| alkaliphilus transvaalensis gen. nov., sp. nov., an extremely alkaliphilic bacterium isolated from a deep south african gold mine. | a novel extreme alkaliphile was isolated from a mine water containment dam at 3.2 km below land surface in an ultra-deep gold mine near carletonville, south africa. the cells of this bacterium were straight to slightly curved rods, motile by flagella and formed endospores. growth was observed over the temperature range 20-50 degrees c (optimum 40 degrees c; 45 min doubling time) and ph range 8.5-12.5 (optimum ph 10.0). the novel isolate, one of the most alkaliphilic micro-organisms yet described ... | 2001 | 11491320 |
| salinicoccus alkaliphilus sp. nov., a novel alkaliphile and moderate halophile from baer soda lake in inner mongolia autonomous region, china. | a novel alkaliphilic and moderately halophilic gram-positive coccus, designated strain t8t, was isolated from baer soda lake in inner mongolia autonomous region, china. strain t8t grew in the presence of 0-25% (w/v) nacl and at ph 6.5-11.5, with optimum growth at 10% (w/v) nacl and ph 9.0. it grew at 10.0-46.0 degrees c, with an optimum growth temperature of 32.0 degrees c. the organism was strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. the dna g+c content was ... | 2002 | 12054239 |
| alkaliphilus crotonatoxidans sp. nov., a strictly anaerobic, crotonate-dismutating bacterium isolated from a methanogenic environment. | two bacterial strains were isolated from methanogenic butyrate-oxidizing mixed cultures. the cells were straight to slightly curved, gram-positive rods that were motile by means of multiple flagella and formed endospores. growth was observed in the temperature range 15-45 degrees c (optimum 37 degrees c) and ph range 5.5-9.0 (optimum ph 7.5). the novel isolates were strictly anaerobic chemo-organotrophs capable of utilizing yeast extract, peptone, tryptone and a variety of sugars and organic aci ... | 2003 | 12892113 |
| marinobacter alkaliphilus sp. nov., a novel alkaliphilic bacterium isolated from subseafloor alkaline serpentine mud from ocean drilling program site 1200 at south chamorro seamount, mariana forearc. | novel alkaliphilic, mesophilic bacteria were isolated from subseafloor alkaline serpentine mud from the ocean drilling program (odp) hole 1200d at a serpentine mud volcano, south chamorro seamount in the mariana forearc. the cells of type strain odp1200d-1.5t were motile rods with a single polar flagellum. growth was observed between 10 and 45-50 degrees c (optimum temperature: 30-35 degrees c, 45-min doubling time), between ph 6.5 and 10.8-11.4 (optimum: ph 8.5-9.0), and between nacl concentrat ... | 2005 | 15322951 |
| alkaline anaerobic respiration: isolation and characterization of a novel alkaliphilic and metal-reducing bacterium. | iron-reducing enrichments were obtained from leachate ponds at the u.s. borax company in boron, calif. based on partial small-subunit (ssu) rrna gene sequences (approximately 500 nucleotides), six isolates shared 98.9% nucleotide identity. as a representative, the isolate qymf was selected for further analysis. qymf could be grown with fe(iii)-citrate, fe(iii)-edta, co(iii)-edta, or cr(vi) as electron acceptors, and yeast extract and lactate could serve as electron donors. growth during iron red ... | 2004 | 15345448 |
| extremely high alkaline protease from a deep-subsurface bacterium, alkaliphilus transvaalensis. | a new high-alkaline protease (altp) was purified to homogeneity from a culture of the strictly anaerobic and extremely alkaliphilic alkaliphilus transvaalensis. the molecular mass was 30 kda on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme showed the maximal caseinolytic activity higher than ph 12.6 in kcl-naoh buffer at 40 degrees c. hydrolysis of the oxidized insulin b-chain followed by mass spectrometric analysis of the cleaved products revealed that as many as 24 of t ... | 2007 | 17216443 |
