| three new species in the saccharomyces sensu stricto complex: saccharomyces cariocanus, saccharomyces kudriavzevii and saccharomyces mikatae. | on the basis of genetic analysis, molecular karyotyping and sequence analyses of the 18s rrna and internal transcribed spacer (its) region, three new saccharomyces species are described, saccharomyces cariocanus (with type strain ncyc 2890t), saccharomyces kudriavzevii (with type strain ncyc 2889t) and saccharomyces mikatae (with type strain ncyc 2888t). genetic and molecular analyses did not confirm the previously observed conspecificity of saccharomyces paradoxus and s. cariocanus. the latter ... | 2000 | 11034507 |
| parallel inactivation of multiple gal pathway genes and ecological diversification in yeasts. | understanding the evolutionary relationship between genome content and ecological niche is one of the fundamental challenges of biology. the distinct physiologies of yeast species provide a window into how genomes evolve in concert with niche. although the enzymes of the well studied yeast galactose utilization pathway are present in all domains of life, we have found that multiple genes of the gal pathway are absent from four yeast species that cannot use galactose. whereas three species lack a ... | 2004 | 15381776 |
| a surprisingly large rnase p rna in candida glabrata. | we have found an extremely large ribonuclease p (rnase p) rna (rpr1) in the human pathogen candida glabrata and verified that this molecule is expressed and present in the active enzyme complex of this hemiascomycete yeast. a structural alignment of the c. glabrata sequence with 36 other hemiascomycete rnase p rnas (abbreviated as p rnas) allows us to characterize the types of insertions. in addition, 15 p rna sequences were newly characterized by searching in the recently sequenced genomes cand ... | 2005 | 15987816 |
| a homozygous diploid subset of commercial wine yeast strains. | genetic analysis was performed on 45 commercial yeasts which are used in winemaking because of their superior fermentation properties. genome sizes were estimated by propidium iodide fluorescence and flow cytometry. forty strains had genome sizes consistent with their being diploid, while five had a range of aneuploid genome sizes that ranged from 1.2 to 1.8 times larger. the diploid strains are all saccharomyces cerevisiae, based on genetic analysis of microsatellite and minisatellite markers a ... | 2006 | 16328862 |
| natural hybrids from saccharomyces cerevisiae, saccharomyces bayanus and saccharomyces kudriavzevii in wine fermentations. | several wine isolates of saccharomyces were analysed for six molecular markers, five nuclear and one mitochondrial, and new natural interspecific hybrids were identified. the molecular characterization of these saccharomyces hybrids was performed based on the restriction analysis of five nuclear genes (cat8, cyr1, gsy1, met6 and opy1, located in different chromosomes), the ribosomal region encompassing the 5.8s rrna gene and the two internal transcribed spacers, and sequence analysis of the mito ... | 2006 | 17156019 |
| genetically different wine yeasts isolated from austrian vine-growing regions influence wine aroma differently and contain putative hybrids between saccharomyces cerevisiae and saccharomyces kudriavzevii. | to evaluate the influence of the genomic properties of yeasts on the formation of wine flavour, genotypic diversity among natural saccharomyces cerevisiae strains originating from grapes collected in four localities of three austrian vine-growing areas (thermenregion: locations perchtoldsdorf and pfaffstätten, neusiedlersee-hügelland: location eisenstadt, neusiedlersee: location halbturn) was investigated and the aroma compounds produced during fermentation of the grape must of 'grüner veltliner ... | 2007 | 17484739 |
| natural populations of saccharomyces kudriavzevii in portugal are associated with oak bark and are sympatric with s. cerevisiae and s. paradoxus. | here we report the isolation of four saccharomyces species (former saccharomyces sensu stricto group) from tree bark. the employment of two temperatures (10 degrees c in addition to the more commonly used 30 degrees c) resulted in the isolation of s. kudriavzevii and s. uvarum, two species that grow at low temperatures, in addition to s. cerevisiae and s. paradoxus. a clear bias was found toward the bark of certain trees, particularly certain oak species. very often, more than one saccharomyces ... | 2008 | 18281431 |
