| measurement of the cytotoxic effects of different strains of mycoplasma equigenitalium on the equine uterine tube using a calmodulin assay. | the cytopathic effects induced by five strains of mycoplasma equigenitalium for cells of equine uterine tube explants were tested by measuring changes in cellular and extracellular concentrations of calmodulin (cam). calmodulin concentrations in samples of total homogenate (th) and total homogenate supernates (ths) of the infected equine uterine tube explants were significantly lower than respective measurements on noninfected controls. in tissue culture medium fractions (tcm) of some infected e ... | 1992 | 1477802 |
| effect of sample freezing on the isolation of mycoplasma spp. from the clitoral fossa of the mare. | the growth of mycoplasma equigenitalium and mycoplasma subdolum from specimens collected from the clitoral fossa of each of four standardbred mares was not diminished by freezing of the specimens in liquid nitrogen (-196 degrees c) for up to 30 days when compared to samples cultured immediately. | 1988 | 3349394 |
| characteristics of mycoplasma strains isolated from stallion semen. | eleven mycoplasma strains were isolated from the semen of 24 stallions. eight of these strains were identified as mycoplasma equigenitalium. three strains which hydrolized arginine could not be identified. the growth inhibition test with immune sera against m. arginini and m. equirhinis was negative. antibiotic sensitivity test showed that all strains were sensitive to four antibiotic of tetracycline group (oxytetracyclin, minocycline, transcycline and vibramycin). lincomycin and gentamycin appe ... | 1985 | 3833120 |
| [mycoplasmas isolated from the genital tract of mares (author's transl)]. | mycoplasmas were isolated from 11 (=#6,8%) of 161 cervix swabs from infertile mares. a total of 17 strains was isolated and characterized by indirect immunofluorescent test and metabolic inhibition test as mycoplasma equigenitalium (11 strains), mycoplasma subdolum (2 strains), acholeplasma laidlawii (3 strains) and acholeplasma equifetale (1 strain). in addition cervix swabs of the mares were investigated for bacteria. there was no specific correlation between presence of mycoplasmas and bacter ... | 1980 | 6999784 |
| re-evaluation of the classical mycoplasma lipophilum cluster (weisburg et al. 1989) and description of two new clusters in the hominis group based on 16s rdna sequences. | the mycoplasma lipophilum cluster (weisburg et al. 1989) in the hominis group of the mollicutes is re-evaluated in this work to update the phylogenetic framework for classification of species within the genus mycoplasma. therefore, sequences of the 16s rrna gene were determined from previously described species, and 11 were found to be closely related to the m. lipophilum cluster. a selection of members of the other hitherto defined clusters of the hominis group was included for phylogenetic ana ... | 2001 | 11321109 |
| high prevalence of mycoplasmas in the genital tract of asymptomatic stallions in austria. | mycoplasma equigenitalium and m. subdolum have been implicated in genital disorders and infertility of horses. the reported cytopathic effects of m. equigenitalium observed in vitro underscore its potential pathogenic role in reproductive dysfunction in mares. this study was initiated to determine the prevalence of mycoplasmas in the genital tract of stallions in relationship to age, clinical signs, geographic location and semen quality. for this purpose the mycoplasma flora of the genital tract ... | 2002 | 12034540 |
| phase and size variable surface-exposed proteins in equine genital mycoplasmas. | mycoplasma equigenitalium and mycoplasma subdolum have been associated with infertility, endometritis, vulvitis and abortions in mares, and with reduced fertility and balanoposthitis in stallions. despite their role in equine genital disorder, determinants of virulence and pathogenesis as well as factors provoking specific host immune responses have not been identified, so far. to establish the major immunogenic components of mycoplasma (m.) equigenitalium and m. subdolum, antigen profiles of th ... | 2005 | 16143468 |
| recovery of mycoplasma spp. from the reproductive tract of the mare during the estrous cycle. | the sites in the genital tract from which mycoplasmas could be recovered at various stages of the estrous cycle were studied in five standardbred mares naturally infected with mycoplasma. mycoplasma equigenitalium and mycoplasma subdolum were most frequently isolated from the clitoral fossa as compared to the vagina, cervix, and uterus. the lowest isolation prevalence was observed in the uterus. the recovery of mycoplasma spp. from the clitoral fossa did not differ at any stage of the estrous cy ... | 1987 | 17422844 |
| isolation and molecular identification of mycoplasma equigenitalium from equine genital tracts in northern india. | although mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. to address this limitation, the authors developed and optimized species-specific primer pairs that target m. eguigenitalium rpob (rna polymerase b subunit) gene sequences. the specificity of the pcr assay developed in this study was determined using 12 field isolates including the type strain of m. equigenitalium and other myco ... | 2015 | 27175172 |
| pilot study to evaluate the role of mycoplasma species in cat bite abscesses. | mycoplasma species are common inhabitants of the feline oral cavity, and so likely contaminate many cat bite abscesses. the objectives of this study were to determine whether mycoplasma species are common contaminants of cat bite abscesses and whether they are are associated with β-lactam-resistant clinical disease. twenty-six privately owned cats with clinical evidence of an abscess suspected to be from a cat bite were included in the study. samples from each cat were evaluated by aerobic and a ... | 2014 | 24643287 |