| salinicoccus jeotgali sp. nov., isolated from jeotgal, a traditional korean fermented seafood. | a novel, moderately halophilic, gram-positive coccus, designated strain s2r53-5(t), was isolated from jeotgal, a traditional korean fermented seafood. the organism was strictly aerobic, non-motile, non-sporulating and catalase- and oxidase-positive. strain s2r53-5(t) grew in the presence of 0.5-15 % (w/v) nacl and at ph 6.5-11.0, with optimum growth at 5 % (w/v) nacl and ph 7.0. the temperature range for growth was 20.0-30.0 degrees c, with an optimum temperature of 30 degrees c. phylogenetic an ... | 2007 | 17329798 |
| maritimibacter alkaliphilus gen. nov., sp. nov., a genome-sequenced marine bacterium of the roseobacter clade in the order rhodobacterales. | a gram-negative, chemoheterotrophic, strictly aerobic, alkaliphilic, rod-shaped marine bacterium, designated htcc2654(t), was isolated from the western sargasso sea by using a dilution-to-extinction culturing method. phylogenetic analyses based on 16s rrna gene sequences showed that strain htcc2654(t) belonged to the roseobacter clade of the order rhodobacterales. the 16s rrna gene sequence similarity of the strain with respect to other members of the roseobacter clade ranged from 90.4 to 95.1 % ... | 2007 | 17625211 |
| salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in yunnan, south-west china. | a novel yellow-pigmented, non-motile, non-sporulating, catalase- and oxidase-positive, obligately aerobic, moderately halophilic, facultatively alkaliphilic gram-positive coccus, strain yim y15(t), was isolated from a brine sample from a salt mine in yunnan, south-west china. strain yim y15(t) grew in the presence of 0.5-25 % (w/v) nacl and at ph 6.0-10.0, with optimum growth at 8-10 % (w/v) nacl and ph 8.0. it grew at 4-45 degrees c, with optimum growth at 37.0 degrees c. the major cellular fat ... | 2007 | 17911305 |
| dethiobacter alkaliphilus gen. nov. sp. nov., and desulfurivibrio alkaliphilus gen. nov. sp. nov.: two novel representatives of reductive sulfur cycle from soda lakes. | anaerobic enrichments with h2 as electron donor and thiosulfate/polysulfide as electron acceptor at ph 10 and 0.6 m total na+ yielded two non sulfate-reducing representatives of reductive sulfur cycle from soda lake sediments. strain aht 1 was isolated with thiosulfate as the electron acceptor from north-eastern mongolian soda lakes and strain aht 2-with polysulfide as the electron acceptor from wadi al natrun lakes in egypt. both isolates represented new phylogenetic lineages: aht 1-within clos ... | 2008 | 18317684 |
| transformation of inorganic and organic arsenic by alkaliphilus oremlandii sp. nov. strain ohilas. | alkaliphilus oremlandii sp. nov. strain ohilas is a mesophilic, spore-forming, motile, low mole%gc gram positive. it was enriched from ohio river sediments on a basal medium with 20 mm lactate and 5 mm arsenate and isolated through passage on medium with increased arsenic concentration (10 and 20 mm), tindalization, and serial dilution. the ph optimal for growth was 8.4 and 16s rrna gene sequence analysis indicated it is most closely related to species in the genus alkaliphilus (a. crotonoxidans ... | 2008 | 18378595 |
| halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in fuente de piedra, southern spain. | a gram-positive, spore-forming, halophilic bacterial strain, fp5t, was isolated from a salt lake in southern spain and subjected to a polyphasic taxonomic study. strain fp5t was strictly aerobic. cells were coccoidal, occurring singly or in clusters. the cell-wall peptidoglycan type of strain fp5t was a4 beta based on l-orn-d-asp. strain fp5t was characterized chemotaxonomically by having mk-7 as the major menaquinone and anteiso-c15 : 0, anteiso-c17 : 0, iso-c15 : 0 and iso-c16 : 0 as the main ... | 2008 | 18398188 |