| molecular characterization of new natural hybrids of saccharomyces cerevisiae and s. kudriavzevii in brewing. | we analyzed 24 beer strains from different origins by using pcr-restriction fragment length polymorphism analysis of different gene regions, and six new saccharomyces cerevisiae x saccharomyces kudriavzevii hybrid strains were found. this is the first time that the presence in brewing of this new type of hybrid has been demonstrated. from the comparative molecular analysis of these natural hybrids with respect to those described in wines, it can be concluded that these originated from at least t ... | 2008 | 18296532 |
| interspecies hybridization and recombination in saccharomyces wine yeasts. | the ascomycetous yeasts traditionally referred to as the saccharomyces sensu stricto complex are a group of closely related species that are isolated by a postzygotic barrier. they can easily hybridize; and their allodiploid hybrids propagate by mitotic divisions as efficiently as the parental strains, but can barely divide by meiosis, and thus rarely produce viable spores (sterile interspecies hybrids). the postzygotic isolation is not effective in allotetraploids that are able to carry out mei ... | 2008 | 18355270 |
| a multispecies-based taxonomic microarray reveals interspecies hybridization and introgression in saccharomyces cerevisiae. | a multispecies-based taxonomic microarray targeting coding sequences of diverged orthologous genes in saccharomyces cerevisiae, saccharomyces paradoxus, saccharomyces mikatae, saccharomyces bayanus, saccharomyces kudriavzevii, naumovia castellii, lachancea kluyveri and candida glabrata was designed to allow identification of isolates of these species and their interspecies hybrids. analysis of isolates of several saccharomyces species and interspecies hybrids demonstrated the ability of the micr ... | 2009 | 19054123 |
| chimeric genomes of natural hybrids of saccharomyces cerevisiae and saccharomyces kudriavzevii. | recently, a new type of hybrid resulting from the hybridization between saccharomyces cerevisiae and saccharomyces kudriavzevii was described. these strains exhibit physiological properties of potential biotechnological interest. a preliminary characterization of these hybrids showed a trend to reduce the s. kudriavzevii fraction of the hybrid genome. we characterized the genomic constitution of several wine s. cerevisiae x s. kudriavzevii strains by using a combined approach based on the restri ... | 2009 | 19251887 |
| remarkably ancient balanced polymorphisms in a multi-locus gene network. | local adaptations within species are often governed by several interacting genes scattered throughout the genome. single-locus models of selection cannot explain the maintenance of such complex variation because recombination separates co-adapted alleles. here we report a previously unrecognized type of intraspecific multi-locus genetic variation that has been maintained over a vast period. the galactose (gal) utilization gene network of saccharomyces kudriavzevii, a relative of brewer's yeast, ... | 2010 | 20164837 |
| natural hybrids of s. cerevisiae x s. kudriavzevii share alleles with european wild populations of saccharomyces kudriavzevii. | saccharomyces kudriavzevii, a yeast species described from a pair of strains isolated from decayed leaves in japan, has recently been isolated from oak barks in portugal. some data suggest that these european s. kudriavzevii populations could be closely related to the s. kudriavzevii genetic background present in natural hybrids isolated from wines and beers in different regions of europe. however, a more exhaustive study of european s. kudriavzevii natural populations is necessary to confirm th ... | 2010 | 20337723 |
| genetic identification of new biological species saccharomyces arboricolus wang et bai. | direct genetic testing for hybrid sterility unambiguously showed that the newly described yeast saccharomyces arboricolus wang et bai is reproductively isolated from saccharomyces cerevisiae, saccharomyces bayanus, saccharomyces cariocanus, saccharomyces kudriavzevii, saccharomyces mikatae and saccharomyces paradoxus and, therefore, represents a new biological species of the genus saccharomyces. combined phylogenetic analysis of the rdna repeat sequences (18s, 26s, its), nuclear act1 and mitocho ... | 2010 | 20379848 |
