| antitumor polyketide biosynthesis by an uncultivated bacterial symbiont of the marine sponge theonella swinhoei. | bacterial symbionts have long been suspected to be the true producers of many drug candidates isolated from marine invertebrates. sponges, the most important marine source of biologically active natural products, have been frequently hypothesized to contain compounds of bacterial origin. this symbiont hypothesis, however, remained unproven because of a general inability to cultivate the suspected producers. however, we have recently identified an uncultured pseudomonas sp. symbiont as the most l ... | 2004 | 15520376 |
| [microbial community structure in bio-ceramics and biological activated carbon analyzed by pcr-sscp technique]. | analyses of microbial community structure in bio-ceramics (bc) and biological activated carbon (bac), which widely used in drinking water treatment were performed by polymerase-chain-reaction-single-strand-conformation-polymorphism (pcr-sscp) targeted eubacterial 16s ribosomal rna gene. microorganisms on bio-ceramics and biological activated carbon were detached by ultrasonic, culturing on r2a and lb agar, respectively, followed by genome dna extracting. results show that larger than 10 kb genom ... | 2007 | 17639962 |
| pseudomonas community structure and antagonistic potential in the rhizosphere: insights gained by combining phylogenetic and functional gene-based analyses. | the pseudomonas community structure and antagonistic potential in the rhizospheres of strawberry and oilseed rape (host plants of the fungal phytopathogen verticillium dahliae) were assessed. the use of a new pcr-dgge system, designed to target pseudomonas-specific gaca gene fragments in environmental dna, circumvented common biases of 16s rrna gene-based dgge analyses and proved to be a reliable tool to unravel the diversity of uncultured pseudomonas in bulk and rhizosphere soils. pseudomonas-s ... | 2007 | 17686023 |
| identification and molecular modeling of a novel lipase from an antarctic soil metagenomic library. | in this work, we present the construction of a metagenomic library in escherichia coli using puc19 vector and environmental dna directly isolated from antarctic topsoil and screened for lipolytic enzymes. screening on agar supplemented with olive oil and rhodamine b revealed one clone with lipolytic activity (lip1) out of 1000 e. coli clones. this clone harbored a plasmid, plip1, which has an insert of 4722 bp that was completely sequenced from both directions. further analysis of the insert sho ... | 2009 | 19899611 |