| the selenoproteome of clostridium sp. ohilas: characterization of anaerobic bacterial selenoprotein methionine sulfoxide reductase a. | selenocysteine (sec) is incorporated into proteins in response to uga codons. this residue is frequently found at the catalytic sites of oxidoreductases. in this study, we characterized the selenoproteome of an anaerobic bacterium, clostridium sp. (also known as alkaliphilus oremlandii) ohila, and identified 13 selenoprotein genes, five of which have not been previously described. one of the detected selenoproteins was methionine sulfoxide reductase a (msra), an antioxidant enzyme that repairs o ... | 2009 | 18767149 |
| halolactibacillus alkaliphilus sp. nov., a moderately alkaliphilic and halophilic bacterium isolated from a soda lake in inner mongolia, china, and emended description of the genus halolactibacillus. | a moderately alkaliphilic and halophilic bacterium was isolated from sediment of xiarinaoer soda lake located in the inner mongolia municipality. this bacterium, designated strain h-5(t), was a facultative anaerobe, gram-positive, rod-shaped and non-motile. strain h-5(t) grew in complex medium with 0.5-30% (w/v) nacl and at ph 7.5-13. the cell wall peptidoglycan contained meso-diaminopimelic acid. the major isoprenoid quinones found in this strain were mk-9h(4) and mk-9h(2), and the major cellul ... | 2008 | 18768624 |
| lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the yellow sea. | a gram-negative, non-motile, rod-shaped bacterial strain, designated smk-117t, belonging to the alphaproteobacteria, was isolated from a tidal flat of the yellow sea, korea, and was subjected to a polyphasic taxonomic study. strain smk-117t grew optimally at ph 7.0-8.0 and 30 degrees c in the presence of 2 % (w/v) nacl. neighbour-joining and maximum-likelihood phylogenetic trees based on 16s rrna gene sequences showed that strain smk-117t clustered with maritimibacter alkaliphilus htcc2654t, wit ... | 2009 | 19126722 |
| nitriliruptor alkaliphilus gen. nov., sp. nov., a deep-lineage haloalkaliphilic actinobacterium from soda lakes capable of growth on aliphatic nitriles, and proposal of nitriliruptoraceae fam. nov. and nitriliruptorales ord. nov. | a novel bacterial strain, designated anl-iso2(t), was obtained from an enrichment culture inoculated with a mixture of soda lake sediments by using isobutyronitrile (ibn) as the carbon, energy and nitrogen source at ph 10. the enrichment resulted in a stable binary culture containing ibn-degrading gram-positive rods and a satellite gram-negative gammaproteobacterium marinospirillum sp. strain (anl-isoa) scavenging the products of nitrile hydrolysis. cells of the ibn-degrading strain, anl-iso2(t) ... | 2009 | 19196761 |
| respiratory arsenate reductase as a bidirectional enzyme. | the haloalkaliphilic bacterium alkalilimnicola ehrlichii is capable of anaerobic chemolithoautotrophic growth by coupling the oxidation of arsenite (as(iii)) to the reduction of nitrate and carbon dioxide. analysis of its complete genome indicates that it lacks a conventional arsenite oxidase (aox), but instead possesses two operons that each encode a putative respiratory arsenate reductase (arr). here we show that one homolog is expressed under chemolithoautotrophic conditions and exhibits both ... | 2009 | 19285953 |
| four novel arthrobacter species isolated from filtration substrate. | four gram-positive, non-motile, non-spore-forming bacterial strains, lc4(t), lc6(t), lc10(t) and lc13(t), were isolated from a filtration substrate made from trass, a volcanic rock, and their taxonomic positions were investigated by a polyphasic taxonomic approach. the novel strains grew over a temperature range of 5-40 degrees c, at ph values of 6-11 and in the presence of 3-7 % (w/v) nacl. a phylogenetic tree based on 16s rrna gene sequences showed the novel strains formed a distinct evolution ... | 2009 | 19329620 |