| genetic and phenotypic diversity of autochthonous cider yeasts in a cellar from asturias. | this paper analyses yeast diversity and dynamics during the production of asturian cider. yeasts were isolated from apple juice and at different stages of fermentation in a cellar in villaviciosa during two asturian cider-apple harvests. the species identified by its-rflp corresponded to hanseniaspora valbyensis, hanseniaspora uvarum, metschnikowia pulcherrima, pichia guilliermondii, candida parapsilosis, saccharomyces cerevisiae and saccharomyces bayanus/saccharomyces pastorianus/saccharomyces ... | 2010 | 20417399 |
| modulation of the glycerol and ethanol syntheses in the yeast saccharomyces kudriavzevii differs from that exhibited by saccharomyces cerevisiae and their hybrid. | in the last years there is an increasing demand to produce wines with higher glycerol levels and lower ethanol contents. the production of these compounds by yeasts is influenced by many environmental variables, and could be controlled by the choice of optimized cultivation conditions. the present work studies, in a wine model system, the effects of temperature, ph and sugar concentration on the glycerol and ethanol syntheses by yeasts saccharomyces cerevisiae t73, the type strain of saccharomyc ... | 2010 | 20510781 |
| monoterpene alcohols release and bioconversion by saccharomyces species and hybrids. | terpene profile of muscat wines fermented by saccharomyces species and hybrid yeasts was investigated. the amount of geraniol decreased in most wines with respect to the initial must except for saccharomyces bayanus wines. on the other hand, alpha-terpineol amount was higher in wines fermented by saccharomyces cerevisiae and hybrid yeasts. the amount of linalool was similar in all wines and comparable to the amount in the initial must. lower levels of beta-d-glucosidase activity were found in th ... | 2010 | 21176987 |
| exclusion of saccharomyces kudriavzevii from a wine model system mediated by saccharomyces cerevisiae. | this study investigated the competition and potential hybrid generation between the species saccharomyces cerevisiae and s. kudriavzevii in a wine-model environment. our main goal was to understand why s. kudriavzevii has not been found in wine fermentations whilst their hybrids are present. auxotrophic mutants (ura(-) and lys(-)) were used to favour the selection of hybrids and to specifically differentiate the two species in mixed fermentations carried out at different temperatures (17 -¦c, 24 ... | 2011 | 21381110 |
| a multiplex set of species-specific primers for rapid identification of members of the genus saccharomyces. | the saccharomyces genus (previously saccharomyces sensu stricto) formally comprises saccharomyces arboricola, saccharomyces bayanus, saccharomyces cariocanus, saccharomyces cerevisiae, saccharomyces kudriavzevii, saccharomyces mikatae, saccharomyces paradoxus and saccharomyces pastorianus. species-specific primer pairs that produce a single band of known and different product size have been developed for each member of the clade with the exception of s. pastorianus, which is a polyphyletic allop ... | 2011 | 22093682 |
| the genome sequence of the wine yeast vin7 reveals an allotriploid hybrid genome with saccharomyces cerevisiae and saccharomyces kudriavzevii origins. | the vast majority of wine fermentations are performed principally by saccharomyces cerevisiae. however, there are a growing number of instances in which other species of saccharomyces play a predominant role. interestingly, the presence of these other yeast species generally occurs via the formation of interspecific hybrids that contain genomic contributions from both s. cerevisiae and non-s. cerevisiae species. however, despite the large number of wine strains that are characterized at the geno ... | 2011 | 22136070 |
| evidence for the robustness of protein complexes to inter-species hybridization. | despite the tremendous efforts devoted to the identification of genetic incompatibilities underlying hybrid sterility and inviability, little is known about the effect of inter-species hybridization at the protein interactome level. here, we develop a screening platform for the comparison of protein-protein interactions (ppis) among closely related species and their hybrids. we examine in vivo the architecture of protein complexes in two yeast species (saccharomyces cerevisiae and saccharomyces ... | 2012 | 23300466 |