). ca2+, co2+, mg2+, and zn2+ reverse inhibition by edta, wher ... | 1978 | 26657 |
| onchocerca sweetae (nematoda: filarioidea): notes on the intermediate host. | microfilariae of onchocerca sweetae are broadly distributed in the superficial layers of the dermis of the water buffalo (bubalus bubalis). a total of 2855 insects representing 20 species were collected from o. sweetae-infected bait buffaloes. only one species, culicoides sp. "m", ingested microfilariae from buffalo skin. larval development of o. sweetae was observed in the thorax of this species. atotal of 829 insects, representing 7 species and including 749 parous culicoides spp. were collect ... | 1978 | 26709 |
| investigation of possible interactions between substance p and transmitter mechanisms in the substantia nigra and corpus striatum of the rat. | the effect of substnace p (sp) on the uptake and release of radiolabelled dopamine (3h-da), 5-hydroxytryptamine (3h-5-ht) and y-aminobutyric acid (3h-gaba) was studied in slices of rat substantia nigra and corpus triatum. sp, 10(-9) to 10(-5) m, failed to modify the uptakes of these compounds during incubations (10-90 min) with slices of either brain region. sp, 10(-6)m, had no apparent effect on the spontaneous output of any of these compounds in either substantia nigra or corpus striatum. in t ... | 1978 | 26766 |
| effect of the composition of the medium and the conditions of aspergillus foetidus cultivation on the biosynthesis of glucoamylase. | the optimal conditions for biosynthesis of exocellular glucoamylase were found in the course of submerged cultivation of aspergillus foetidus atcc 14916: ph 4.5 at the beginning of cultivation, cultivation for four days, a temperature of 30 degrees c, and an aeration in the fermenter of 1.5 volumes of the air per 1 volume of the medium with a stirring of 280 rpm. the material can be inoculated either as spores (1 x 10(7) spores per 100 ml of the medium) or a material germinated from spores (5% b ... | 1978 | 26852 |
| control of ammonium assimilation in rhizobium 32h1. | the symbiotic, nitrogen-fixing bacterium rhizobium sp. 32h1 is a specialized ammonium producer during symbiosis. however, during free-living growth, rhizobium 32h1 assimilates ammonium very poorly. two pathways of ammonium assimilation exist in enteric bacteria. one is mediated by glutamate dehydrogenase, and the other is mediated by glutamine synthetase-glutamate synthase. the former pathway is altogether inoperative in rhizobium 32h1; the latter pathway operates at a slow rate and is under str ... | 1978 | 27498 |
| differential mutagenicity of reaction products of various pyrazolones with nitrite. | four pyrazolones in frequent use, i.e. antipyrine (ap), aminopyrine (amp), sulpyrine (sp) and isopropylantipyrine (ipa), were compared for their reactivity with nitrite and for the in vitro mutagenicity of their reaction products by ames' reversion tests. in various acidic solutions at 37 degrees c, ap, amp and sp were found to react easily with nitrite and yield various products including dimethylnitrosamine (dmna) and 4-nitrosoantipyrine (4-nap) in the cases of amp and ap, respectively. when t ... | 1978 | 27721 |
| substance p: binding to lipids in the brain. | 1. substance p (sp) could be extracted from brain homogenates with chloroform-methanol by a method which extracts all lipids. 2. sp could be transferred form this total lipid extract (tle) into an aqueous solution at low ph values (2.0--3.0). 3. at higher ph values (5.5) sp could be transferred from an aqueous phase into an organic phase (chloroform:methanol, 2:1) and recombined with tle (which was previously freed from endogenous sp) contained in this phase. the binding capacity of tle for sp e ... | 1978 | 27728 |
| production of valine by a bacillus sp. | a bacterium isolated from burdwan (india) soil was found to accumulate l-valine in the growth medium and identified to be a strain of bacillus subtilis. the strain is able to grow and accumulate valine in a purely synthetic medium, but supplementation of the synthetic medium with either casamino acids or yeast extract or with both, significantly improves the yield. the entire fermentation period can be divided into a growth phase and a production phase, which can be prolonged by adjustment of ph ... | 1978 | 27903 |
| microbial biodegradation of cellophase. | the microbial biodegradation of cellophane (u.c.b.--division sidac) was studied. preliminary experiments with pure cultures of seven cellulolytic microorganisms (aspergillus sp., penicillium sp., chaetomium crispatum, ch. globosum, sclerotium rolfsii and two actinomycetes) revealed that the substrate as such was very recalcitrant, probably due to the occurrence of insoluble coating agents. therefore, mixed cultures of the above mentioned cellulolytic microorganisms were used as inoculum. the cel ... | 1978 | 29381 |