| halobacillus naozhouensis sp. nov., a moderately halophilic bacterium isolated from a sea anemone. | a moderately halophilic, gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain jsm 071068(t), was isolated from a sea anemone (anthopleura xanthogrammica) collected from the naozhou island on the leizhou bay in the south china sea. cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. strain jsm 071068(t) was able to grow with 1-20% (w/v) total salts (optimum, 6-9%), at ph values of ... | 2009 | 19370398 |
| properties of arsenite efflux permeases (acr3) from alkaliphilus metalliredigens and corynebacterium glutamicum. | members of the acr3 family of arsenite permeases confer resistance to trivalent arsenic by extrusion from cells, with members in every phylogenetic domain. in this study bacterial acr3 homologues from alkaliphilus metalliredigens and corynebacterium glutamicum were cloned and expressed in escherichia coli. modification of a single cysteine residue that is conserved in all analyzed acr3 homologues resulted in loss of transport activity, indicating that it plays a role in acr3 function. the result ... | 2009 | 19494117 |
| halobacillus hunanensis sp. nov., a moderately halophilic bacterium isolated from a subterranean brine. | a moderately halophilic, gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain jsm 071077(t), was isolated from a subterranean brine sample collected from a salt mine in hunan province, china. cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. strain jsm 071077(t) was able to grow with 2-25% (w/v) total salts (optimum, 5-10%), at ph 6.0-10.0 (optimum, ph 7.5) and 10-40 degrees c ( ... | 2009 | 19618289 |
| halobacillus salsuginis sp. nov., a moderately halophilic bacterium from a subterranean brine. | a moderately halophilic, endospore-forming, gram-positive, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium, designated strain jsm 078133(t), was isolated from a subterranean brine sample collected from a salt mine in hunan province, china. strain jsm 078133(t) was able to grow with 1-23 % (w/v) total salts (optimum, 5-10 %) and at ph 6.0-10.0 (optimum, ph 7.5) and 10-45 degrees c (optimum, 30 degrees c). meso-diaminopimelic acid was present in the cell-wall peptidoglycan. t ... | 2009 | 19622644 |
| algoriphagus olei sp. nov., isolated from oil-contaminated soil. | a motile, gram-negative, pinkish red-pigmented bacterium (strain cc-hsuan-617(t)) was isolated from an oil-contaminated soil near an oil refinery located in kaohsiung county, taiwan. 16s rrna gene sequence analysis showed that strain cc-hsuan-617(t) clustered with algoriphagus mannitolivorans (97.5 % sequence similarity), algoriphagus aquatilis (98.0 %) and algoriphagus boritolerans (97.5 %), followed by algoriphagus ornithinivorans (97.1 %) and algoriphagus alkaliphilus (97.1 %). the fatty acid ... | 2009 | 19628616 |
| indibacter alkaliphilus gen. nov., sp. nov., a novel alkaliphilic bacterium isolated from haloalkaline lonar lake, india. | a novel gram-negative, rod shaped, non-motile bacterium, strain lw1t, was isolated from a water sample collected at a depth of 3.5 m from the lonar lake of buldhana district, maharastra, india. the cell suspension was reddish-orange due to the presence of carotenoids. cells of the strain lw1t are positive for catalase, oxidase, ornithine decarboxylase and lysine decarboxylase and negative for gelatinase, urease and lipase. the fatty acids were dominated by branched-chain fatty acids (>76 %), wit ... | 2009 | 19656931 |
| [new alkaliphilic peptide fermenting and fe(iii) regenerating bacteria alkaliphilus peptidofermentans sp.nov. from soda lake]. | | 2009 | 19827715 |
