| molecular characterization of a distinct potyvirus from whitegrass in china. | apotyvirus isolated from perennial whitegrass ( pennisetum centrasiaticum tzvel.) in north china was characterized at the molecular level. the 3' terminal nucleotide (nt) sequence of 1669 nt of the viral rna genome has been determined, which covered the coding region of the c-terminal part of the large nuclear inclusion protein (nib, rna polymerase), capsid protein (cp) gene and the 3' nontranslated region (ntr). the cp gene consisted of 909 nt (including the stop codon) encoding 302 amino acid ... | 2003 | 12756626 |
| characterization of a distinct johnsongrass mosaic virus strain isolated from sorghum in nigeria. | a virus isolated from sorghum in nigeria has been partially characterized. it was tested by enzyme-linked immunosorbent assay (elisa) using antisera to maize dwarf mosaic virus, johnsongrass mosaic virus (jgmv), sugarcane mosaic virus strain-mdb, sorghum mosaic virus, and zea mosaic virus. a partial host range, symptom phenotypes for selected sorghum lines, and the mass of the coat protein (cp) subunit was analyzed by sodium-dodecyl sulfate polyacrylamide gel electrophoresis (sds-page), and its ... | 2005 | 15503220 |
| analysis of sugarcane mosaic virus resistance in maize in an isogenic dihybrid crossing scheme and implications for breeding potyvirus-resistant maize hybrids. | the gene action of 2 sugarcane mosaic virus (scmv) resistance loci in maize, scmv1 and scmv2, was evaluated for potyvirus resistance in an isogenic background. all 4 homozygous and 5 heterozygous isogenic genotypes were produced for introgressions of the resistant donor (fap1360a) alleles at both loci into the susceptible parent (f7) genetic background using simple sequence repeat markers. for scmv and maize dwarf mosaic virus (mdmv), virus symptoms appeared rapidly in the 3 homozygous genotypes ... | 2006 | 17213909 |
| characterization of a novel potyvirus isolated from maize in israel. | a potyvirus (proposed name of zea mosaic virus [zemv]) isolated from maize in israel was analyzed by serology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of capsid proteins, symptomatology, and sequencing. parts of the nuclear inclusion b, coat protein, and 3' regions were sequenced; the amino acid sequence of zemv capsid was determined by time-of-flight mass spectrometry (tofms). the results of these analyses were compared with those of similar analyses of the followin ... | 2000 | 18944557 |
| iranian johnsongrass mosaic virus: the complete genome sequence, molecular and biological characterization, and comparison of coat protein gene sequences. | iranian johnsongrass mosaic virus (ijmv) is one of the most prevalent viruses causing maize mosaic disease in iran. an ijmv isolate, maz-bah, was obtained from the maize showing mosaic symptoms in mazandaran, north of iran. the complete genomic sequence of maz-bah is 9544 nucleotides, excluding the poly(a) tail. it contains one single open reading frame of 9165 nucleotides and encodes a large polyprotein of 3054 amino acids, flanked by a 5'-untranslated region (utr) of 143 nucleotides and a 3'-u ... | 2017 | 27632283 |