| immunological properties of the lipopolysaccharide-protein complex of coxiella burnetii. | purified lipopolysaccharide-protein complex (lps-pc) extracted by trichloroacetic acid from phase i coxiella burnetii organisms induced in mice and rabbits fair levels of antibodies directed to antigen 1 and antigen 2, as detected by complement-fixation (cf), microagglutination (ma), opsonization-phagocytosis (op) and serum protection (sp) tests. in guinea pigs only very low levels of ma antibodies against antigen 2 were demonstrated. in rabbit serum, ma antibodies directed to antigen 2 were fou ... | 1978 | 29470 |
| purification and properties of amylase produced by a moderately halophilic acinetobacter sp. | a moderately halophilic acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2m nacl or 1m kcl. two kinds of amylase, amylases i and ii were purified from the culture filtrate to an electrophoretically homogenous state by glycogen-complex formation, deae-sephadex a-50 chromatography, and sephadex g-200 gel filtration. both enzymes had maximal activi ... | 1978 | 30530 |
| hydrolysis of phosphatidylethanolamine and phosphatidylglycerol by outer membrane phospholipase a from acinetobacter sp. ho1-n. | an outer membrane phospholipase a active against phosphatidylglycerol and phosphatidylethanolamine was characterized from acinetobacter sp. ho1-n. | 1978 | 30758 |
| production and properties of polygalacturonate lyase by an alkalophilic microorganism bacillus sp. rk9. | bacillus sp. rk9 was isolated from soil and produced a constitutive polygalacturonate lyase. production of the enzyme required the presence of complex nitrogen (peptone and yeast extract). highest activity was obtained with an initial ph of 9.7. the organism was alkalophilic. no growth occurred below ph 7.5. the enzyme was purified by salt precipitation and diethylaminoethyl (deae) cellulose ion-exchange chromatography. the ph optimum for activity was 10.0 in 0.01 m glycine-naoh buffer. calcium ... | 1978 | 31973 |
| adenylate cyclase in bloodstream forms of trypanosoma (trypanozoon) brucei sp. | 1. the adenylate cyclase in trypanosoma brucei is located in the plasma membrane. 2. a partial kinetic analysis of the properties of the enzyme revealed a km for atp of 1.75 mm and a km for mg2+ of 4mm. 3. at low concentrations, mg2+ activated the enzyme directly in addition to its effect of lowering the concentration of inhibitory free atp species. 4. at high concentrations, mg2+ inhibited the enzyme. furthermore, the enzyme was inhibited at any mg2+ concentration if the concentration of atp ex ... | 1978 | 32875 |
| microbial metabolism of amino alcohols. purification and properties of coenzyme b12-dependent ethanolamine ammonia-lyase of escherichia coli. | 1. the 120-fold purification of ethanolamine ammonia-lyase from escherichia coli extracts, to apparent homogeneity, is described. ethanolamine, dithiothreitol, glycerol and kcl protected the apoenzyme from inactivation. 2. at the optimum ph7.5, k(m) values for ethanolamine and coenzyme b(12) were 44mum and 0.42mum respectively. the k(m) for ethanolamine was markedly affected by ph, transitions occurring at ph7.0 and 8.35. 3. the enzyme was specific for ethanolamine as substrate, none of the 18 a ... | 1978 | 33657 |
| ecori activity: enzyme modification or activation of accompanying endonuclease? | a study has been made of the factors and mechanism leading to appearance of the so-called ecori activity described by polisky et al. (1975) in the restrictase ecori preparations. the preparations of purified restrictase ecori, precipitated at 0.9 ammonium sulphate saturation, as well as that obtained using standard techniques have been found to contain an admixture of an endonuclease which at neutral ph and high ionic strength multiply cleaves those dnas which normally have only one recognition ... | 1978 | 33871 |
| production of l-glutamic acid by a bacillus sp. | a strain of bacillus cereus var. mycoides isolated from burdwan soil produces l-glutamate in the medium. the strain is able to grow and produce in a synthetic medium but supplementation with casamino acid or yeast extract improves the yield. maintenance of ph of the fermentation medium near neutrality prolongs the active growth period and improves the yield. glucose and ammonium nitrate were found to be most suitable carbon and nitrogen sources, respectively. cane sugar molasses (as a substitute ... | 1978 | 33882 |
| cell envelope of neisseria gonorrhoeae cs7: peptidoglycan protein complex. | treatment of cells grown to exponential phase with 4% sodium dodecyl sulfate for 3 h at 100 degrees c resulted in solubilization of all cellular components except for peptidoglycan. in most strains, cells cultured in liquid gonococcal broth at ph 7.2 yielded a peptidoglycan composed primarily of n-acetylmuramic acid n-acetylglucosamine, alanine, glutamic acid, and diaminopimelic acid in a molar ratio of 1:1:2:1:1. the peptidoglycan in these cells accounted for 1 to 2% (dry weight) of the cells. ... | 1979 | 33903 |