| propionate and butyrate dependent bacterial sulfate reduction at extremely haloalkaline conditions and description of desulfobotulus alkaliphilus sp. nov. | evidence on the utilization of simple fatty acids by sulfate-reducing bacteria (srb) at extremely haloalkaline conditions are practically absent, except for a single case of syntrophy by desulfonatronum on acetate. our experiments with sediments from soda lakes of kulunda steppe (altai, russia) showed sulfide production with sulfate as electron acceptor and propionate and butyrate (but not acetate) as an electron donor at a ph 10-10.5 and a salinity 70-180 g l(-1). with propionate as substrate, ... | 2010 | 19888546 |
| euzebya tangerina gen. nov., sp. nov., a deeply branching marine actinobacterium isolated from the sea cucumber holothuria edulis, and proposal of euzebyaceae fam. nov., euzebyales ord. nov. and nitriliruptoridae subclassis nov. | a tangerine-coloured, gram-positive actinobacterial strain, designated f10(t), was isolated from the abdominal epidermis of a sea cucumber, holothuria edulis, collected in seawater off the coast of japan. a 16s rrna gene sequence analysis indicated that strain f10(t) was a member of the class actinobacteria and was most closely related to nitriliruptor alkaliphilus anl-iso2(t) (87.4 % sequence similarity). phylogenetic analyses showed that strain f10(t) represented a novel, deep-rooted, and dist ... | 2010 | 19915105 |
| alkaliphilus halophilus sp. nov., a strictly anaerobic and halophilic bacterium isolated from a saline lake, and emended description of the genus alkaliphilus. | a novel strictly anaerobic, halophilic and fermentative strain, designated e2r(t), was isolated from sediments of xiaokule salt lake in xinjiang province, china. cells were straight to slightly curved, gram-stain-positive rods that were motile by means of flagella and formed endospores. strain e2r(t) was moderately halophilic and grew optimally in the presence of 7.5 % nacl, at ph 8.0 and at 32°c. substrates used include yeast extract, casamino acids, tryptone, fructose, sucrose, xylose, ribose, ... | 2010 | 20097801 |
| effect of salinity on denitrification under limited single carbon source by marinobacter sp. isolated from marine sediment. | marinobacter comprises gram-negative, aerobic, motile, and rod-shaped bacteria within the gamma-subclass of the proteobacteria and is known to be halophilic or halotolerant, heterotrophic neutrophile. two strains classified as belonging to marinobacter, named pad-2 and set-1, were isolated from marine sediment. the most closely related species of pad-2 and set-1 are m. alkaliphilus and m. guinea, respectively. the strain pad-2 exhibited remarkably higher denitrification at concentrations of 0.5 ... | 2010 | 20143355 |
| indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. | a novel gram-stain-negative, rod-shaped, non-motile bacterium, strain lw1(t), was isolated from a water sample collected at a depth of 3.5 m from lonar lake, buldhana district, maharashtra, india. the cell suspension was reddish-orange due to the presence of carotenoids. strain lw1(t) was positive for catalase, oxidase, ornithine decarboxylase and lysine decarboxylase and negative for gelatinase, urease and lipase. fatty acids were dominated by branched-chain fatty acids (>76 %), with a high abu ... | 2010 | 20371870 |
| salinicoccus kekensis sp. nov., a novel alkaliphile and moderate halophile isolated from keke salt lake in qinghai, china. | a novel alkaliphilic and moderate halophilic bacterium, designated strain k164(t), was isolated from keke salt lake in qinghai, china. the strain grew with 2.0-20.0% (w/v) nacl, at 4-50 degrees c and ph 6.5-11.5, with an optimum of 8% (w/v) nacl, 37degrees c and ph 10, respectively. the predominant respiratory quinone was menaquinone 6 (mk-6) and the major polar lipid was phosphatidylethanolamine. the major cellular fatty acids were anteiso-c(15:0) and iso-c(15:0). the genomic dna g+c content wa ... | 2010 | 20449768 |
