| inoculation of lambs with ovine adenovirus 5 (mastadenovirus ovi 5) strain rts-42. | twelve 1-week-old colostrum-deprived lambs inoculated with the rts-42 strain of mastadenovirus ovi 5 were killed and necropsied (2 lambs/day) on postinoculation days (pid) 2, 4, 6, 8, 12, and 21. four noninoculated lambs were killed and necropsied (2/day) on pid 6 and 12. virus was isolated from nasal secretions and feces on pid 1 to pid 6, from tracheal fluids and lung tissue of lambs necropsied on pid 2, 4, and 6, and from lung tissue from 1 lamb necropsied on pid 8. virus was not recovered fr ... | 1986 | 3008604 |
| pulmonary lesions in lambs experimentally infected with ovine adenovirus 5 strain rts-42. | twelve lambs were inoculated transtracheally and intranasally with mastadenovirus ovi 5 strain rts-42 and killed sequentially. pulmonary lesions were studied by light and electron microscopy. four lambs served as sham inoculated controls. pulmonary lesions consisted of multifocal areas of bronchiolitis and alveolitis associated with necrosis and sloughing of isolated type i and type ii alveolar epithelial cells and nonciliated bronchiolar epithelial cells. this was followed rapidly by hyperplasi ... | 1986 | 3022454 |
| preparation of sheep and cattle immunoglobulins with antibody activity by high-performance liquid chromatography. | high-performance anion-exchange chromatography (hpiec) and high-performance size-exclusion chromatography (hpsec) were used to purify serum igm and igg from sheep and cattle. pooled serum from normal cattle and sheep and serum from sheep, infected with two different viruses, were prepared for hpiec by chromatography on cm-affi-gel blue. after hpiec, fractions containing igg and igm were pooled and concentrated and further purified by hpsec. the purity of fractions from hpiec and hpsec were evalu ... | 1985 | 4030943 |