| substance p: characteristics of binding to synaptic vesicles of rat brain. | 1. the binding of substance p (sp) to synaptic vesicles from rat brain was studied by use of the 125i-tyr8-analogue of sp. 2. the ph dependence of the binding of both peptides to the lipid extractable fraction of synaptic vesicles was shown to be comparable. 3. the binding of 125i-tyr8-sp shows a rate constant of association (k1 = 6.6 x 10(6) m-1 s-1), a rate constant of dissociation (k-1 = 6.4 x 10(-4) s-1) and gives a kd of 1 x 10(-10) m. kd derived from equilibrium studies was 3.2 x 10(-10) m ... | 1979 | 34108 |
| factors affecting the incidence and anti-salmonella activity of the anaerobic caecal flora of the young chick. | thirty-two different types of anaerobic bacteria isolated from chickens have been tested for anti-salmonella activity in vitro. under the conditions of the test only bacteroides hypermegas and a bifidobacterium sp. were shown to inhibit the salmonellas and this was attributed to the production of volatile fatty acids (vfa's) coupled with a low ph. when these organisms were tested in newly hatched chicks no inhibition of s. typhimurium occurred. possible explanations for this observation are cons ... | 1979 | 34658 |
| vasomotor rhinitis--pathophysiological aspects. | vasomotor rhinitis is commonly defined as an unspecific hyperractivity of the nasal mucosa. the symptoms might be due to increased parasympathetic activity to the nose with the release of vaso-secretory active substances. experimental data from the cat suggest that the postganglionic parasympathetic mediator of nasal secretion is cholinergic, whereas the vascular responses appears to be due to a different mechanism. apart from a rich sympathetic and parasympathetic innervation of the nasal mucos ... | 1979 | 34866 |
| [phlegmon of the fore arm succeeding to a dog-bite : respective role of the miscellaneous bacteria involved in this mixed infection (author's transl)]. | the authors report a case of a severe mixed infection (phlegmon of the fore arm) succeeding to a dog bite. a complex flora has been isolated from this phlegmom : pasteurella multocida, pasteurella pneumotropica, streptococcus mitis, actinomyces sp., bacteroides melaninogenicus, and a gram- bacteria related to group ii j. the pathogeny of such diseases is discussed, dwelling in one hand on the part of synergistic development of these various germs and in the other hand on the part played by saliv ... | 1979 | 34886 |
| skin-piercing blood-sucking moths ii: studies on a further 3 adult calyptra [calpe] sp. (lepid., noctuidae). | 1. of the scarce calyptra minuticornis, c. orthograpta and c. labilis, 51, 24, and 7 adults, respectively, were observed during some 600 night inspections at over 100 sites in 1965--1967 and 1971--1977. 2. hitherto biologically completely unknown, and not recorded before in s.e. asia, the latter two species flew in or near tropical monsoon forests in hilly regions (300--600 m) of n. thailand (c. orthograpta also n. laos). c. minuticornis was found in these and in tropical evergreen and semi-ever ... | 1979 | 35931 |
| chikungunya virus in vivo autointerference associated with a small plaque variant. | virulent and avirulent plaque variants of chikungunya (chik) virus were compared with the prototype and other strains. interference with infective virus growth or mouse pathogenicity only occurred in the presence of small plaque (sp) variants in homologous systems. possible mechanisms for this are suggested. | 1978 | 35947 |
| [management in pollinosis and false pollinosis]. | typical cases of pollen allergen (hayfever, allergic asthma), together with isolated non-respiratory "equivalent" manifestations (urticaria, eye conditions, headache, etc.), are easy to detect on the basis of skin tests and the clinical history. such manifestations may also occur in "false pollen allergy", related in most instances by atmospheric moulds (dematiaceae), sometimes by house dust or dermatophytes (candida albicans, trichophyton sp), by food or by a bacterial infection or allergy. a c ... | 1978 | 36022 |
| [effect of a new beta-blocking drug (sp/1601) on the extension of experimental acute infarct in anesthetized dogs]. | | 1977 | 36114 |
| emulsifier of arthrobacter rag-1: isolation and emulsifying properties. | the oil-degrading arthrobacter sp. rag-1 produced an extracellular nondialyzable emulsifying agent when grown on hexadecane, ethanol, or acetate medium. the emulsifier was prepared by two procedures: (i) heptane extraction of the cell-free culture medium and (ii) precipitation with ammonium sulfate. a convenient assay was developed for measurement of emulsifier concentrations between 3 and 75 micrograms/ml. the rate of emulsion fromation was proportional to both hydrocarbon and emulsifier concen ... | 1979 | 36840 |
| mouse kidney trehalase: purification and properties. | trehalase (e.c.3.2.1.28) was isolated from mouse kidney and purified to homogeneity. the enzyme was solubilized with n-octanol and activated by freezing and thawing before precipitation with ammonium sulfate. a 1700-fold purification was achieved by chromatography on deae-cellulose, sp-sephadex, followed by gel filtration on sephadex g-200. only a single form of enzyme activity was shown throughout the fractionation as confirmed by gel electrophoresis of the final preparation. the enzyme was spe ... | 1979 | 37791 |