| biocatalytic resolution of glycidyl phenyl ether using a novel epoxide hydrolase from a marine bacterium, maritimibacter alkaliphilus kccm 42376 [corrected]. | as a continuous effort of developing highly enantioselective epoxide hydrolase from marine microorganisms, it was found that maritimibacter alkaliphilus kccm 42376 [corrected] was highly enantioselective toward racemic glycidyl phenyl ether (gpe). an open reading frame (orf) encoding a putative epoxide hydrolase (ehase) was cloned from the genome of maritimibacter alkaliphilus kccm 42376 [corrected], followed by expression and purification in escherichia coli. the purified ehase (reh) hydrolyzed ... | 2010 | 20471590 |
| biochemical characterization of a novel arsa atpase complex from alkaliphilus metalliredigens qymf. | the two putative ars operons in alkaliphilus metalliredigens qymf are distinctive in that the arsa gene is split in halves, amarsa1 and amarsa2, and, acr3 but not an arsb gene coexists with arsa. heterologous expression of one of the a. metalliredigensars operons (ars1) conferred arsenite but not antimonite resistance to deltaarsescherichia coli. only the co-expressed amarsa1 and amarsa2 displayed arsenite or antimonite stimulated atpase activity. the results show that amarsa1-amarsa2 interactio ... | 2010 | 20553716 |
| genome sequences of pelagibaca bermudensis htcc2601t and maritimibacter alkaliphilus htcc2654t, the type strains of two marine roseobacter genera. | pelagibaca bermudensis htcc2601(t) and maritimibacter alkaliphilus htcc2654(t) represent two marine genera in the globally significant roseobacter clade of the alphaproteobacteria. here, we present the genome sequences of these organisms, isolated from the sargasso sea using dilution-to-extinction culturing, which offer insight into the genetic basis for the metabolic and ecological diversity of this important group. | 2010 | 20729358 |
| cecembia lonarensis gen. nov., sp. nov., a novel haloalkalitolerant bacterium of the family cyclobacteriaceae, isolated from a haloalkaline lake and emended descriptions of the genera indibacter, nitritalea, belliella and aquiflexum. | a novel gram-staining-negative, rod shaped, non-motile bacterium, designated strain lw9t, was isolated from a water sample collected at a depth of 4.5 m from lonar lake of buldhana district, maharastra, india. the cell suspension (broth culture) was reddish-orange due to the presence of carotenoid pigments. strain lw9t was positive for catalase, ornithine decarboxylase and lysine decarboxylase activities and negative for gelatinase, oxidase, urease and lipase activities. the predominant fatty ac ... | 2010 | 20851918 |
| a proteome investigation of roxarsone degradation by alkaliphilus oremlandii strain ohilas. | clostridial species predominate in both chicken gastrointestinal tract as well as litter where the organoarsenical roxarsone (3-nitro 4-hydroxybenzenearsonic acid) is anaerobically transformed releasing the more recognized toxic inorganic arsenic. 2d-gel electrophoresis and mass spectrometry were used to evaluate the changes in protein expression of alkaliphilus oremlandii in response to different growth conditions (e.g., terminal electron acceptors) in order to explore the mechanism of microbia ... | 2010 | 21069144 |
| tandem use of selenocysteine: adaptation of a selenoprotein glutaredoxin for reduction of selenoprotein methionine sulfoxide reductase. | several engineered selenocysteine (sec)-containing glutaredoxins (grxs) and their enzymatic properties have been reported, but natural selenoprotein grxs have not been previously characterized. we expressed a bacterial selenoprotein grx from clostridium sp. (also known as alkaliphilus oremlandii) ohilas in escherichia coli and characterized this selenoenzyme and its natural cys homologues in clostridium and e. coli. the selenoprotein grx had a 200-fold higher activity than its sec-to-cys mutant ... | 2011 | 21210868 |
