| the archaeal halophilic virus-encoded dam-like methyltransferase m. phich1-i methylates adenine residues and complements dam mutants in the low salt environment of escherichia coli. | the genome of the archaeal virus phich1, infecting natrialba magadii (formerly natronobacterium magadii), is composed of 58.5 kbp linear ds dna. virus particles contain several rna species in sizes of 100-800 nucleotides. a fraction of phich1 genomes is modified within 5'-gatc-3' and related sequences, as determined by various restriction enzyme digestion analyses. high performance liquid chromatography revealed a fifth base, in addition to the four nucleosides, which was identified as n6-methyl ... | 2000 | 10712697 |
| the structural protein e of the archaeal virus phich1: evidence for processing in natrialba magadii during virus maturation. | phich1 is a lysogenic virus for the haloalkalophilic archaeon natrialba magadii. the virus morphology resembles other members of myoviridae infecting halobacterium species. the gene of the major capsid protein e of virus phich1 was cloned and the dna sequence was determined. gene e was mapped to a 3.2-kbp clai fragment, localized to the 5'-end of the phich1 genome. the complete nucleotide sequence of this region was determined and the identity of gene e was confirmed by comparing the experimenta ... | 2000 | 11040128 |
| natrialba magadii virus phich1: first complete nucleotide sequence and functional organization of a virus infecting a haloalkaliphilic archaeon. | the double-stranded (ds)dna virus phich1 infects the haloalkaliphilic archaeon natrialba magadii. the complete dna sequence of 58 498 bp of the temperate virus was established, and the probable functions of 21 of 98 phich1-encoded open reading frames (orfs) have been assigned. this knowledge has been used to propose functional modules each required for specific functions during virus development. the phich1 dna is terminally redundant and circularly permuted and therefore appears to be packaged ... | 2002 | 12139629 |
| the lysogenic region of virus phich1: identification of a repressor-operator system and determination of its activity in halophilic archaea. | phich1 is a temperate virus infecting the haloalkaliphilic archaeon natrialba magadii. as for all temperate viruses, a control of the lysogenic state versus the lytic life cycle is essential. two open reading frames (orfs) have been identified as putative repressor encoding genes: orf48 and orf49. the protein of orf48 showed sequence similarities to putative repressor molecules. orf49 was identified by the analysis of a mutant of phich1: the lysogenic strain carrying mutant phich1-1 showed a dif ... | 2007 | 17123129 |