| probing the enantioselectivity of a diverse group of purified cobalt-centred nitrile hydratases. | in this study a diverse range of purified cobalt containing nitrile hydratases (nhases, ec 4.2.1.84) from rhodopseudomonas palustris haa2 (haa2), rhodopseudomonas palustris cga009 (009), sinorhizobium meliloti 1021 (1021), and nitriliruptor alkaliphilus (iso2), were screened for the first time for their enantioselectivity towards a broad range of chiral nitriles. enantiomeric ratios of >100 were found for the nhases from haa2 and cga009 on 2-phenylpropionitrile. in contrast, the fe-containing nh ... | 2011 | 21380438 |
| algoriphagus jejuensis sp. nov., isolated from seawater. | a gram-negative, pink-pigmented, non-motile, strictly aerobic, rod-shaped bacterium, designated cnu040t, was isolated from seawater on the coast of jeju island in korea, and subjected to a polyphasic taxonomic study. the temperature, ph and nacl ranges for growth were 4-30 °c, ph 5.5-10.0 and 0-5.0 %, respectively. phylogenetic analysis based on 16s rrna gene sequences indicated that strain cnu040t belongs to a distinct lineage in the genus algoriphagus, with high sequence similarity to algoriph ... | 2011 | 21460141 |
| salinicoccus qingdaonensis sp. nov., a novel bacterium isolated from coastal seawater of qingdao during a bloom of green algae. | a novel gram-positive, white-pigmented, non-motile, non-sporulating, catalase- and oxidase- positive, strictly aerobic coccus, designated strain zxm223t, was isolated from a seawater sample collected from the coast of qingdao. it grew at ph 6.0-10.5 and 0-25.0 % (w/v) nacl, with optimum at ph 8.5 and 3.0 % (w/v) nacl. growth occurred at temperatures of 16-42 ºc (optimum at 28 °c). the major fatty acids were anteiso-c15 : 0 and iso-c15 : 0. mk-6 was the major respiratory quinone. the polar lipids ... | 2011 | 21498663 |
| Haloalkaliphilic heterotrophic sulfate-reducing bacteria from soda lakes and description of Desulfonatronobacter acidovorans gen. nov., sp. nov., and Desulfobulbus alkaliphilus sp. nov. | Two novel types of heterotrophic sulfate-reducing bacteria (SRB) were isolated from anoxic sediments of hypersaline soda lakes in Kulunda Steppe (Altai, Russia) using propionate as energy and carbon source. Strain APT2T was enriched and isolated with thiosulfate and strains APS1T andASS1 with sulfate as electron acceptor. APT2T was a short rod motile with a single subpolar flagellum, while APS1T/ASS1 were lemon-shaped oval rods motile with a single polar flagellum and also producing multiple th ... | 2011 | 22039002 |
| In situ identification of keratin-hydrolyzing organisms in swine manure inoculated anaerobic digesters. | Feathers, a poultry byproduct, are composed of > 90% keratin which is resistant to degradation during anaerobic digestion. In this study, four 42-L anaerobic digesters inoculated with adapted swine manure were used to investigate feather digestion. Ground feathers were added into two anaerobic digesters for biogas production, whereas another two without feathers were used as negative control. Feather degradation and enhanced methane production were recorded. Keratin-hydrolyzing organisms (KHOs) ... | 2011 | 22066513 |
| cecembia lonarensis gen. nov., sp. nov., a novel haloalkalitolerant bacterium of the family 'cyclobacteriaceae', isolated from a haloalkaline lake and emended descriptions of the genera indibacter, nitritalea and belliella. | a novel gram-staining-negative, rod shaped, non-motile bacterium, designated strain lw9t, was isolated from a water sample collected from lonar lake of buldhana district, maharastra, india. colonies and broth cultures were reddish-orange due to the presence of carotenoid pigments. strain lw9t was positive for catalase, ornithine decarboxylase and lysine decarboxylase activities and negative for gelatinase, oxidase, urease and lipase activities. the predominant fatty acids were iso-c15:0 (31.3 %) ... | 2011 | 22081718 |